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Modelling risk factors for injuries from dog bites in Greece: a case-only design and analysis.

We conducted a study using a newly developed dataset based on Emergency Departments records of a network of hospitals from Greece on injuries from dog bites. Our goal is three-fold: (a) to investigate if surrogate factors of leisure time are associated with increased risk of injury from bites; (b) to address recently reported contradictory results on putative association of lunar periods and injuries from dog bites; and (c) to offer a general methodology for addressing similar case-only designs with combined factors of which some can exhibit cyclical patterns. To address these goals, we used a case-only design of our dataset, and conducted an analysis where we controlled simultaneously for weekday/weekend effects, season of year (winter, spring/fall, summer), and lunar periods, because any one of these factors can contribute to the degree of exposure to injuries from dog bites. We found that increased risk of injury from bites was associated with weekends versus weekdays (RR=1.19, 95% CI: 1.10-1.29), summer versus winter (RR=1.24, 95% CI: 1.11-1.39), and fall or spring versus winter (RR=1.31, 95% CI: 1.19-1.45). The results support the hypothesis that longer leisure time at these levels of factors does increase the risk of having a bite injury. Moreover, after controlling for these factors, risk of bite injury was not associated with moon periods, thereby also helping settle a longstanding argument.

Animals↗

Fatalities from non-use of seat belts and helmets in Greece: a nationwide appraisal. Hellenic Road Traffic Police.

It has been established that seat belt use by car occupants and helmet use by motorcycle riders substantially reduces the risk of serious and fatal injuries following accidents. No study, however, has evaluated the motor vehicle deaths that could be prevented in Greece by general use of these devices, even though this country has the highest mortality from motor vehicle accidents in the European Union. We have estimated the odds ratios (OR) for death rather than injury in a motor vehicle accident by seat belt use among occupants of passenger cars or helmet use among motorcycle riders, using a nationwide database in which persons killed or injured in road traffic accidents in 1985 and 1994 were recorded. The study base included 910 dead and 19,511 injured persons for 1985 and 1203 dead and 22,186 injured persons for 1994. The OR and 95% confidence intervals (CI) for death rather than injury was 0.69 (CI: 0.58 to 0.81, p < 10(-5)) for seat belt users versus non-users and 0.64 (CI: 0.51 to 0.81; p < 10(-3)) for helmet users versus non-users. There was evidence that the protective effect of these passive safety devices increased from 1985 to 1994 probably reflecting technological improvements. The proportion of all deaths that could have been avoided if all car occupants used seat belts was estimated to 27%, whereas 38% of motorcycle deaths could have been avoided if all motorcycle riders used helmets. These proportions translate to about 500 deaths per year, mostly deaths among young men.

Accidents, Traffic↗

Biomonitoring of Gulf of Patras, N. Peloponnesus, Greece. Application of a biomarker suite including evaluation of translation efficiency in Mytilus galloprovincialis cells.

Specimens of Mytilus galloprovincialis were placed in bow nets and immersed at 3-10 m depth in a clean coastal region (reference area), Itea, and two marine stations along Gulf of Patras, N. Peloponnesus, Greece. One site is near the estuaries of the Glafkos River, which are influenced by local industrial and urban sources (Station 1); the second site, Agios Vasilios, has no evident organic pollution but is enriched in metals, particularly zinc (Station 2). One month after immersion, digestive glands were removed from the mussels and tested for lysosomal membrane stability, metallothionein content, and translational efficiency of ribosomes. In addition, gill cells were isolated and their micronuclei content was determined. Compared with the reference samples, mussels transplanted to Gulf of Patras showed a significant increased lysosomal membrane permeability and metallothionein content, reduced polysome levels, and increased chromosomal damage in relation to the contamination burden of each sampling area. Also, runoff ribosomes from mussels transplanted to Gulf of Patras (that is, ribosomes stripped of endogenous messengers and peptidyl- or/and aminoacyl-tRNAs) were less efficient at initiating protein synthesis in an in vitro-translation system than those prepared from reference samples. The whole set of data suggests that the degree of Gulf of Patras pollution differs between different sites and depends on the proximity of urban sewage and industrial outfalls. In addition, our results emphasize the importance of protein synthesis regulation as a component of the cellular stress response.

Animals↗

Epidemiologic aspects of endometrial cancer in Thrace, Greece.

