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Modification and expansion of a pure oxygen WWTP for biological nutrient removal (BNR).

A pure oxygen activated sludge system was converted to a VIP configuration BNR (biological nutrient removal) system wherein three of the five pure oxygen sections were retained, and performance was compared to that of a side-by-side air aeration MUCT (modified UCT) system. Because the pure oxygen BNR system could not obtain good nitrification, its treatment capacity had to be downgraded from 113,550 m3/d to a flow of only 60,000 m3/d. At the lesser flow, it was determined that adequate nitrification and improved denitrification could be accomplished in the pure oxygen system by continuously seeding it with 100% of the WAS from the MUCT system. Fortunately, while the capacity of the pure oxygen system had to be downgraded, it was determined that the capacity of the MUCT system was substantially greater than its design flow, and the combined system is capable of treating the entire design flow. However, this requires increasing the operating sludge age of the MUCT system. The pure oxygen BNR system performed better phosphorus removal than the MUCT system, both before and after seeding with the MUCT WAS. Apparently this was because the MUCT system was operated at a substantially higher sludge age than the pure oxygen system. However, both systems have consistently discharged effluent phosphorus concentrations of less than 2.0 mg/L TP, which is the Chesapeake Bay standard. Even with improved nitrification and denitrification in the pure oxygen BNR system, neither it nor the MUCT system have proven to be capable of meeting the Virginia Chesapeake Bay goal of 10 mg/L total nitrogen in the effluent.

Bioreactors↗

The nature of nitrogen: an overview.

Most of the nitrogen available to the biosphere exists as N2 in the atmosphere, and is not useful to most organisms until it is "fixed" either biologically or abiotically (by lightning or aurorae, or industrially). Once it is fixed into NH3, usually it is either assimilated and transformed into organic N or nitrified into NO3-. Organic N can be transformed back into NH3 by ammonification. Nitrate can be converted into N2O by nitrification and denitrification, and to N2 by denitrification. Such N2O and N2 production results in nitrogen loss from ecosystems and a nitrogen gain to the atmospheric nitrogen reservoir. The different steps of the nitrogen cycle require different environmental conditions. These differences result in a spatial distribution pattern of the different nitrogen transformation reactions. Biological nitrogen fixation occurs universally in soils, sediments, fresh water, and marine systems that are both aerobic and anaerobic. Nitrification occurs primarily in aerobic habitats, whereas denitrification predominates in anaerobic habitats such as sediments and water-logged soils. In closed systems, such as plant growth chambers for CELSS, denitrification and nitrification result in a loss of fixed nitrogen available to plants.

Ammonia↗

In situ identification of azo dye inhibition effects on nitrifying biofilms using microelectrodes.

In this study, the inhibitory effects of acid orange 7 (AO7), a common azo dye, on nitrification in biofilms were investigated in situ using microelectrodes. Biofilms were obtained from laboratory rotating drum biofilm reactor after the nitrification process reached a pseudo-steady state. Dissolved oxygen, pH, NH4+, NO3-, and redox potential microelectrodes, with tip diameters ranging from 3-15 microm, were used to monitor the spatial distribution and change of microbial activities within nitrifying biofilms. It was found that at lower concentration (1 mg/L), AO7 had only a slight impact on the NH4+-N concentration profiles. The ammonium consumption rate decreased as higher AO7 concentrations (15 mg/L and 25 mg/L) were exposed to the biofilms. A similar trend was observed for the NO3(-)-N microprofiles. The nitrate production rate decreased as the AO7 concentration in the bulk solution increased. The dissolved oxygen and pH microprofiles also showed oxygen and alkalinity utilization, but at lower rates throughout the biofilms when the nitrification process was inhibited. No significant redox potential differences were observed in the biofilms after AO7 was applied.

Azo Compounds↗

Nitrifying microbial community analysis of nitrite accumulating biofilm reactor by fluorescence in situ hybridization.

