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At least 613 records · Page 34Linked to original sources
PubMed2004

Slow-light six-wave mixing at low light intensities.

We report an experimental study of resonant six-wave mixing in coherently prepared Rb atoms. Electromagnetically induced transparency in a four-level atomic system suppresses the linear susceptibility and enhances the nonlinear susceptibilities, which leads to the resonantly enhanced, slow-photon six-wave mixing at low light intensities. The light emission in the six-wave mixing process can be viewed as resulting from diffraction of slow light off a resonant nonlinear grating induced in the four-level system by a standing-wave pump field.

Journal Article↗
PubMed2005

Current-induced light emission and light-induced current in molecular-tunneling junctions.

The interaction of metal-molecule-metal junctions with light is considered within a simple generic model. We show, for the first time, that light-induced current in unbiased junctions can take place when the bridging molecule is characterized by a strong charge-transfer transition. The same model shows current-induced light emission under potential bias that exceeds the molecular excitation energy. Results based on realistic estimates of molecule-lead coupling and molecule-radiation field interaction suggest that both effects should be observable.

Journal Article↗
PubMed2006

Low-light-level cross-phase-modulation based on stored light pulses.

We experimentally demonstrate a low-light-level cross-phase-modulation (XPM) scheme based on the light-storage technique in laser-cooled 87Rb atoms. The proposed scheme can achieve a similar phase shift and has the same figure of merit as one using static electromagnetically induced transparency under the constant coupling field. Nevertheless, the phase shift and the energy loss of a probe pulse induced by a signal pulse are neither influenced by the coupling intensity nor by the atomic optical density in the light-storage XPM scheme. This scheme enhances the flexibility of the experiment and makes possible conditional phase shifts on the order of pi with single photons.

Journal Article↗
PubMed1998

Light-regulated transcription of genes encoding peridinin chlorophyll a proteins and the major intrinsic light-harvesting complex proteins in the dinoflagellate amphidinium carterae hulburt (Dinophycae). Changes In cytosine methylation accompany photoadaptation

In the dinoflagellate Amphidinium carterae, photoadaptation involves changes in the transcription of genes encoding both of the major classes of light-harvesting proteins, the peridinin chlorophyll a proteins (PCPs) and the major a/c-containing intrinsic light-harvesting proteins (LHCs). PCP and LHC transcript levels were increased up to 86- and 6-fold higher, respectively, under low-light conditions relative to cells grown at high illumination. These increases in transcript abundance were accompanied by decreases in the extent of methylation of CpG and CpNpG motifs within or near PCP- and LHC-coding regions. Cytosine methylation levels in A. carterae are therefore nonstatic and may vary with environmental conditions in a manner suggestive of involvement in the regulation of gene expression. However, chemically induced undermethylation was insufficient in activating transcription, because treatment with two methylation inhibitors had no effect on PCP mRNA or protein levels. Regulation of gene activity through changes in DNA methylation has traditionally been assumed to be restricted to higher eukaryotes (deuterostomes and green plants); however, the atypically large genomes of dinoflagellates may have generated the requirement for systems of this type in a relatively "primitive" organism. Dinoflagellates may therefore provide a unique perspective on the evolution of eukaryotic DNA-methylation systems.

Journal Article↗
PubMed1986

A Semidian Rhythm in the Flowering Response of Pharbitis nil to Far-Red Light: I. Phasing in Relation to the Light-Off Signal.

Evidence is presented of an endogenous rhythm in flowering response to far-red (FR) irradiation, with a period of about 12 h (hence semidian rhythm), which persists through at least three cycles in constant conditions of continuous light at 27 degrees C and has a marked influence on the flowering response in Pharbitis nil to a subsequent inductive dark period. The phase of the rhythm is not influenced by real time nor by the time from imbibition or from the beginning of the light period. Rather, it is fed forward from the beginning of the FR interruption to the beginning of the inductive dark period. The period of the rhythm is not affected by irradiance but is longer at cooler temperature. When there are two FR interruptions during the preceding light period, it is primarily the later one which determines the phase of the rhythm, although some interactions are evident. There appears to be an abrupt rephasing of the rhythm at the beginning of the inductive dark period. No overt rhythms which could be used as "clock hands" for the semidian rhythm were detected in photosynthesis, stomatal opening, or translocation.

