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PubMed1995

Erroneous mass spectrometer readings caused by desflurane and sevoflurane.

OBJECTIVE: Medical mass spectrometers are configured to detect and measure specific respiratory and anesthetic gases. Unrecognized gases entering these systems may cause erroneous readings. We determined how the Advantage 1100 (Perkin-Elmer, now Marquette Gas Systems, Milwaukee, WI) and PPG-SARA (PPG Biomedical Systems, Lenexa, KS) systems that were not configured to measure desflurane or sevoflurane respond to increasing concentrations of these new potent volatile anesthetic agents. METHODS: Desflurane 0% to 18% in 3% increments or sevoflurane 0% to 7% in 1% increments in 5-L/min oxygen was delivered to the Advantage and PPG-SARA mass spectrometry systems. For each concentration of each agent, the displayed gas analysis readings and uncompensated collector plate voltages were recorded. RESULTS: The Advantage 1100 system read both desflurane and sevoflurane mainly as enflurane and, to a lesser extent, as carbon dioxide and isoflurane. For enflurane(E) readings < 9.9%, the approximate relationships are: %Desflurane = 1.6E; %Sevoflurane = 0.3E. These formulas do not apply if E > 9.9% because of saturation of the summation bus. PPG-SARA read desflurane mainly as isoflurane(I) and, to a lesser extent, as nitrous oxide. PPG-SARA read sevoflurane mainly as enflurane(E) and, to a lesser extent, as nitrous oxide and halothane. The approximate relationships are: %Desflurane = 1.11 (for I < 9%); %Sevoflurane = 2.1E. CONCLUSIONS: Advantage 1100 and PPG-SARA systems not configured for desflurane or sevoflurane display erroneous anesthetic agent readings when these new agents are sampled. Advantage 1100 also displays falsely elevated carbon dioxide readings when desflurane is sampled.

Anesthetics↗
PubMed2000

The filmless radiology reading room: a survey of established picture archiving and communication system sites.

The purpose of this study was to survey radiologists experienced in soft-copy diagnosis using computer workstations about their current reading room environment, their impressions of the efficacy of their reading room design, and their recommendations based on their experience for improvement of the soft-copy reading environment. Surveys were obtained from radiologists at seven sites representing three major picture archiving and communication system (PACS) vendors throughout the world that have had extensive experience with soft-copy interpretation of radiology studies. The radiologists filled out a detailed survey, which was designed to assess their current reading room environment and to provide them with the opportunity to make suggestions about improvement of the PACS reading rooms. The survey data were entered into a database and results were correlated with multiple parameters, including experience with PACS, types of modalities interpreted on the system, and number of years of experience in radiology. The factors judged to be most important in promoting radiologist productivity were room lighting, monitor number, and monitor brightness. Almost all of the radiologists indicated that their lighting source was from overhead rather than indirect or portable light sources. Approximately half indicated they had the capability of dimming the brightness of the overhead lighting. Most radiologists indicated that they were able to adjust room temperature but that they did not have individual temperature controls at their workstations. The radiologists indicated that the most troublesome sources of noise included background noise, other radiologists, and clinicians much more than noise from computer monitors, technologists, or patients. Most radiologists did not have chairs that could recline or arm rests. Most did have wheels and the capability to swivel, both of which were judged important. The majority of chairs also had lumbar support, which was also seen to be important. Radiologists commonly adjusted room lighting and their reading chair, but rarely adjusted room temperature or monitor brightness. The median number of hours spent at the workstation before taken a "break" was 1.5. Common recommendations to improve the room layout included compartmentalization of the reading room and availability of the hospital/radiology information system at each workstation. The survey data suggest several areas of potential improvement based on radiologists' experience. Optimization of soft-copy reading room design is likely to result in decreased fatigue and increased productivity.

Attitude of Health Personnel↗
PubMed1985

Spatial-frequency-dependent visible persistence and specific reading disability.

