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Intraventricular infusions of heroin (an opiate) or levorphanol (an opioid) in rats produced rigidity but not explosive motor behavior (EMB). When preceded by an IP injection of naloxone, infusions of heroin but not levorphanol produced EMB. The results suggest that naloxone-antagonized effects of opiates mask EMB and that certain opioids do not induce EMB.
A 19 year old soldier suffered an explosion injury of the face. He lost the left cerebral hemisphere, the left eye and ear and the majority of the facial musculature on the left side. Primary surgery with rotational flap and mesh graft. Further local flaps and revision of wound area followed. Inspite of nearly complete destruction of the left cerebral hemisphere the patient could walk with only slight hemiparesis. He learned to write with his left hand, he could read from the lips and stand. An understandable speech could not be obtained. Fitting of an epithesis.
This paper presents the reconstruction of an unusual case of suicide. After raiding a branch-bank a robber fled shooting with his Sauer-Western revolver caliber .44 magnum at the pursuing policemen and succeeded in wrestling a pistol Walther caliber 7,65 mm from them. Under the fire of sub-machine guns he destroyed himself by a shot to the neck. Our investigations concerned a textile damage at the front of the sweater of the deceased surrounded by primer residue, showing characteristics of a close-up shot. The damage was identified as effect of explosion gases exhausting far-off the muzzle. The distance between this injury and the bullet hole corresponded with the length of the barrel of the Sauer-Western revolver and could be used for identification; it confirmed the diagnosis of a close-up shot at the neck, too. Collateral experiments with shots from distant ranges developed spadiceous melt figures of textile fibers around the bullet hole, the appearance of which is considered proof for a close-up shot commonly.
B2 repeats are a group of short interspersed elements (SINEs) specific for rodent genomes. Copy numbers were determined for different rodent genera. All the Muroid (rat, mouse, deer mouse, hamster, gerbil) rodent genomes analyzed exhibited 80,000-100,000 copies per haploid genome, whereas the squirrel genome contains only 2,500 copies, and fewer than 100 (if any) copies were observed for the Hystricognath rodents (guinea pig and nutria). These findings demonstrate that there was an 'explosion' of amplification of B2 elements within muroid rodents. The similar copy number of B2 elements within the different muroid species could be explained by formation of a high proportion of the B2 elements prior to the divergence of the different muroid species. However, the 3'-end of the B2 sequence is unique between murid and cricetid rodents suggesting that the majority of elements amplified after the divergence of these species. Also consistent with recent amplification of these elements in parallel within the muroid genomes is the finding that within mouse and rat there are distinct subfamilies of B2 repeats. The pattern of consistent parallel amplification of B2 elements in muroid species contrasts with the sporadic nature of ID repeat amplification in the same genomes. The consensus of the young mouse subfamily of elements corresponds to the B2 RNA that is preferentially transcribed in embryonic, tumor, and normal liver cells. The subfamily is young based on both its low divergence from the subfamily consensus sequence and the finding that the most recent B2 element insertions in the mouse genome are members of this subfamily.
A dynamometer for measurement under static and dynamic conditions is presented. At different load levels, force, velocity, work and power can be measured in explosive leg extensions. Measurements on 53 subjects at different load levels (0-125.5 kg) were carried out. Peak power ranged from 2611 to 1746 W, force from 1351 to 1899 N, velocity from 1.61 to 0.89 m X s-1 and work from 329 to 605 J. Between trial correlation coefficients ranged from 0.72 to 0.95. The dynamometer is compared with others, and it is concluded that data obtained by this dynamometer have a greater practical validity.
Dizocilpine (MK-801) administration to an outbred strain of NIH Swiss mice elicits discrete episodes of explosive jumping behavior designated as "popping." This behavior may serve as a useful preclinical paradigm for the screening of potentially novel antipsychotic medications. Both nicotine and mecamylamine, a nicotinic antagonist, dose-dependently blocked dizocilpine-induced popping. The data suggest that nicotine may be of therapeutic benefit in the treatment of schizophrenia and that some of its effects may be mediated by non-nicotinic receptors.