Journal Article↗
PubMed1987

Dynamics of Photosystem II and Its Light Harvesting System in Response to Light Changes in the Halotolerant Alga Dunaliella salina.

A photosystem two (PSII) core complex consisting of five major polypeptides (47, 40, 32, 30, and 10 kilodaltons) and a light harvesting chlorophyll a/b complex (LHC-2) have been isolated from the halotolerant alga Dunaliella salina. The chlorophyll and polypeptide composition of both complexes were compared in illuminated and dark-adapted cultures. Dark adaptation is accompanied by a decrease in the chlorophyll a to chlorophyll b (Chl a/Chl b) ratio of intact thylakoids without any change in total chlorophyll. These changes occur with a half-time of 3 hours and are reversed upon reillumination. Analyses of PSII enriched membrane fragments suggest that the decrease in the Chl a/Chl b is due partly to an increase in the Chl b content of LHC-2 and partly to changes in the relative levels of the two complexes. Apparently during dark adaptation there is: (a) a net synthesis of chlorophyll b, (b) removal of PSII core complexes resulting in a 2-fold drop in the PSII cores to LHC-2 chlorophyll ratio. These changes should dramatically increase the light harvesting capacity of the remaining PSII reaction centers. Presumably this adjustment of antenna size and composition is a physiological mechanism necessary for responding to shade conditions. Also detected, using (32)P, are light-induced phosphorylation of the LHC-2 (consistent with the ability to undergo State transitions) and of the 40 and 30 kilodalton subunits of the PSII core complex. These observations indicate that additional mechanisms may also exist to help optimize the interception of quanta during rapid changes in illumination conditions.

Journal Article↗
PubMed2004

Benign summer light eruption and polymorphic light eruption: genetic and functional studies suggest that a revised nomenclature is required.

New research indicates that polymorphic light eruption (PLE) is an autoimmune disease against an ultraviolet radiation-induced cutaneous antigen. PLE may even confer some protection against skin cancer later in life. This new information demands a reassessment of the precise nature and nomenclature of PLE. Benign summer light eruption (BSLE) (lucite estivale bénigne) is the name used in continental Europe, and particularly France, to describe a clinically short-lived, itchy, papular eruption particularly affecting young women after several hours of sunbathing at the beginning of summer or on sunny vacations. Clinically more prolonged forms of solar eruption, starting early in spring and persisting for long periods, have been known in France as polymorphic light eruption (PLE) (lucite polymorphe) ('European PLE'). Investigative studies, however, now suggest that BSLE and some cases of 'European PLE' are part of the same spectrum. In the Anglo-Saxon literature, they are lumped together as PLE ('Anglo-Saxon PLE'). The other cases of 'European PLE', which do not fall within the compass of 'Anglo-Saxon PLE', are, in the Anglo-Saxon literature, classified as either actinic prurigo (AP) (a genetically determined, prolonged, excoriated form of Anglo-Saxon PLE), or chronic actinic dermatitis (CAD) (a sunlight-induced eczema precisely resembling allergic contact dermatitis, apparently to an ultraviolet radiation-induced antigen). It is therefore proposed that: i. the European term BSLE be dropped and that these patients be reclassified within the spectrum of (Anglo-Saxon) PLE, ii. the European use of the term PLE ('European PLE') be discontinued, iii. those previously diagnosed as having 'European PLE' be reclassified as (Anglo-Saxon) PLE, AP or CAD, as appropriate. The benefits of such a change in nomenclature would be twofold, firstly a uniformity of terminology and secondly, and more importantly, terminology would then correlate better with our recently improved understanding of the pathogenesis of these disorders.

