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At least 649 records · Page 36Linked to original sources
PubMed1995

Sets of EcoRI fragments containing ribosomal RNA sequences are conserved among different strains of Listeria monocytogenes.

To classify Listeria monocytogenes using taxonomic characters derived from the rRNA operons and their flanking sequences, we studied a sample of 1346 strains within the taxon. DNA from each strain was digested with a restriction endonuclease, EcoRI. The fragments were separated by gel electrophoresis, immobilized on a membrane, and hybridized with a labeled rRNA operon from Escherichia coli. The pattern of bands, positions, and intensities of hybridized fragments were electronically captured. Software was used to normalize the band positions relative to standards, scale the signal intensity, and reduce the background so that each strain was reproducibly represented in a data base as a pattern. With these methods, L. monocytogenes was resolved into 50 pattern types differing in the length of at least one polymorphic fragment. Pattern types representing multiple strains were recorded as the mathematical average of the strain patterns. Pattern types were arranged by size polymorphisms of assigned rRNA regions into subsets, which revealed the branching genetic structure of the species. Subtracting the polymorphic variants of a specific assigned region from the pattern types and averaging the types within each subset resulted in reduced sets of conserved fragments that could be used to recognize strains of the species. Pattern types and reduced sets of conserved fragments were conserved among different strains of L. monocytogenes but were not observed in total among strains of other species.

Base Sequence↗
PubMed1981

Calcium specifically stimulates prolactin synthesis and messenger RNA sequences in GH3 cells.

Addition of CaCl2 (0.4 mM) to GH3 cells incubated in a serum-free, Ca2+-free medium increased relative prolactin synthesis (prolactin synthesis/total protein synthesis) 7- to 150-fold by 3-4 days, but had no effect on relative growth hormone synthesis. Half-maximal and maximal stimulation of relative prolactin synthesis occurred at about 0.07 and 0.2 mM CaCl2, respectively. Neither MgCl2 (0.4 mM) nor KCl 0.4 mM) affected relative prolactin synthesis. 32P-labeled rat prolactin and growth hormone cDNA plasmids were employed to measure by RNA gel blot hybridization changes in cytoplasmic levels of the corresponding mRNA sequences. Both prolactin mRNA sequence levels and translatable prolactin mRNA increased in parallel with the CaCl2-induced increase in relative prolactin synthesis. CaCl2 increased prolactin mRNA sequences by 7- to 200-fold, without affecting growth hormone mRNA sequence levels. Dexamethasone (100 nM) decreased prolactin mRNA sequences in cells incubated with CaCl2, antagonized the CaCl2-induced increases in prolactin mRNA sequences independently of the CaCl2 concentration.

Animals↗