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625 records · Page 35Linked to original sources
PubMed1978

[Role of cobalt in the biosynthesis of the components of the gentamicin complex].

It was shown that addition of cobalt ions or vitamin B12 to the fermentation medium resulted in an increase in the level of gentamicin accumulation, the relative content of the most methylated components C1 and C2 in the gentamicin complex being increased, while the content of the least methylated components CIa and "minors" decreased. Addition of sulphodimezine lowered the gentamicin biosynthesis rate and the relative content of gentamicins C1 and C2. It was supposed that cobalt stimulated the B12-dependent synthesis of methionine, being the source of the methyl groups for biosynthesis of the methylated components of gentamicin complex.

Cobalt↗
PubMed1981

[Development of a gentamicin producer under different culture conditions studied by a method of differential centrifugation of the mycelium in a saccharose density gradient].

The method of differential centrifugation in the sucrose density gradient (SDG) enabled one to trace the changes in the development of the seed and fermentation mycelium of the gentamicin-producing organism. Correlation between gentamicin distribution in the SDG and the culture productivity was found. It was shown that the culture grown under the optimal aeration and agitation conditions was characterized by formation of higher amounts of the mycelium in the 5th and 6th layers of the SDG. Such mycelium was more productive than that from the other SDG layers. The most productive 48-hour seed culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture was used as the seed material, the activity of the fermentation broth was the highest. The method of differential centrifugation in the SDG provides determination of the culture productivity by the volumes of the fermentation mycelium in the 5th and 6th layers or the seed mycelium in the 3rd layer of the SDG.

Centrifugation, Density Gradient↗
PubMed1981

[Significance of pH for the growth of the sisomicin producer and biosynthesis of the antibiotic].

Dependence of the mycelium growth and sisomycin production on the pH values of the fermentation broth was studied in cultures of various ages for 6 hours with the method of pH control within the preset levels. It was shown that the values of pH within the range of 7.25 - 7.6 were optimal. The pH values of 7.5 - 8.0 and 7.0 - 8.0 were optimal for the growth of the 24-hour culture and biosynthesis of the antibiotic by it respectively. pH 7.5 was optimal for the growth of the 48-hour mycelium and biosynthesis of the antibiotic by it.

Culture Media↗
PubMed1981

[Oxidative-reductive activity of the mycelium as a criterion for assessing inoculate quality in gentamycin biosynthesis].

The development of the seed mycelium at various stages and temperatures was studied with the method of methylene blue decolorization characterizing the respiration level of the culture and its oxidation-reduction (dehydrogenase) activity. Relationship between the time of methylene blue decolorization by the seed mycelium, the quantity of the inoculum transferred from one stage to the other and the temperature of cultivation was shown. The seed mycelium decolorizing methylene blue within 2 minutes was most productive at the biosynthetic stage irrespective of the growth period, biomass volume, cultivation temperature and volume of the inoculum transferred from one stage to the other.

Culture Media↗
PubMed1981

[Effect of aeration and agitation conditions on gentamycin biosynthesis].

The study on the relation between the production of gentamicin and the conditions of aeration and agitation of the fermentation broth showed the advisability of the control of these parameters with respect to the maximum rate of the oxygen uptake. Possible scaling-up of the process of gentamicin biosynthesis was shown with the use of the expression: (formula: see text).

Aerobiosis↗
PubMedDate not supplied

[Degradation of cellulose by micromonospores].

Sodium benzoate is suggested as an inhibitor of cellulose decomposing bacteria and fungi for detecting and counting micromonospores. Cellulose decomposing micromonospores were found in the zone adjacent to the roots of winter wheat and maize; their number, cellulose decomposing activity, morphological, physiological, and antagonistic properties were determined. Pasteurization of the material taken from colonies grown on cellulose is recommended for isolation of pure cultures.

Benzoates↗