Biological nitrogen removal via nitrite pathway in wastewater treatment is very important especially in the cost of aeration and as an electron donor for denitrification. Wastewater nitrification and nitrite accumulations were carried out in a biofllm reactor. The biofilm reactor showed almost complete nitrification and most of the oxidized ammonium was present as nitrite at the ammonium load of 1.2 kg N/m3/d. Nitrite accumulation was achieved by the selective inhibition of nitrite oxidizers by free ammonia and oxygen limitation. Nitrite oxidation activity was recovered as soon as the inhibition factor was removed. Fluorescence in situ hybridization studies of the nitrite accumulating biofilm system have shown that genus Nitrosomonas which is specifically hybridized with probe NSM156 was the dominant nitrifying bacteria while Nitrospira was less abundant than those of normal nitrification systems. Further FISH analysis showed that the combinations of Nitrosomonas and Nitrospira cells were identified as important populations of nitrifying bacteria in an autotrophic nitrifying biofilm system.

Biofilms↗

[Pilot study on subsurface wastewater infiltration system applied in rural sewage treatment].

A pilot plant of subsurface wastewater infiltration system (SWIS) filled with red clay was tested to treat rural sewage with hydraulic loading of 2 cm/d. The experiment results showed that average removal rates of COD, NH4(+)-N, total phosphorus, and total nitrogen were 84.7%, 70.0%, 98.0% and 77.7%, with average effluent concentrations of COD, NH4(+)-N, total phosphorus, and total nitrogen being 11.7 mg/L, 4.0 mg/L, 0.04 mg/L and 4.7 mg/L respectively, which met the standard for water reuse issued by the Ministry of Construction of China. Nitrogen removal mechanism analysis revealed that nitrogen in the influent was mainly eliminated through biological removal via nitrification/denitrification processes. In the pilot system, denitrification was well proceeded but nitrification was not satisfactory. How to improve nitrification performance through change of soil environment was the key to enhance nitrogen removal rate. The measurement results of redox potential in the filled soil showed that reductive property of soil was main hindrance to nitrification process.

Filtration↗

[Nitrogen removal from the sewage containing seawater via nitrite pathway].

With control of the concentration of free ammonia (FA), shortened nitrification-denitrification was accomplished in SBR to achieve enhanced nitrogen removal from the sewage containing seawater. Several parameters which included salinity, temperature, pH and NH4(+)-N load were studied to evaluate their effects. The results of experiences indicated that shortened nitrification-denitrification can be accomplished in sewage containing seawater with relatively high salinity. With various salinity, the nitrogen removal efficiency had relationship with the NH4(+)-N load, there should be a lower NH4(+)-N load when the salinity was high. The nitrogen removal efficiency reached above 90% when the NH4(+)-N load was not exceed 0.15 kg/(kg.d). Elevation of the temperature availed to higher nitrogen removal efficiency, this efficiency doubled when the reaction temperature was changed from 20 degrees C to 30 degrees C. Relatively high value of pH, in the range of 7.5 and 8.5 had advantage to achieve effective shortened nitrification-denitrification which caused by the selective inhibition of free ammonia(FA).

Nitrites↗

Controlling a combined lagoon/reed bed system using the oxidation-reduction potential (ORP).

Lagoon systems achieve good and stable effluent data in regard to organic pollutants, but they charge the receiving waters with relatively high ammonium loads. Therefore an existing lagoon-plant was extended by a vertical flow reed-bed for the special purpose of nitrification. This paper presents the efficiency of the combination plant as well as the possibility to monitor and control the reed-bed operation by the oxidation-reduction potential (ORP). The results show that the combination plant achieved excellent purification results, the average efficiency degrees were 97% for COD, 77% for N(total) and 94% for the TKN elimination. The ORP in the effluent of the reed bed showed a clear dependence in its characteristic course and its absolute values on the current nitrification performance, the oxygen supply and the hydraulic behaviour of the reed bed. Therefore the ORP is a very good indicator for the state of the reed bed, which ultimately results from the accumulation of a large number of different influencing parameters. As the preservation of aerobic conditions in the reed bed is the crucial prerequisite for a high nitrification performance and for the avoidance of clogging, the ORP thus offers the possibility of immediate operation control.

Ecosystem↗

[Effect and mechanism of removal of nitrogen in the mimic sewage using bio-zeolite system].

