Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Touch”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 649 records · Page 36Linked to original sources
PubMed1999

Evaluation of lymph nodes in squamous cell carcinoma of the cervix: touch imprint cytology versus frozen section histology.

Metastatic involvement of pelvic lymph nodes (LNs) is the most important negative predictor of survival in early stage cervical cancer. Because the presence of nodal metastases precludes the continuance of any radical surgical procedure, an extraperitoneal LN dissection is performed and LNs are sent for frozen section (FS). As the time for routine FS is about 15 minutes per LN, the use of a faster method, cytology by touch imprint (TI), was investigated. A prospective study was performed to determine the feasibility of TI technique vs. FS. Three hundred eighteen pelvic and para-aortic LNs from 32 patients with cervical cancer of stage I-IV were bisected and submitted for FS after TI had been prepared. Twenty-nine nodes (9.1%) revealed metastatic squamous cell carcinoma (SCC) by frozen section histology. Twenty-six of these were diagnosed by TI and confirmed histologically. Reasons for the 3 false negatives included inadequate preparation or misinterpretation of the TI. Permanent histology always agreed with the frozen section result. Cytologic evaluation of pelvic LNs for SCC at the time of intraoperative consultation had a sensitivity and specificity of 90% and 100%, respectively. Touch imprints may provide a sensitive, specific, and time-efficient method to diagnose nodal metastases in cervical SCC.

Journal Article↗
PubMed2001

Gregarious behavior in desert locusts is evoked by touching their back legs.

Desert locusts in the solitarious phase were repeatedly touched on various body regions to identify the site of mechanosensory input that elicits the transition to gregarious phase behavior. The phase state of individual insects was measured after a 4-h period of localized mechanical stimulation, by using a behavioral assay based on multiple logistic regression analysis. A significant switch from solitarious to gregarious behavior occurred when the outer face of a hind femur had been stimulated, but mechanical stimulation of 10 other body regions did not result in significant behavioral change. We conclude that a primary cause of the switch in behavior that seeds the formation of locust swarms is individuals regularly touching others on the hind legs within populations that have become concentrated by the environment.

Analysis of Variance↗
PubMed2002

The clothing effect: tactile neurons in the precentral gyrus do not respond to the touch of the familiar primate chair.

Neurons in a restricted zone in the precentral gyrus of macaque monkeys respond to tactile, visual, and auditory stimuli. The tactile receptive fields of these multimodal cells are usually located on the face, arm, or upper torso. In the present study, in awake monkeys sitting in a primate chair, the neurons responded to a tactile probe touching the skin within the tactile receptive field. However, the same neurons did not respond when the tactile receptive field was touched by the primate chair, to which the monkey was habituated.

Animals↗
PubMed1996

P2Y1 purinergic receptors in sensory neurons: contribution to touch-induced impulse generation.

Somatic sensation requires the conversion of physical stimuli into the depolarization of distal nerve endings. A single cRNA derived from sensory neurons renders Xenopus laevis oocytes mechanosensitive and is found to encode a P2Y1 purinergic receptor. P2Y1 mRNA is concentrated in large-fiber dorsal root ganglion neurons. In contrast, P2X3 mRNA is localized to small-fiber sensory neurons and produces less mechanosensitivity in oocytes. The frequency of touch-induced action potentials from frog sensory nerve fibers is increased by the presence of P2 receptor agonists at the peripheral nerve ending and is decreased by the presence of P2 antagonists. P2X-selective agents do not have these effects. The release of ATP into the extracellular space and the activation of peripheral P2Y1 receptors appear to participate in the generation of sensory action potentials by light touch.

Adenosine Triphosphate↗
PubMed2000

Touch-down and take-off characteristics of the long jump performance of world level above- and below-knee amputee athletes.

