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At least 649 records · Page 36Linked to original sources
PubMed1979

Glutathione reductase from human erythrocytes. Amino-acid sequence of a major fragment that links the FAD, NADP and interface domains.

A major CNBr fragment of glutathione reductase, peptide Q [Krohne-Ehrich, G., Schirmer, R.H. & Untucht-Grau, R. (1977) Eur. J. Biochem. 80, 65-71], was further fractionated by trypsin, chymotrypsin, thermolysin and clostripain digestion. The peptides were isolated and most of them were sequenced by solid-phase Edman degradation. The whole peptide Q was sequenced N-terminally up to position 51 by the same technique. A total sequence of 128 amino acids (28% of the whole protein) was obtained and could be localized in the electron density map [Schulz, G.E., Schirmer, R.H., Sachsenheimer, W. & Pai, E.F. (1978) Nature (Lond.) 273, 120-124] from position 259-387. This part of the polypeptide links and participates in all three domains of the flavoenzyme.

Amino Acid Sequence↗
PubMed1994

Two divergent MET10 genes, one from Saccharomyces cerevisiae and one from Saccharomyces carlsbergensis, encode the alpha subunit of sulfite reductase and specify potential binding sites for FAD and NADPH.

The yeast assimilatory sulfate reductase is a complex enzyme that is responsible for conversion of sulfite into sulfide. To obtain information on the nature of this enzyme, we isolated and sequenced the MET10 gene of Saccharomyces cerevisiae and a divergent MET10 allele from Saccharomyces carlsbergensis. The polypeptides deduced from the identically sized open reading frames (1,035 amino acids) of both MET10 genes have molecular masses of around 115 kDa and are 88% identical to each other. The transcript of S. cerevisiae MET10 has a size comparable to that of the open reading frame and is transcriptionally repressed by methionine in a way similar to that seen for other MET genes of S. cerevisiae. Distinct homology was found between the putative MET10-encoded polypeptide and flavin-interacting parts of the sulfite reductase flavoprotein subunit (encoded by cysJ) from Escherichia coli and several other flavoproteins. A significant N-terminal homology to pyruvate flavodoxin oxidoreductase (encoded by nifJ) from Klebsiella pneumoniae, together with a lack of obvious flavin mononucleotide-binding motifs in the MET10 deduced amino acid sequence, suggests that the yeast assimilatory sulfite reductase is a distinct type of sulfite reductase.

Amino Acid Sequence↗
PubMed2004

Participation of fad and mbt genes in synthesis of mycobactin in Mycobacterium smegmatis.

Colonies of Mycobacterium smegmatis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the accumulation in the cells of the deferri form of the siderophore mycobactin. Two mutants with little or no fluorescence, designated LUN8 and LUN9, were isolated by screening colonies of transposon (Tn611)-mutagenized M. smegmatis. Ferrimycobactin prepared from iron-restricted cells of the wild type had an R(f) of 0.62 on high-performance thin-layer chromatography (HPTLC) and a characteristic visible absorption spectrum with a peak near 450 nm. Similar extracts from LUN8 cells contained a small amount of ferrimycobactin with an R(f) of 0.58 on HPTLC and an absorption spectrum with the peak shifted to a wavelength lower than that of the wild-type ferrimycobactin. Nuclear magnetic resonance spectroscopy studies suggested that the LUN8 mycobactin may have an altered fatty acid side chain. Mutant strain LUN9 produced no detectable mycobactin. Neither mutant strain produced measurable amounts of excreted mycobactin, although both excreted exochelin (the mycobacterial peptido-hydroxamate siderophore), and both mutants were more sensitive than the wild-type strain to growth inhibition by the iron chelator ethylenediamine-di(o-hydroxyphenylacetic acid). The transposon insertion sites were identified, and sequence analyses of the cloned flanking chromosome regions showed that the mutated gene in LUN9 was an orthologue of the Mycobacterium tuberculosis mycobactin biosynthetic gene mbtE. The mutated gene in LUN8 had homology with M. tuberculosis fadD33 (Rv1345), a gene that may encode an acyl-coenzyme A synthase and which previously was not known to participate in synthesis of mycobactin.

Cloning, Molecular↗
PubMed1998

Fine mapping of 12 previously unassigned EST clones to individual YACs in the familial Alzheimer's disease (FAD) region of chromosome 14q24.3.

We have constructed an approximately 4-Mb YAC contig spanning the microsatellite markers D14S1028-D14S61 on chromosome 14q24.3 using oligonucleotide primers to microsatellite markers, ESTs and the genes PSEN1, FOS, TGFB3 and UBE2L1. This contig consists of 44 ICI YACs and 5 megaCEPH YACs. The data reported here should aid the final construction of an integrated map of chromosome 14 and allow the identification and accurate mapping of additional novel ESTs within this region.

Alzheimer Disease↗
PubMed1976

The behavior therapies: therapeutic breakthrough or latest fad?

The author critically examines the evidence for the effectiveness of the behavior therapies as described in the American Psychaitric Association's task force report on behavior therapy. He concludes that current attempts to evaluate behavior therapy suffer from inadequate methodology and that the claims for its therapeutic efficacy are excessive and unsupported by the controlled clinical evidence.

Behavior Therapy↗
PubMed1978

Popping and snorting volatile nitrites: a current fad for getting high.

In certain social circles a variety of volatile nitrites are being used extensively to expand creativity, stimulate music appreciation, promote a sense of abandon in dancing, and intensify sexual experience. Soon after amyl nitrite (the prototype of this group) became a prescription drug, legally produced substitutes appeared and were sold as room odorizers. Analyses of eight of these substitutes showed them to contain isomers of butyl or amyl alcohol and a corresponding nitrite. The authors describe the context of use of these products and their desired and adverse effects.

Amyl Nitrite↗