Journal Article↗
PubMed2003

Liquid light guides versus fiber light guides in clinical near-infrared spectroscopy.

New commercial liquid light guides have an advantage over fiberoptic bundles regarding breakage during clinical handling. We investigate the quality of clinical data collection using liquid versus fiber bundles as receivers. A four-wavelength NIRO-500 near-IR spectrophotometer is used with single-terminal fiber bundles, multiterminal fiber bundles, or a single-terminal liquid light guide as receivers. Repeated 3-min trials are done using a stable phantom, an unstable phantom, and the human forearm. A least-squares linear best-fit line and its root mean square error (RMSE), a measure of signal noise, are derived for each wavelength of each trial. The mean and standard deviations for the RMSEs of the single-terminal fiber optic receiving cable are derived for comparison standards. The liquid light guides have 51 to 174% greater signal noise with RMSEs 2 to 12 standard deviations above the mean of the single-terminal fiber bundle. The multiterminal fiber bundles have 49% less to 32% greater signal noise and had RMSEs within 1 to 4 standard deviations above the mean of the single-terminal fiber bundle. These comparisons suggest fiber optic bundles are preferable for clinical near-IR spectroscopy (NIRS) applications requiring low signal noise.

Blood Volume↗
PubMed2003

Phase I drug and light dose-escalation trial of motexafin lutetium and far red light activation (phototherapy) in subjects with coronary artery disease undergoing percutaneous coronary intervention and stent deployment: procedural and long-term results.

BACKGROUND: Motexafin lutetium (MLu; Antrin) is a photosensitizer that is taken up by atherosclerotic plaque and concentrated within macrophages and vascular smooth muscle cells. After photoactivation with far red light, MLu facilitates production of cytotoxic oxygen radicals that mediate apoptosis. We assessed the safety and tolerability of phototherapy (PT) with MLu in patients undergoing percutaneous coronary intervention with stent deployment. METHODS AND RESULTS: An open-label, phase I, drug and light dose-escalation clinical trial of MLu PT enrolled 80 patients undergoing de novo coronary stent deployment. MLu was administered to 79 patients by intravenous infusion 18 to 24 hours before procedure, and photoactivation was performed after balloon predilatation and before stent deployment. Clinical evaluation, serial quantitative angiography, and intravascular ultrasound were performed periprocedurally and at 6 months follow-up. MLu PT was well tolerated without serious dose-limiting toxicities, and side effects (paresthesia and rash) were minor. No adverse angiographic outcomes were attributed to phototherapy. CONCLUSIONS: This study demonstrates that coronary MLu PT seems safe, and the maximum well-tolerated MLu dose and range of tolerated light doses were identified. These data can be used in phase II efficacy trials of MLu PT for the treatment of coronary atherosclerosis or vulnerable plaque.

Adult↗
PubMedDate not supplied

Light and electron microscopic studies regarding cell contractility and cell coupling in light sensitive smooth muscle cells from the isolated frog iris sphincter.

(1) In light microscopical studies of living isolated frog irises, it was found that the maximal areas of experimentally light induced contractions in the m. sphincter pupillae were located beneath small illuminated regions. There were no visible contractions of muscle cells outside the illuminated areas. It was shown that exposure to light could directly cause contractions of isolated single sphincter muscle cells. (2) Junctional structures of the iris sphincter cells were studied by means of thin sections and freeze fracture electron microscopy. Intermediate junctions, a few focal tight junctions and occasional small gap junctions were identified. Pit containing intramembranous particles which resemble gap junctions connexons were found in large numbers, dispersed over the plasmalemmas of sphincter muscle cells. From these physiological and morphological observations, it is concluded that sphincter muscle cells of the frog iris may be coupled via gap junctions, but that the cell coupling is not sufficiently extensive to form the basis for a functional syncytium.

