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PubMed2005

ATR-FTIR membrane-based sensor for the simultaneous determination of surfactant and oil total indices in industrial degreasing baths.

Monitoring the exhaustion of alkaline degreasing baths is one of the main aspects in metal mechanizing industrial process control. The global level of surfactant, and mainly grease, can be used as ageing indicators. In this paper, an attenuated total reflection-Fourier transform infrared (ATR-FTIR) membrane-based sensor is presented for the determination of these parameters. The system is based on a micro-liquid-liquid extraction of the analytes through a polymeric membrane from the aqueous to the organic solvent layer which is in close contact with the internal reflection element and continuously monitored. Samples are automatically processed using a simple, robust sequential injection analysis (SIA) configuration, on-line coupled to the instrument. The global signal obtained for both families of compounds are processed via a multivariate calibration technique (partial least squares, PLS). Excellent correlation was obtained for the values given by the proposed method compared to those of the gravimetric reference one with very low error values for both calibration and validation.

Metallurgy↗
PubMed2006

Reliable and rapid identification of Listeria monocytogenes and Listeria species by artificial neural network-based Fourier transform infrared spectroscopy.

Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spectra remains difficult. Here, we apply artificial neural network technology (ANN), which is an advanced multivariate data-processing method of pattern analysis, to identify Listeria infrared spectra at the species level. A hierarchical classification system based on ANN analysis for Listeria FTIR spectra was created, based on a comprehensive reference spectral database including 243 well-defined reference strains of Listeria monocytogenes, L. innocua, L. ivanovii, L. seeligeri, and L. welshimeri. In parallel, a univariate FTIR identification model was developed. To evaluate the potentials of these models, a set of 277 isolates of diverse geographical origins, but not included in the reference database, were assembled and used as an independent external validation for species discrimination. Univariate FTIR analysis allowed the correct identification of 85.2% of all strains and of 93% of the L. monocytogenes strains. ANN-based analysis enhanced differentiation success to 96% for all Listeria species, including a success rate of 99.2% for correct L. monocytogenes identification. The identity of the 277-strain test set was also determined with the standard phenotypical API Listeria system. This kit was able to identify 88% of the test isolates and 93% of L. monocytogenes strains. These results demonstrate the high reliability and strong potential of ANN-based FTIR spectrum analysis for identification of the five Listeria species under investigation. Starting from a pure culture, this technique allows the cost-efficient and rapid identification of Listeria species within 25 h and is suitable for use in a routine food microbiological laboratory.

Bacteriological Techniques↗
PubMed1999

Applications of ion chromatography in the semiconductor industry. II. Determination of basic airborne contaminants in a cleanroom.

Since the geometry of semiconductors and integrated circuits has been shrunk to well below sub-micron dimensions, there is a great demand for precise and reliable analytical techniques to measure and monitor the contaminants in all areas related to the fabrication process. Special concerns about the air cleanliness in a Fab lead to the necessity for developing analytical techniques to perform this task. In this research, basic airborne contaminants in a cleanroom were adsorbed onto a collection tube, subsequently extracted with deionized water and analyzed by ion chromatography. Such a method is capable of simultaneously measuring the concentrations of ammonia and cations (e.g., sodium, potassium, calcium, magnesium, etc.) present in the cleanroom air samples. The optimal sample preparation method was determined and the analyte concentrations at various locations in the cleanroom were measured. The results showed significant variations from one location to another. The long-term fluctuations in the contaminant levels were also significant. Data obtained using this method compared well with data from inductively coupled plasma analysis of the same materials.

Air Pollutants↗
PubMed1992

Modeling human operators using neural networks.

Often in the process industries, human operators rather than mathematically based advanced algorithms are used to achieve product control. Typically, if there are three shifts of operators per day, one shift achieves superior control. If the expertise of the best operator can be captured easily and economically and made available to the other operators, significant economic benefits would accrue. This paper discusses a methodology that uses artificial neural networks for capturing the knowledge of process operators. For many operator tasks, only readily available information obtained from a process control computer is required. Once a converged network is available, a stripping technique can be employed to simplify the net and to gain knowledge about what a good operator is doing compared to a poor one. It is felt that the proposed approach is superior to traditional expert system techniques employing knowledge extraction approaches.