Visible persistence duration for sine-wave gratings was measured in 9-year-old normal and specific-reading-disabled children. Experiment 1 investigated the influence of stimulus duration on visible persistence. The results demonstrated a Reading Group X Spatial Frequency interaction with disabled readers showing a smaller increase in persistence duration with increasing spatial frequency than controls. This interaction was greatest with stimulus durations longer than 80 msec. In Experiments 2a and 2b persistence was measured across a range of contrasts from .1 to .5. The stimulus durations employed were 100 msec in Experiment 2a and 300 msec in Experiment 2b. In both experiments, increasing contrast decreased persistence duration at 2 and 12 cycles per degree (c/deg) for the control group. In the specific-reading-disabled group, however, contrast had little effect on the persistence of 2-c/deg gratings in either experiment. In Experiment 2a the persistence of the 12-c/deg grating decreased with increasing contrast for both groups. In Experiment 2b contrast had significantly less effect on persistence duration in the specific-reading-disabled group, however, contrast had little effect on the persistence of 2-c/deg ratings in either experiment. In Experiment 2b contrast had significantly less effect on persistence duration in the specific-reading-disabled group than in the control group at 12 c/deg. Consequently, contrast had less effect on persistence in specific-reading-disabled children than in normal readers, especially at durations longer than the "critical duration" for each spatial frequency. Experiment 3 extended this finding to gratings with spatial frequencies of 4 and 8 c/deg. These results indicate a difference between normal and specific-reading-disabled children in cortical visible persistence. Two scores of visual processing were derived from the above experiments. On these scores the reading-disabled children were divided into Visual Disabled Readers (approximately 70%--eight subjects) and Nonvisual Disabled Readers (approximately 30%--four subjects). The percentages of disabled readers in each category remained constant when the sample size was increased to 61 normal and disabled readers.

Attention↗
PubMed2006

Methylphenidate improves reading performance in children with attention deficit hyperactivity disorder and comorbid dyslexia: an unblinded clinical trial.

Attention Deficit Hyperactivity Disorder (ADHD) and dyslexia are frequently co-occurring disorders. Although methylphenidate (MPH) is the primary treatment for ADHD, the effect on reading in children with these comorbid problems is not yet known. This study was an unblinded clinical trial to evaluate the reading performance before and after treatment with MPH. Reading performance was compared with General Linear Model repeated measures between three groups: (1) an experimental group of children with both ADHD and dyslexia (N = 24), (2) a control group of children with ADHD (N = 9) and (3) a control group of children with dyslexia (N = 10). MPH improved reading performance significantly stronger in the experimental group than in the control groups; the number of correctly read words increased to a larger extent. In conclusion, MPH proved to be an aid in the reading process of children with ADHD and comorbid dyslexia by improving the learning conditions, but MPH cannot cure the reading disorder. Future research should study the effect of MPH on reading in a double-blind clinical trial.

Adolescent↗
PubMed2005

Clinician versus reading center assessment of cytomegalovirus retinitis lesion size.

PURPOSE: To compare clinician and fundus photograph reading center assessments of the cytomegalovirus (CMV) retinitis area and change in the CMV retinitis area over time, and to investigate how these assessments correlate with the visual field (VF) of eyes with CMV retinitis. DESIGN: Analysis of pooled data from 2 multicenter randomized clinical trials and 1 prospective multicenter epidemiologic study. PARTICIPANTS: Ninety-five eyes of 79 patients. At baseline, each eye had CMV retinitis restricted to zone 1 and/or zone 2 (approximately the photographable postequatorial retina), as assessed by the evaluating clinician. METHODS: Comparison of CMV retinitis area, change in area over time as assessed by clinicians and a fundus photograph reading center, and correlation of these assessments with VF measurement. MAIN OUTCOME MEASURES: Cytomegalovirus retinitis area, change in CMV retinitis area over time, and VF score. RESULTS: Baseline assessments of the mean retinitis area were, by clinicians, 12.8% of the total retinal area and, by the reading center, 6.3% of the total retinal area (P<0.001). There was a positive correlation between clinician and reading center assessments of retinitis area at baseline (rho = 0.77 and P<0.0001 by Pearson correlation and rho = 0.54 and P<0.001 by concordance). Both clinician and reading center size measures correlated negatively with VF (Spearman correlation rhos = -0.38 and -0.52, respectively; P<0.001 each). Mean changes in area over a 3-month interval were, by clinicians, +1.2% and, by the reading center, +1.1% (P = 0.68). Regression analysis showed a positive concordance (rho = 0.42, P<0.001). Change in VF over a 3-month interval did not correlate with change in retinitis area as assessed by clinicians or the reading center. CONCLUSIONS: Awareness of the similarities and differences between clinician and reading center assessments of CMV retinitis area should permit clinicians to apply research data to clinical practice more effectively. Clinician assessment of retinitis area correlates negatively with VF, a clinically meaningful visual outcome in patients with CMV retinitis.