The nuclear receptor superfamily expanded in at least two episodes: one early in metazoan evolution, the second within the vertebrate lineage. An exception to this pattern is the genome of the nematode Caenorhabditis elegans, which encodes more than 270 nuclear receptors, most of them highly divergent. We generated 128 cDNA sequences for 76 C. elegans nuclear receptors, confirming that these are active genes. Among these numerous receptors are 13 orthologues of nuclear receptors found in arthropods and/or vertebrates. We show that the supplementary nuclear receptors (supnrs) originated from an explosive burst of duplications of a unique orphan receptor, HNF4. This origin has specific implications for the role of ligand binding in the function and evolution of the nematode supplementary nuclear receptors. Moreover, the supplementary nuclear receptors include a group of very rapidly evolving genes found primarily on chromosome V. We propose a model of lineage-specific duplications from a chromosome on which duplication and substitution rates are highly increased. Our results provide a framework to study nuclear receptors in nematodes, as well as to consider the functional and evolutionary consequences of lineage-specific duplications.
Soils from the Yorktown Naval Base contaminated with trinitrotoluene (TNT) and other explosives were used to prepare eluates before and after bioremediation using microbial growth amendments in the presence (P1 eluates) or absence (P2 eluates) of exogenous white rot fungus. Effectiveness of bioremediation was examined by several immunotoxicity assays-viability/growth of lymphocytes, cytokine production, and expression of the interleukin-2 (IL-2) receptor-using human peripheral blood mononuclear cells exposed to the eluates. Although TNT concentrations decreased in both P1 and P2 eluates relative to untreated baseline soil (BL) eluates, a recovery in lymphocyte growth/viability and IL-2 secretion was seen with P2 but not P1 eluates relative to BL eluates. IL-2 receptor levels were higher in cells exposed to BL and P2 eluates than when exposed to P1 eluates. Interferon-gamma, tumor necrosis factor-beta, and IL-10 levels were highest in BL and P2 eluates and lowest in P1 eluates. Taken together, these results suggest that treatment of the soil with microbial growth amendments in the absence but not the presence of exogenous white rot fungi lead to partial bioremediation as assessed by lymphocyte functions.
The cellular responses of Pseudomonas sp. HK-6 to explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) have been extensively analyzed in this study. The stress shock proteins, which might contribute to enhancing the cellular resistance to the cytotoxic effect of RDX, were induced at different concentrations of RDX used as a substrate for cell culture of Pseudomonas sp. HK-6. The proteins were identified as 70-kDa DnaK and 60-kDa GroEL by SDS-PAGE and Western blot using the anti-DnaK and anti-GroEL monoclonal antibodies. The stress shock proteins induced by RDX were found to increase in proportion to the RDX concentration used for this work. Analysis of membrane fatty acids of strain HK-6 following exposure to RDX showed that the amounts of dominant lipids 16:1 omega7c/15:0 iso 2OH, 16:0 and 18:1 omega7c/omega9t/omega12t decreased substantially or were not detected in the cells exposed to RDX, while amounts of lipids 10:0 iso, 14:1 omega5c/omega5t and 16:10 methyl increased dramatically. Scanning electron microcopy analyses revealed the presence of perforations and irregular rod shapes with wrinkled surfaces for cells treated with 0.135 mM RDX for 12 h, suggesting that RDX has a substantial cytotoxic impact on cells of strain HK-6.
Two data groups were analyzed: (1) the exposure rate in the former Czechoslovakia after the Chernobyl accident in 1986, and (2) the decrease of beta activity of an atmospheric fallout sample taken in Bratislava during 24 h on 30 May 1965. Both quantities decreased with the first power of time. This pattern of decrease is explained by applying the same mathematical formalism as is also used to describe the decrease in postnatal mortality with age. Following this formalism, the decrease of total activity with the first power of time could be seen as a consequence of a log-normal distribution of decay constants in the fallout. This differs slightly from earlier results that show the total activity decreasing with a power of 1.2 immediately after the nuclear explosion.