Humans↗
PubMed2006

Effects of nutrient addition on phenol biodegradation rate in biofilm reactors for hypersaline wastewater treatment.

Hypersaline wastewater, generated by many industrial activities, is difficult to treat through conventional biological processes. In this kind of hypersaline environment, complex nutrients are needed for the normal growth of many microorganisms. For this paper, the organisms were taken from a municipal wastewater treatment plant and acclimated to 15% salinity in a biofilm treatment process (Sequencing Batch Biofilm Reactor) during the treatment of phenol-containing synthetic wastewater. They are used to evaluate the effects of nutrient addition on the phenol biodegradation rate. Adding yeast extract, glucose, KCl and four mineral nutrients into the shaking flasks containing wastewater and cultivating organisms, revealed phosphate as the crucial nutrient stimulating phenol biodegradation at 15% salinity. The operation results of the sequencing batch biofilm reactor indicates that phosphate content increased up to five times the original level can increase the phenol removal rate by 150%. A 99% phenol removal efficiency could be achieved by shortening the reaction time in the biofilm reactor from 40 h to 16 h, compared with basic nutrients added. In this article we applied an applicable and effective shaking-culture method to determine nutrient requirements in biotreatment processes without stopping the running reactors.

Biodegradation, Environmental↗
PubMed1981

Animal wastes. I. Effect of processing on the chemical and protein nutritional value of calf stomach.

The nutritive value of calf stomach waste from rennet production was assessed. The by-product was either drum-dried at the industry (DDSW) or mildly dried (60 degrees C) in a ventilated oven at the laboratory (MDSW). Part of the sample treated at the laboratory was autoclaved before drying (ASW). The protein and ether extract concentrations ranged from 34.25% to 44.31% and from 31.14% to 38.95%, respectively, and were lower in DDSW than in the other two samples. The ash content which ranged from 16.04% to 27.38%, was higher in the DDSW sample. Methionine, cystine, and available lysine were lower in the DDSW by-product than in the other two samples. Lysine availability was affected by the drum-drying and autoclaving procedures. Tryptophan was shown to be the limiting amino acid in all samples. Nutritive value, assayed both in DDSW and ASW, was lower in DDSW when compared with ASW or with casein diets as far as PER is concerned. The digestibility was shown to be ranked in increasing order for DDSW, ASW and casein. In conclusion, the results indicate that the waste from rennet production may be recommended as in inexpensive source of protein for animal feeding.

Amino Acids↗
PubMed1996

Fermentation of orange peel hydrolysates by ethanologenic Escherichia coli. Effects of nutritional supplements.

Orange peel, an abundant byproduct of the citrus processing industry, is converted to a mixture of glucose, galacturonic acid, fructose, arabinose, galactose, and xylose by hydrolysis with mixed pectinase and cellulase enzymes. All these sugars can be fermented to ethanol or ethanol and acetic acid by the recombinant bacterium Escherichia coli KO11. The fermentation efficiency is improved by the addition of yeast extract, tryptone, mixed amino acids, corn steep liquor, or by proteolytic digestion of endogenous proteins. Batch fermentations of supplemented peel hydrolysate containing 111 g/L of initial total sugars produced 35-38 g/L of ethanol in 48-72 h and a 75-85% yield.

Citrus↗
PubMed2004

Mutagenic activity of sweepings and pigments from a household-wax factory assayed with Salmonella typhimurium.