The aims of this study were to establish the take-off characteristics of long jump performance of disabled amputee athletes, and to establish to what extent amputee athletes conform to a model of performance defined for elite able-bodied athletes. The jumps of 8 male below-knee (trans-tibial) and 8 male above-knee (trans-femoral) amputee athletes who competed in the finals of the long jump at the 1998 World Disabled Championships were recorded in the sagittal plane on video (50 Hz). Approach speed was measured using a laser Doppler system. The best jump for each athlete was digitized, and kinematic data from the key instants of touch-down (TD), maximum knee flexion (MKF) and take-off (TO) were obtained. Amputees demonstrated a lower approach speed and jumped less far than able-bodied athletes although below-knee amputees performed better than above-knee amputees. For each amputee group there was a significant (p < 0.05) linear relationship between approach speed and distance jumped. With the exception of their slower horizontal speed and greater negative vertical speed at touch-down, below-knee amputees demonstrated characteristics of technique that were similar to elite able-bodied long jumpers. Above-knee amputees at touchdown had a more upright trunk, smaller hip and knee angles and consequently a smaller leg angle. This was attributed to the difficulty of taking off on the last stride on the prosthetic limb. Consequently, above-knee amputees were less able to gain vertical velocity during the compression (TD-MKF) phase, but were able to compensate for this by using a greater hip range of motion during the extension (MKF-TO) phase. It was concluded that below-knee amputees displayed the same basic jumping technique as elite able-bodied long jumpers, but above-knee amputees did not. These findings have implications for the training and technical preparation of amputee long jumpers.

Amputees↗
PubMed2002

Touch-typing VDU operation: workstation adjustment, working posture and workers' perceptions.

At a VDU workstation professional touch-typing operators worked at eight different combined adjustments of visual target height and chair backrest inclination. Working posture, workers' perceptions and work performance were measured. Two conclusions were drawn. First, in order to minimize the load on the musculoskeletal system for touch-typing VDU operators, the gaze inclination to a visual target (screen, document) should be 6-9 degrees (range 0-15 degrees ) below the horizontal and, second, the gaze inclination recommended is independent of sitting posture (that is with the backrest between upright and 15 degrees inclined backwards).

Adult↗
PubMed1996

Cutaneous overexpression of NT-3 increases sensory and sympathetic neuron number and enhances touch dome and hair follicle innervation.

Target-derived influences of nerve growth factor on neuronal survival and differentiation are well documented, though effects of other neurotrophins are less clear. To examine the influence of NT-3 neurotrophin overexpression in a target tissue of sensory and sympathetic neurons, transgenic mice were isolated that overexpress NT-3 in the epidermis. Overexpression of NT-3 led to a 42% increase in the number of dorsal root ganglia sensory neurons, a 70% increase in the number of trigeminal sensory neurons, and a 32% increase in sympathetic neurons. Elevated NT-3 also caused enlargement of touch dome mechanoreceptor units, sensory end organs innervated by slowly adapting type 1 (SA1) neurons. The enlarged touch dome units of the transgenics had an increased number of associated Merkel cells, cells at which SA1s terminate. An additional alteration of skin innervation in NT-3 transgenics was an increased density of myelinated circular endings associated with the piloneural complex. The enhancement of innervation to the skin was accompanied by a doubling in the number of sensory neurons expressing trkC. In addition, measures of nerve fibers in cross-sectional profiles of cutaneous saphenous nerves of transgenics showed a 60% increase in myelinated fibers. These results indicate that in vivo overexpression of NT-3 by the epidermis enhances the number of sensory and sympathetic neurons and the development of selected sensory endings of the skin.

Animals↗
PubMed2003

A multicenter study of the accuracy of the One Touch Ultra home glucose meter in children with type 1 diabetes.

