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PubMed1996

Lectin-like receptor for alpha 1-acid glycoprotein in the epithelium of the rat prostate gland and seminal vesicles.

BACKGROUND: A receptor for alpha 1-acid glycoprotein glycoforms AGP-B and AGP-C in the epithelium of the rat prostate gland and seminal vesicles is described. METHODS: The interaction between AGP-glycoforms and their receptor is a lectin-like interaction confirmed by inhibition of the binding by mannose and N-Acetyl-D-glucosamine. RESULTS: In vitro the receptor was also inhibited by the steroid hormones cortisone, aldosterone, progesterone, and estradiol, but not by testosterone. A significant regional variation in the expression of AGP-lectin receptor and in the localization of AGP was seen in rat prostate and seminal vesicles. The localization of the AGP lectin receptor is compared to the localization of glycoprotein AGP, and small differences are found. CONCLUSIONS: It is proposed the AGP receptors in the prostate and seminal vesicles belong to a group of lectins in the control of differentiation and organ formation.

Aldosterone↗
PubMed1983

Effect of selenium on the subcellular distribution of glutathione peroxidase in rat liver, epididymal fat pad and seminal vesicle.

The effect of dietary selenium on the subcellular distribution of selenium-dependent glutathione peroxidase (SeGSH-Px) activity in rat liver, epididymal fat pad, and seminal vesicle was determined. Tissues were fractionated by differential centrifugation, and the subcellular distribution of SeGSH-Px activity was determined by comparison with the distribution of biochemical marker enzymes. Liver SeGSH-Px activity was located in both the cytosol and mitochondria. In epididymal fat pad and seminal vesicle, SeGSH-Px activity was located primarily in the cytosol; association with another subcellular organelle, however, was indicated. In liver and epididymal fat pad, SeGSH-Px activity increased linearly, and, in the seminal vesicle, increased linearly and quadratically, with increasing dietary selenium concentration. Distribution of SeGSH-Px activity among the cellular fractions from the tissues, however, was not affected by dietary selenium supplementation.

Adipose Tissue↗
PubMed1990

Biological functions of mouse seminal vesicle fluid. I. Suppression of blastogenic responses of lymphocytes.

The effect of mouse seminal vesicle fluid (SVF) on blastogenic response of splenocytes to mitogens was investigated. SVF significantly suppressed blastogenic response of splenocytes to concanavalin A and phytohemagglutinin in a dose-dependent manner, but blastogenic response to lipopolysaccharide was suppressed only at low, although significant, levels, even at high concentrations of SVF. Extensive dialysis did not reduce the capacity of SVF to inhibit blastogenesis of splenocytes. For elucidation of the mechanisms of suppression of blastogenic response, interleukin-2 (IL-2)-dependent cells were cultured in the presence of IL-2 and various concentrations of SVF. The presence of SVF did not inhibit the proliferative response of IL-2-dependent cells to IL-2. These results suggest that the suppression of blastogenic response of T lymphocytes to mitogens in seminal plasma is caused by an undialyzable component (or components) derived from seminal vesicle and is attributable to the alteration of receptors for mitogens or of IL-2 receptors that are expressed on stimulation by mitogens.

Animals↗
PubMed1996

Increased secondary sex ratios in golden hamster litters sired by males without coagulating glands and seminal vesicles.

In golden hamsters, although bilateral ablation of paternal coagulating glands (CGX) and seminal vesicles (SVX) did not affect fertility, a higher number of male pups were born. The present study aimed at determining whether this male-biased sex ratio was due to an imbalance of fertilization by X and Y chromosome-bearing sperms or whether it was the consequence of a sex-related differential survival of embryos. The sex of embryos sired by sham-operated (SH) controls or males subjected to bilateral ablation of ampullary glands (AGX), CGX and SVX was determined from chromosomal spreads at 10 h post coitum and 10 days post coitum. The primary sex ratio of of the SH group did not deviate from the hypothetical sex ratio of 1:1. The sex ratios of zygotes from the three experimental groups did not differ from that of the controls. However, by mid gestation, the sex ratio was significantly higher in the SVX group (P < 0.05) and the CGX group (P < 0.005). The absence of secretions from the ampullary gland, coagulating gland and seminal vesicle had no effect on the primary sex ratio, thus these glands did not appear to affect fertilization by the X and Y chromosome-bearing sperm. The increased secondary sex ratios observed in the SVX and CGX groups were due to the preferential survival of males.

Animals↗
PubMed1993

KCl contractions in the rat isolated seminal vesicle.