On the spot, the removal effect of pollutants such as NH3-N, N, NO3(-)-N, NO2-(-)N, TN and COD in the mimic sewage by bio-zeolite system was studied. The following results were obtained: it was obvious and stable for the removal of NH3-N by bio-zeolite with a removal efficiency over 95%, but the removal effect of NO3(-)-N was affected deeply by hydraulic retention time, according to the different removal mechanism; chemical adsorption and ion exchange were the main reasons for the removal of NH3-N as well as nitrification by microbe, while denitrification was the main reason for the removal of NO3(-)-N. Nitrification intensity of bio-zeolite was affected by the concentration of dissolved oxygen in the solution, so the nitrification intensity of bio-zeolite on the surface of the zeolite bed was two times of that in the middle in vertical direction. Denitrification intensity changed with different values of C/N in the study, and it could reach the maximum while COD/TN was 5; as for denitrification velocity, it was largest in the first six hour.

Adsorption↗

Comparison of intermittently aerated continuous and batch biofilm reactor in nutrient removal.

Removal efficiency of TOC ranged between 86-89% in an intermittently aerated reactor. High efficiency in TKN removal and nitrification was found at lower applied load or longer retention time such as 2 days. TKN removal and nitrification efficiency was found to be 17-96% and 35-99% respectively. Through examination and comparison of the removal efficiencies, the stability of nitrification/denitrification and the biological phosphorus removal it was found that the sequencing batch feeding system gave a higher performance in total nitrogen and phosphorus removals. In the SBR reactor, nitrogen removal efficiency was mainly controlled by organic loading. Nitrification efficiency ranged between 31-56%. Nearly complete denitrification was observed in the sequencing batch reactor.

Biofilms↗

Influence of packing media on nitrogen removal in a subsurface infiltration system.

Influence of packing media on nitrogen removal in a subsurface infiltration system was studied. System A was filled with loamy soil and system B was filled with mixed soil of 75% red clay with 25% cinder. Both systems were fed with sewage at the same hydraulic loading of 2 cm/d at continuous operation mode. The same excellent removal performances of COD and T-P could be achieved in both infiltration systems with removal rates about 85% and 98%, respectively. In system A, NH4+ -N removal rate was as high as 96.5% and T-N removal rate was relatively much lower as 55.7%. And in system B, NH4+ -N removal rate was as low as 75.4% and T-N removal rate was relatively much higher as 75.5% . The difference was attributed to different soil oxidation-reduction condition that was greatly influenced by soil texture in subsurface infiltration system. Loamy soil led to oxidative condition that was favorable to nitrification and disadvantageous to denitrification. The results were just adverse to the system filled with clay. Intermittent operation was adopted to improve nitrogen removal in system B. NH4+ -N removal rate could be increased to about 95% and T-N removal rate could be increased to about 90% at intermittent operation mode in system B. Analysis of nitrogen removal mechanisms indicated that nitrification-denitrification was the primary nitrogen removal path in subsurface infiltration system and crop uptake was another important nitrogen removal way. It was the key to improve the total N removal performance that a suitable packing soil was available to present favorable oxidation-reduction condition for simultaneous nitrification and denitrification.

Aluminum Silicates↗

High strength nitrogen removal from nightsoil and piggery wastes.

Nightsoil and piggery wastes generally present high strength organics and nitrogen. This study evaluated the nitrogen removal characteristics with the existing and modified nightsoil and piggery waste treatment plants. The existing conventional plants showed 20 to 40% nitrogen removal, but the modification with SBR or MLE process could remove effectively both nitrogen and organics with the minimum COD/TN and alkalinity/TN ratios of 6 and 3.6, respectively. Nitrite nitrification and denitrification rates obtainable at higher nitrogen loads were faster than the rates of nitrate nitrification and denitrification resulting in less reactor volume requirement. However, the higher nitrogen loads increased the organic loads resulting in the reactor temperature inhibiting nitrification. Thus, a combined treatment with anaerobic digestion with the adjustment of influent bypass rates was proposed to reduce the reactor temperature and the external carbon requirement. The biological treatment could discharge about 1,100 mg/L soluble COD and 50 mg/L soluble nitrogen, respectively.

Animal Husbandry↗

Nitrogen removal from piggery waste with anaerobic pretreatment.