AIDS-Related Opportunistic Infections↗
PubMed2006

Does otitis media in early childhood affect reading performance in later school years?

OBJECTIVE: To evaluate reading performance in children with recurrent otitis media during childhood once middle-ear diseases have resolved and the children grew older. STUDY DESIGN AND SETTING: Seventy-five children with middle-ear problems during childhood and poor reading performance in first grades and 60 healthy controls free of middle ear diseases were enrolled in the study. All children underwent an otologic and audiologic evaluation followed by reading tests. RESULTS: Reading performance was not affected once otitis media had been cured and hearing restored; reading scores of the healed children were almost the same as those of the otitis-free children (3.39% and 3.1%, respectively). Children who still suffer from middle-ear problems and hearing loss had an average percentage of reading mistakes of 7.3%. CONCLUSIONS AND SIGNIFICANCE: Once children are cured from their middle-ear diseases and hearing is restored, previously noted reading impairments are no longer evident. Reading is still impaired among children who continue to suffer from middle-ear problems and hearing loss even when they grow older. EBM RATING: B-2b.

Case-Control Studies↗
PubMed1988

Reliability and reproducibility of nonstress test readings.

The nonstress test is commonly used as a test of fetal well-being, despite the fact that both false-positive and false-negative results are known to occur. Although some of the errors are due to biologic variability among fetuses, some may be due to variability of reading the test. The purpose of the present study was to assess the reliability and reproducibility of nonstress test readings. Fifty nonstress test monitor strips were read by five perinatologists on two occasions, for a total of 500 readings. Nonstress tests were read as reactive, equivocal, nonreactive, or technically unsatisfactory. Of the 50 tracings, only 11 had identical readings on all 10 occasions, and readers had different readings for the first and second readings 28% of the time. Estimates of interobserver and intraobserver agreement ranged from 0.41 to 0.55. These results indicate that there is considerable interobserver and intraobserver variability that can be expected to have a serious adverse effect on the sensitivity of the nonstress test.

Female↗
PubMed2025

Severus detects somatic structural variation and complex rearrangements in cancer genomes using long-read sequencing.

For the detection of somatic structural variation (SV) in cancer genomes, long-read sequencing is advantageous over short-read sequencing with respect to mappability and variant phasing. However, most current long-read SV detection methods are not developed for the analysis of tumor genomes characterized by complex rearrangements and heterogeneity. Here, we present Severus, a breakpoint graph-based algorithm for somatic SV calling from long-read cancer sequencing. Severus works with matching normal samples, supports unbalanced cancer karyotypes, can characterize complex multibreak SV patterns and produces haplotype-specific calls. On a comprehensive multitechnology cell line panel, Severus consistently outperforms other long-read and short-read methods in terms of SV detection F1 score (harmonic mean of the precision and recall). We also illustrate that compared to long-read methods, short-read sequencing systematically misses certain classes of somatic SVs, such as insertions or clustered rearrangements. We apply Severus to several clinical cases of pediatric leukemia/lymphoma, revealing clinically relevant cryptic rearrangements missed by standard genomic panels.

Humans↗
PubMed1999

Revisiting the issue: can the reading for serologic reactivity following 37 degrees C incubation be omitted?

