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At least 649 records · Page 36Linked to original sources
PubMed2003

Nutrient content of some mushroom species of the genus Termitomyces consumed in Cameroon.

The nutrient content (water, lipids, proteins, ashes, crude fibres) of six mushroom species (Termitomyces le Testui, T. aurantiacus, T. schimperi, T. mammformis, T. mboudaeïna Mossebo sp. nov., and T. subcypeatus forme bisporus sp. nov.) of the genus Termitomyces from Cameroon was determined. These mushrooms have a high water content (83.3-94.3 g/100 g wet matter) and contain more lipids than species from temperate countries (2.5-5.4 g/100 g dry weight), with high proportions of polyunsaturated fatty acids (45.1-65.1% of total fatty acid methyl esters) and remarkable proportions of crude fibres (17.5-24.7 g/ 100 g dry weight). Their protein content varied between 15.1 and19.1 g/100 g dry weight and ash content between 5.2 and 14.4. The species T. mammiformis was found particularly rich in minerals with 14.4 g ash/ 100 g dry material, that is up to 2.4 g/100 g fresh weight.

Agaricales↗
PubMed2006

Fungicide control of mushroom cobweb disease caused by Cladobotryum strains with different benzimidazole resistance profiles.

The benzimidazole fungicides thiabendazole and carbendazim, and the imidazole fungicide prochloraz-Mn, were tested for their efficacy in controlling cobweb disease of mushrooms caused by two Cladobotryum isolates. Isolate 202A was benzimidazole-sensitive in vitro and cobweb growth on the casing was well controlled by both benzimidazole fungicides in cropping experiments. Carbendazim also controlled the development of spotting symptoms much more effectively than thiabendazole. A second isolate (192B1) was benzimidazole-resistant and was highly resistant to thiabendazole in vitro but it showed some sensitivity to carbendazim in vitro at moderate to high concentrations. Despite this, carbendazim did not control disease symptoms in cropping experiments, confirming that isolate 192B1 is cross-resistant to other benzimidazole fungicides. Both isolates showed some sensitivity to prochloraz-Mn in vitro. This fungicide gave between 45% and 65% control of cobweb growth on the casing caused by either 202A or 192B1 but gave no control of spotting symptoms. Reducing the fungicide application volume did not give enhanced disease control. The emergence of benzimidazole resistance reduces the value of benzimidazoles in the control of mushroom pathogens. However, the lack of effective alternatives means they continue to have utility in cases where pathogens are still sensitive but this requires regular monitoring of pathogen populations for resistance.

Agaricus↗
PubMed2004

Methanol extract of the oyster mushroom, Pleurotus florida, inhibits inflammation and platelet aggregation.

The antiinflammatory and platelet aggregation inhibiting activities of the methanol extract of Pleurotus florida Eger, an edible and commercially grown mushroom, were investigated. The extract showed significant activity in ameliorating acute inflammation induced by carrageenan and chronic inflammation by formalin at 500 and 1000 mg/kg body weight. The effect was comparable to the standard reference drug, diclofenac. The extract also showed significant platelet aggregation inhibiting activity of washed human platelets. Adenosine 5'-diphosphate (ADP) induced platelet aggregation was inhibited by 88% to 95% at a concentration of 500 microg/mL of the extract after a preincubation time of 5, 10 and 20 min. The marked antiinflammatory and platelet aggregation inhibiting properties of this mushroom suggest its potential therapeutic use against vascular disorders.

Adenosine Diphosphate↗
PubMed1998

A mushroom fruiting body-inducing substance inhibits activities of replicative DNA polymerases.