Maximal explosive power during two-leg jumps was measured on four sedentary subjects [mean age 43.0 (SD 10.3) years, mean height 1.74 (SD 0.04) m, mean body mass 73.5 (SD 1.3) kg] using a sledge apparatus with which both force and speed could be directly measured. Different after-loads were obtained by positioning the sledge at five different angles (SA, alpha) in respect to the horizontal so that m x g x sin alpha (where m is the sum of body mass and the mass of the sledge seat, g the acceleration due to gravity) decreased (on average) from 78% body mass at 30 degrees to 27% body mass at 10 degrees, thus simulating conditions of low gravity. The subjects were asked to jump maximally, without counter movement, starting from 70 degrees, 90 degrees, 110 degrees, and 140 degrees of knee angle (KA); the protocol being repeated at 10 degrees, 15 degrees, 20 degrees, 25 degrees and 30 degrees SA. The average (W+(mean)) power output during concentric exercise (CE) was found to decrease when the starting KA was increased, but to be unaffected by SA (i.e. by the after-load, the simulated low g). The higher values of W+(mean) were recorded at 90 degrees KA [15.01 (SD 1.46) W x kg(-1), average for all subjects at all SA]. The subjects were also asked to perform counter movement (CMJ) and rebound jumps (RE) at the same SA as for CE. In CMJ and RE maximal power outputs were also found to be unaffected by the SA; W+(mean) amounted to 16.03 (SD 0.28) W x kg(-1) in CMJ and 16.88 (SD 0.36) W x kg(-1) in RE (average for all subjects at all SA). In CE, CMJ and RE, the instantaneous force at the onset of the positive speed phase (F(i)) was found to increase linearly with SA (i.e. with increasing m x g x sin alpha), and the difference between F(i) in CMJ or RE and F(i) in CE (F(i) in CMJ minus F(i) in CE and F(i) in RE minus F(i) in CE) was unaffected by SA. This indicated that both maximal power and the elastic recoil were unaffected by simulated low g ranging from 1.71 m x s(-2) (at 10 degrees SA) to 4.91 m x s(-2) (at 30 degrees SA).
The maximal explosive power during a two legs jump was measured on four competitive athletes [mean age 24(SD 4.3) years; height 1.79 (SD 0.09) m; body mass 68.7 (SD 12.8) kg] at different starting knee angles (70, 90, 110, 130 and 150 degrees). The experiments were performed on a newly developed instrument with which both force and speed could be measured using a force platform and a wire tachometer, respectively, and on a conventional force platform. At the smallest knee angle (70 degrees) the mean power output (W in watts per kilogram) developed during the jump was found not to differ significantly between the two methods (P > 0.1). At the larger knee angles W was 18.4% (90 degrees), 34.5% (110 degrees), 47.4% (130 degrees) and 19.4% (150 degrees) higher using the conventional force platform (P < 0.05 throughout). The difference of W between the two methods was attributed to the recovery of elastic energy due to the counter movement which immediately preceded the jump on the conventional platform, but not on the newly developed instrument. Indeed because of a mechanical arrangement which prevented the subject from moving towards the platforms, eccentric work (W-) could not be performed on the newly developed instrument; whereas W- on the conventional force platform was almost negligible at 70 degrees knee angle [mean 1.7 (SD 2.3 J)] reached a maximum of 13.1 (SD 7.9) J at 130 degrees and decreased again to a mean 4.7 (SD 3.6) J for the largest angle (150 degrees). Furthermore, on the conventional force platform, the force at the onset of the positive speed phase (Fi) was an increasing function of W- (r2 = 0.519, P < 0.001); and the difference of W between the conventional and new instruments was larger the larger the difference of Fi (r2 = 0.391, P < 0.01).