To describe the epidemiologic characteristics of endometrial cancer in relation with certain risk factors affecting the two major ethnic groups (Christian Orthodox and Muslims) in the area of Thrace, Greece. We performed a cross-sectional study of 166 consecutive patients, aged between 29 and 87 years, with documented endometrial cancer who were referred to our clinic for treatment from January 1986 to June 1998. Epidemiologic characteristics of the patients were abstracted from medical charts. To evaluate our results we used the direct standardization method (1995 Eur. Population) and chi2 test. The endometrial cancer incidence for the two study populations (Christian Orthodox and Muslims) was 16.96 and 3.16/100,000, respectively. We observed statistically significant differences in most epidemiologic characteristics between the two major ethnic groups in Thrace (Christian Orthodox and Muslims) regarding endometrial cancer. Our results confirm the increasing trend of endometrial cancer incidence as established in previous investigations.

Adult↗

Seasonal variation of gastrointestinal nematodes of sheep in the region of Joannina, Greece.

Parasitological and growth studies on two groups of naturally infected sheep, with or without anthelmintic treatment, from the age of 3 months to 2 years were carried out in the region of Joannina, Greece. A split-plot design was used so that each group, consisting of seven pure-bred Boutsiko and seven cross-bred Boutsiko with Karamaniko (F1) lambs, grazed separate parasitologically equivalent pasture plots. Faecal egg counts, pasture larval counts, plasma pepsinogen levels and live weight were recorded monthly. Infective larvae on each pasture plot increased during autumn and winter. Mean faecal egg counts for strongyle-type eggs were higher in the non-treated than the treated group and in the cross than the pure-bred sheep. Mean plasma pepsinogen levels were higher during autumn of the second year of the study. The results of the study suggest that the factors affecting the epidemiology of gastrointestinal nematodes of naturally infected sheep during grazing in the region of Joannina include anthelmintic treatment, host genotype and season, while the effectiveness of anthelmintic treatment in this study, as applied in the area, was questionable.

Age Factors↗

Application of solid-phase microextraction in the monitoring of priority pesticides in the Kalamas River (N.W. Greece).

A solid-phase microextraction (SPME) method was applied to an extended monitoring survey of priority pesticides for the European Union for a period of 12 months in water of the Kalamas River (Epirus region of northwestern Greece) in order to determine their concentrations and seasonal variations. Polydimethylsiloxane-coated fiber (100 microm) was used. The samples were screened using gas chromatography with flame thermionic detection. Detection was confirmed by gas chromatographymass spectroscopy. The most frequently detected pesticides were some of the more commonly used herbicides, such as S-ethyl-N,N-di-n-propylthiol carbamate (EPTC), trifluralin, atrazine, deethylatrazine, terbuthylazine and alachlor, and insecticides, such as carbofuran, diazinon, disulfoton, parathion methyl, parathion ethyl, fenthion and ethion. Concentrations of individual compounds ranged from 0.020 to 0.3 microg/L. Greater pesticide concentrations occurred during the seasons of application. A comparison with a well-established solid-phase extraction (C18 disks) procedure was performed for samples of high-season application (May-September) in order to confirm the effectiveness of the SPME technique. The results demonstrate the suitability of the SPME method for routine screening multiresidue analysis in natural waters.

Chromatography, Gas↗

Monitoring of pesticide residues and their metabolites in surface and underground waters of Imathia (N. Greece) by means of solid-phase extraction disks and gas chromatography.

Seasonal variations of pesticide residues in surface waters and ground waters of the Imathia area of Central Mecedonia (N. Greece) were determined for the period from May 1996 to April 1997. The sampling cruises included eight sites in rivers Aliakmon, Loudias, Tripotamos, Arapitsa and Canal-66, seven water springs in the mountain Vermion, seven rainfall water collection stations and one hundred underground points. Solid-phase extraction disks followed by gas chromatographic techniques with flame thermionic detection, electron capture detection and mass-selective detection were used for the monitoring of various pesticides their transformation products in environmental waters. The most commonly encountered pesticides in underground waters, were alachlor, atrazine, desethylatrazine (DEA), metolachlor, molinate, propanil, simazine, carbofuran, diazinon and parathion methyl. The above compounds including propazine, trifluralin, malathion, parathion ethyl, lindane, alpha-benzene hexachloride (alpha-BHC), beta-BHC, 4,4'-DDE and heptachlor were determined in river waters. The higher concentrations in underground waters were measured during the period from May to August, 1996, following seasonal application and diminished significantly during the autumn and winter. Water pollution by triazine and chloroacetanilides was highest in the estuarine areas; showing that many of these compounds are transported significant distances from their application sites. The major inputs of atrazine, alachlor, simazine and metolachlor occurred in May and June just after their application. Atrazine, DEA, diazinon and metolachlor were also detected in spring waters at concentration levels below 0.006 microgram/l. Finally, atrazine, DEA, carbofuran, simazine, diazinon, parathion ethyl and parathion methyl were detected in rainfall water samples collected in the agricultural area of Imathia (central part of the plain).

