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PubMed1992

Regulation of estrus and ovulation in mares with progesterone or progesterone and estradiol biodegradable microspheres with or without PGF2alpha.

A single injection of a microsphere preparation, designed to deliver 1.25 gm progesterone and 100 mg estradiol-17beta at a controlled rate, for a duration of 12 to 14 days, produces accurate control of estrus and fertile ovulations in mares. Treatment is followed by PGF2alpha injection 14 days after steroid injection. The objectives of the present study were to determine whether estradiol added to the progesterone treatment or PGF2alpha administered at the end of the steroid treatment regimen, would improve synchronization of estrus and ovulation. A total of 45 cyclic horse mares was randomly assigned to 1 of 5 treatment groups as follows: Group 1 (control, n=9) sterile microsphere vehicle+sterile PGF2alpha vehicle 14 days after treatment with microsphere vehicle; Group 2 (n=9) progesterone and estradiol microspheres+PGF2alpha 14 days after treatment with microspheres; Group 3 (n=9) progesterone and estradiol microspheres+PGF2alpha vehicle 14 days after treatment with microspheres; Group 4 (n=9) progesterone + PGF2alpha 14 days after treatment with microspheres; and Group 5 (n=9) progesterone+PGF2alpha vehicle 14 days after treatment with microspheres. Addition of estradiol (P<0.05) or PGF2alpha (P<0.05) to the treatment regimen increased synchronization efficary by reducing variation in days to ovulation. All treatments significantly reduced variation in days to estrus compared with that of the controls; however, mares in the progesterone groups had an increased incidence of silent or shortened estrous behavior ( 2 days in duration compared with mares treated with progesterone (78 vs 33%, respectively). Therefore, estradiol and PGF2alpha each appear to reduce variation in days to ovulation while estradiol seems to promote better expression of posttreatment estrous behavior.

Journal Article↗
PubMed1994

Effect of service number on estrus duration in dairy goats.

The object of this research was to study the effect of sterile service number on estrus duration in dairy goats. Twenty-four Nubian goats (20 nulliparous and 4 multiparous) were randomly assigned to 1 of 4 treatment groups (n = 6 animals per group). The following Groups were formed: no service (GS-0); 1 service (GS-1); 2 services (GS-2); 3 services (GS-3). Estrus was synchronized by using fluorogestone acetate intravaginal pessaries (40 mg) over a 12-d period plus 400 IU im pregnant mare serum gonadotropin (PMSG) at pessary removal. Estrus was detected by using a vasectomized buck at 6-h intervals over 5 d after pessary removal (at 0600, 1200, 1800 and 2400 h). In the GS-0 group the teaser was outfitted with an apron and was permitted to mount. In the GS-1, GS-2 and GS-3 groups, the teaser was permitted to mount and service 1, 2 and 3 times, respectively, within the first 12 h after initiation of estrus. The duration of estrus for the 4 groups (GS-0, GS-1, GS-2 and GS-3) was (mean +/- SD) 41.0 +/- 5.9, 24.0 +/- 5.4, 22.0 +/- 4.9 and 22.0 +/- 7.2 h, respectively. These results show differences between the serviced groups and the nonserviced group (P<0.01), but they fail to show differences among the serviced groups (P>0.05). It is concluded that sterile service shortens estrus duration and that service number (1, 2 or 3) does not affect estrus duration.

Journal Article↗
PubMed2002

Influence of GnRH analogue (fertirelin acetate) doses on synchronization of ovulation and fixed-time artificial insemination in lactating dairy cows.

The effect of using a dose of 50 micro g rather than 100 micro g fertirelin in an ovulation/fixed-time insemination protocol for Holstein-Friesian dairy cows was investigated in three experiments. In each experiment, fertirelin was administered at the beginning of the protocol followed 7 days later by 500 micro g cloprosterol. Two days later, a second dose of fertirelin was given and AI performed 16-19 h later regardless of the incidence of behavioral oestrus. The effect on conception rate was studied in experiment 1 using 114 postpartum anoestrus cows. There was no significant difference in the age, parity or number of days after parturition in each treatment groups. The conception rate did not differ between the 50 micro g fertirelin group (61.1%; n=72) and the 100 micro g group (59.5%; n=42; NS). In experiment 2, a further 12 cows at 40-60 days postpartum were treated with 100 or 50 micro g fertirelin (n=6 per dose) with treatment commencing in the follicular or luteal phase of the oestrous cycle. The plasma concentration of luteinizing hormone (LH) reached similar peaks of over 5 ng/ml 120 min after the intramuscular administration of fertirelin in both groups. There were no significant differences in LH levels between treatments or phase of the oestrous cycle when treatment commenced. Doses of 50 and 100 micro g fertirelin were compared in experiment 3 using 17 cows to study follicular wave development and synchronization by transrectal ultrasonography, conception rate and corpus luteum function. There were no significant differences between treatments for these factors. It was concluded that using a dose of 50 micro g fertirelin enabled the drug costs to be reduced without affecting the efficiency of a synchronization of ovulation/fixed-time AI protocol for dairy cows.