BACKGROUND: Omitting the 37 degrees C reading from screening tests for unexpected antibodies results in failure to detect some Rh, K, and Jk agglutinins of potential significance (wanted positives). However, this measure avoids unwanted positive tests due to cold agglutinins. STUDY DESIGN AND METHODS: Using data from prior publications, actual risk calculations (ARCs) were made to predict the risk of eliminating the 37 degrees C reading, pretransfusion direct antiglobulin test (DAT), and routine indirect antiglobulin crossmatch (IAT-XM). ARCs used the equation: wanted positives missed x 0.34 (or 0.80) x 5 x percent antigen-positive, where 0.34 = percent of patients transfused (ARCs for 37 degrees C reading and DAT); 0.80 = percent of crossmatched patients transfused (ARCs for IAT-XM); 5 = average number of units transfused. Following elimination of the 37 degrees C reading, the impact of this change on patient care was monitored. Antibody detection and identification data and transfusion reaction reports for 6 months after the change were reviewed. Recently transfused patients with new antibodies were evaluated for immune hemolysis by review of clinical and laboratory data. The findings were compared with those from the same dates of the preceding year. RESULTS: The risk of transfusing incompatible blood by eliminating the DAT, IAT-XM, and 37 degrees C reading is approximately 1:13,000, 1:2,000, and 1:2,400 units transfused, respectively. The cumulative risk from eliminating all three tests is approximately. 1 :1,000 units. With respect to the 37 degrees C reading, there were no differences between the pre-change and post-change study periods in the incidence of reported transfusion reactions or cases of immune hemolysis associated with newly formed antibodies. However, unwanted positive tests decreased from 162 to 61 following elimination of the 37 degrees C reading. This represents a decrease of 20 percent in the number of samples requiring antibody identification annually. CONCLUSIONS: Eliminating the 37 degrees C reading from pretransfusion antibody screening tests imposes less risk than omitting the routine IAT-XM, and it avoids the time and costs of evaluating unwanted positive tests, thus reducing expenditures and delays in patient care.

Actuarial Analysis↗
PubMed2005

Detection rate and efficiency of lymph node assessment with axial and coronal image reading based on 16 row multislice CT of the neck.

PURPOSE: Multislice CT (MSCT) has the advantage of isotropic volumetric data acquisition which allows high resolution data reconstruction in the axial and coronal plane. We evaluate the accuracy and efficiency of coronal reconstruction compared to axial reconstructions of a routinely performed CT scan exemplary in neck lymph node assessment performed on a 16 row MSCT. MATERIAL AND METHODS: Contrast enhanced neck MSCT of 24 patients with known lymphoma were evaluated prospectively for lymph node assessment. 4 blinded readers evaluated the axial and coronal reconstructions of the same patient. Neck lymph nodes larger than 10 mm were evaluated by their anatomical region (deep jugular chain, submandibular, nuchal). Time for axial and coronal image evaluation was assessed. Detection rate was compared with consensus reading as gold standard. RESULTS: In consensus reading 169 enlarged lymph nodes in the deep jugular chain were found. Detection rate for axial image interpretation was 36.1 % with 54.9 % in coronal reading. Assessing the submandibular lymph nodes (n = 45) axial interpretation revealed 53.9 % with 36.1 % in coronal reading. Evaluation time for axial reading was in all but one reader significantly longer (mean 176 seconds) than in coronal reading (mean 129 seconds). CONCLUSION: Coronal image reading improves the detection rate of cranio-caudal oriented structures. Considering representatively neck lymph nodes in the deep jugular chain the image interpretation time is significantly reduced. Still axial reading remains necessary for assessing axially oriented structures such as the submandibular region in the neck.

Adult↗
PubMed2025

De novo clustering of large long-read transcriptome datasets with isONclust3.

MOTIVATION: Long-read sequencing techniques can sequence transcripts from end to end, greatly improving our ability to study the transcription process. Although there are several well-established tools for long-read transcriptome analysis, most are reference-based. This limits the analysis of organisms without high-quality reference genomes and samples or genes with high variability (e.g. cancer samples or some gene families). In such settings, analysis using a reference-free method is favorable. The computational problem of clustering long reads by region of common origin is well-established for reference-free transcriptome analysis pipelines. Such clustering enables large datasets to be split roughly by gene family and, therefore, an independent analysis of each cluster. There exist tools for this. However, none of those tools can efficiently process the large amount of reads that are now generated by long-read sequencing technologies. RESULTS: We present isONclust3, an improved algorithm over isONclust and isONclust2, to cluster massive long-read transcriptome datasets into gene families. Like isONclust, isONclust3 represents each cluster with a set of minimizers. However, unlike other approaches, isONclust3 dynamically updates the cluster representation during clustering by adding high-confidence minimizers from new reads assigned to the cluster and employs an iterative cluster-merging step. We show that isONclust3 yields results with higher or comparable quality to state-of-the-art algorithms but is 10-100 times faster on large datasets. Also, using a 256&#x2009;Gb computing node, isONclust3 was the only tool that could cluster 37 million PacBio reads, which is a typical throughput of the recent PacBio Revio sequencing machine. AVAILABILITY AND IMPLEMENTATION: https://github.com/aljpetri/isONclust3.

