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[Pharmacognostic studies on mangrove Acanthus ilicifolius].

OBJECTIVE: In order to prove a basis for rational exploitation and utilization of the herb, the charaeteristic feature for Acanthus iliciflius was studied. METHOD: The botanical and pharmacognostic features of the collected original plant were stadied. RESULT: A. ilicifolius showed obvious pharmacognostic characters. The aerate cells of the root were upgrowth. and four vascular bundles were found in leaf venation. CONCLUSION: The distinct characteristic features revealed in this studies can serve as evidence for the identification of A. ilicifolius.

Acanthaceae↗

[Discussion on criterions of endanger and protection levels of traditional Chinese medicine herbs].

To efficiently protect the endangered traditional Chinese medicine herbs is essential for the sustainable development of traditional Chinese medicine. On the bases of present species endanger and protection levels, problems in the traditional Chinese medicine herbs are analyzed. The endangered levels of traditional Chinese medicine herbs should refer to the standard of IUCN, and the protection levels should adopt qualitative and quantitative analysis based on the characteristic of traditional Chinese medicine herbs. Some qualitative and quantitative factors are discussed, some useful information was also provided for the establishment of protection levels of traditional Chinese medicine herbs.

Conservation of Natural Resources↗

[Study on identification of wild and cultirated Radix Scutellariae in different growing years].

OBJECTIVE: To identify Radix Scutellariae (Huangqin) of different growth years, to distinguish whether it's wild or cultivated and to provide useful information for the quality control of Huangqin crude drug. METHOD: By using morphological and histological methods, we studied 87 individuals of 45 specimens from 12 habitats of 5 provinces of China, which grew wild or were cultivated in different growing years. Moreover, 22 commercial samples of Huangqin from 7 provinces were also investigated. RESULT: The identification was performed base on morphological and histological characteristics, such as, the shape, color, cork, remaining stems, decayed central xylem, and vessels arrangement, xylem cork ring, growth rings, etc. CONCLUSION: We established an identification method for distinguishing Huangqin wild or cultivated in different growing years. Furthermore, the structure of annual rings in the transection of Radix Scutellariae has been discovered for the first time.

China↗

[Study on circumscription of medicinal licorice species based on molecular biology].

OBJECTIVE: Elucidate the medicinal licorice species circumscription in order to develop licorice resources in China and select new cultivars. METHOD: PCR amplification, DNA sequencing and cladistic analysis. RESULT: Acquire the ITS sequences and phylogenetic tree of 8 licorice species. CONCLUSION: According to results Glycyrrhiza eurycarpa and G. glabra var. glandulosa should be combined into G. and G. glabra respectively and G. egladulosa is an independent species.

Base Sequence↗

[The application of DNA molecular markers in conservation of the rare and endangered medicinal plants].

In this paper, the advance in DNA molecular markers techniques in recent years was reviewed. The application of DNA markers in conservation of the rare and endangered medicinal plants was explicated, of which included identification of germ-plasm resource, determination of the habitats unite which should be protected in situ, sampling strategies of ex-situ conservation, evaluation of the conservation effects of the rare and endangered medicinal plants, as well as elucidation of their endangered mechanism etc. The information could help drawing up conservation strategies and conservation measures for references.

Conservation of Natural Resources↗

[Progress and prospect of botancial research in Dioscorea zingiberensis].

This paper is a summary of the progress of botanical research in Dioscorea zingiberensis. It contains biological characteristic, genetic diversity, growth circumstance, breeding, cultivating, tissue and cell culture of D. zingiberensis. According to the fact of market demanding and resources protection, the writers propose that some work, including establishing gene bank, resources appraise, employing advanced technology to utlize the resources and making the cultivated standard, should be taken into consideration.

Cell Culture Techniques↗

[Corrections on medicinal resource of Gramineae in Guizhou province and Guiyang city].

OBJECTIVE: To confirm the medicinal plants of Gramineae and their distribution in Guiyang city. METHOD: Through field investigations and comparing the collected speciments and literatures, the geographical distribution and functions of the medicinal species were investigated. RESULT: 24 new medicinal species of Gramineae were found in Guizhou, while 5 species were absent in Guizhou. And 1 was first recorded for medicinal uses, 3 species newly added, and the latin name of 1 species was corrected. CONCLUSION: There are 72 medicinal species and 4 varieties in Guiyang.

China↗

[Study on resource and varieties of Guoshangye].

OBJECTIVE: To investigate the varieties, resoures and identification authentication of Guoshangye used in Tuja and Miao's folk. METHOD: Through field investigations and comparing the collected specimens and literatures, the classification and identification of the species were studied. RESULT AND CONCLUSION: Origin of Guoshangye plant was from Pholidota yunnanensis, Bulbophyllum andersonii, B. odoratissimum, B. kamgtimgemse, ect. their morphological characters were identified. Due to limited resource of Guoshangye, the development and protection should be paid more attention.

Analgesics↗

[Pharmacognostical identification of Vernonia patula (Dry) Merr].

OBJECTIVE: To identify Vernonia patula (Dry) Merry. METHODS: Characteristic study, microscopic identification and UV spectrometry method were carried out. RESULTS: Obvious characteristics were found in the tissue structures of the roots, stems and leaves. CONCLUSION: The results can be taken as the reference for analyzing the quality of the crude drug.

Pharmacognosy↗

[Identification on Spora Lygodii by X-ray diffraction fingerprint spectra method].

OBJECTIVE: To set up a new identification and analysis method of Spora Lygodii. METHOD: To get the extracts of Spora Lygodii using methanol, chloroform and petroleum ether as solvent and the extracts were identified, analyzed by X-ray diffraction Fourier fingerprint spectra. RESULTS: Experiments and analysis were carried out on three samples. The standard X-ray diffraction Fourier fingerprint spectra and characteristic diffraction peaks were obtained. There were some differences among the spectra of the extracts, but the characteristic diffraction peaks were obvious. CONCLUSION: The experimental result indicated that X-ray diffraction Fourier fingerprint spectra can be used to identify and analyze the Chinese traditional herb Lygodium japonicum (Thunb.) Sw.

Ferns↗