Aerobic degradation of high strength piggery waste elevated the reactor temperature inhibiting nitrification. This study included anaerobic pretreatment with various influent by-pass rates to control the temperature and to minimize the external carbon requirement for denitrification. To find the optimum operating conditions, both lab-scale AnSBR (anaerobic sequencing batch reactor) and Ax/Ox (anoxic/oxic) SBR were operated at 35 degrees C. The heat energy released from Ax/Ox SBR was assumed to be used for heating the AnSBR, with which the Ax/Ox reactor temperature could successfully be controlled below 40 degrees C. The optimum rates of by-pass were 1.0 for winter, 0.4 for spring/fall and 0.2-0.4 for summer, respectively. Applying the correction factors for the measured AUR2 (nitrite nitrification rate) and AUR (nitrate nitrification) at the predicted temperatures, the required oxic HRTs were computed. The required Ax/Ox HRT ratios were respectively 0.5 for COD/TKN>8, 1.0 for COD/TKN ratio of 5.5-8 and 3.5 for below 5.5. The optimum HRTs were 16 days for AnSBR and 17 days for Ax/Ox SBR with the corrected AUR2.

Agriculture↗

Biological nitrogen removal using bio-sorbed internal organic carbon from piggery wastewater in a post-denitrification MLE process.

Nitrogen removal from a piggery wastewater was investigated in a post-denitrification modified Lüdzack Ettinger (PDMLE) process. Overall hydraulic retention time (HRT) of the PDMLE, consisting of contact/separator (C/S), nitrification, denitrification and re-aerobic bioreactor was 10 days. 60% of the influent SCOD was separated in the C/S by contacting the return sludge with the synthetic wastewater, however, only 10% of the influent SCOD was separated from the piggery wastewater. Biosorption capacities of the synthetic wastewater and piggery wastewater were 800 and 150 mg/g-MLSS, respectively. In spite of the high organic and nitrogen load, nitrification efficiency was above 95%, and nitrification rate was about 180 mg-NH4+-N/L x day. The removed delta COD/delta nitrate ratios in the denitrification tank were 4.0 and 11.5 g-SCOD/g-nitrate, while denitrification rates were 8.4 and 2.6 mg-nitrate/day for synthetic and piggery wastewater, respectively. In the proposed PDMLE process, both bio-sorbed and bypassed organic matter could be successfully used for nitrate reduction as carbon sources and the final TN removal efficiency was as high as 95%.

Adsorption↗

Trickling filters for upgrading low technology wastewater plants for nitrogen removal.

Previous work through the 1990s in the Thames Water region in the UK has demonstrated the ability of the trickling filter process to produce fully nitrified effluents, reliably throughout the year. The original data used for the nitrification model derivations have been reanalysed, to investigate the degree of nitrogen removal across the process. Removals of total nitrogen ranging from 0% to over 50% were observed across the trickling filter process and calculated total nitrogen removals of 26-63% were obtained when primary treatment was included. The degree of nitrogen removal and biological denitrification (excluding cellular assimilation) was found to be strongly influenced by BOD load, irrigation velocity and media size. Regression models were produced which gave good predictive relationships for the data ranges used. The models produced worked for filters used with and without a recirculation of effluent nitrate which suggests that a significant degree of nitrification occurred in areas of high heterotroph activity (BOD removal). The simplicity and energy efficiency of the trickling filter process, combined with its capacity for full nitrification and partial denitrification, make the process attractive as a combined process used with pond systems in developing countries where nitrogen removal may be required. Some of these synergies have already been developed with the PETRO process in South Africa.

Filtration↗

Enhancement of biofilm formation onto surface-modified hollow-fiber membranes and its application to a membrane-aerated biofilm reactor.

Surface-modified hollow-fiber membranes were prepared by radiation-induced grafting of an epoxy-group-containing monomer, glycidylmethacrylate (GMA), onto a polyethylene-based fiber (PE-fiber). The epoxy ring of GMA was opened by introduction of diethylamine (DEA). The bacterial adhesivity to this material (DEA-fiber) was tested by immersion into a nitrifying bacterial suspension. The initial adhesion rates and the amount of attached bacteria of the DEA-fiber were 6-10-fold and 3-fold greater than those of the PE fiber, respectively. A membrane-aerated biofilm reactor (MABR) composed of DEA fibers was developed for partial nitrification with nitrite accumulation. Prior to the nitrification test, it was confirmed that the oxygen supply rate (OSR) was proportional to air pressure up to 100 kPa, allowing easy control of oxygen supply. Stable nitrite accumulation was observed in the partial nitrification test at a fixed oxygen supply throughout the operation period, indicating that oxygen was consumed only by ammonia oxidizers. Furthermore, it was demonstrated that oxygen utilization efficiency (OUE) in the ammonia oxidation process was nearly 100% after 300 h incubation.