Algorithms↗
PubMed2025

Genome size estimation from long read overlaps.

MOTIVATION: Accurate genome size estimation is an important component of genomic analyses such as assembly and coverage calculation, though existing tools are primarily optimized for short-read data. RESULTS: We present LRGE, a novel tool that uses read-to-read overlap information to estimate genome size in a reference-free manner. LRGE calculates per-read genome size estimates by analysing the expected number of overlaps for each read, considering read lengths and a minimum overlap threshold. The final size is taken as the median of these estimates, ensuring robustness to outliers such as reads with no overlaps. Additionally, LRGE provides an expected confidence range for the estimate. We validate LRGE on a large, diverse bacterial dataset and confirm it generalizes to eukaryotic datasets. On bacterial genomes, LRGE outperforms k-mer-based methods in both accuracy and computational efficiency and produces genome size estimates comparable to those from assembly-based approaches, like Raven, while using significantly less computational resources. AVAILABILITY AND IMPLEMENTATION: Our method, LRGE (Long Read-based Genome size Estimation from overlaps), is implemented in Rust and is available as a precompiled binary for most architectures, a Bioconda package, a prebuilt container image, and a crates.io package as a binary (lrge) or library (liblrge). The source code is available at https://github.com/mbhall88/lrge and an archive at https://doi.org/10.5281/zenodo.17183812 under an MIT license.

Genome Size↗
PubMed1990

Differential reading recovery in patients with severe to moderate closed head injury.

A differential recovery was seen when alternate forms of a nationally standardized test of Reading Vocabulary, Literal Reading Comprehension and Inferential Reading Comprehension was administered serially to 10 consecutive closed head injury admissions to a university rehabilitation hospital. Inferential Reading Comprehension was the most impaired and had the fastest recovery rate. Subtle cognitive deficits in Inferential Reading Comprehension were detected when Reading Vocabulary was at or better than a twelfth grade level. Maximal recovery of all three reading functions occurred within 4 months after trauma, with most occurring in the first 3 months. The reading recovery pattern parallels the recovery of intelligence scores in the literature.

Adolescent↗
PubMed2000

Alterations of preliminary readings on radiographic examinations minimally affect outcomes of trauma patients discharged from the emergency department.

BACKGROUND: We hypothesized that trauma patients could be discharged safely from the emergency department (ED) before the availability of official readings for their radiologic examinations. We also sought to determine whether trauma patients were more prone to alterations of preliminary interpretations than other ED patients. METHODS: Alterations of preliminary readings (PR) for patients discharged from the ED were reviewed. If the official readings conflicted with the PR used for the patient's disposition, attempts were made to contact the patient and provide the appropriate follow-up. Data recorded included the type of radiographic examination, the presence of a missed injury, and the follow-up. By using institutional data, the incidence of inaccurate PR were compared for trauma patients and other ED patients (chi2 test, Fisher exact test, p < 0.05). RESULTS: Between January of 1998 and December of 1998, 102 of 38,260 discharged ED patients had official readings differing from PR. Forty-three of the changed readings involved 42 of the 1,073 discharged trauma patients, who were more likely to harbor inaccurate PR (<0.0001) than other discharged ED patients. Twenty-eight altered readings involved plain films and 15 involved computed tomographic scans. The most common altered readings involved computed tomographic scans of the head and face (n = 13). Twelve missed injuries were detected, most commonly related to a missed injury of the extremity (7 cases). Nine other cases involved the detection of incidental pathologic conditions. Eight patients required repeat ED visits for clinical and radiographic evaluation, and one patient required subsequent hospital admission. CONCLUSION: Discharged trauma patients are more likely to harbor alterations of preliminary interpretations than other ED patients. Although the official readings for these trauma patients will occasionally reveal previously undetected pathologic conditions, the majority of such cases can be managed with outpatient follow-up.

Diagnostic Errors↗
PubMed2001

Is there an association between functional vision and learning to read?