Animals↗
PubMed2006

Abrasion resistance of composites polymerized by light-emitting diodes (LED) and halogen light-curing units.

This study compared the abrasion resistance of direct composite resins cured by light-emitting diodes (LED) and halogen light-curing units. Twenty specimens (12 mm in diameter; 1.0 mm thick) of each composite resin [TPH (Dentsply); Definite (Degussa); Charisma (Heraus Kulzer)] were prepared using a polytetrafluoroethylene matrix. Ten specimens per material were cured with the LED source and 10 with the halogen lamp for 40 s. The resin discs were polished, submitted to initial surface roughness reading (Ra initial - microm) in a roughness tester and stored in water at 37 degrees C for 15 days. The specimens were weighed (M1) and submitted to simulated toothbrushing using slurry of water and dentifrice with high abrasiveness. After 100 minutes in the toothbrushing simulator, the specimens were cleaned, submitted to a new surface roughness reading (Ra final - microm) and reweighed (M2). Mass loss was determined as the difference between M1 and M2. Data were recorded and analyzed statistically by one-way ANOVA and Tukey Test at 5% significance level. The composite resin with greater size of inorganic fillers (TPH) showed the lowest mass loss and surface roughness means, indicating a higher resistance to toothbrush abrasion (p<0.05). Definite cured with LED presented the least resistance to toothbrush abrasion, showing the highest means of surface roughness and mass loss (p<0.05). The LED source did not show the same effectiveness as the halogen lamp for polymerizing this specific composite resin. When the composite resins were cured a halogen LCU, no statistically significant difference was observed among the materials (p>0.05). It may be concluded that the type of light-curing unit and the resin composition seemed to interfere with the materials' resistance to abrasion.

Acrylic Resins↗
PubMed1976

[RNA concentration in neuron-neuroglia systems of the retina and visual zones of the cerebral cortex during light deprivation and light stimulation].

A two-wave-length cytophotometry of the gallocyanin-chromium alum-stained preparations showed that in adult rats kept for 30 days in complete darkness there was a decrease in the RNA content in the perineuronal neuroglia of the retinal ganglion cell layer only, with no changes in the corresponding neurons. No changes were found in the neurons and in the perineuronal glia of the layer II---III of the visual cerebral cortex. After the end of light deprivation a 2-hour stimulation with a constant or flickering light did not influence the RNA content in the neurons of both regions of the visual analyzer studied, whereas in control rats this stimulation induced a marked increase in the RNA content in these neurons. Qualitative changes in the metabolism of the cellular RNA in the nervous system of adult animals under the effect of light deprivation are emphasized. Differences in the biochemical peculiarities of various neuron-neuroglia systems, depending on their localization in the visual analyzer, are discussed.

Animals↗
PubMed1992

Regulation and light-harvesting complex II association of a Dunaliella protein homologous to early light-induced proteins in higher plants.

The unicellular green alga Dunaliella bardawil responds to high light, nutrient deprivation, and several other types of stress by massive accumulation of beta-carotene. We have previously cloned a nuclear gene, cbr, that is co-induced with accelerated carotenogenesis. The predicted product of cbr is closely related to early light-induced proteins (Elips) of higher plants, and also shows resemblance to chlorophyll a/b-binding proteins. In the present study, the determination of the cbr transcription start site supported the previously proposed site of translation initiation. Antibodies raised against a synthetic oligopeptide matching the predicted sequence of Cbr recognized two polypeptides of apparently 17 and 19 kDa that were induced in parallel to cbr transcript accumulation in highly illuminated or sulfate-starved D. bardawil cells. The antibodies also cross-reacted with an approximately 20-kDa polypeptide in sulfate-starved Dunaliella salina. In both D. bardawil and D. salina, Cbr was found exclusively in the thylakoid membranes. After mild solubilization, Cbr co-fractionated with light-harvesting complex II (LHCII) in sucrose gradient centrifugation and gel electrophoresis and was specifically associated with a minor LHCII complex. An occasionally observed, faster mobility Cbr form, free of LHCII, was probably released from the larger complex. These results support the conclusion that Cbr belongs to a class of stress-induced proteins transiently associated with antennae complexes.