The mutagenic activity of garbage originating from a household wax industry was determined by the Salmonella/microsome assay, using the bacterial strains TA100, TA98 and YG1024. The garbage was obtained by sweeping the floor of the factory at the end of the work shift. Organic compounds were extracted by ultrasound for 30 min in dichloromethane or 70% ethanol. After evaporation of solvent, these extracts (HFS: household-wax factory sweepings) were dissolved in DMSO, and were tested for the mutagenic activity at varying concentrations (HFS-ET: 0.08-0.68 mg/plate, HFS-DCM: 0.60-7.31 mg/plate). The colouring agents (pigments) used in the production of the wax were also dissolved in DMSO and tested with the assay. The concentrations tested for each pigment were: Amaranth: 0.46-3.65 mg/plate, Auramine: 0.15-1.2 mg/plate and Rhodamine B: 0.22-1.82 mg/plate. Both ET and DCM organic extracts had mutagenic activity, especially in the YG1024 strain. The pigments behaved in a similar way, demonstrating that YG1024 was the most sensitive strain for the detection of mutagenicity, and that metabolization increased the activity. Human exposure (occupational and non-occupational) to industrial residues generated during the household-wax manufacturing and packaging process should be monitored, since this type of garbage is normally deposited in the environment without any control.

Animals↗
PubMed1990

Image processing by computer analysis--potential use and application in civil and criminal litigation.

The image processing by computer analysis has established a data base for applications in the industrial world. Testing has proved that the same system can provide documentation and evidence in all facets of modern day life. The medicolegal aspects in civil and criminal litigation are no exception. The primary function of the image processing system is to derive all of the information available from the image being processed. The process will extract this information in an unbiased manner, based solely on the physics of reflected light energy. The computer will analyze this information and present it in pictorial form, with mathematical data to support the form presented. This information can be presented in the courtroom with full credibility as an unbiased, reliable witness. New scientific techniques shown in the courtroom are subject to their validity being proven. Past imaging techniques shown in the courtroom have made the conventional rules of evidence more difficult because of the different informational content and format required for presentation of these data. I believe the manner in which the evidence can now be presented in pictorial form will simplify the acceptance. Everyone, including the layman, the judge, and the jury, will be able to identify and understand the implications of the before and after changes to the image being presented. In this article, I have mentioned just some of the ways in which image processing by computer analysis can be useful in civil and criminal litigation areas: existing photographic evidence; forensic reconstruction; correlation of effect evidence with cause of evidence; medical records as legal protection; providing evidence of circumstance of death; child abuse, with tracking over time to prevent death; investigation of operating room associated deaths; detection of blood at the scene of the crime and on suspected objects; use of scales at the scene of the crime; providing medicolegal evidence beyond today's technology; and a new theory and technique on using polygraph information in litigation. I am sure that the professionals in the forensic field will be able to think of many more applications where the image processing by computer analysis tool will be able to provide solutions to complex problems. The next time you say to yourself, "I wish they would have preserved this," or, "It's too bad they didn't do an autopsy," think of this new tool that is available to help you get the documentation and answers that will stand up to the scrutiny of the civil and criminal litigation system.

Accidents, Traffic↗
PubMed2005

Possibilities for transfer of relevant data without revealing structural information.

In this paper, we discuss how we safely exchanged proprietary data between third parties in the early years of predictive ADME/Tox model development. At that time, industry scientists wanted to evaluate predictive models, but were not willing to share their structures with software vendors. At the same time, model developers were willing to run the scientists' structures through their models, but they were not willing to reveal which descriptors were important for a particular predictive model. We developed a process where scientists could perform calculations on a broad number of commercially available public descriptors and forward results as a property file, instead of their structures. Meanwhile, the model developer could extract descriptors used in the predictive model, run the model, and pass results back to the scientist. On the following pages, we discuss the pros and cons of this approach, and we address questions such as: Can structural information that is proprietary be compromised from descriptors in ADME/Tox models? Can ADME/Tox predictions be made purely from descriptors, without the explicit knowledge of chemical structures, proprietary or otherwise?

Chemistry, Pharmaceutical↗
PubMed1986

New aspects on the chemistry of tea and coffee. III.

The previous review for 1983/1984 is now continued with references to recent publications (1984/1985) and as before covers the chemistry and technology of tea (leaf, green and black) and coffee (raw, roasted and instant soluble), with particular reference to composition and changes during manufacture, extraction and storage. The effects of composition on the quality of the beverage, with mention of particular physiological properties, are included (210 references).

