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Results and policy implications of the resource-based relative-value study.

The resource-based relative-value scale (RBRVS) is a measure of relative levels of resource input expended when physicians produce services and procedures. It is a function of the physician's work input, the opportunity cost of specialty training, and the relative practice costs for each specialty. This paper presents resource-based relative values (RBRVs) for selected procedures of four major specialties--family practice, internal medicine, general surgery, and thoracic and cardiovascular surgery. We compare RBRVs with current charges and find several general patterns. Invasive procedures are typically compensated at more than double the rate of evaluation-and-management services, when both consume the same resource inputs. Imaging and laboratory procedures fall between invasive and evaluation-and-management services. We analyze the financial implications of the RBRVS by developing a simple model and simulating the effects of an RBRVS-based fee schedule on physicians' revenues in various specialties. We use Medicare data to perform the simulation under the "budget-neutral" assumption. Results show that an RBRVS-based fee schedule affects specialties differently. The average family practitioner could receive 60 percent more revenue from Medicare, whereas the average ophthalmologist could lose 40 percent of current revenues. The effects on other specialties fall between these two.

Economics, Medical↗

Impact of the BACTEC NR system in detecting Candida fungemia.

As a result of the growing importance of candidemia, various techniques for the detection of Candida spp. in blood have been designed with a view to speeding up the laboratory procedure. We have performed a retrospective comparison of the efficiencies of the conventional VACUTAINER system (Becton Dickinson Vacutainer Systems) and the BACTEC NR system (Johnston Laboratories, Inc.). During a 4-year period, 88,300 blood cultures were processed, with growth of Candida species in 552. The mean times required for the detection of growth were 7.55 days with the VACUTAINER system and 4.1 days with the BACTEC NR system (P less than 0.05). During the first week of incubation, the VACUTAINER system detected 56.1% of the candidemias and the BACTEC NR detected 93.6% (P less than 0.05). Use of the BACTEC NR therefore permits reduction of the incubation period from the previously established 4 weeks to a more convenient 7 to 14 days.

Candida↗

Physiological and toxicological aspects of smoke produced during the combustion of polymeric materials.

Normally one expects that flame contact is the major cause of injury and death during fires. Analysis of the factors involved in numerous fires has revealed that most deaths were not due to flame contact, but were a consequence of the production of carbon monoxide, nitrogen oxides, and other combustion products, such as aldehydes, low molecular weight alcohols, hydrogen cyanide, and other noxious species. The major emphasis within the scope of this paper relates to the physiological and toxicological aspects of smoke produced during the combustion of materials. Special emphasis is directed toward laboratory procedures which have been developed to determine the qualitative and quantitative analysis of smoke, factors pertaining to smoke development, and to measure the response of laboratory animals exposed to smoke. The effects that fire retardants, incorporated into polymeric materials as a means of improving flammability characteristics, may have on smoke development, the mechanism of polymer degradation, and on the survival response of laboratory animals are also considered.

Aldehydes↗

An alginate pick-up alternative to the altered cast technique.

The altered cast technique for distal extension removable partial dentures involves such cumbersome laboratory procedures that many dentists avoid using it. This article presents a simple and easy clinical technique that accomplishes the same objective, but involves no additional laboratory work. In addition, the technique provides superior accuracy when compared with the altered cast technique.

Alginates↗

Role of veterinarians in modern food hygiene.

Veterinary services and veterinary education and training must keep pace with the constantly changing patterns of agriculture and food processing. Changes in methods of animal production are associated with many problems of food processing and food quality. Veterinary supervision of the animal feed industry and of meat and distribution is essential. Quality testing of meat, milk, and eggs requires the introduction of suitable routine sampling systems, laboratory procedures, and complex evaluation procedures.Food hygiene problems have changed in recent years not only as a result of new methods of animal production, but also because of changes in food processing technology and in the presentation of food to the consumer, increased environmental pollution, increased international trade, and increased tourist travel.Food hygienists must adopt an active and progressive policy and change the scope of food control from a purely negative measure into a positive force working towards improved food quality and the avoidance of losses during production. A modern food hygiene programme should cover all stages of production, processing, and distribution of food and also other ingredients, additives and the water used for production and processing. Veterinarians should also be involved in the registration and licensing of enterprises and this should take into account the premises, the procedures to be used, new techniques in animal husbandry, machines and equipment, etc.In order to facilitate the microbiological analysis of foodstuffs, new mechanized or automated laboratory methods are required, and consideration must be given to adequate sampling techniques.