Potassium chloride (K+) produced dose-dependent contractions of the rat isolated seminal vesicle with no sign of tachyphylaxis. The contractile response was biphasic in nature and composed of an early rapid phasic contraction and a slowly developing, but more sustained, tonic response separated by a transient relaxation. Neither cocaine nor depletion of tissue catecholamines by reserpine influenced responses to K+. Moreover, guanethidine, phentolamine, phenoxybenzamine, atropine and physostigmine all failed to modify responses of the tissue to K+. Thus, the possibility of K+ acting via release of endogenous noradrenaline or acetylcholine is excluded. Calcium-free conditions with or without EGTA reduced both the components of K(+)-induced contraction. The rate of reduction of both responses was faster in the presence of EGTA with part of the tonic response being resistant even to calcium deprivation in the presence of EGTA. On the other hand, verapamil reduced both responses in a similar manner, whereas nifedipine produced dose-dependent rightward shifts of the concentration-response curves of both the phasic and tonic responses to K+. However, in the presence of nifedipine, the maximum response of only the phasic contraction was significantly lowered. It is concluded that both phases of KCl contraction in the rat seminal vesicle use extracellular Ca2+, of which some is tightly bound with high affinity, probably to plasma membranes. In addition, two subtypes of voltage-sensitive Ca2+ channels may exist, one of which is preferentially sensitive to nifedipine and both are sensitive to verapamil.

Acetylcholine↗
PubMedDate not supplied

Preliminary observations on steroid metabolism in isolated epithelum of guinea pig seminal vesicle.

The metabolism of nine radioactively labeled steroids in the epithelium of the seminal vesicle of the mature guinea pig has been studied. The rapid assimilation and metabolism of these steroids demostrate the very active biochemical nature of this tissue. Based on the use of several thin-layer chromatography systems and comparison with the locations of known standards, the following have been disclosed. Testosterone was rapidly converted to dihydrostestosterone and androstanediol. The latter was the major metablolite of dihydrotestosterone and of androsterone. Androstenedione was readily converted to androstanedione, testosterone, and dihydrostestosterone, although it formed little androstanediol. Dehydroepiandrosterone was converted to small amounts of androstenediol and androstanediol. Pregnenolone was rapidly converted to an unidentified highly polar compound only. Progesterone was converted to 5alpha-pregnane-3beta-ol-20-one and its 3alpha-isomer. Dehydroepiandrosterone and progesterone were also significantly converted to unidentified highly polar compounds. The major metabolites of 17-hydroxyprogesterone co-chromatographed with standard androstanediol, testosterone, and unidentified metabolite possessing intermediate chromatographic mobility. In addition, 17-hydroxyprogesterone was converted to small amounts of compounds possessing identical Rf values as standard androstenedione and dihydrostestosterone. The identification of the products of 17-hydroxyprogesterone metabolism and their physiologic significance must await critical evaluation. Because of its homogeneity, isolated epithelium of guinea pig seminal vesicle shows promise as a tissue preparation for use in future studies that might elucidate the role(s) of individual androgens in secretory tissues of the male accessory sex organs. Our demonstration of extensive steroid interconversions in this tissue is a logical prerequisite to such studies.

Androgens↗
PubMed1993

Protease-nexin I as an androgen-dependent secretory product of the murine seminal vesicle.

A search for inhibitors of urokinase-type plasminogen activator (uPA) in the male and female murine genital tracts revealed high levels of a uPA ligand in the seminal vesicle. This ligand is functionally, biochemically and immunologically indistinguishable from protease-nexin I (PN-I), a serpin ligand of thrombin and uPA previously detected only in mesenchymal cells and astrocytes. A survey of murine tissues indicates that PN-I mRNA is most abundant in seminal vesicles, where it represents 0.2-0.4% of the mRNAs. PN-I is synthesized in the epithelium of the seminal vesicle, as determined by in situ hybridization, and is secreted in the lumen of the gland. PN-I levels are much lower in immature animals, and strongly decreased upon castration. Testosterone treatment of castrated males rapidly restores PN-I mRNA levels, indicating that PN-I gene expression is under androgen control.

Amyloid beta-Protein Precursor↗
PubMed1997

[Seminal vesicle cyst associated with homolateral renal agenesis and megaureter. Apropos of a case].

We report a case of a 24-year-old male patient with cystic dilatation of the seminal vesicle associated with renal agenesis and megaureter. This patient complained of left renal colic with pollakiuria. We performed intravenous urography and ultrasound showed a cyst in the seminal vesicle associated with left megaureter and right renal agenesis. Treatment consisted of resection of the cyst and reimplantation of the left ureter into the bladder. No postoperative complications were observed. This association is rare and can be explained by the common embryological origin of the seminal vesicle and kidney and ureter. Its diagnosis is now facilitated by ultrasound and CT. The treatment of this tumor is surgical only when the lesions are responsible for clinical symptoms.

