[Can the reactions of lysine oxidative deamination be used for the biochemical differentiation between Salmonella sp. and Citrobacter freundii?].
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A 61 year old male with a localized gastric cancer developed a post-operative pneumonia by C. diversus which was isolated from blood and from bronchoalveolar lavage. A satisfactory response was observed after treatment with penicillin and amikacin. A brief review of the literature concerning the clinical relevance of infections by this unusual agent is offered.
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A report is given on the biochemical properties of C. amalonaticus-strains of avian origin. Some strains show decisive deviations of the "norm", usually established from mammalian strains and details are given in a table. The results are discussed and the strains considered to be avian specific.
Specific activity of glutamate dehydrogenase (GD) and glutamate synthase (GtS) has been determined in the wild strain C3 and on a non excreting pro- mutant strain. Methionine sulfone shows inhibitory effects on their growth. The addition of alpha-ketoglutarate to the medium prevents the inhibitory effect and increases the GtS specific activity in both strains. The physiological effect of methionine sulfone and its suppression by alpha-ketoglutarate is discussed.
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pANV-6 is a 5.85 kb, streptomycin resistant, high copy number plasmid isolated from a multi drug resistant clinical isolate of C. diversus by transformation in Escherichia coli C-600. The plasmid was very stable, noncurable and could not be amplified with chloramphenicol. It was non-conjugative among Enterobacteriaceae hosts. Plasmid was transferred by transformation to several Gram negative and Gram positive hosts. These included Esch. coli, Serratia marcescens, Salmonella typhimurium, Klebsiella pneumoniae, Staphylococcus aureus, Corynebacterium glutamicum. From Staph. aureus it was transferred by conjugation to other Staph. aureus, Staph. epidermidis and Bacillus subtilis. Plasmid was incompatible with standard plasmids belonging to incompatibility groups H1, H2 = S,J,P and T of coliform bacteria. Plasmid has one site for restriction enzyme EcoRI and Pst I, three for Bgl 1 and non for Bgl 1, Bam HI, Hind III and X ba I.
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