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Results for “Trabecular Meshwork”

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At least 613 records · Page 34Linked to original sources
PubMed1994

[Effects of porcine pancreatic elastase-1 on elastin in human trabecular meshwork--immunohistochemical studies: Report 3. Primary open angle glaucoma].

The aim of this study was to determine the location of elastin in the extracellular material and to estimate the influence of porcine pancreatic elastase-1 (PPE) on the elastin in human trabecular tissue. Trabecular tissues obtained from normal post-mortem eyes and trabeculectomy specimens of primary open angle glaucoma (POAG) were used in this study. The tissues were embedded in Lowicryl K4M and sectioned for electron microscopy. First, the sections were subjected to protein A-gold immunohistochemical staining to determine the location of elastin in the tissues. Second, the sections were exposed to PPE, before immunostaining, to evaluate the change of immunolocalization of the gold particles in the tissue. The results were as follows. In POAG specimens, gold particles indicating the presence of elastin were located in the area of fine fibrillar-like material in the subendothelial layers of Schlemm's canal in POAG specimens. In normal eyes, few gold particles were localized in the area. The density of gold particles in the area was reduced by PPE in POAG specimens. These results show that elastin is localized in the area of fine fibrillar-like material in the subendothelial layers of Schlemm's canal of POAG, and that PPE dissolves the elastin in the area.

Aged↗
PubMed1993

[Effects of porcine pancreatic elastase-1 on the elastin in human trabecular meshwork--immunohistochemical studies: report 1].

The aim of this study was to determine the localization of elastin and to estimate the influence of porcine pancreatic elastase-1 (PPE) and alpha 1-antitrypsin-elastase complex (alpha 1A-EL), a circulating form of PPE, on the elastin in human trabecular tissue. Trabecular tissues obtained from normal postmortem eyes and trabeculectomy specimens of primary open angle glaucoma (POAG) were used in this study. The tissues were embedded in Lowicryl K4M and sectioned for electron microscopy. Then the sections were subjected to protein A-gold immunohistochemical staining to determine the localization of elastin in the tissues. The sections were exposed to varying concentrations of PPE or alpha 1A-EL, before immunolabeling, to evaluate the density of the gold particles in the tissue. The gold particles were localized in the elastic fibers of normal and POAG specimens. The density of gold particles was reduced by relatively low concentrations of PPE and alpha 1A-EL in both normal and POAG specimens. These results suggest that elastin is localized in the elastic fibers of human trabecular tissues, and relatively low concentrations of PPE or alpha 1A-EL dissolve the elastin in the tissues.

Adult↗
PubMed1986

Factors influencing glycosaminoglycan synthesis by calf trabecular meshwork cell cultures.

When cell or explant cultures were established from excised calf aqueous outflow pathway tissue and subsequently labeled with radioactive precursors of glycosaminoglycans [( 3H]glucosamine and [35S]sulfate), hyaluronic acid was the predominant glycosaminoglycan produced initially. As the cultures reached confluence, sulfated glycosaminoglycans became more prominent products. Further investigation revealed that the cultured trabecular cells could synthesize all of the glycosaminoglycans found in isolated calf trabecular tissue once a critical cell density had been attained. Increasing concentrations of fetal bovine serum in the culture medium stimulated glycosaminoglycan accumulation without altering the quantitative distribution of the various glycosaminoglycan products. Treatment of confluent monolayers with enzymes capable of degrading extracellular matrix molecules caused marked changes in the distribution of glycosaminoglycans produced by calf trabecular-cell cultures. The results suggest that calf trabecular cell cultures can be used to study the dynamics of extracellular matrix production and turnover under controlled experimental conditions.

Animals↗