Animal Feed↗

In vitro cultivation of the rickettsial agent of trench fever.

Although trench fever appears to be endemic in many areas of the world, recognition of the disease has been handicapped by the difficulties of making a clinical diagnosis and the unavailability of a simple laboratory procedure to establish the etiology. The author describes a method for the in vitro cultivation of Rickettsia quintana that provides a relatively simple means for the laboratory diagnosis of trench fever. R. quintana can be propagated with ease from the blood of patients directly on blood agar incubated at 37 degrees C for 12-14 days under a gas tension of 5% CO(2) in air. The number of rickettsiae circulating in the patient's peripheral blood can be quantitated. The protracted rickettsiaemia in trench fever makes for a relatively long period during which blood culture can be usefully employed.In the course of studies with the method described, it was found that erythrocytes contain a factor (or, possibly, factors) essential for multiplication of R. quintana; this factor is cryostable and thermostable and may be haemoglobin. Blood serum also promotes multiplication.

Humans↗

[Our experience with a 24-hour on-call program in Nihon University Hospitals].

We must recognize that undergraduate clinical training is insufficient in Japan and that we need to improve the role of the laboratory medicine, especially in the area of communication. Therefore, postgraduate training in clinical pathology/laboratory medicine should include; 1. Basic clinical skills (Communication skills, physical examination and common laboratory procedures such as Gram's stain, Wright-Giemsa stain, etc.), 2. Principles, instrumentation and techniques of each discipline of this broad scientific field, 3. How laboratory information is used in a medical setting, 4. The role of the clinical pathologist/laboratory physician in interacting with laboratory staff, physicians and patients. There is an increasing gap between the clinic and the laboratory. Current needs require us to make a major attempt to bridge this gap. This effort will require new and resourceful means of communication and interaction. A 24-hour on-call program was established to enrich the training of the clinical pathology residents in the USA. Each resident in the program is responsible for being on call, which entails learning the day-to-day operation of the laboratory and being available to interpret laboratory data when the need arises. Residents are expected to refer to the appropriate staff member those situations that he or she feels unable to handle. A review of all on-call problems with staff provides to be a valuable education tool, both for the residents and the staff. This learning strategy was adopted from UCSF Hospital and introduced to Nihon University Itabashi Hospital by K. Kumasaka MD in 1984. And that after 12 years of actual experience, we conclude the training program provides a readily available consultative service for the house staff, and facilitates patient care. In addition, we should make rounds with physicians and go to conferences in areas of interest and responsibilities. We could be mainly responsible for a number of problem-solving areas within the laboratory that would be directly responsive to patient problems encountered by physicians.

Clinical Competence↗

Genetic testing and reporting: What the endocrinologists should know and can expect (Joint position paper of the ENDO-ERN).

Over the last decade, next generation sequencing (NGS) has become an essential tool for diagnostic DNA testing in human genetics. To improve the understanding of available genetic testing strategies and to facilitate the request of genetic testing in daily endocrine practice, clinical and laboratory experts in the field have summarized the major issues which should be known and considered. In this joint position paper of the ENDO-ERN, the roles and responsibilities of the health care professionals involved in the diagnostic workflow are described, and the major issues concerning genetic testing workflows are overviewed. These issues encompass all relevant steps, including test request and pre-analytical procedures, laboratory and data processing workflows, quality assurance, and reporting. As NGS procedures result in an increasing number of variants of unknown significance and incidental findings, these aspects are addressed as well. Accompanied by illustrations of the genetic diagnostic workflow and of concise reports for a fast orientation about the major aspects of genetic testing, this joint paper should support the health care professionals during a request for genetic testing.