Amino Acid Sequence↗
PubMed1990

Morning light exposure for the treatment of winter depression: the one true light therapy?

The time of day when bright light should be administered to treat winter depression has become controversial. Results from clinical trials suggest that light given at any of several times of day can produce an antidepressant response. However, the basis for this effectiveness has been questioned and theoretical issues remain unresolved. It appears that individuals differ in their sensitivity to time of day as well as to dose. Unfortunately, important experimental details such as pretreatment severity of depression and order effects have not been taken into account, and this has made definitive conclusions impossible to reach. In order to understand the mechanism(s) by which light treatment works, we must discuss and employ common criteria for study design and data analysis. For clinical purposes, it currently seems that time of treatment can be adjusted to accommodate the physiological responsiveness and lifestyle requirements of individual patients.

Humans↗
PubMed1990

Analysis of clathrin light chain-heavy chain interactions using truncated mutants of rat liver light chain LCB3.

Clathrin light chains are extended molecules located along the proximal segment of each of the three heavy chain legs of a clathrin trimer. All mammalian light chains share a central segment with 10 repeated heptad motifs believed to mediate the interaction with clathrin heavy chains. In order to test this model in more detail, we have expressed intact rat liver clathrin light chain LCB3 in Escherichia coli and find that it binds tightly to calf clathrin heavy chains. Using a set of expressed truncated mutants of LCB3, we show that the presence of seven to eight heptads is indeed necessary for a successful interaction. More extensive deletions of the central segment completely abolish the ability to bind to heavy chains. Neither the amino- nor the carboxyl-terminal domain is essential for binding, but competition experiments show that the presence of the carboxyl-terminal domain does enhance the interaction with heavy chains.

Animals↗
PubMed1989

[Mechanism of cell damage by hematoporphyrin derivative (HPD) plus light. III. Effect of HPD plus light on lysosomes of liver and hepatoma cell].

The effects of HPD plus light on lysosome membrane and release of hydrolysis enzymes from lysosomes of normal rat liver and hepatoma cells were investigated. HPD was bound to lysosome either in vitro or in vivo. Lysosomes bound HPD plus light increased lipid peroxidation of unsaturated fatty acid of lysosome membrane and enhanced the activity of DNase, RNase, cathepsin and acid phosphatase. These effects were related to HPD concentration and exposure time but it was unchanged in the control. It was concluded that the enhancement of hydrolysis enzymes from lysosomes was due to the lysosome membrane damage under the action of siglet oxygen produced by HPD bound lysosomes following light activation.

Animals↗
PubMed1986

[Mechanism of cell damage by hematoporphyrin derivatives (HPD) plus light--I. Photooxidation of hepatoma cell membrane by HPD plus light].

Ascitic hepatoma cells of mice were incubated with three HPD. After the cell-bound HPD was treated by visible light, the product of membrane lipid peroxidation--malondialdehyde (MDA) was determined. The results showed that the MDA level increased with HPD concentration and exposure time, but it was unchanged in the control. Beta-carotene, a quencher of singlet oxygen, could inhibit the lipid photooxidation of the membrane of cell-bound HPD resulting in a decrease of MDA level which decreased with increase of beta-carotene concentration. Adding vitamin E in vitro and vivo, formation of the product of membrane lipid peroxidation of cell-bound HPD by light was inhibited and the MDA level decreased with the increase of vitamin E concentration. These results show that the singlet oxygen, produced by HPD bound cells following light activation, reacts directly with the polyunsaturated fatty acids on cell membrane to produce lipid peroxides leading to cellular damage.

Animals↗