Data are not readily available on the accuracy of one of the most commonly used home blood glucose meters, the One Touch Ultra (LifeScan, Milpitas, California). The purpose of this report is to provide information on the accuracy of this home glucose meter in children with type 1 diabetes. During a 24-h clinical research center stay, the accuracy of the Ultra meter was assessed in 91 children, 3-17 years old, with type 1 diabetes by comparing the Ultra glucose values with concurrent reference serum glucose values measured in a central laboratory. The Pearson correlation between the 2,068 paired Ultra and reference values was 0.97, with the median relative absolute difference being 6%. Ninety-four percent of all Ultra values (96% of venous and 84% of capillary samples) met the proposed International Organisation for Standardisation (ISO) standard for instruments used for self-monitoring of glucose when compared with venous reference values. Ninety-nine percent of values were in zones A + B of the Modified Error Grid. A high degree of accuracy was seen across the full range of glucose values. For 353 data points during an insulin-induced hypoglycemia test, the Ultra meter was found to have accuracy that was comparable to concurrently used benchmark instruments (Beckman, YSI, or i-STAT); 95% and 96% of readings from the Ultra meter and the benchmark instruments met the proposed ISO criteria, respectively. These results confirm that the One Touch Ultra meter provides accurate glucose measurements for both hypoglycemia and hyperglycemia in children with type 1 diabetes.

Adolescent↗
PubMed1982

The immunoperoxidase method for rapid diagnosis of Herpes simplex encephalitis (HSE) using touch preparations.

Ten rats were inoculated intracerebrally with Herpes simplex virus type I to study the possibility of establishing a rapid diagnosis of Herpes simplex encephalitis through examination of touch preparations of brain tissue stained by the immunoperoxidase method. All except one of the animals died within four days of the developing encephalitis. The surviving animal was killed on the fourth day. Touch preparations were shortly fixed in 4% buffered formalin, cold acetone, or alcohol. The indirect immunoperoxidase method was used to identify viral antigen. The authors found that formalin serves as a good fixative and sensitivity as well as specificity is high. The diagnosis could be made within 4 hours. The cellular morphology is retained. The reaction product is stable and background staining no problem. The results of this study imply to use this method also for rapid diagnosis of brain biopsy tissue in human infections.

Animals↗
PubMed2004

Pregnancy with frozen-thawed and fresh testicular biopsy after motile and immotile sperm microinjection, using the mechanical touch technique to assess viability.

BACKGROUND: There are few reports of pregnancy using immotile sperm, and none using a purely mechanical assessment of viability. METHODS: In this pilot study, we retrospectively analysed 66 cycles in 61 patients with determinant male factor, recording rates of fertilization, implantation, normal pregnancy and take-home babies achieved with ICSI. Sperm selection was based on morphologically normal appearance under the inverted microscope. Viability of immotile spermatozoa was assessed by the mechanical touch technique to observe tail flexibility and tail shape recovery. RESULTS: Of 17 ICSI cycles using frozen-thawed testicular sperm, six microinjected with immotile and 11 with motile sperm, we achieved fertilization rates of 65.7 and 74.3%, respectively, and five pregnancies (two and three, respectively). Of 49 ICSI cycles using fresh testicular sperm, 10 microinjected with immotile and 39 with motile sperm, we achieved fertilization rates of 73.4 and 64.4%, respectively, and 12 pregnancies (three and nine, respectively). CONCLUSIONS: Immotile (fresh and frozen-thawed) testicular sperm of normal morphological appearance can be used to achieve clinical pregnancy with ICSI. Our results strongly suggest that immotile sperm viability can be assessed by the mechanical touch technique.

Adult↗
PubMed1991

Direct sequencing from touch preparations of human carcinomas: analysis of p53 mutations in breast carcinomas.