VUS↗

Diagnostic modalities for infectious diseases.

The role of the microbiology laboratory is essential to successful provision of patient care. Obtaining appropriate laboratory data allows clinicians the best opportunity to arrive at a definitive diagnosis in a timely manner, eliminates the need for further tests, and can offer specific treatment approaches with maximal potential for success. This article discusses the laboratory procedures useful for diagnosis of oral or maxillofacial infections, including collection and transport of microbial specimens, representative techniques and methods available for culture and diagnostic assays, diagnostic laboratory principles for specific types of microbial etiologies, antimicrobial sensitivity, quality control issues, and evaluation of reported findings.

Bacterial Infections↗

Practical aspects of synovial fluid aspiration. The approaches to major extremity joints and examination of the aspirate.

Use of a few simple office laboratory procedures can provide accurate diagnosis of several forms of common diseases of joints. The average physician can discover unsuspected joint disease in this way, thereby avoiding expensive laboratory tests and delay in making the diagnosis. An adequate history and physical examination are needed in conjunction with analysis of fluid aspirated from the joint.

Ankle Joint↗

Minimizing biopsy error.

Several factors influence substantially the order of accuracy of oral biopsy procedures. This paper evaluates some of these factors and suggests methods whereby accuracy can be improved. The surgeon's responsibility extends beyond the operating theater and into the laboratory if maximum value is to be derived from each biopsy specimen. It is suggested that present sucess rates of surgical treatment of oral carcinoma can be improved by attention to laboratory procedures by the clinician.

Biopsy↗

Survey of the sputum cytology service in England and Wales.

Of the 231 laboratories carrying out sputum cytology in England and Wales, information on the staffing, laboratory procedures, workload, records, and results for the year 1971 was obtained from 228 (98.7%) by means of a questionnaire, which was completed in 1973 in 81% of the laboratories and at the end of 1972 in the remainder. At the time the questionnaire was completed, the number of laboratories per region offering a service ranged from 6 to 25. Most of the processing of specimens was done by technicians and non-medical screeners (technicians without recognized qualifications) and most of the microscopy by pathologists and technicians. Papanicolaou and/or haematoxylin and eosin stains were used in 97% of the laboratories. During 1971, the number of specimens tested per laboratory ranged from 9 to 6000. A median of 381 specimens and 165 patients were tested per laboratory. Almost all specimens were submitted by hospitals and chest clinics and only 1% by general practitioners. An estimated median of 6.5% of all cytological specimens were sputum, most of the remainder being cervical smears. Laboratories varied greatly in their positive (0%-24%) and doubtful positive (0%-27%) rates. An estimated 63 000 patients had their sputum tested in 1971, and about 6300 had positive reports, rather less than one-quarter of all patients diagnosed as having lung cancer in England and Wales in 1971. The cost of the service was estimated to be of the order of pound375 000 at 1974 prices; that is, pound2.50 per specimen or pound6.00 per patient tested. An improved service might best be provided by district general hospital laboratories testing at least 1000 specimens per year so that a high level of accuracy in reporting could be assurred. A large proportion of the microscopy might be done by non-medical graduates and technicians under the supervision of pathologists.

Cervix Uteri↗

The synergistic effect of sustained delivery of DHT, DHEA, and E on the ventral prostate of adult male rodents.

