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PubMed2000

Bacterial artificial chromosome libraries for mouse sequencing and functional analysis.

Bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) libraries providing a combined 33-fold representation of the murine genome have been constructed using two different restriction enzymes for genomic digestion. A large-insert PAC library was prepared from the 129S6/SvEvTac strain in a bacterial/mammalian shuttle vector to facilitate functional gene studies. For genome mapping and sequencing, we prepared BAC libraries from the 129S6/SvEvTac and the C57BL/6J strains. The average insert sizes for the three libraries range between 130 kb and 200 kb. Based on the numbers of clones and the observed average insert sizes, we estimate each library to have slightly in excess of 10-fold genome representation. The average number of clones found after hybridization screening with 28 probes was in the range of 9-14 clones per marker. To explore the fidelity of the genomic representation in the three libraries, we analyzed three contigs, each established after screening with a single unique marker. New markers were established from the end sequences and screened against all the contig members to determine if any of the BACs and PACs are chimeric or rearranged. Only one chimeric clone and six potential deletions have been observed after extensive analysis of 113 PAC and BAC clones. Seventy-one of the 113 clones were conclusively nonchimeric because both end markers or sequences were mapped to the other confirmed contig members. We could not exclude chimerism for the remaining 41 clones because one or both of the insert termini did not contain unique sequence to design markers. The low rate of chimerism, approximately 1%, and the low level of detected rearrangements support the anticipated usefulness of the BAC libraries for genome research.

Animals↗

PubMed1975

From poor farm to medical center: a century of library services to the Los Angeles County/University of Southern California Medical Center.

As its centennial approaches, the history and development of library services to the patients and professional staff of the Los Angeles County/University of Southern California Medical Center is traced from the early days when the library was housed in the cafeteria of the dispensary to its present position of being first point of access to library service for one of the largest teaching hospitals in the country. Its recent affiliation with the Norris Medical Library of the University of Southern California School of Medicine is explained. The change in emphasis from patients' library to health sciences library is illustrated, and the contribution of the library to the Cumulative Index to Nursing Literature is detailed.

California↗

PubMed2000

Construction and analysis of cDNA library of Necator americanus third stage larvae.

OBJECTIVE: To obtain the genetic information on Necator americanus and to search for the purpose genes. METHODS: mRNA was isolated from the third stage larvae of Necator americanus maintained in hamsters. Double strand cDNA was synthesized and ligated to lambda ZAPII vector to construct the cDNA library. Expressed sequence tages (ESTs) were obtained by single pass sequencing of randomly isolated cDNA clones from the established library. RESULTS: A cDNA library of N. americanus was successfully constructed with high recombinant efficiency. The titer of unamplified library was 1 x 10(7). The insert size was about 750-3,000 bp. Of 11 ESTs obtained from the library, 7 have a significant homology with certain functional genes. CONCLUSION: A high quality and high representative cDNA library of N. americanus was constructed at the first time and some functional genes were identified from the library by ESTs.

Animals↗

PubMed1999

[Construction of a pufferfish gridded cosmid library].

OBJECTIVE: Pufferfish is a newly-established model organism in human genome research, which has been demonstrated its unique advantages in comparative genomics. Construction of a genomic library of pufferfish is the prerequisite to any studies of its genome. METHODS: Genomic DNA from Fugu rubripes, a species distribute only in Japan and China seas, was used to construct a library in a novel cosmid vector, named sCOGH2, which contains an exon-trapping cassette. RESULTS: This library is composed of 57,600 clones gridded in 60,096-well cell culture plates, one clone each well. The average size of the inserts is around 35 kb. It provides 99.2% probability to find any unique DNA fragment of pufferfish in this library. The clones of this library can survive after more than 10 times freeze-thaw, also demonstrating high stability in propagation. The clones shone highly positive signals when hybridized with the pufferfish genomic DNA. CONCLUSIONS: The library accords with qualitative demands of a cosmid library.

Animals↗

PubMed2003

The Association of Academic Health Sciences Libraries Annual Statistics: a thematic history.

