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Results for “Reagent Strips”

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At least 613 records · Page 34Linked to original sources
PubMed1996

Post-amplification primer extension of heat-denatured AmpliType PCR products: effects on typing results.

Alleles of the HLA, DQA1, LDLR, GYPA, HGBB, D7S8 and GC loci, which are amplified using the AmpliType(R) PM PCR Reaction Mix and Primer Set, can be detected using sequence-specific oligonucleotide probes immobilized on a nylon membrane strip. Using reagents supplied in AmpliType PCR Amplification and Typing Kits, patterns of blue dots corresponding to particular alleles are visualized on the DNA probe strips. Frequently, the correct interpretation of typing results is dependent not only on the presence of probe signals but also on their relative intensities. The relative probe signal intensities obtained from an undegraded DNA sample extracted from a single individual will be different from those obtained from degraded DNA and from samples containing DNA from more than one source. Because probe signal intensity is an essential consideration for interpretation, factors that can influence it need to be identified. Clearly, the time and temperature of the assay steps and the salt concentration in the typing solutions can affect probe signal intensity. Also, if heat-denatured PCR products are allowed to cool for several minutes, the strands will reanneal and become unavailable for binding to the probes immobilized on the strips. However, the selective loss of GC B and HLA DQA1 4.1 probe signals observed after shorter cooling times cannot be explained by these factors. We demonstrate that following heat denaturation of PM PCR products there is sufficient residual Taq DNA polymerase activity to extend primers as the solution cools and that this primer extension occurs at a more rapid rate than PCR product reannealing. Primer extension across probe binding sites will prevent hybridization of the PCR product to complementary probes on the strip. The extent of signal reduction is dependent on the position of the probe binding site relative to the 3' ends of the primers and on the strand to which the probe is complementary. We recommend a simple modification to the AmpliType typing protocol to ensure all probe binding sites will be available for hybridization to PM and HLA DQA1 DNA probe strips.

DNA↗
PubMed2000

The urine test strip of the future.

The aim of the classical multiple test strip is to perform routine chemical analysis in one single operation, yielding maximum diagnostic and prognostic information. A maximum of 10 test areas should not be exceeded. The efficiency of the currently marketed test strips is discussed and improvements within acceptable price limits are suggested.

Biomarkers↗
PubMed1989

Simplified method to estimate salivary buffer capacity.

A new and simplified method to estimate the salivary buffer capacity was tested. The method, Dentobuff Strip, consists of a pH indicator paper that has been impregnated with acid. A small volume of saliva is added to the strip and after 5 min the color of the strip is compared with a chart. The colors have been chosen to indicate low, medium, or good buffer capacity. The buffer capacity of stimulated saliva samples from 62 subjects was measured with the new method and compared with two commonly used clinical methods, Dentobuff, and an electrometric method. The three methods correlated well, and patients with a low salivary buffer capacity (final pH less than or equal to 4), according to the electrometric method, were readily identified with the new Dentobuff Strip method.

Buffers↗