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At least 631 records · Page 35Linked to original sources
PubMed1987

Splenic function in haemophilia.

Studies of splenic function were carried out on 17 haemophilic patients over 9 years of age, and 20 control patients. The clearance of autologous heat-damaged 99mTc-labelled erythrocytes from circulation and into the spleen was measured: the spleen area by scintillation scanning, and the enumeration of pitted erythrocytes by direct interference microscopy. Splenic enlargement was observed in 10 patients (59%). On the basis of the clearance half-time, splenic function was normal in 3/13 (23%) and hyperactive in 9/13 (69%) patients. One heavily transfused patient had a hypoactive spleen with long clearance half-time, slow splenic uptake of radioactivity and high pit counts. These results demonstrate that the spleen of haemophiliacs is usually enlarged and functionally abnormal.

Adolescent↗
PubMed1985

Isolated follicular dendritic cells: cytochemical antigen localization, Nomarski, SEM, and TEM morphology.

The objectives of the present study were to determine the cytological features of isolated follicular dendritic cells (FDC), which distinguish them from other leukocytes or dendritic cell types. Consequently, we have developed methods for the fixation, peroxidase cytochemistry, and visualization of FDC, which are applicable to cytological evaluations by Nomarski optics, scanning, and transmission electron microscopy. A functionally supported identification of FDC in vitro was made possible by utilizing, in conjunction with the dendritic morphology, the cytochemically identifiable antigen, horseradish peroxidase (HRP), and the known capacity of FDC to sequester immune complexes (i.e. HRP-anti-HRP) on their plasma membranes. The observations showed that FDC constitute a relatively pleomorphic, nonphagocytic group, distinct from other dendritic type cells such as lymphoid dendritic cells, Langerhans cells, and interdigitating cells (LDC, LC, and IDC), as well as typical leukocytes. Morphologically distinct FDC were identified as cells either with filiform dendrites or with "beaded" dendrites. FDC possessed a single or sometimes a double, lymphocyte-size cell body, which contained an irregular, lobated nucleus, Golgi apparatus, numerous small vesicles, and some mitochondria. Mitochondria were not abundant in the dendritic processes. Filiform dendrites tended to branch and anastomose near the cell body and form a radiating "sunburst"-like pattern. On the average, dendrites measured 15-20 microns in length and 0.1-0.3 micron in diameter. Occasional dendrites were extremely elongated, reached several hundred microns in length, and terminated in an enlargement measuring nearly a micron in diameter. Other filiform dendrites usually had a club-shaped terminal enlargement. The microspheres of "beaded" dendrites ranged between 0.3 and 0.6 micron in diameter. The dendritic processes were also shown to have a highly ordered pattern of immune complex attachment on their surface, suggestive of a periodic arrangement of receptor sites.

Animals↗
PubMed1985

The coordinate organization of vinculin and of actin filaments during the early stages of fibroblast spreading on a substratum.

Cultured normal fibroblasts adhere to their support essentially through the focal adhesion plaques which are greatly enriched with the 130 000 dalton protein, vinculin, along with the newly described 215 000 dalton protein, talin, and at which actin bundles terminate. In order to explore a role for vinculin in the formation of the adhesion plaques and of the actin bundles, we have studied and compared the development of these two cellular structures during the spreading of trypsinized and replated chicken embryonic fibroblasts. The techniques used were double indirect immunofluorescence and interference reflection microscopy. At the earliest stage of cell spreading observed, vinculin distributes into small patches that are located along actin filaments and at the basis of the ruffling membrane. At later spreading stage, vinculin markedly redistributes into larger striations which coincide with focal contacts. Some of these vinculin striations are associated with the ends of microfilaments while the others are not. These observations would suggest that two types of focal contacts can form simultaneously in early cell spreading. Hypotheses are made concerning the role of vinculin in the formation of the adhesive cell structures in the light of these new data and of previous reports on the subject.

Actins↗
PubMed1985

[Quantitative DNA determination and its role in the prognosis of malignant tumors].

We summarize our own cytophotometric and flow cytometric measurements from malignant tumors and premalignant lesions. DNA, non-histoprotein and dry mass (by interference microscopy) have been assessed. Ploidy and the compartments of the cell cycle were evaluated. For most tumors (cervix, breast, ovary, melanoma, larynx, oro-facial, bladder) a bad prognosis was suggested by the following cytophotometric findings: aneuploidy, polyploidy, and/or a high proportion of S-G2/M nuclei. This has been statistically confirmed for breast cancer by case studies conducted over a period of five years. Among the changes associated with lung cancer were alterations in protein content. Cytophotometric findings can provide useful additional information concerning a patient's prognosis.

Cell Division↗