The Annual Statistics of Medical School Libraries in the United States and Canada (Annual Statistics) is the most recognizable achievement of the Association of Academic Health Sciences Libraries in its history to date. This article gives a thematic history of the Annual Statistics, emphasizing the leadership role of editors and Editorial Boards, the need for cooperation and membership support to produce comparable data useful for everyday management of academic medical center libraries and the use of technology as a tool for data gathering and publication. The Annual Statistics' origin is recalled, and survey features and content are related to the overall themes. The success of the Annual Statistics is evident in the leadership skills of the first editor, Richard Lyders, executive director of the Houston Academy of Medicine-Texas Medical Center Library. The history shows the development of a survey instrument that strives to produce reliable and valid data for a diverse group of libraries while reflecting the many complex changes in the library environment. The future of the Annual Statistics is assured by the anticipated changes facing academic health sciences libraries, namely the need to reflect the transition from a physical environment to an electronic operation.

Annual Reports as Topic↗

PubMed2005

[Construction of a large human scFv library against SARS virus].

AIM: To construct a large human scFv library against SARS virus by using in vivo recombination. METHODS: Total RNA was isolated from the lymphocytes of 6 patients recovered from SARS. mRNA was isolated and reverse transcribed into cDNA. The V(H) and V(L) fragments were amplified from the cDNA and then assembled into scFv genes. The scFv genes were amplified and ligated into phagemid pDAN5. The primary library was constructed by transforming the recombinant phagemid into E.coli TG1. The secondary library was generated by in vivo recombination in E.coli BS1365 following the infection of BS1365 by primary library phages. RESULTS: A primary library of 3x10(9) and a second library of 3x10(11) were constructed. CONCLUSION: A large human scFv library against SARS virus with good diversity was constructed, which may be used for screening antibodies to SARS virus antigens.

Antibodies, Viral↗

PubMed1991

Cloning and direct sequencing from lambda cDNA libraries using the polymerase chain reaction: suppressin and the vasopressin receptor as models.

A strategy using the polymerase chain reaction (PCR) to screen a lambda gt11 pituitary cDNA library for cDNAs encoding suppressin, a putative anti-proliferative protein, and a putative vasopressin receptor is described. The use of this technique will facilitate the demonstration of e.g. the presence of "neuropeptide receptors" on cells of the lymphoid system, confirming the concept of "shared ligands and receptors" by the neuroendocrine and the immune system. Neither of the genes encoding the proteins of the present study have previously been cloned. The PCR-screening procedure requires sequence information from the gene of interest which permits the generation of complementary primers. These primers are then used in combination with lambda phage primers complementary to regions flanking the cloning site in a PCR to amplify cDNAs derived from the gene of interest. This novel screening procedure yields cDNA related to the gene of interest, including the largest clone present in the library. To confirm the utility of this technique for cDNA libraries, the library was also screened using traditional cDNA hybridization techniques. The largest clone obtained by screening the cDNA library with PCR was the same as that obtained by the conventional technique. Thus, the results of these studies show that the PCR method can be used instead of more conventional means to screen cDNA libraries. Lastly, we describe a protocol for directly sequencing PCR-amplified DNA using the same primers that are used for amplification. The combined use of these two strategies permits cloning and sequencing of cDNAs from lambda cDNA libraries in a fraction of the time required using traditional screening techniques, but with identical results.

Animals↗

PubMed2006

[Construction of scFv antibody mini-library to 3 cellular surface molecules].

To construct a scFv antibody mini-library by phage display technique from the spleen cells of BALB/c mice immunized with extracellular domain of N-cadherin (N-cad), CD34 and AC133, the extracellular domain genes of N-cad, CD34 and AC133 were cloned into a phagemid pSEX81 respectively, and were then displayed on the phagemid in the form of fusion protein with p III protein. After being effectively immunized with the fusion protein, the spleen of the mice were harvested, and total RNA were extracted from the spleen. The cDNA of VH and VL genes were amplified by RT-PCR, and a scFv-phage display antibody library was constructed with the amplified V-genes. The content, multiplicity and expressing potential of the library were examined. As a result, we had produced a scFv library containing 1.4 x 10(6) individual clones which showed different patterns after being digested with restriction endoneuclease Mua I. The surface display expression of the library was also verified. It indicated that the capacity and diversity of the library was sufficient for screening specific scFv antibody against N-cad, CD34 and AC133. The library will be useful for isolating corresponding specific scFv antibodies.