Ammonia↗

Water reuse for irrigation from waste water treatment plants with seasonal varied operation modes.

Irrigation periods are usually limited to vegetation periods. The quality requirements for treated wastewater for disposal and for reuse are different. The reuse of water for irrigation allows partly the reuse of the wastewater's nutrients (N and P). Outside the irrigation period the water must be treated for disposal, thus nutrient removal is often required in order to avoid detrimental effects on the receiving surface water body. Only wastewater treatment plants with different operation modes for different seasons can realise these requirements. The nitrification is the most sensitive biological process in the aerobic wastewater treatment process. At low water temperatures the nitrifying bacteria need several weeks to re-start full nitrification after periods without NH4-removal. Therefore it is necessary to develop options for waste water treatment plants which allow a fast re-start of the nitrification process. Based on theoretical considerations and computer simulations of the activated sludge treatment process, one possibility for implementing a wastewater treatment plant with different seasonal operation modes is evaluated.

Agriculture↗

Nitrogen removal during leachate treatment: comparison of simple and sophisticated systems.

Membrane bioreactors (MBR) have become common in treating municipal wastewaters. Applied to leachates treatment MBR were also successful with pilot scale experiments and full-scale facilities as well. We succeeded previously in designing an efficient nitrification-denitrification process with an ethylene glycol byproduct as carbon source for denitrification. Moreover, an unexpectedly high inert COD removal efficiency was also observed in the full-scale MBR facility thereby making it possible to increase the operating time of the final GAC (Granulated Activated Carbon) adsorber. Since MBR are very sophisticated systems. Simpler and "lower" cost systems can also be considered. For example it is possible to nitrify leachates from sanitary landfill using a simple infiltration-percolation technique with a low energy cost. To validate previously published laboratory experiments, a semi industrial-scale pilot installation was installed at the Montzen landfill site (Belgium). The process is based on infiltration-percolation through a granular bed. This well known process was modified to increase the load, notably by changing the support medium, adding an electric fan that is run intermittently and maintaining temperatures greater than 15 degrees C. The new material is a type of granular calcium carbonate with a large specific surface area. These technical improvements enabled the system to nitrify up to 0.4 kg NH4+-N/m3 of reactor bed per day at a hydraulic load of 0.35 m.d(-1), with an ammonia removal rate in the range of 80 to 95%. Despite the high ammonia nitrogen inlet concentrations, this system exhibits remarkable nitrification efficiency. Moreover, these performances are achieved in a batch mode system without recirculation or dilution processes. If complete nitrification is needed, it can be obtained in a second in series of bioreactors. The system can be classified as a low cost process. An international patent is pending. Possible performances of those systems were compared with the usual methods for leachates treatment.

Ammonia↗

Application of a sponge media (BioCube) process for upgrading and expansion of existing caprolactam wastewater treatment plant for nitrogen removal.

For the upgrade and expansion of an existing caprolactam wastewater treatment plant, a freely floating sponge media (BioCube) process was selected based on extensive pilot-plant tests, due to extreme space constraints. In order to protect nitrifier inhibition caused by high strength organics in caprolactam wastewater, the pilot plant consisted of an organics removal reactor, which functioned as a pretreatment for nitrification, and followed the nitrogen removal reactor. The suspended MLSS was 1,800-4,000 and the media attached MLSS was maintained at 22,000-26,000 mg/L. The final effluent COD was noticeably low, around 20.4-37 mg/L, even with fairly large fluctuations in the feed levels, between 1,400-6,770 mg/L. The removal of total nitrogen with the system, when denitrification was close to completion, was approximately 97.6%. For the entire run, complete nitrification of 99.6% was achieved, which might have been due to well-acclimatized nitrifiers attached in the BioCube media. Specifically, after adaptation, the nitrification continuously increased in the organics removal reactor, even under high residual organics conditions. From the numerous experimental results, the BioCube process seemed to be an effective method for the upgrading and expansion of the existing wastewater treatment plant, with minimum reactor enlargement.

Animals↗