We found and isolated two natural products in the extract from a basidiomycete, Ganoderma lucidum, as eukaryotic DNA polymerase inhibitors. The compounds were identified as cerebrosides, (4E,8E)-N-D-2'-hydroxypalmitoyl- 1-O-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine and (4E,8E)-N-D-2'-hydroxystearoyl-1-O-beta-D-glucopyranos yl-9-methyl- 4,8-sphingadienine and were found to be identical to the mushroom fruiting body-inducing substances (FIS) reported. These cerebrosides selectively inhibited the activities of replicative DNA polymerases, especially the alpha-type, from phylogenetically broad eukaryotic species, whereas they hardly influenced the activities of DNA polymerase beta, prokaryotic DNA polymerases, terminal deoxynucleotidyl transferase, HIV reverse transcriptase, RNA polymerase, deoxyribonuclease I, and ATPase. The inhibition of another replicative polymerase, the delta-type, was moderate. The inhibitions of the replicative polymerases were dose-dependent, and the IC50 for animal or mushroom DNA polymerase alpha was achieved at approximately 12 micrograms/ml (16.2 microM) and for animal DNA polymerase delta at 57 micrograms/ml (77.2 microM). FIS is possibly a DNA polymerase inhibitor specific to the replicative enzyme group, and the fruiting body formation may be required for the suppression of the DNA replication or the vegetative growth of the mycelium.

Animals↗
PubMed2001

Pleureryn, a novel protease from fresh fruiting bodies of the edible mushroom Pleurotus eryngii.

A protease designated pleureryn, with an N-terminal sequence dissimilar from previously reported mushroom metalloendopeptidases and showing only limited resemblance to aspartic proteinases, albeit considerable homology to DNA replication licensing factor, was isolated from fresh fruiting bodies of the edible mushroom Pleurotus eryngii. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel Blue gel, ion exchange chromatography on CM-Sepharose, and FPLC-gel filtration on Superdex 75. The protease was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel Blue gel and CM-Sepharose. It demonstrated a single band with a molecular weight of 11.5 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Pleureryn demonstrated a protease activity of 9364 U/mg toward casein. It exhibited a pH optimum of 5.0 and a temperature optimum of 45 degrees C, with substantial activity remaining at high temperatures and pH 4 and 12. The activity of the protease was adversely affected by pepstatin A, indicating that it is an aspartic protease. PMSF, trypsin inhibitor, and EDTA exerted no striking effect, suggesting that it is neither a serine protease nor a metalloprotease. It inhibited translation in a rabbit reticulocyte lysate system with an IC(50) of 20 nM. Pleureryn also exhibited some inhibitory activity against HIV-1 reverse transcriptase, reminiscent of a suppressive action of HIV-1 protease on its homologous reverse transcriptase but was devoid of ribonuclease, deoxyribonuclease, and antifungal activities.

Amino Acid Sequence↗
PubMed2000

Molecular cloning of a widely distributed microsatellite core sequence from the cultivated mushroom Agaricus bisporus.

An Agaricus bisporus microsatellite with the tetranucleotide motif TATG tandemly repeated was isolated from an A. bisporus library enriched in repeated sequences. The use of the 16-mer oligonucleotide (TATG)4 indicates that many loci contain nearby copies of the microsatellite in opposite orientations. The wide distribution of the microsatellite in the A. bisporus genome was assessed (i) by polyacrylamide gel electrophoresis of the products generated by directed amplification of microsatellite-region DNA (DAMD) and (ii) by hybridization of these products with A. bisporus chromosomes separated by pulsed-field gel electrophoresis. This is, to our knowledge, the first microsatellite reported in the cultivated edible mushrooms. DAMD-PCR products were generated using DNA of three Pleurotus species (P. pulmonarius, P. sajor-caju, and P. florida), indicating that (TATG)4 repeats are also present in these cultivated species. The variability found within closely related strains indicates that such microsatellites are useful in fingerprinting and studying genetic variability in wild and commercial mushrooms.

Agaricus↗
PubMed1999

Phylogenetic relationships in the mushroom genus Coprinus and dark-spored allies based on sequence data from the nuclear gene coding for the large ribosomal subunit RNA: divergent domains, outgroups, and monophyly.