AC133 Antigen↗

PubMed1991

Accuracy of telephone reference service in health sciences libraries.

Six factual queries were unobtrusively telephoned to fifty-one U.S. academic health sciences and hospital libraries. The majority of the queries (63.4%) were answered accurately. Referrals to another library or information source were made for 25.2% of the queries. Eleven answers (3.6%) were inaccurate, and no answer was provided for 7.8% of the queries. There was a correlation between the number of accurate answers provided and the presence of at least one staff member with a master's degree in library and information science. The correlation between employing a librarian certified by the Medical Library Association (MLA) and providing accurate answers was significant. The majority of referrals were to specific sources. If these "helpful referrals" are counted with accurate answers as correct responses, they total 76.8% of the answers. In a follow-up survey, five libraries stated that they did not provide accurate answers because they did not own an appropriate source. Staff-related problems were given as reasons for other than accurate answers by two of the libraries, while eight indicated that library policy prevented them from providing answers to the public.

Follow-Up Studies↗

PubMed1990

Journal deselection in a biomedical research library: a mediated mathematical approach.

A unique mathematical formula was developed to use for journal deselection decisions. The formula factors in subscription cost, shelving and storage cost, interlibrary loan cost, staffing cost, and use level to determine the institutional cost ratio; this ratio serves as an indicator of the cost-effectiveness of each subscription title. Once the institutional cost ratio was calculated for each of 537 titles, a committee of library staff and senior library customers reviewed the ranked list to decide which subscriptions should be canceled. The committee also considered possible exceptions based on subjective criteria such as availability at local libraries, unrecorded use, and relative importance of the journal. The preliminary cancellation list was then reviewed by the library's research users. They were able to justify library subscriptions to a few additional titles. This method enabled the library to cut its subscription costs by 46%, while cutting only 8% of the total use. In addition, by mediating the mathematical approach with human intervention, the library made these severe cuts without unduly distressing its patrons.

Book Selection↗

PubMed1990

Impact of changing health care economics on Michigan hospital libraries: report of a survey.

In an attempt to determine how efforts to contain the soaring cost of U.S. health care in the 1980s have affected the size and stature of Michigan hospital libraries, a survey was sent out in April 1988 to 156 hospitals across the state. Fifteen of the 97 responding institutions did not maintain libraries during the survey period and were subsequently excluded from the study. Total FTEs in the 82 responding libraries decreased by 6.1%. In the 69 libraries reporting budget data, materials budgets increased, but by less than 7% a year. Eighteen of the 82 libraries had positions reclassified, with 12 (14.6%) assigned downgrades. The trend toward reduced size and perceived value of hospital libraries that emerged from the survey points to the need for regular collection of comprehensive hospital library statistics to assist hospital librarians in effective direction of their profession's course.

Data Collection↗

PubMed1990

The changing continuing education role of health sciences libraries.

Libraries have always organizationally supported the continuing education (CE) objectives of their respective institutions. As CE experts increase their understanding of the learning process and the factors that make CE opportunities successful, it is important that health sciences librarians use this knowledge to enhance their positions as key players in the CE field. This paper surveys the literature related to the roles of health sciences libraries in CE, reports an informal survey of health sciences librarians, and identifies innovative services that integrate the library with the lifelong learning processes of its users. Four distinct support areas are identified in which the library relates to CE (resources, content, education, and information management), illustrating traditional library CE roles and suggesting new opportunities. To be successful in improving the library's role in CE, librarians must attend to their own lifelong learning needs, increase collaboration with educators and CE providers, participate in research that addresses the learning and information assimilation processes, and actively involve the library in the quality filtering process.

Education, Continuing↗

PubMed1986

From Surgeon General's bookshelf to National Library of Medicine: a brief history.