Previously, our laboratory has reported that tricalcium phosphate lysine drug delivery system (TCPL) can be used successfully to deliver dihydrotestosterone (DHT) and testosterone (T) at a sustained manner for long duration. The objective of this study was to evaluate the effects of estrogen (E), DHT and dehydroepiandrosterone (DHEA), delivered in a sustained manner, individually or in combination, by means of TCPL delivery system on the morphological changes of ventral prostatic tissue. Adult male rats (BW 300-350 gm) were randomly divided into four equal groups. Animals in Group I served as our intact unimplanted controls. Capsules implanted in Group II rats were loaded with TCPL containing 100 mg of DHEA. Rats in Group III were implanted with TCPL loaded with 100 mg of DHEA and 500 mg of DHT, while rats in Group IV were implanted with TCPL loaded with 100 mg of DHEA, 500 mg of DHT and 200 mg of E. Surgical aseptic techniques were performed according to standard laboratory procedures. The animals were maintained at the University of Mississippi Medical Center Animal Facilities following the rules and regulations established by NIH on the Care and Use of Laboratory Animals. At the end of 8 weeks post implantation, all animals were sacrificed and the prostatic tissues were collected, weighed and embedded for histopathological evaluations. Statistical analysis was conducted by using standard computer programs (STATEVIEW, JANDEL, ANOVA at 95% CI). The data obtained in this investigation demonstrated that exogenous intake of E + DHEA + DHT delivered in a sustained manner for eight weeks induced several pathophysiological conditions in ventral prostatic tissue in comparison to prostatic tissue collected from control animals. Cytopathological evaluations of tissue collected from Group II animals demonstrated the following: (i) atrophic pattern of the epithelium, (ii) small, round glands with low cuboidal epithelium, (iii) hypertrophy alone or in conjunction with occasional hyperplasia of the epithelial cells, and (iv) an increased presence of connective tissue stroma. In contrast, ventral prostate collected from animals in Group IV showed an increase in weights of the net prostatic tissues in comparison to the control group. Histopathological observations such as pleomorphism, low cuboidal to pseudostratified glands, prostatic hyperplasia of the epithelial cells, occasional mitotic activity, and occasional presence of connective tissue stroma were detected. In conclusion, the results of this study suggest that the use of TCPL capsules loaded with steroid hormones, individually or in combination can be used to regulate the growth and functional behavior of prostatic tissue in male rodents.

Animals↗

TCPL delivery system: the role of DHT, DHEA, and E on the epididymal tubules of adult male rats.

Recent studies reported from our laboratory have established that the sustained delivery of danazol in combination with androgens resulted in the remarkable reduction of epididymal mass. In addition, previous studies have recommended that ultrastructural of epididymal tubules have to be elucidated. The specific objective of this investigation was to evaluate the cytological characteristics of epididymal tissues exposed to sustained delivery of dihydrotestosterone (DHT), dehydroepiandrsterone (DHEA) and a combination of Estrogen (E), DHEA plus DHT by means of tricalcium phosphate lysine (TCPL) delivery system. Adult male rats (BW 300-350 gm) were randomly divided into four equal groups: Group I animals were implanted i.p. with TCPL loaded with DHEA (100 mg). Animals in group II were implanted with TCPL capsules loaded with DHEA (100 mg) + DHT (500 mg). Group III animals were implanted with TCPL capsules loaded with E (200 mg) + DHEA (100 mg) + DHT (500 mg). Group IV animals served as the intact unimplanted controls. Surgical aseptic techniques were performed according to standard laboratory procedures. The animals were maintained at the University of Mississippi Medical Center Animal Facilities following the rules and regulations established by NIH on the Care and Use of Laboratory animals. At the end of 6 weeks post implantation, all animals were sacrificed and the epididymal tissues were collected, weighed, and embedded for histopathological evaluations. Statistical analysis was conducted by using standard computer programs (STATVIEW, ANOVA at 95% CI). The data obtained in this investigation demonstrated the following: (1) remarkable reduction in sperm counts and motility obtained from epididymal tubules in all experimental (hormonally treated) groups, (2) the lumen of the epididymal tubules were devoid of sperm in animals treated with DHT in comparison to the control, (3) a decrease in the diameter of tubules with occasional hypertrophic epithelium in all experimental animals, (4) disorganization of nuclear material was observed in animals treated with DHEA and DHEA + E + DHT in comparison to the control group. The overall observation of this study suggests that sustained delivery of DHEA, DHEA + DHT, and DHEA + DHT + E can be used to regulate the structural and functional architecture of the site of extramaturation of spermatozoa.