Animals↗
PubMed1993

Effect of service on estrus duration in dairy goats.

The objective of this research was to determine the effect of sterile service on estrus duration in multiparous and nulliparous dairy goats. Twenty Nubian goats (10 multiparous and 10 nulliparous) were randomly assigned to of 4 treatment groups (n = 5 animals per group). Group MNS, multiparous without service; Group MS, multiparous with service; Group NNS, nulliparous without service and Group NS, nulliparous with service. Estrus was synchronized by utilization of fluorogestone acetate intravaginal pessaries (40 mg.) over a 12-day period plus 250 IU, i.m. of pregnant mare serum gonadotropin (PMSG) at pessary removal. Estrus was detected with the aid of a vasectomized buck for 5 days after pessary removal for 6-hour intervals (0600, 1200, 1800 and 2400 hours). In the groups that were not serviced the teaser was equipped with an apron and was only allowed to mount. In the serviced groups, the teaser was permitted to mount and service each female on 2 occasions during the first 12 hours of estrus. Estrus initiation for Groups NNS, NS, MNS and MS were (mean +/- SD) 61.5 +/- 29.5, 61.2 +/- 35.4, 63.0 +/- 22.2 and 69.6 +/- 32.5 hours, respectively (P>0.05). Estrus duration for the same groups were (mean +/- SD) 42.0 +/- 12.0, 30.0 +/- 6.0, 42.0 +/- 7.3 and 28.8 +/- 10.7 hours, respectively. These results show that estrus duration was shortened by serving (P<0.01), and that there were no differences between multiparous and nulliparous goats with or without serving (P>0.05). It is concluded that estrus duration in goats is shortened by serving and that no differences in duration exist between multiparous and nulliparous.

Journal Article↗
PubMed1996

Repeated matings with individual ewes by rams differing in sexual performance.

Twenty-four sexually mature rams were individually exposed to 10 estrous ewes on three occasions to determine the relationship between levels of sexual performance (ejaculation rate or serving capacity) and the incidence of repeated matings (ejaculations) with individual ewes. Rams that attained six ejaculations in a relatively short period of time (HP rams) mated fewer times with individual ewes than low-performing (LP) rams (P < .01). As a consequence, HP rams tended to mate with a greater number of different ewes than did LP rams (P < .06). In addition, time required to attain six ejaculations was inversely correlated with the number of different ewes mated (P < .03). The HP rams did not differ from LP rams in the number of different ewes investigated and courted. These findings highlight the value of using rams with relatively rapid ejaculation rates when breeding large groups of naturally cycling or synchronized ewes.

Animal Husbandry↗
PubMed2002

Effect of ram introduction on the oestrous cycle of springbok ewes (Antidorcas marsupialis).

Springbok are aseasonally breeding wild ungulates that inhabit arid environments, and interest has been shown in domesticating them for agricultural purposes. The present study was conducted for husbandry purposes to determine the effect of introducing a vasectomized ram to an isolated herd of springbok ewes (n = 9). Blood was collected from ewes every third day, before and after introduction of a vasectomized ram. Ewes were subjected to the ram for 42 days. Plasma progesterone was measured by radioimmunoassay and was used to establish the stage of the oestrous cycle. After introduction of the ram, the variation in the timing of the follicular phase between ewes was clearly reduced, compressing the spread of oestrus in the springbok ewes from 11 to 3 days. In seven of the nine ewes, the ram was introduced during the luteal phase of the oestrous cycle, causing this cycle to be significantly longer in duration (P < 0.05) and to have a higher maximum concentration of progesterone (P < 0.001) than cycles before and after introduction of the ram. This finding implies that the mechanism of synchronization operates through a luteotrophic effect. These results indicate that rams may be used successfully to synchronize breeding in springbok.

Adaptation, Physiological↗
PubMed1986

Progestin and prostaglandin for estrous synchronization in beef heifers.