Background: Controversy exists about the role of visual parameters and vision in learning to read. This study aims to determine whether ocular parameters or performance on a dynamic test of visual function differs for children of differing reading ability. Methods: Two hundred and eighty-four children (mean age 9.9 +/- 1.8 years) received a vision screening emphasising binocular anomalies associated with discomfort at near (distance and near visual acuity, distance vision challenged with binocular +1 D lenses, near heterophoria, near point of convergence, stereopsis and accommodative facility). Non-verbal mentation age and reading accuracy were assessed. One hundred and six children performed a computerised task of motion coherence detection. Children were classified as normal readers (n = 195), children with dyslexia (n = 49) or learning disabled children (n = 40) based on their mentation age and their reading age. Results: There were no statistically significant differences or correlations between visual parameters and reading performance. Over thirty per cent of the children had accommodative facilities below or equal to six cycles per minute. Children with learning disabilities performed worst on the motion coherence task but this was statistically significant only when compared to the performance of dyslexics. Discussion: The lack of association between ophthalmic parameters and poor reading ability supports the view of the Committee on Children with Disabilities. However, 39 per cent of the children might be expected to experience difficulty 'reading to learn', as suggested by the American Academy of Optometry, as they showed anomalies associated with visual discomfort with prolonged reading. The motion coherence test did not differentiate dyslexics from normal readers and was worst in children with learning disability. Accommodative facility testing remained the most useful predictor of potential visual discomfort.

Journal Article↗
PubMed1984

Prediction of reading disability in twin boys.

Thirty-six twin boys aged between nine and 13 were assessed for intelligence and reading disability. Records of their birth status, early language development and social demographic characteristics were also available. 72 per cent of the sample had an accuracy reading age below their chronological age, and 59 per cent had a comprehension reading age below their chronological age, despite above-normal IQ. The best predictor of reading disability was preschool language status, accounting for 33 per cent of the variance in accuracy reading age discrepancy, and 23 per cent in comprehension reading age discrepancy in regression equations. This study shows that male twins are at very high risk for reading disability, and also offers support for the claim that language disability is basic to the development of reading problems.

Achievement↗
PubMed1987

Phonological similarity effects, memory span and developmental reading disorders: the nature of the relationship.

Eight-and 11-year-old poor readers of average and below average intelligence carried out a visually presented immediate memory task, recalling strings of similar- and dissimilar-sounding letters. Task difficulty was adjusted for each child by determining memory span for the dissimilar items prior to the memory task. All poor reader groups recalled significantly more dissimilar- than similar-sounding items, and the magnitude of these effects was comparable to those shown by their chronological and reading age controls. A close association was found between memory span and reading age, such that average intelligence poor readers had memory spans indistinguishable from those of their reading age controls. Furthermore, a highly significant correlation was found between memory span and reading age. However, it was concluded that this relationship could not be primarily due to a substantial immediate memory component in word recognition as the below average intelligence poor readers had poorer memory spans than the brighter poor readers, yet they had very similar reading ages. Additionally, a weaker association was found between memory span and non-word reading than between memory span and word reading. It was hypothesized that recognition difficulties may be the primary problem underlying both poor word recognition and immediate memory impairments in children with reading disorders.

Child↗
PubMed2003

Use of Read codes in diabetes management in a south London primary care group: implications for establishing disease registers.

OBJECTIVE: To establish current practice in the use of Read codes for diabetes. DESIGN: Cross sectional study. SETTING: 17 practices in the Battersea primary care group in southwest London. DATA SOURCES: Computerised medical records. MAIN OUTCOME MEASURES: Number of codes in use in all practices; variation in the use of codes between practices; and prevalence of Read code use in diabetic patients. RESULTS: At least 9 separate Read code groupings and 25 individual diabetes codes were in use in the 17 general practices. Only one Read code (C10, diabetes mellitus) and its subcodes was being used in all 17 practices, but its use varied from 14% to 98% of patients with diabetes. The use of other key Read codes for monitoring the care of patients with diabetes also varied widely between practices; for example, < 20% of practices used the code for the location of care. Less than half of patients (45%) with diabetes had their type of diabetes coded, and even fewer (21%) had measures such as the examination of the retina coded. CONCLUSIONS: The use of Read codes for diabetes needs to be standardised and coding levels improved if valid diabetic registers are to be constructed and the quality of care is to be monitored effectively. Until all patients with diabetes have the C10 Read code recorded, clinicians will have to use a wide range of Read codes and prescribing data to ensure that diabetes registers are complete.

Cross-Sectional Studies↗