Chemical Phenomena↗
PubMedDate not supplied

Raspberry ketone from submerged cultured cells of the basidiomycete Nidula niveo-tomentosa.

The basidiomycete Nidula niveo-tomentosa produced 4-(4-hydroxyphenyl)-butan-2-one (raspberry ketone), one of the character impact components of raspberry flavor, and its corresponding alcohol. A systematic attempt was made to improve the productivity of this fungus. Variation of nutrient medium composition, precursor amount, time of supplementation, and cultivation period yielded a 50-fold increase in metabolite concentrations. Raspberry ketone and alcohol were easily isolated from the culture medium by solvent extraction. Glycosidically bound forms or accumulation of raspberry compounds in fungal cells were not detected. This microbial process offers an alternative for the production of natural raspberry flavor.

Basidiomycota↗
PubMed2006

The mutagenic potential of madder root in dyeing processes in the textile industry.

The roots of Rubia tinctorum L. have a long tradition of being used in dyeing processes of textiles from centuries ago until the present time. The colouring principles belong to the class of hydroxyanthraquinones. Concern arose because several of these compounds were recognised as mutagenic in vitro and even carcinogenic in rodents. To assess the possible risk to humans caused by coloured textiles, mutagenicity was investigated with two madder root samples of different origin (Iran and Bhutan) along the entire dyeing process from root extracts to the dyed wool. The Salmonella/microsome test (Ames assay) with the strains TA98, TA100 and TA1537 was used. Significant mutagenic effects could be detected in madder root extracts and also in the final product, the dyed wool. Madder root from Iran showed considerably higher mutagenic responses than samples from Bhutan. Analytical investigations of the extracts by HPLC showed the presence of a spectrum of anthraquinones typical for madder root. Three mutagenic compounds, lucidine, rubiadine and purpuroxanthine, together with the non-mutagenic alizarine could be detected. The mutagenic response of the different samples was positively correlated with the concentration of the mutagenic anthraquinones, and with lucidine in particular. Based on these investigations a risk to dye-house workers and users of textiles dyed with R. tinctorum must be anticipated.

Animals↗
PubMed1999

Biomass conversion to mixed alcohol fuels using the MixAlco process.

The MixAlco process is a patented technology that converts any biodegradable material (e.g., sorted municipal solid waste, sewage sludge, industrial biosludge, manure, agricultural residues, energy crops) into mixed alcohol fuels containing predominantly 2-propanol, but also higher alcohols up to 7-tridecanol. The feedstock is treated with lime to increase its digestibility. Then, it is fed to a fermentor in which a mixed culture of acid-forming microorganisms produces carboxylic acids. Calcium carbonate is added to the fermentor to neutralize the acids to their corresponding carboxylate salt. The dilute (approximately 3%) carboxylate salts are concentrated to 19% using an amine solvent that selectively extracts water. Drying is completed using multi-effect evaporators. Finally, the dry salts are thermally converted to ketones which subsequently are hydrogenated to alcohols. All the steps in the MixAlco process have been proven at the laboratory scale. A techno-economic model of the process indicates that with the tipping fees available in New York (126 dollars/dry tonne), mixed alcohol fuels may be sold for 0.04 dollars/L (0.16 dollars/gal) with a 60% return on investment (ROI). With the average tipping fee in the United States rates (63 dollars/dry tonne), mixed alcohol fuels may be sold for 0.18 dollars/L (0.69 dollars/gal) with a 15% ROI. In the case of sugarcane bagasse, which may be obtained for about 26 dollars/dry ton, mixed alcohol fuels may be sold for 0.29 dollars/L (1.09 dollars/gal) with a 15% ROI.

Journal Article↗
PubMed2003

Fate of the fungicide dodemorph during anaerobic digestion of biological waste.