Agriculture↗

Essential oil analysis and antimicrobial activity of eight Stachys species from Greece.

The volatile composition of eight Stachys species has been studied. The investigated taxa were St. alopecuros (L.) Bentham., St. scardica (Griseb.) Hayek, St. cretica L. ssp. cretica, St. germanica L. ssp. heldreichii (Boiss.) Hayek, St. recta L., St. spinulosa L., St. euboica Rech. and St. menthifolia Vis., growing wild in Greece. The essential oils were obtained by hydrodistillation in a modified Clevenger-type apparatus, and their analyses were performed by GC and GC-MS. Identification of the substances was made by comparison of mass spectra and retention indices with literature records. Sesquiterpene hydrocarbons were shown to be the main group of constituents of all taxa. Furthermore, the obtained essential oils were tested against the following six bacteria: Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 35210), Bacillus subtilis (ATCC 10907), Bacillus cereus (clinical isolates), Micrococcus flavus (ATCC 10240), Staphylococcus epidermidis (ATCC 2228), as well as against the following five fungi: Aspergillus niger (ATCC 6275), Penicillium ochrochloron (ATCC 9112), Epidermophyton floccosum (clinical isolates), Candida albicans (clinical isolates) and Trichophyton mentagrophytes (clinical isolates). The tested essential oils showed better activity against bacterial species than against fungi. Pseudomonas aeruginosa was the most resistant strain, as none of the essential oils was active against this strain. The essential oil of St. scardica has been proven most active against both bacteria and fungi.

Anti-Infective Agents↗

Comparison of the pathogenic, antigenic and molecular characteristics of two encephalomyocarditis virus (EMCV) isolates from Belgium and Greece.

The pathogenicity of two porcine encephalomyocarditis virus (EMCV) isolates for sows in gestation and young piglets was studied. One virus originated from a case of reproductive failure in pigs in Belgium and the other from a case of acute myocarditis in pigs in Greece. Sows in the mid-gestation period and one- to two-month old piglets were inoculated with each isolate. The molecular relationship between both isolates was studied by determining the nucleotide sequence located across the junction of the 1C and 1D capsid-coding genes. Antigenic analysis was performed using a panel of 35 monoclonal antibodies raised against an Italian field isolate of EMCV. All three approaches revealed differences between both isolates and also confirmed that there was no link between the two outbreaks of disease.

Animals↗

Removal and recovery of p-phenylenediamines developing compounds from photofinishing lab-washwater using clinoptilolite tuffs from Greece.

Clinoptilolite tuffs from areas in Thrace region of Greece are compared with synthetic zeolites NaY and NH4Y for the uptake of N4-ethyl-N4-(2-methansulphonamidoethyl)-2-methyl-1,4-phenylenediamin (sesquisulphate, monohydrate) with the trade name CD-3 for the purpose to be used for clean-up and recycling photo-finishing and photo-developing washwaters. The cation-exchange capacity is found to be 6.15-11.1 mg/g for zeoliferous tuffs at equilibrium concentration of 50 ppm CD-3 in aqueous solution compared to 65.0 mg/g of NaY and 48.2 mg/g for NH4Y synthetic zeolites corresponding to the removal of CD-3 from 120 to 2001 of 50 ppm aqueous solution per kg of natural zeoliferous tuff; this capacity is only 6-10 times lower than type-Y synthetic zeolite. Initial rates of uptake are 20.8 mg/l/min for natural and 38.5 mg/l/min for synthetic zeolites. Regeneration levels of 55, 23, 35, and 33% are obtained for MCH, SF, NaY, and NH4Y, respectively. The rapid and almost complete uptake of CD-3 from its aqueous solutions at low CD-3 concentrations by the natural zeolites is promising for such an application.

Aniline Compounds↗

Assessment of the surface water quality in Northern Greece.