A new technique for characterizing somatic mutations in very small samples of cellularly heterogeneous human cancer tissue was developed and tested using mutations in the p53 gene in breast carcinomas as a model system. The technique combines touch preparation of specimens to obtain homogeneous clusters of carcinoma cells free of normal cells with a nested pair of polymerase chain reaction (PCR) amplifications of DNA to increase the amount of target gene sequence sufficiently to permit direct sequencing of the p53 gene. Touch preparations of fresh or previously frozen tissue from human adenocarcinomas derived from several organs were stained, and clusters of 10-50 malignant cells were transferred by pipette into microfuge tubes for PCR amplification. Exons 5-9 of the p53 gene, which contain the major mutational hot spots associated with most human cancers, were sequenced by the following steps: 1) two rounds of PCR amplification using DNA Taq polymerase and two sets of oligonucleotide primers, the second set being nested within the segment amplified by the first set and having attached T7 and SP6 phage promoter sequences, 2) transcription of the amplified DNA sequences with T7 and SP6 RNA polymerases, and 3) dideoxy sequencing of single-stranded RNA transcripts with reverse transcriptase and with additional oligonucleotide primers to achieve specificity for this unique region of the genome. The utility of this approach is illustrated by our success in detecting and analyzing point mutations in cell clusters from four of 11 primary adenocarcinomas of the human breast.

Adenocarcinoma↗
PubMed1987

Toe-touch test. A measure of its validity.

The primary purpose of this study was to investigate the relationships between the vertical fingertip-floor distance (FFD) and the hip and vertebral components of trunk flexion during the toe-touch test. Measurements were taken from lateral photographs of each subject standing in the erect and maximally flexed positions. The subjects were healthy young men (n = 16) and women (n = 17). The mean FFD was 3 cm below floor level (-3 cm) with a standard deviation of 8 cm. Reliability of this measure was indicated by a test-retest correlation coefficient of .97. The FFD was a measure of both trunk (r = -.85) and hip flexion (r = -.79), but not of vertebral flexion (r = .10). At maximum trunk flexion, therefore, FFD is mainly a measure of the extensibility of the hamstring muscles that limit hip flexion when the knees remain extended. The toe-touch test was determined to be unsuitable for monitoring the effect of a treatment regimen to improve vertebral mobility because the partial correlation coefficient between vertebral flexion and FFD was -.64 (p less than .01), with the effect of hip flexion held constant. Although this correlation is significant, it means that less than half of the variation in FFD can be explained by changes in vertebral mobility. For this group of young adults, no significant correlations existed between the FFD and limb length and abdominal girth at maximum trunk flexion.

Adult↗
PubMedDate not supplied

Audio computerized self-report interview use in prenatal clinics: audio computer-assisted self interview with touch screen to detect alcohol consumption in pregnant women: application of a new technology to an old problem.

Computer interviewing to obtain sensitive information is not a new concept. However, concerns about the acceptance of computers in disadvantaged populations with potentially low literacy led us to combine audio- and touch-screen technologies with an audio computerized self-report interview to obtain information about alcohol use. This study evaluated acceptance and ease of use by a disadvantaged population of pregnant women in the District of Columbia. Patients attending an initial visit at prenatal clinics answered questions anonymously about their consumption of alcoholic beverages and other personal information. The questionnaire was programmed on a laptop computer. The computer administered the recorded questions via earphones, as well as displayed them on the screen, and patients answered by touching the computer screen. Results were immediately available. A total of 507 women were interviewed, who were primarily African American, non-Hispanic, and never married. Nearly 24% did not complete a high school education, 43% were unemployed, and 30% received public assistance. Most of the women (59%) used computers occasionally (a few days a month) or never. Nearly all patients (96%) reported that the computer was not difficult to use, and approximately 90% liked answering the questions by computer. The study demonstrates that using computers to screen for alcohol use in disadvantaged pregnant populations is feasible and acceptable to the patients.

Adolescent↗
PubMed2006

Fluorescence in situ hybridization (FISH) on touch preparations: a reliable method for detecting loss of heterozygosity at 1p and 19q in oligodendroglial tumors.

