Changes in hepatic enzyme levels after adrenalectomy. I. Phosphorylase, phosphoglucomutase, and phosphoglucoseisomerase.
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For the routine subtyping of PGM1 isozymes by means of agarose gel thin-layer electrophoresis, a tris-maleic acid-buffer and an acetic acid-buffer, several agaroses with electroendosmosis up to -0, 16, and several papers are tested. With all these materials good results can be obtained, so that agarose without electroendosmosis is not necessary and FN 1 papers can be used.
1315 school-boys of the Cosenza province (Calabria - southern Italy) were subtyped for the PGM1 locus. Three subsamples were examined, two from the Tyrrhenian and the Ionian coasts, respectively, and one from the internal part of this province. The PGM1*1S, PGM1*2S and PGM1*2F gene frequency estimates obtained for the population of the Ionian coast were significantly different from those of the tyrrhenian coast. Since the three subsamples were of similar size, estimates weighted also with the respective population sizes were calculated.
Two small populations of inner French Guiana were investigated for red cell enzymes and serum proteins. Rare variants were found in four systems (AK1, PGM1, PGM2, Tf). Similarities between some of these variants and those found in other Amerindian populations indicate that these genetic markers may be valuable in studies of Amerindians. A mutation rate has been estimated from the results.
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