Animals↗

Genital herpes simplex virus infections: current concepts in diagnosis, therapy, and prevention.

Genital herpes simplex virus infection is the commonest cause of genital ulcerations in persons in industrialized nations. Overlap in the clinical manifestations of genital herpes with other infectious and noninfectious causes of genital ulcerations often requires clinicians to seek laboratory confirmation of the cause of a genital ulcer. The sensitivity of any laboratory procedure for detecting herpes simplex virus infection differs with the stage of the disease. Isolation of the herpes simplex virus in tissue culture is the most sensitive and specific laboratory test for the confirmation of genital herpes; cytologic and antigen detection techniques are approximately 50% and 70% as sensitive as viral isolation. The antiviral compound acyclovir is the first medication useful for the management of persons with genital herpes. Prevention of transmission of infection and recurrences of disease, however, has not been achieved. Description of the clinical manifestations of disease, its variable recurrence rate, and potential for future infectivity are issues that physicians should discuss with patients who have genital herpes.

Acyclovir↗

Competing on the basis of fees?

The Department of Health, Education, and Welfare recently selected specific laboratory procedures for reimbursement under the lowest charge level criteria, mandated in Section 224 of the 1972 Social Security Amendment. According to the author, such government regulations could force hospital laboratories to compete with independent laboratories on the basis of fees, thereby threatening the former's financial stability. In this article, the author analyzes the problem and proposes a solution.

Fees and Charges↗

[Mandatory postgraduate medical training in Japan--present state of Nihon University as a private medical school].

This is a time of considerable uncertainty about the future of the postgraduate medical education policy of the Japanese government. Strong and visionary academic leadership of laboratory physicians in private medical schools is needed. The medical schools must not only adapt to a changing health care system, but also maintain excellence in education, patient care, and clinical research. In Japan, tradition has it that the comparatively few faculty members at national medical schools are mostly promoted only on the basis of research in experimental medicine, therefore, young medical graduates are increasingly drawn to bench work or molecular medicine, not to clinical practice. Single-minded specialization tends to produce single track minds, which may lack balanced judgment in approaching the appropriateness of both investigation and management. For continuity of care and containment of costs, a year or two of general professional training after graduation preceded by a broad medical education is an invaluable investment. All medical graduates, whatever their intended or unintended final destination (even if not clinical), should spend more than six months in medicine and four or five months in surgery, at least half of each to be spent in the general disciplines, including responsibilities for acute emergency admissions. As certified laboratory physicians we must attempt to attract graduates into laboratory medicine by developing imaginative training programs including common laboratory procedures such as Gram's stain, Wright-Giemsa stain and point of care testing at the patient's bedside or in ambulatory clinics, not only in central clinical laboratories.

Curriculum↗

[Qualitative microscopic evaluation of fecal fats. Comparative study].

Microscopical examination of stool fat is an easy laboratory procedure a long ago used as screening for steatorrhea. Quantitative analysis of stool fat is accepted as the best method to diagnose fat malabsorption, but stool collection and the laboratory technique are more difficult and time-consuming. The main objective of this work was performed to compare the accuracy of microscopical qualitative examination in the same fecal sample collected in 500 patients, 299 adults and 201 children, for the three days quantitative method. The diagnosis of steatorrhea was confirmed by chemical determination in 237 adults (stool fat values greater than 5 g) and 131 children (stool fat values greater than 2 g). From these 62 patients adults and 70 children were admitted as controls. The study showed that microscopical qualitative fat evaluation is highly comparable with findings obtained by chemical dosage. However, it is important to consider the use of two microscopic examination for each fecal sample, that must be carefully homogenized.

Adult↗