Seventy-eight Simmental-Angus-Hereford crossbred yearling heifers, in 1983, and 99 similar heifers, in 1984, were used to compare two estrous synchrony regimens. One treatment group (SMB) was synchronized using the commercially available Syncro-Mate-B procedure, which involved placing a norgestomet implant in the ear for 9 d and giving an injection of norgestomet and estradiol valerate at the time of implantation. A second group (PR + PG) was given a norgestomet implant (PR) for 7 d and a 5-mg injection of alfaprostol (PG) at implant removal. Percentage of heifers cycling during the synchronization period and percent conceiving in 5 d or 30 d were not different (P greater than .10) due to treatment. The interval from implant removal to onset of behavioral estrus was shorter (P less than .01) for the heifers treated with SMB than for the heifers treated with PR + PG (42.8 vs 58.0 h). The group treated with SMB had a more uniform synchrony of estrus than the group treated with PR + PG. The effect of day of the estrous cycle at implantation on hours to estrus after implant removal was determined by a regression analysis, which showed a linear response for the SMB group with a slope of .78 (P = .09); the PR + PG group regression was cubic (P less than .01); this also indicated a more uniform response by the SMB group. These results indicate that the combination of norgestomet and alfaprostol produced more variation in interval from treatment to estrus than the Syncro-Mate-B procedure.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed2005

Pregnancy rates in lactating dairy cows after presynchronization of estrous cycles and variations of the Ovsynch protocol.

Our objectives were to determine pregnancy rates after altering times of the second GnRH injection, insemination, or both in a combined Presynch + Ovsynch protocol, to accommodate once-daily lockup of dairy cows. Lactating dairy cows (n = 665) from 2 dairy herds in northeastern Kansas were studied. Cows ranged from 24 to 44 d in milk (DIM) at the start of the Pre-synch protocol, which consisted of 2 injections of PGF(2alpha) 14 d apart, with the second injection given 12 d before initiating the Ovsynch protocol. Cows were blocked by lactation number and assigned randomly to 3 treatments consisting of variations of the Ovsynch protocol. Cows in 2 treatments received injections of GnRH 7 d before and 48 h (G48) after the PGF(2alpha) injection. Timed AI (TAI) was conducted at the time of the second GnRH injection (G48 + TAI48) or 24 h later (G48 + TAI72). Cows in the third treatment received the injections of GnRH 7 d before and at 72 h after PGF(2alpha) and were inseminated at the time of the second GnRH injection (G72 + TAI72). Pregnancy was diagnosed weekly by palpation per rectum of uterine contents on d 40 or 41 after TAI. Pregnancy rates differed between herds, but they were consistently greater for G72 + TAI72 than for G48 + TAI48 and G72 + TAI72. Subsequent calving rates were consistent with differences in initial TAI pregnancy rates. Pregnancy loss was least for cows on the G72 + TAI72 treatment. Body condition scores (BCS) ranged from 1.0 to 4.0 when assessed on Monday of the breeding week. An interaction of BCS and herd was detected in which cows in herd 1 having poorer BCS (<2.25) had greater pregnancy rates than cows of greater BCS (>/=2.25), whereas the reverse was true in herd 2 in which overall pregnancy rates were greater. We concluded that inseminating at 48 or 72 h after PGF(2alpha), when GnRH was administered at 48 h after PGF(2alpha), produced fewer pregnancies than inseminating and injecting GnRH at 72 h after PGF(2alpha) for cows whose estrous cycles were synchronized before initiating this variant of the Ovsynch protocol.

Animals↗
PubMed2006

Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of ovsynch in lactating dairy cows.