Adult↗
PubMed2003

Regulatory effect of castration on endothelins, their receptors and endothelin-converting enzyme in rat seminal vesicle.

OBJECTIVE: To investigate the effects of castration on the expression of endothelins (ETs), ET receptors and ET converting enzyme-1 (ECE-1) in the rat seminal vesicle (RSV). MATERIALS AND METHODS: Sprague-Dawley rats (3 months old) were surgically castrated or sham-operated, and then killed 7 days after surgery. Biochemical and pharmacological properties and the location of ET receptors in the RSV were determined by a series of binding experiments with [125I]ET-1, using membrane particulates and slide-mounted frozen sections of RSV. Expression levels of ETA and ETB receptor subtypes, ET-1, ET-3 and ECE-1 mRNAs were assessed by relative multiplex reverse-transcription polymerase chain reaction (RT-PCR). RESULTS: The density of total ET receptors increased significantly in the seminal vesicle of the castrated rat. The predominance of the ETA receptor subtype in the RSV did not change with castration. Autoradiographic studies showed the presence of ET receptors on the smooth muscle and epithelium of the RSV. In addition, RT-PCR showed an up-regulation in the expression of ETA and ETB receptor subtypes, ET-1 and ECE-1 mRNAs in the seminal vesicle of the castrated rat. However, castration caused no significant change in the expression levels of ET-3 mRNA. CONCLUSION: These findings suggest a regulatory role for testosterone in the expression of the ET receptor system in the RSV.

Animals↗
PubMed1992

Cystadenoma of the seminal vesicle: case report and literature review.

A benign fibroepithelial and cystic tumor of the seminal vesicle is described in a 63-year-old man. The symptoms, macroscopic appearance, microscopic characteristics, immunophenotype prognosis and complications of this rare benign tumor are discussed, and 9 previously reported cases are reviewed. Several terms have been used to describe benign fibroepithelial tumors of the seminal vesicle. We suggest that the term cystadenoma is the most appropriate.

Cystadenoma↗
PubMed1978

Effects of testosterone on messenger ribonucleic acid and protein synthesis in rat seminal vesicle.

In a previous report [Higgins et al. (1976) Biochem. J.158, 271-282] we described the effects of alterations in androgen status on the synthesis of two basic secretory proteins of the rat seminal vesicle. In the present paper we examine the effects of testosterone on the activity of mRNA in the seminal vesicle. Total cellular poly(A)-rich RNA was isolated and translated in a cell-free system prepared from wheat germ. Translation products were separated on denaturing polyacrylamide gels and the protein bands corresponding to the two basic secretory proteins were identified immunologically. Incorporation of radioactive methionine into these bands was taken as a measure of the individual mRNA activities. Total mRNA activity was estimated by radioactivity in total acid-precipitable material. The results show that 1 to 2 weeks after castration the activities of mRNA molecules for the basic secretory proteins were decreased 10-20-fold on a tissue basis. Testosterone given in vivo rapidly and substantially restores mRNA activity to normal. Since these changes correlate closely with variations in the rates of synthesis of the secretory proteins in whole cells it suggests that androgenic steroids control protein synthesis chiefly via mRNA availability. In this respect their action resembles those of other steroid hormones acting in other systems. However, these effects of testosterone on the mRNA molecules for the major secretory proteins could not be distinguished from those on total mRNA. Thus the proportion of the total mRNA population accounted for by the two specific mRNA molecules showed less than a 2-fold variation with androgen status. Similarly the two secretory proteins always accounted for 25-33% of general protein synthesis. This is in sharp contrast with the markedly differential effects of other steroid hormones controlling synthesis of major proteins in other well-studied systems. We interpret our results as indicating that testosterone regulates the mRNA population of the seminal vesicle as a whole.

Animals↗
PubMed1992

Papillary adenocarcinoma in a seminal vesicle cyst associated with ipsilateral renal agenesis: a case report.

Cysts and tumors of the seminal vesicle are uncommon, and their coexistence is extremely rare. We report a case of multiple papillary tumors inside a seminal vesicle cyst associated with ipsilateral renal agenesis in a 17-year-old man. Surgical excision of the cyst and tumors was performed without any morbidity and histology revealed well differentiated papillary adenocarcinoma.

Adenocarcinoma, Papillary↗
PubMed1993

Expression of sulfated glycoprotein 2 is associated with carcinogenesis induced by N-nitroso-N-methylurea in rat prostate and seminal vesicle.