Phylogenetic relationships were investigated in the mushroom genus Coprinus based on sequence data from the nuclear encoded large-subunit rDNA gene. Forty-seven species of Coprinus and 19 additional species from the families Coprinaceae, Strophariaceae, Bolbitiaceae, Agaricaceae, Podaxaceae, and Montagneaceae were studied. A total of 1360 sites was sequenced across seven divergent domains and intervening sequences. A total of 302 phylogenetically informative characters was found. Ninety-eight percent of the average divergence between taxa was located within the divergent domains, with domains D2 and D8 being most divergent and domains D7 and D10 the least divergent. An empirical test of phylogenetic signal among divergent domains also showed that domains D2 and D3 had the lowest levels of homoplasy. Two equally most parsimonious trees were resolved using Wagner parsimony. A character-state weighted analysis produced 12 equally most parsimonious trees similar to those generated by Wagner parsimony. Phylogenetic analyses employing topological constraints suggest that none of the major taxonomic systems proposed for subgeneric classification is able to completely reflect phylogenetic relationships in Coprinus. A strict consensus integration of the two Wagner trees demonstrates the problematic nature of choosing outgroups within dark-spored mushrooms. The genus Coprinus is found to be polyphyletic and is separated into three distinct clades. Most Coprinus taxa belong to the first two clades, which together form a larger monophyletic group with Lacrymaria and Psathyrella in basal positions. A third clade contains members of Coprinus section Comati as well as the genus Leucocoprinus, Podaxis pistillaris, Montagnea arenaria, and Agaricus pocillator. This third clade is separated from the other species of Coprinus by members of the families Strophariaceae and Bolbitiaceae and the genus Panaeolus.

Coprinus↗
PubMed1994

The nucleotide sequence and genome organization of mushroom bacilliform virus: a single-stranded RNA virus of Agaricus bisporus (Lange) Imbach.

Mushroom bacilliform virus (MBV) is found in association with spherical virus-like particles in cultivated mushrooms (Agaricus bisporus) afflicted with La France disease. MBV possesses a monopartite ssRNA genome of positive sense and differs from the majority of characterized mycoviruses, which contain segmented dsRNA genomes. We have cloned and sequenced the MBV genome and determined that its length is 4009 nucleotides. The MBV genome contains four major and three minor open reading frames and has 5' and 3' noncoding regions of 60 and 250 nucleotides, respectively. The putative RNA-dependent RNA polymerase and the coat protein display homology with corresponding proteins encoded by certain plant viruses, particularly luteoviruses and carmoviruses.

Agaricus↗
PubMed1998

Mushroom bacilliform virus RNA: the initiation of translation at the 5' end of the genome and identification of the VPg.

Mushroom bacilliform virus (MBV) is often found in cultivated mushrooms (Agaricus bisporus) with La France disease. MBV has a 4-kb ssRNA genome of positive-sense encoding four major open reading frames (ORFs). The arrangement of ORFs at the 5' end of the genome and the deduced amino-acid sequences of two of the putative gene products (protease and RNA-dependent RNA polymerase) show remarkable similarity to some plant viruses, particularly subgroup II luteoviruses. We show that this similarity extends to the translation strategy at the 5' end of the genome, the presence of a genome-linked protein (VPg), and the location of the VPg downstream of the protease motifs in the polypeptide encoded by ORF2.

Agaricus↗
PubMed1999

Identification of a subgenomic mRNA encoding the capsid protein of mushroom bacilliform virus, a single-stranded RNA mycovirus.

Mushroom bacilliform virus is unique among mycoviruses of the higher fungi in having a genome of positive-sense single-stranded RNA. We have identified a subgenomic mRNA molecule encoding the viral capsid protein in mushroom bacilliform virus-infected mycelium of Agaricus bisporus. Transcription of subgenomic RNA commences at the sequence ACAAAA, 47 nucleotides upstream of the initiating AUG.

Agaricus↗
PubMed1995

The mushroom-inducing gene Frt1 of Schizophyllum commune encodes a putative nucleotide-binding protein.

Fruiting bodies (mushrooms) can be induced to form in unmated, normally non-fruiting strains of the basidiomycete fungus Schizophyllum commune by the ectopic genomic integration of a cloned gene called Frt1. Thus, the normal requirement of mating for mushroom formation is bypassed. Sequence analysis of genomic and cDNA clones revealed that the Frt1 gene encodes a predicted polypeptide of 192 amino acids, interrupted by three short introns. The FRT1 protein is predicted to be of M(r) 21,625 and does not have significant overall similarity to any known proteins. Analysis of the predicted amino acid sequence revealed the presence of a P-loop motif, a conserved sequence found in nucleotide-binding proteins. A potential site for Mg2+ binding is predicted to reside next to the P-loop at Thr24. The possible functional significance of these and other residues within FRT1 was examined using site-directed mutagenesis, followed by transformation of these mutant alleles of Frt1 back into S. commune. Mutation of the middle glycine of the P-loop completely abolished the fruit-inducing activity of cloned Frt1. Substitution of an alanine residue for Thr24 also resulted in mutant clones with no fruit-inducing activity. The possibility of an interaction between two closely spaced threonine residues within FRT1 was suggested by transformation experiments utilizing mutant Frt1 alleles with specific combinations of mutations at these sites. Taken together, the results of our mutagenesis experiments suggest the possibility that activity of the predicted FRT1 protein could be altered by nucleotide binding and coordination of Mg2+. Northern blot hybridization experiments indicate that Frt1 activity is probably not controlled at the transcriptional level.