Ovulatory response to the first GnRH of Ovsynch is the critical determinant for successful synchronization of ovulation in dairy cows. Our objective in this study was to develop a pre-Ovsynch treatment that increased the percentage of cows that ovulated in response to the first GnRH injection of Ovsynch. To accomplish our goal, we evaluated a hormonal strategy that consisted of PGF2alpha and GnRH before the first GnRH of Ovsynch. Lactating dairy cows (n = 137) were assigned to receive either no treatment before Ovsynch (control) or 25 mg of PGF2alpha (PreP) followed 2 d later by 100 microg of GnRH (PreG), administered 4 (G4G), 5 (G5G), or 6 (G6G) d before initiating the Ovsynch protocol. Transrectal ultrasonography was performed to assess follicular size and resulting ovulation, and blood samples were collected to measure circulating concentrations of progesterone and estradiol immediately before each hormonal injection. Cows were inseminated at a fixed time 16 h after final GnRH of Ovsynch. Pregnancy diagnosis was performed 35 d later by palpation per rectum of uterine contents. Proportion of cows that ovulated to first GnRH of Ovsynch was 56.0, 66.7, 84.6, and 53.8% for G4G, G5G, G6G, and controls, respectively, and was greater for G6G than for control cows. Luteolytic response to PGF2alpha of Ovsynch was greater in all treated than control cows (92.0, 91.7, 96.2, and 69.2% for G4G, G5G, G6G, and control, respectively). Synchronization rate to Ovsynch was greater (92 vs. 69%, respectively) in G6G than in control cows. In addition, cows that ovulated in response to first GnRH of Ovsynch had greater response to PGF2alpha of Ovsynch (92.7 vs. 77.1%, respectively) and greater synchronization rate to the overall protocol (87.9 vs. 62.9%, respectively) than those that did not ovulate. Concentrations of progesterone at PGF2alpha of Ovsynch, and estradiol and follicle size at final GnRH of Ovsynch, were identified as significant predictors of probability of pregnancy 35 d after artificial insemination. In summary, a PGF2alpha-and-GnRH based pre-Ovsynch strategy consisting of a 6-d interval between PreG and first GnRH of Ovsynch resulted in a greater ovulatory and luteolytic response to first GnRH and PGF2alpha of Ovsynch, respectively, compared with control cows. This, in turn, optimized synchronization rate to Ovsynch.

Animals↗
PubMed1996

Effect of pronuclear DNA microinjection on the development of porcine ova in utero.

The objective of this study was to assess the effect of various aspects of pronuclear DNA microinjection on the early development of porcine ova in utero. Estrus was synchronized and superovulation was achieved in sexually mature gilts by the administration of allyl trenbolone, PMSG and hCG. Donor gilts were bred at 12 and 24 h after the onset of estrus. Ova were recovered between 60 and 62 h after the administration of hCG. One-cell ova that exhibited pronuclei after centrifugation were randomly allocated in equal numbers from each donor across one of two pairs of treatments: micro-DNA (ova were injected with two gene constructs that code for the human complement regulatory proteins decay accelerating factor and membrane cofactor protein) and control (ova were centrifuged only) or micro-buffer (ova were injected with buffer only) and pierced (a pipette was inserted into one pronucleus). Ova were transferred by treatment pairs to recipients. Treatments were segregated by oviduct. Ova were recovered after 120 h in utero, fixed and stained with 1% orcein. The proportion of ova that possessed > or = 80 nuclei, the mean number of nuclei present and proportion of ova that formed blastocysts were all significantly (P<0.05) greater for control and pierced ova than for micro-DNA and micro-buffer ova. No difference in these parameters was observed between micro-DNA and micro-buffer ova. These results demonstrate that pronuclear microinjection of a buffer alone can adversely affect the early development of porcine ova in utero.

Journal Article↗
PubMed1997

Ovulatory responses and plasma luteinizing hormone concentrations in dairy heifers after treatment with exogenous progesterone and estradiol benzoate.

The objectives of this experiment were to determine the effects of 0.5 mg estradiol benzoate, administered intramuscularly 24 h after removal of CIDR-B progesterone containing intravaginal devices, on the time to estrus, ovulation and peak LH concentration in dairy heifers. Ovulatory responses and plasma LH concentrations were examined using 14 Friesian dairy heifers in 2 separate treatment periods. All heifers received a CIDR-B progesterone-containing intravaginal device with an attached 10-mg estradiol benzoate capsule for 12 d. Within each period, 24 h after CIDR-B removal, 7 heifers received an intramuscular injection of 0.5 mg estradiol benzoate while the remaining 7 heifers received an intramuscular injection of a placebo. Blood samples for LH assay were collected at 0, 6 and 12 h, and then every 4 h for 60 h after estradiol injection. Detection of estrus was conducted at 4-h intervals, and ultrasonographical examination to detect ovulation was conducted every 8 h for 88 h after removal of the CIDR-B device. Treatment with estradiol benzoate tended to reduce the time from device removal to the LH peak in Period 1 (median time to LH peak 40.1 vs 63.9 h; P = 6.07). In Period 2, treatment with estradiol had no significant effect on the time to the LH peak, standing estrus or ovulation. We hypothesize that the period effect was due to the stage of cycle at the time of treatment. For heifers treated in Period 1, the stage of cycle was random. However, because of the prior synchronization of estrus, which was implicit in the experimental design, heifers in Period 2 tended to be in late diestrus. The administration of estradiol benzoate after treatment with exogenous progesterone appears to overcome the variability in timing of LH peaks typically occurring in a herd of synchronized heifers due to different stages of follicular development.

Journal Article↗