The aim of this study was to examine the stability and distribution of the fungicide dodemorph during anaerobic digestion of biological waste, including a short subsequent aerobic treatment. Three reactor experiments were conducted. The influences of pH and dissolved organic carbon (DOC) were further studied in batch experiments. The reactor experiments showed no decrease in the concentration of dodemorph, indicating stability of the fungicide. In connection with the results of the batch experiments, a clear pH-dependency of the partitioning of dodemorph between solid and water phase could be shown. At pH values below the pK(a) value of 7.8, protonated dodemorph molecules predominate. However, a release into the water phase was only observed at pH values of 5 and below. It can be assumed that in the pH range between 5 and 7.8 dodemorph forms bonds with carboxyl groups of organic matter. Dodemorph was not completely extractable from the solid waste matrix due to strong bonds with the organic matter. Solubilising effects by water ingredients of the process water were not apparent.

Anaerobiosis↗
PubMed2003

Simplified modeling of fed-batch alcoholic fermentation of sugarcane blackstrap molasses.

Simplified modeling based on material balances for biomass, ethanol and substrate was used to describe the kinetics of fed-batch alcohol fermentation of sugarcane blackstrap molasses. Maintenance requirements were previously shown to be of particular significance in this system, owing to the use of massive inoculum to minimize inhibitions; therefore, they were taken into consideration for kinetic modeling. Average values of biomass and ethanol yields, productivities, and substrate consumption rates, calculated at the end of runs performed either at constant or exponentially varying flow rates, demonstrated that all of these parameters were influenced by the initial sugar-feeding rate, F(o)S(o). Under conditions of substrate shortage (F(o)S(o) </= 300 g(S) h(-1)), the amount of carbon dioxide produced was higher than that corresponding to the stoichiometry of sucrose fermentation to ethanol, indicating that an appreciable fraction of the carbon source was likely consumed by respiration. Besides, the biomass yields either on substrate, Y(X/S), or ethanol, Y(X/E), as well as the product yield on substrate, Y(E/S), notably decreased. These results are in agreement with the relatively high specific rate of anaerobic substrate consumption for maintenance estimated for this system (m(a) (s) = 0.789 g(S) g(X) (-1) h(-1)), which was responsible for the consumption of more than 70% of the fed carbon source. The proposed equations derived from the Monod model proved to be a useful tool to easily predict the performance of this process.

Bioreactors↗
PubMed2004

Identification of virgin olive oil from different cultivars by analysis of DNA microsatellites.

DNA analysis enables genome fingerprinting with consequent identification of different individuals. In the agro-food industry, this can have interesting applications for the identification of species and cultivars of both raw materials and processed food. In this investigation, the efficiency of DNA microsatellite analysis in identifying virgin olive oils from different cultivars was evaluated. Ten virgin oils were obtained in the laboratory from olives of 10 different cultivars and the DNA extracted from the cell residues, recovered by oil centrifugation, was used as a template with seven different primer pairs of microsatellite sequences. The electrophoretic patterns showed an adequate level of amplification and were identical to those obtained from leaves and drupes of the same cultivar. By analyzing all the patterns obtained, the smallest number of microsatellites able to distinguish the examined oils was established and an identification key for the different oils was developed.

DNA, Plant↗
PubMed2004

Phloroglucinol from phlorin hydrolysis for testing quality of commercial orange juices and beverages.

The European Association of Juices and Nectars Producers (AIJN) is evaluating the opportunity to introduce the content of phlorin (3,5-dihydroxyphenyl beta-D-glucopyranoside), a peel marker for oranges, as a parameter for testing the quality of orange juices. Because of the lack of a commercial standard of phlorin and its laborious isolation procedures, in this contribution is developed a simple and reliable method for measuring the phlorin level as the corresponding aglycon phloroglucinol, obtained after a total enzymatic hydrolysis of the sample. The method was applied to the quantification of phloroglucinol in several industrial and commercial blond and pigmented orange juices and beverages based on 12% orange juice. Under the same extraction procedure, the phloroglucinol content in the pigmented juices was higher than in the blond ones. No significant difference was obtained between not from concentrate juices and reconstituted from concentrate juices. The marker amount increases in the highly processed orange fruits and in the byproducts of citrus processing due to the contact of the juice with the albedo, which is the major source of phlorin. In orange-based beverages the phloroglucinol content revealed a large heterogeneity and a poor quality of the raw juices used.

Beverages↗