The National Library of Medicine originated as a few books in the office of the army's surgeon general, Joseph Lovell, between 1818 and 1836. It became the nation's largest medical library after the Civil War under the direction of John Shaw Billings and began publishing the Index-Catalogue of the Library of the Surgeon General's Office and preparing the Index Medicus. After Billings retired in 1895, the library marked time as army medical officers were rotated through as directors until modernization began under Harold Wellington Jones during World War II. during the directorship of Frank B. Rogers (1949-1963), who introduced MEDLARS, guided the move to a new building in Bethesda, and revitalized other operations, the institution received statutory authority as the National Library of Medicine within the Public Health Service (1956). By 1965, which was marked by the passage of the Medical Library Assistance Act, the library had again regained a position of world leadership.

History, 19th Century↗

PubMed1973

Review of resource grant applications at the National Library of Medicine.

The Medical Library Resource Grant Program of the National Library of Medicine is reviewed as it was administered under the Medical Library Assistance Act and as it is now administered under the Medical Library Extension Act of 1970, and program objectives are discussed. The "project" concept and the "general support" approach for library assistance are considered, as is the relationship of the Regional Medical Library Program. In the context of the evolving Biomedical Communications Network, recommendations are made for improving the effectiveness of the grant program through coordinated efforts of intermediary libraries.

Financing, Government↗

PubMed1967

Stimulating growth and development of medical society libraries.

A library can represent the most valuable personal service which a medical society can offer to a physician; yet, libraries unfortunately are taking a lessening role in medical societies. Surveys show that medical society libraries have declined in number from seventy-one in 1942 to forty-eight at the present time. This paper offers a positive plan of action with the objective of enabling libraries to regain a position of eminence in medical societies. It is pointed out that there are four vital elements in stimulating the growth and development of a library. These are the constant enhancement of resources; the promotion of library services; the rendering of prompt, valuable services; and ultimately, the recognition of the library through gifts, endowments, and expressions of appreciation.

Libraries, Medical↗

PubMed1984

Academic health sciences libraries, then and now.

We have made no attempt to compare academic health science libraries along other criteria. Collections and services are not always congruent with budgets; nor do we imply that bigger is better. It is much more expensive to operate a library today than in 1962. In addition, building backfiles is not usually required for a library that has been collecting, even modestly, for fifty to one hundred years. In summary, the eastern seaboard health science libraries generally declined in rank, whereas those in the midwest, southwest, and west either remained in rank order or increased in rank. Exceptions are in North and South Carolina. Although this socioeconomic shift is interesting, the more relevant finding is the narrowing range. In 1962 the median health science library budget was one-sixth as large as that of top-ranked UC-SF. Whereas UC-SF's budget has shown a six-fold increase over twenty years, the median library's budget has shown a twelve-fold increase. The gap between the annual budgets of the top-ranked schools and those in the bottom half of the range remains substantial but is apparently closing. It is anticipated that during the next two decades relatively few dollars will separate the annual budgets of the top ten or twenty academic health science libraries from those at the other end of the spectrum.

Budgets↗

PubMed1983

The library and its home computer: automation as if people mattered.

To provide its users with quick and easy access to the library resources, the Muriel and Philip Berman National Medical Library, Jerusalem, between 1978 and 1982 developed an integrated library system (MAIMON) on a minicomputer. Because humans are the most important element of the library system, MAIMON's performance was evaluated in terms of benefits provided to patrons, library management, and library staff. After successfully adopting the system, users' needs and expectations have grown. How the existing system will be used and expanded to meet the new information demands at the library is discussed.

Computers↗

PubMed1995

Developing a health sciences library Gopher: more involved than meets the eye.

The purpose of this paper is to discuss the development of the Health Sciences Library Gopher at St. Louis University (SLU) Health Sciences Center. The authors will outline university criteria from the Office of Computing and Information Services, library selection criteria, bibliographical access, reference and instructional implications, data entry and maintenance, and the role of the library's Internet Committee. All libraries have been awed by the tremendous amount of resources available through the Internet; the challenge is to not be intimidated but to be innovative in designing systems that can present these resources to the library client in the most easily understandable approaches possible. The Internet Committee in the SLU Health Sciences Center Library has designed a framework for the construction of "menus" to lead the client to selected Internet resources. Some of the issues confronted include relating the Internet resources to the library's Collection Development Policy, coordination of Internet resources to other bibliographic sources (Journal Holdings List, Intelligent Catalog), and educating staff and clients to manage a wide range of new resources as well as traditional ones in electronic formats.

Computer Communication Networks↗