Alanine↗
PubMed1991

Sequences of three dsRNAs associated with La France disease of the cultivated mushroom (Agaricus bisporus).

La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins with molecular weights of 38, 40 and 87 kDa were found for M2, M1 and L3 dsRNAs, respectively. The average G + C content of these dsRNAs was 43%, close to that of A. bisporus nuclear DNA. The nucleotide sequences, as well as the amino acid sequences, appear to be unique, as no matching sequences could be found among databases. S3 dsRNA (0.39 kb), which is occasionally found in large amounts in diseased mushrooms, is an internally deleted variant of M2 dsRNA and is largely composed of the non-coding ends of that dsRNA.

Agaricus↗
PubMed1988

Toxic properties of the mushroom Cortinarius orellanus. I. Chemical characterization of the main toxin of Cortinarius orellanus (Fries) and Cortinarius speciosissimus (Kühn & Romagn) and acute toxicity in mice.

The toxins of the mushrooms Cortinarius orellanus (Fries) and Cortinarius speciosissimus (Kühn & Romagn) were isolated by extraction procedures and Sephadex chromatography. All intermediate and end products of the purification process were tested in mice for acute toxicity after oral and i.p. administration. In both species a fluorescent main toxin and a nonfluorescent compound of minor toxicity were found. The main toxin of both species was identified by mass spectrometric and nuclear magnetic resonance analyses as the 2,2'-bipyridine-3,3',4,4'-tetrol-1,1'-dioxide, which is identical to orellanine. The purified compound was toxic when administered either orally or i.p. When given orally the LD50 was 33 mg/kg body weight in mice. The oral LD50 of Cortinarius orellanus (2.20 g dried mushroom/kg) and of Cortinarius speciosissimus (3.12 g/kg) depended on the orellanine content (14 mg/g in Cortinarius orellanus and 9 mg/g in Cortinarius speciosissimus). The second toxic component was ineffective in mice when given orally. It caused acute toxicity when administered i.p., but toxicity was low when compared to the main toxin. Thus it appears to be of minor importance. Intraperitoneal testing of both isolated toxins and of the extract containing the whole toxic potential of the mushrooms revealed that toxicity, time dependency and expression of toxicosis is accounted for by the sum of these two toxins. No peptidic main toxin as described by other mycologists could be detected.

2,2'-Dipyridyl↗
PubMed1988

Toxic properties of the mushroom Cortinarius orellanus (Fries). II. Impairment of renal function in rats.

Oral administration of the toxic mushroom Cortinarius orellanus (Fr.) to male Sprague Dawley rats caused serious impairment of renal function. The signs observed were similar to those produced in humans who ingest this fungus. Administration of 2.0 g dried Cortinarius orellanus per kg body weight led to acute renal dysfunction within 48 h. The pattern of impairment included reduced glomerular filtration rate, decreased renal absorption of water, sodium and potassium, and proteinuria and glucosuria. The nephrotoxic effect was further characterized by decreased activities of the brush border enzymes alkaline phosphatase and gamma-glutamyltranspeptidase in urine, despite a remarkable increase in protein excretion of predominantly tubular origin. These findings were substantiated by morphologic changes, which could be detected as early as 12 h after dosing. Morphologically discernible signs of renal tubular damage start with deformation of the proximal tubular brush border region. Within 48 h after toxin ingestion, prenecrotic and necrotic cells could be found in all nephron segments contained in the renal cortex. The most prominent changes were a vesiculation of the apical cell pole and a swelling of the smooth surfaced endoplasmic reticulum and of mitochondria. The latter was accompanied by a loss in matrix material and a massive fragmentation of mitochondrial cristae membranes. Detectable quantities of the toxic principle of the mushroom, orellanine, were excreted only within the first 24 h after dosing. No impairment of liver function was detected.

