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At least 649 records · Page 36Linked to original sources
PubMed1998

Ligand-induced domain movement in an antibody Fab: molecular dynamics studies confirm the unique domain movement observed experimentally for Fab NC6.8 upon complexation and reveal its segmental flexibility.

Two molecular dynamics simulations were carried out for the antibody Fab NC6.8, both with and without the guanidinium sweetener ligand NC174, in order to assess the segmental flexibility as well as the conformational changes upon ligand binding. Trajectory analyses of the simulation of the uncomplexed Fab suggest low-amplitude motions of the Ig domains with respect to each other, most clearly reflected by a periodic alteration of the elbow angle within a range of 11 degrees. Upon insertion of the hapten into the binding site, the quaternary structure of the Fab exhibits considerable rearrangements: the elbow angle changes by almost 30 degrees, the light chain is elongated and the heavy chain becomes more flexed. Comparison with experiment reveals some interesting agreements with X-ray crystallographic results published previously.

Acetates↗
PubMed2000

[Cytokine-induced nitric oxide production of joint cartilage cells in continuous passive movement. Anti-inflammatory effect of continuous passive movement on chondrocytes: in vitro study].

PURPOSE: The temporomandibular joint is a place of motion where release of proinflammatory cytokines like interleukin-1 beta (IL-1 beta) induces cartilage destruction via production of nitric oxide (NO). The purpose of this study was to evaluate the effects of continuous passive motion in the form of cyclic stretch on the synthesis of inducible nitric oxide synthase (iNOS). METHODS: Articular chondrocytes were harvested from rabbit cartilage slices and cultured in F12 medium supplemented with 10% fetal calf serum. Subsequently, cells (10(5)/ml per well) were transferred to Flexercell plates, grown to 90% confluency, and subjected to one of the following regimens: (1) no mechanical strain (10 Hz, 20% elongation rate); (2) rhIL-1 beta (1 ng/ml); (3) mechanical strain; (4) mechanical strain and rhIL-1 beta. The cells were exposed to cyclic stretch for 24 h. Thereafter, cells were trypsinized and centrifuged on microscope slides in a cytospin centrifuge. The presence of iNOS was determined by indirect immunoperoxidase staining using polyclonal rabbit anti-iNOS as primary antibodies, and goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) as secondary antibodies. Diaminobenzidine was used as substrate for HRP. Total NO production was spectrophotometrically measured in the supernatants of cultures using Greiss reaction. All experiments were done in triplicates and the statistical significance was analyzed by Student's t-test. RESULTS: Cells with treatment 1 and 3 did not exhibit presence of iNOS. IL-1 beta-treated cells in group 2 exhibited intense peroxidase staining. Cells in group 4 exposed to IL-1 and mechanical stress showed staining with considerably less intensity. Control cells treated with normal rabbit IgG as a primary antibody did not exhibit peroxidase staining. The data were further confirmed by measurement of statistically significant differences of NO levels in supernatants assessed in the four different treatment groups. CONCLUSION: Our findings suggest that continuous passive motion exerts anti-inflammatory effects on chondrocytes, downregulating the quantity of iNOS-positive cells. Since NO acts as an intracellular, transcellular, and cytotoxic molecule, it has a basic importance for proper post-traumatic and postoperative TMJ function as well as the course and therapy of inflammatory TMJ diseases.

Animals↗
PubMed1991

[Complex movement analysis: dynamic study of the effect of a tape bandage on pronation in high-jumping with three-dimensional movement analysis and electromyography].

Overuse syndromes in the lower extremity are a frequent problem in athletics, especially in jumping events. Athletes notice a comfortable support by using ankle taping without restriction of their performance. Up to now no objective data is available about efficiency of taping against overpronation which is frequent in high jumping. In high jumping without boots little difference of pronation between taped and untaped take off leg was found. In contrast in jumping with spikes boots a marked pronation during take off was found, which was reduced by ankle taping with a reduction of maximum medial malleolar medialisation by 77%, reduction of pronation time by 33% and time of maximum pronation by 8.2% in the take off leg. Surface electromyography showed an increased activity of M. peroneus longus by 30.7% as a plantarflector and pronator muscle with ankle taping. Activity of other jump supporting muscles of the lower extremity was little influenced.

Adolescent↗
PubMed2005

Random and blocked practice of movement sequences: differential effects on response structure and movement speed.

Three similar six-element key press sequences were practiced under blocked or random practice schedules with acquisition conducted on one day and retention and transfer on the next day. The task required participants to type, as quickly as possible, one of three 6-element sequences as observed on a computer monitor. In blocked practice, participants completed all practice in one repeated sequence before the next repeated sequence was introduced. In random acquisition practice, the three repeated sequences were randomly presented to the participants. The data suggest that random practice results in participants adopting a uniform response structure, while blocked practice allows participants to exploit unique sequential aspects of the individual tasks. This finding suggests that random practice may not be as effective as blocked practice when one of the tasks being practiced together can be optimized through the development of a unique response structure.

Humans↗