To determine the effects of 6-weeks of heavy-resistance training on physical fitness and serum hormone status in adolescents (range 14-16 years old) 19 male handball players were divided into two different groups: a handball training group (NST, n = 10), and a handball and heavy-resistance strength training group (ST, n = 9). A third group of 4 handball goalkeepers of similar age served as a control group (C, n = 4). After the 6-week training period, the ST group showed an improvement in maximal dynamic strength of the leg extensors (12.2%; P < 0.01) and the upper extremity muscles (23%; P < 0.01), while no changes were observed in the NST and C groups. Similar differences were observed in the maximal isometric unilateral leg extension forces. The height of the vertical jump increased in the NST group from 29.5 (SD 4) cm to 31.4 (SD 5) cm (P < 0.05) while no changes were observed in the ST and C groups. A significant increase was observed in the ST group in the velocity of the throwing test [from 71.7 (SD 7) km x h(-1) to 74.0 (SD 7) km x h(-1); P < 0.001] during the 6-week period while no changes were observed in the NST and C groups. During a submaximal endurance test running at 11 km x h(-1), a significant decrease in blood lactate concentration occurred in the NST group [from 3.3 (SD 0.9) mmol x l(-1) to 2.4 (SD 0.8) mmol x l(-1); P < 0.01] during the experiment, while no change was observed in the ST or C groups. Finally, a significant increase (P < 0.01) was noted in the testosterone:cortisol ratio in the C group, while the increase in the NST group approached statistical significance (P < 0.08) and no changes in this ratio occurred in the ST group. The present findings suggested that the addition of 6-weeks of heavy resistance training to the handball training resulted in gains in maximal strength and throwing velocity but it compromised gains in leg explosive force production and endurance running. The tendency for a compromised testosterone:cortisol ratio observed in the ST group could have been associated with a state of overreaching or overtraining.
Little is known about the molecular mechanism underlying the release of merozoites from malaria-infected erythrocytes. In the present study, video microscopy was carried out, and images throughout the process of merozoite release from Plasmodium falciparum-infected erythrocytes were digitized and analyzed. Merozoites were shown to escape from the infected host cell in about 1 s through a single site of the infected erythrocyte membrane, whose dimension was estimated to be 2.5 microm. Merozoites were released together with the residual body containing hemozoin, leaving behind a membranous structure that persisted even after an extended period of observation. Densitometric measurements showed that the cytoplasmic content of the infected erythrocyte did not diffuse out as parasites were released, but was gradually lost thereafter. This would indicate that the release of merozoites from infected erythrocytes is not mediated by an explosive event.
Intermittent explosive disorder (IED) may best be thought of as a categoric expression of recurrent, problematic impulsive aggressive behavior. Although diagnostic criteria issues have made systematic research in IED difficult, recent work with new research criteria may allow for important empiric work to take place. Given that previous research in the area of impulsive aggression has been highly informative concerning the genetics, biology, and pharmacologic treatment of this behavior, application and extension of this work to IED is crucial. This paper reviews several important aspects of IED including its history in the Diagnostic and Statistical Manual of Mental Disorders, the formation of new research criteria, and the phenomenologic, epidemiologic, genetic, biologic, and treatment correlates of this disorder.
Morphine sulfate is a weak analgesic in the frog Rana pipiens pipiens, causing a slight increase in nociceptive threshold at a dose of 10 mg/kg and a pronounced increase at 100 mg/kg. Morphine-induced analgesia persists for at least 165 min and is significantly attenuated by naloxone. The analgesic doses of morphine are well below the lethal dose and are without noticeable effect on the behavior of the frogs or their responses to non-painful stimuli. Higher doses of morphine (320 and 640 mg/kg) induced a state of hyper-responsiveness to sensory stimuli similar to the explosive motor behavior induced in rats by microinjection of morphine into the periaqueductal gray.
The nature of the functional interaction between neostriatal dopamine activity and collicular GABA activity was studied. To this end we analyzed the ability of apomorphine injections into the neostriatum (50-500 ng/0.5 microliters per side) to reinitiate explosive running behaviour in rats pretreated with a subthreshold dose of picrotoxin into the colliculus superior (30-80 ng/0.5 microliters per side). Apomorphine was found to reinitiate the former behaviour dose dependently and its effect could be antagonized by a very low dose of neostriatally applied haloperidol (250 ng/0.5 microliters per side). It was possible to delineate the most critical region within the structures studied. Evidence is presented in favour of the previously reported hypothesis that increasing dopaminergic neostriatal activity within the terminal region of the nitrostriatal pathway results in a decreasing GABAergic activity within the terminal region of the GABAergic striato-nigro-collicular pathway.
Glass shrapnel from explosions of "dry ice bombs," created by thrusting dry ice into glass soft-drink bottles and occluding the opening of the bottles, has produced serious and multiple lacerating injuries in three children, which required major operative intervention. We found no previous reports of this grave hazard.