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At least 613 records · Page 34Linked to original sources
PubMed2006

Human papilloma virus in hyperplastic tonsillar and adenoid tissues in children.

BACKGROUND: Human papillomavirus (HPV) in childhood causes skin and anogenital warts as well as the recurrent respiratory papillomatosis, a life-threatening cause of upper airway obstruction in children. To date, the information on HPV infection in tonsillar and adenoid hyperplasia in children is limited. PURPOSE: The purpose of this study was to investigate the presence of HPV DNA in children with benign tonsillar and/or adenoid hyperplasia. METHODS: One hundred six samples of paraffin-embedded adenoid and/or tonsillar tissues from 102 children, 57 girls and 45 boys (age range, 2-14 years), were tested for the presence of HPV DNA using polymerase chain reaction (PCR) with general primers GP5+/GP6+. HPV typing was performed by PCR with specific primers for HPV-16, -18, -33 and -11. RESULTS: HPV DNA was detected in 9 (8.5%) of the 106 collected specimens. The frequencies of HPV typing were 6 of 9 (66.7%) for HPV-16, 2 of 9 (22.2%) for HPV-11, zero of 9 (0%) for HPV-33 and HPV-18, whereas one HPV-positive sample remained untyped. No multiple HPV infection was detected. HPV was detected in 6 (9.4%) children with tonsillar hyperplasia and in 3 (7.1%) with adenoid hyperplasia. The mean age of children with HPV-positive specimens was lower than that of HPV-negative children (P = 0.006). No statistical correlation in the prevalence of HPV infection was observed according to children's sex, origin or residence (urban or rural). CONCLUSION: Although the significance of the presence of HPV DNA in tonsillar and adenoid hyperplasias remains obscure, the PCR detection of high-risk HPV DNA should be evaluated cautiously.

Adenoids↗
PubMed1964

MAMMOGRAPHY.

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Animals↗
PubMed1964

LOBULOALVEOLAR DIFFERENTIATION IN MOUSE MAMMARY TISSUES IN VITRO.

Administration of lobuloalveolar mammogenic hormones (for a period insufficient to induce alveolar differentiation) to immature mice prior to cultivation of their mammary glands in chemically defined, hormone-supplemented media resulted in lobuloalveolar growth in vitro comparable to that of late pregnancy. No such growth occurred in cultures of mammary tissues from mice not treated with hormones. The use of tissues from hormone-treated donors might serve as a valuable tool for studies concerned with differentiation in vitro.

Aldosterone↗
PubMed1964

DNA SYNTHESIS IN ALVEOLAR CELLS OF THE MAMMARY GLAND: ACCELERATION BY OVARIAN HORMONES.

In normal alveolar cells of the mammary gland of C3H/HeJ female mice, DNA synthesis lasts an average of 20.7 hours with a coefficient of variation of 26.6 percent. Treatment of the mice with I microgram of 17-beta-estradiol and 1 milligram of progesterone per day for 3 to 4 days decreases the average duration to 10.7 hours and the coefficient of variation to 13.8 percent.

Alveolar Epithelial Cells↗
PubMed1964

MATERNAL FACILITATION OF SUCKING DRIVE IN NEWBORN LAMBS.

Although active maternal assistance is not essential for the newborn lamb to find the teats and suck for the first time, the process is facilitated by grooming and by directional orientation of the ewe toward the lamb. Neither orientation nor grooming alone facilitated the sucking drive to any significant extent.

Animals↗
PubMed1965

GROWTH OF MOUSE MAMMARY GLANDS IN VIVO AFTER MONOLAYER CULTURE.

Mouse mammary tissue grown in primary cell culture did not display specialized characteristics of the parent tissue. When implanted into gland-free mammary fat pads, not only did some of these cultured cells grow and produce normal mammary outgrowths, but some of the outgrowths formed glands which displayed a variety of culture-induced abnormalities.

Animals↗
PubMed1965

SPECTROPHOTOMETRIC CHARACTERIZATION OF PIGMENTS PRODUCED BY STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BOVINE UDDERS.

Sandvik, Olav (National Animal Disease Laboratory, Ames, Iowa), and Richard W. Brown. Spectrophotometric characterization of pigments produced by Staphylococcus epidermidis strains isolated from bovine udders. J. Bacteriol. 89:1201-1208. 1965.-Spectrophotometric analysis of methanol-extracted pigments of 70 isolates, representing 48 strains of Staphylococcus epidermidis, exhibited absorption curves that were classified into seven types, designated I, II, III, IV, V, VI, and a S. aureus type. Two subtypes were included in types I and III. All nonpigmented cell extracts were classified as type I. One of the six numbered type absorption curves was obtained with the majority of isolates. Three isolates gave an absorption curve that was similar to the curve produced by extracts of five of seven S. aureus strains, thus the designation S. aureus type. The differences in pigment complexes indicated by the various absorption curves of methanol extracts were substantiated by column-chromatography studies. Generally, pigments of types II to VI, as produced by representative strains, were of a xanthophyllic nature, whereas, the S. aureus type pigment studied was carotenelike. Analyses of representative strains showed that the type of spectral absorption curves did not change whether the organisms were carried in vitro and tested through 3 months, or isolated repeatedly from infected udders for periods up to 8 months. The method of determining the spectral absorption curves of whole-cell methanol extracts provides an additional tool for differentiating strains of S. epidermidis that can be used in enzootiological studies of udder infections.

Animals↗