Agaricales↗
PubMed1988

Nephrotoxicity of orellanine, a toxin from the mushroom Cortinarius orellanus.

Pure orellanine extracted from the mushroom Cortinarius orellanus is highly toxic in mice both when given intraperitoneally (LD50 = 12.5 mg/kg) or per os (LD50 = 90 mg/kg). The kidneys of mice injected i.p. with orellanine show similar tubular necrosis to that obtained with whole mushroom given per os. This demonstrates that orellanine is the true, principal toxin of C. orellanus. The similarity of the structures of the toxin and of bipyridinium herbicides such as methylviologen (paraquat) or the nephrotoxic diquat led other authors to presume a similar mechanism for orellanine toxicity. Our study of the electrochemical behaviour of orellanine shows that its mechanism of action has to be different from that of these poisons.

2,2'-Dipyridyl↗
PubMed1980

[Fecal excretion of cadmium and copper after mushroom (Agaricus) diet (author's transl)].

High contents of cadmium in some agaricus species led to the warning that the eating of wild-grown mushrooms may bear the possibility of cadmium-intoxication. The low digestion rate due to the chitin membrane of fungi was not discussed. Therefore, in this investigation the cadmium- and copper-concentrations in feces of five subjects were estimated before and after a three days mushrooms diet. The high amount of fecal cadmium and copper increasing after the diet confirm the suggestion, that eaten fungi mostly pass through the intestinal tract unscathed without resorption. By this even larger ingestions of agaricus fungi may not cause cadmium intoxication in humans.

Basidiomycota↗
PubMed1978

[The potassium content of mushrooms (author's transl)].

The potassium content of 410 species (1075 samples) of wild mushrooms ranged between 1.5 and 117g/kg dry wt. It was dependent on species and genus, and on the average was highest in Coprinaceae and lowest in Polyporaceae. A positive correlation was observed between potassium and water content; consequently, the potassium content of the wet wt. showed less variation: it ranged between 0.6 and 14.6 g/kg. In the solid matter of single fruit-bodies (11 species) potassium was distributed as follows: flesh of the cap greater than stem greater than gills or tubes greater than spores. The flesh of the cap contained maximally 1.8 times the potassium of the gills. But due to a correspondingly higher water content, significant potassium differences between fresh weights of these parts of fruit-bodies were uncommon. Potassium was concentrated 20--40-fold in fruit-bodies as compared with the soil.--According to our results mushrooms rank among the most potassium-rich vegetables, appropriate for consideration in diets in chronic potassium deficiency. On the other hand, toxic effects must be considered, especially in cases of renal insufficiency.

Basidiomycota↗
PubMed1978

[Cadmium in mushrooms (author's transl)].

The cadmium content of 402 species of wild mushrooms was determined by flameless atomic absorption spectroscopy. Altogether 1049 samples, grown mainly, in southern Germany, were tested. The cadmium content was between less than 0,1 and 120 mg/kg dry weight, equivalent to less than 0,01 and 10,8 mg/kg fresh weight. Samples with low cadmium content were predominant: 68% of the samples contained less than 2 mg/kg dry weight, 86.5% contained less than 5 mg/kg dry weight, equivalent to about less than 0,2 and less than 0,5 mg/kg fresh weight, respectively. The cadmium content was clearly species-dependent, and to a lesser extent genus-dependent. Samples containing more than 10 mg/kg dry weight occurred in 41 species, among these were 9 Tricholomataceae, 10 Agaricaceae, 11 Cortinariaceae, 3 Amanitas and 4 Russula species. Samples containing more than 50 mg/kg dry weight were found in Agaricus augustus, A perrarus, A. silvicola, A. macrosporus, A. maleolens, and Inocybe bongardii. In single fruiting-bodies the lowest cadmium content was found in the stem, whereas the highest content was found in the gills and tubes. Cadmium content of the gills was at most five times the amount present in the cup. In cadmium-rich mushrooms a marked concentration as compared with the cadmium content of the soil had occured.

Basidiomycota↗