Combined loss of heterozygosity (LOH) on 1p and 19q is reported in 50% to 90% of oligodendroglial tumors and has emerged as a strong and favorable prognostic factor. Fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) are the most widely used techniques. The aim of this study was to evaluate the reliability of FISH to predict LOH at 1p and 19q when performed on touch preparations from 40 oligodendroglial tumors, even if the majority of the nuclei showed chromosomal imbalance. PCR was used as the gold standard. The presence of none or one target signal was reported as FISH-LOH, whereas all other losses were defined as FISH-imbalance. The sum of nuclei with FISH-LOH and imbalance was calculated in each case (FISH-sum) and cut-off values were defined as the mean FISH-sum value in controls plus 3 standard deviations; 27.7% for 1p and 33.2% for 19q. These corresponded well with the optimal cut-off values for our data, calculated using the minimum error rate classification procedure (35.6% for 1p and 33.1% for 19q). Concurrent FISH and PCR results were encountered in 95% for 1p and 87.5% for 19q. FISH-sum was the best and simplest discriminating variable for correct classification of LOH status. Under these conditions, even a dominant population of nuclei showing FISH-imbalance represented an LOH status in the tumor cells. FISH on touch preparations is a quick and reliable method for 1p/19q testing, does not require normal DNA and can be easily performed in an immunohistochemistry unit.

Adult↗
PubMedDate not supplied

Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers.

We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI 17-321) and 17p13.3 (D17S34), and a combination of the probes designed for 1q12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95% (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93% (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90%) and five (100%) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.

Adenocarcinoma↗
PubMed1984

The use of touch-sensitive screen in interactive morphometry.

We propose the use of a touch-sensitive screen as a tool to facilitate acquisition of data both in point counting and morphometric planimetry. It obviates the use of a 'correspondence image' required with many planimetric devices in which the means for data acquisition and display are separate. The touch-sensitive screen offers operational advantages over the light pen. The characteristics of this new graphical input device, its assembly and basic operational considerations are introduced and discussed.

Computers↗
PubMed2005

Audiovisual touch-screen computer-assisted self-interviewing for donor health histories: results from two years experience with the system.

BACKGROUND: The donor history interview is an important aspect of blood safety, in part designed to identify unsuitable donors who may present a risk to blood recipients. There is evidence from behavioral science literature that use of computer-assisted interviewing may be superior to face-to-face (FTF) and paper techniques in eliciting sensitive behavioral information of interest to blood collection facilities. STUDY DESIGN AND METHODS: Audiovisual touch-screen computer-assisted donor self-interviewing with the AABB Uniform Donor History Questionnaire was deployed for routine use in a regional blood center replacing FTF interviews. Donor and staff perception and satisfaction surveys were performed to assess acceptance of the system. Time studies of automated and manual methods were conducted. Rates of deferral of first-time donors for high-risk behaviors and rates of errors and omissions on donor interviewing for the two systems were tabulated and compared. RESULTS: Donors and staff strongly preferred the automated system in all dimensions assessed. Donor time increased by 4 minutes but staff time declined by 5 minutes per interview. Identification of high-risk behaviors among first-time donors significantly increased. Rates of errors and omissions on donor history forms identified at audit were reduced. CONCLUSIONS: Both blood donors and collections staff enthusiastically accepted the automated donor interviewing system. A well-designed audiovisual touch-screen donor self-interviewing system is superior to face-to-face interviewing and most likely more effective than paper interviewing.

Attitude of Health Personnel↗
PubMed1982

Sensitivity of touch corpuscles in vitamin A-deficient rats.

Fifty percent of a population of thirty weanling rats were fed a vitamin A-deficient diet. The remaining 50% were fed a control diet. In comparison with the control group, the rate of growth started to slow down in the vitamin A-deficient animals around day 33. Single unit recordings were made from sensory nerve fibres supplying slowly adapting type I cutaneous mechanoreceptors (touch corpuscles). Touch corpuscles were stimulated repetitively with low force mechanical stimuli. Receptors in vitamin A-deficient animals showed clearly reduced nervous responses compared with receptors in control animals. Most receptors remained responsive even after prolonged repetitive stimulation.

Animals↗