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[Determination and survey of hexachlorobenzene in food red nos. 104 (phloxine) and 105 (rose bengale) by GC/MS].

The contents of hexachlorobenzene (HCB) in Food Red Nos. 104 and 105 samples certified in fiscal years 1998-2001 were determined by GC/MS. All 32 samples contained HCB ranging from 0.2 to 32.9 mg/kg. The daily intakes of HCB based on the production of food colors was estimated to be from 0.018 to 1.456 ng, and their average (0.441 ng) corresponded to 0.0052% of the ratio for the Tolerable Daily Intake (8.5 micrograms for 50 kg body weight) of HCB developed by the IPCS.

Fluoresceins↗

Measurement of hepatic blood flow in the unanesthetized rabbit using 198Au and 125I Rose Bengal clearance technique.

Hepatic blood flow was measured in the unanesthetized rabbit using the clearance technique of 198Au and 125I RB. The values are: 71.82 +/- 16.24 ml-min-1-kg-1 for 198Au, and 60.21+/-9.94 ml-min-1-kg-1 for 125I RB (P less than 0.01). The overestimation of HBF with colloidal gold is probably due to extra splanchnic sequestration which has been estimated to be 15+/-3%. The week extraction of RB limits the use of this dye for measurements of HBF. HBF is easier to measure with 198 Au.

Animals↗

Multiphoton-excited microfabrication in live cells via Rose Bengal cross-linking of cytoplasmic proteins.

We demonstrate the use of multiphoton-excited photochemistry to cross-link three-dimensional matrices directly from cytoplasmic proteins in a live cell (starfish oocyte). Fluorescence recovery after photobleaching measurements were used to determine diffusion coefficients inside intracellular cross-linked structures, and it was found that the diffusion was approximately 3 to 4 orders of magnitude slower than in free solution and 2-3 orders of magnitude slower than in cytoplasm and that the value can be tuned by controlling the laser exposure. Complex structures can be fabricated to construct channels and compartments that could be used to isolate cellular processes, and the method should thus be applicable to a broad range of problems in cell biology.

Animals↗

A clinical comparison of 99mTc-diethyl-iminodiacetic acid, 99mTc-pyridoxylideneglutamate, and 131I-rose bengal in liver transplant patients.

The relative merits of three radiopharmaceuticals for evaluating liver transplant patients were determined in paired studies. In 86% of studies both 131I-RB and 99mTc-PG gave similar information for differentiation of hepatocellular disease and billary tract obstruction. 99mTc-PG probably demonstrates the biliary tract and small intestine better early after injection (8%); 131I-RB is probably better in showing the colon at 24 hours when intestinal activity is not seen by 1 hour (6%). 99mTc-diethyl-IDA is superior in all respects when compared to 99mTc-PG. The blood retention method (20 min./5 min.) showed that none of the radiopharmaceuticals was a reliable indicator of hepatocyte function when compared to total serum bilirubin.

Biliary Tract Diseases↗

[Hepatic function in circulatory insufficiency. Mathematical modeling of 137I-labelled rose bengal transport in the body].

131I-labelled bengali pink was used to perform radiohepatography in patients with circulatory failure. Both amplitude-time parameters of blood clearance curves and hepatograms and the generalized index of parameters of the linear 3-chambered reversible model of this tracer transport in the body indicate that the decrease of hepatic absorptive and excretory function in the presence of 131I-labelled bengali pink occurs in a direct relationship to the degree of circulatory insufficiency and developed in parallel with bilirubin metabolic disturbances. In some patients, hepatic lesion in the presence of Stages IIB-III circulatory insufficiency with signs of hepatocellular failure and cholestasis was accompanied only by moderate hepatic absorptive and excretory dysfunction against 131I-labelled bengali pink and by an increase in blood total bilirubin levels no more than that in Stage IIA circulatory insufficiency.

Bile↗