To understand the molecular mechanism of carcinogenesis in androgen-dependent tumors, we have searched for new markers which are associated with this process. In normal rat prostate and seminal vesicle, sulfated glycoprotein 2 (SGP-2) messenger RNA is barely detectable. However, we have found high levels of SGP-2 expression in the epithelial component of carcinomas of the prostate and seminal vesicle after initiation with N-nitroso-N-methylurea and promotion with testosterone propionate. We have also observed induction of SGP-2 expression in epithelial cells at early stages in carcinogenesis when cytologically malignant cells first begin to appear. SGP-2 has been reported previously to be associated with a variety of models of programmed cell death (apoptosis), including the prostate following castration. Our present findings provide a novel marker for carcinogenesis in the rat prostate and seminal vesicle.

Animals↗
PubMed1978

Seminal vesicles as organs of sperm storage.

This study was done to determine whether or not the seminal vesicles store spermatozoa. Half of the nonhuman primates' vesicles had sperm at autopsy as did one of the men dying a traumatic death. Seminal vesicle sperm storage may be the reason for continued spermatozoa in the ejaculates of men after vasectomy.

Adolescent↗
PubMedDate not supplied

[Retroiliac ectopic ureter opening to the seminal vesicle].

OBJECTIVE: To describe a case of right retroiliac ectopic ureter opening into the seminal vesicle, an uncommon congenital malformation. METHODS/RESULTS: This anomaly was diagnosed in a 24-year-old male with recurrent acute right epididymitis based on the clinical, ultrasound and CT findings. Cystoscopy and IVP provided no additional information. Surgical exploration showed right renal agenesis and a retroiliac ectopic ureter opening into a dilated right seminal vesicle. These structures were resected. Patient evolution was satisfactory with no recurrence of the infective episodes. CONCLUSIONS: Diagnosis of this anomaly is based on clinical suspicion and the findings of the diagnostic imaging techniques. Surgical exploration, which is only required in symptomatic patients, will establish the correct diagnosis and treatment.

Abnormalities, Multiple↗
PubMed1993

Thiamine pyrophosphatase activity in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with oestradiol.

Ultrastructural localization of thiamine pyrophosphatase (TPPase) activity was studied in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with 17 beta-oestradiol benzoate. The present study has demonstrated that TPPase reaction product is consistently localized in the three to four trans cisternae of Golgi complexes in both the lateral prostate and the seminal vesicle. The reaction was intense and the reaction product often filled the cisternae completely. After castration there was a decrease in TPPase activity in both glands as revealed by the reduction in the amount of the reaction product which was found mainly in one to two trans cisternae of the regressed Golgi complex. The reaction product changed from a dense to a more particulate or granular pattern or to discrete deposits of high electron-density. Administration of 17 beta-oestradiol benzoate to the castrates caused changes in the localization and patterns of distribution of TPPase. In the lateral prostate there was an apparent increase in TPPase activity. The reaction product was found in two to four trans cisternae and occasionally in the trans-most cisternae of the dilated Golgi complex. The reaction product appeared as discrete, dense coarse precipitates. In the seminal vesicle TPPase reaction product was consistently found in one to two trans cisternae in cells with larger Golgi complexes. However, almost all cisternae of the smaller Golgi complexes were TPPase-positive.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1984

Heterogeneity and distribution of estrogen binding sites in guinea pig seminal vesicle.

Cytosol fractions from the separated epithelium and fibromuscular stroma of the guinea pig seminal vesicle exhibit two types of [3H] 17 beta-estradiol binding. High affinity, low capacity, estrogen-specific sites are observed in each component. In addition, secondary binding sites with moderate affinity (Kd = 35 nM) and high capacity (500 fmol/mg cytosolic protein) are also observed. In epithelial cytosol, 10 mM sodium molybdate or 1 mM dithiothreitol enhance [3H] 17 beta-estradiol binding to the secondary sites. In stromal cytosol, sodium molybdate is without effect while dithiothreitol completely abolishes binding of [3H] 17 beta-estradiol to the secondary sites. Specificity studies indicate that [3H] 17 beta-estradiol binding to the secondary sites in stromal cytosol is inhibited by estrogens, but not by androgens. In epithelial cytosol estrogenic specificity is not evident. These results demonstrate the existence of [3H] 17 beta-estradiol binding sites in the fibromuscular stroma which are similar to the Type II estrogen binding components present in rat uterus and prostate. The secondary sites in epithelium may in part, represent binding of [3H] 17 beta-estradiol to another steroid hormone receptor present in this tissue component. These results also suggest a possible mechanism which may account for the known sensitivity of the seminal vesicle fibromuscular stroma to estrogen stimulation.

Animals↗