The application of different multivariate statistical approaches for the interpretation of a large and complex data matrix obtained during a monitoring program of surface waters in Northern Greece is presented in this study. The dataset consists of analytical results from a 3-yr survey conducted in the major river systems (Aliakmon, Axios, Gallikos, Loudias and Strymon) as well as streams, tributaries and ditches. Twenty-seven parameters have been monitored on 25 key sampling sites on monthly basis (total of 22,350 observations). The dataset was treated using cluster analysis (CA), principal component analysis and multiple regression analysis on principal components. CA showed four different groups of similarity between the sampling sites reflecting the different physicochemical characteristics and pollution levels of the studied water systems. Six latent factors were identified as responsible for the data structure explaining 90% of the total variance of the dataset and are conditionally named organic, nutrient, physicochemical, weathering, soil-leaching and toxic-anthropogenic factors. A multivariate receptor model was also applied for source apportionment estimating the contribution of identified sources to the concentration of the physicochemical parameters. This study presents the necessity and usefulness of multivariate statistical assessment of large and complex databases in order to get better information about the quality of surface water, the design of sampling and analytical protocols and the effective pollution control/management of the surface waters.

Environmental Monitoring↗

Polycyclic aromatic hydrocarbons in the bulk precipitation and surface waters of Northern Greece.

Bulk (wet and dry) precipitation and surface water sampling was undertaken in the main plain of central Macedonia in Northern Greece. Fourteen polycyclic aromatic hydrocarbons (PAHs) included in the US EPA's priority pollutant list were analysed. The concentrations determined in bulk precipitation were in general within the range of values worldwide reported. Concentrations were highest in the cold months. Deposition fluxes of PAHs were of the same order of magnitude as reported data. The greatest values were found when high concentrations of PAHs in precipitation coincided with large precipitation amounts. The concentrations of PAHs in surface waters (main rivers, tributaries, ditches, etc) were in general lower than those in bulk precipitation, and among the lowest reported for European rivers, excepting Np and Ph. Bulk deposition and domestic effluents are suggested as being the main PAH sources into surface waters.

Cold Temperature↗

Phosphorus fractionation in lake sediments--lakes Volvi and Koronia, N. Greece.

Sediments from two lakes, the meso-to-eutrophic Volvi and the hypertrophic Koronia, located in N. Greece were examined on the basis of P-fractionation. In both lakes, the rank order of P-fractions was HCl-P > NaOH-P > BD-P > NH4Cl-P. The loosely sorbed phosphorus (NH4Cl-P) represented < 1% of the sedimentary inorganic phosphorus, while the reductant phosphorus (BD-P) ranged 5-6%. The calcium bound phosphorus (HCl-P) showed considerable contribution (59-74%) to the sedimentary inorganic P-loads. The metal oxide bound phosphorus (NaOH-P) was higher in the hypertrophic (30-35%) than in the meso-to-eutrophic system (19-28%). Fine-sized sediments exhibited significantly higher concentrations of HCl-P in Volvi and NH4Cl-P in Koronia. Sampling month had significant effect in variance of most P-fractions and other sediment features in both lakes. Use was also made of multivariate statistics to identify the factors which influence the sedimentary phosphorus. NaOH-P was the most reactive fraction in Lake Volvi. Iron compounds and organic matter seem to play a significant role in regulating this labile P-budget. NH4Cl-P was the more reactive fraction in Lake Koronia which was influenced by sedimentation of P-absorbed on clay/silt fine particles.

Calcium↗

PCDD/Fs and PCBs in airborne particulate matter of the greater Thessaloniki area, N. Greece.

Particle-bound polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) were monitored at two sites in northern Greece for an eight-month period in 1999. PCDD/F concentrations were close to the lower end of reported values worldwide. Excepting a few cases, the PCDD/Fs homologue profile was stable. The gaseous PCDD/F fractions calculated were found to account for a small percentage of the total concentrations (<2% for OCDD/Fs and HpCDD/Fs, while 30-35% for TCDFs). Particle-bound PCBs were also found at low concentrations which, however, were higher at the urban site. Calculations of the dry deposition of particulate PCDD/ Fs and PCBs gave mean values of 0.52 and 0.59 pg I-TEQ/m2/day of PCDD/Fs, while 242 and 74 pg/m2/day of sigmaPCBs for the urban and the semirural areas respectively. An anticorrelation of PCDD/F concentrations with ambient temperature was derived particularly for the lower chlorinated congeners. A weak association with winds of western and southern origin was also observed. Factor analysis and literature source profiles were employed to identify possible emission sources. It was appeared that the PCDD/F compositional pattern of TSP is influenced by mixed sources the most prominent being uncontrolled fires and car exhausts.

Air Movements↗

Toxicity and heavy metal contamination of surficial sediments from the Bay of Thessaloniki (Northwestern Aegean Sea) Greece.

Surficial sediments (the fraction < 2000 microm) from the Bay of Thessaloniki, in the Northwestern Aegean Sea, Greece were examined for heavy metal and organic carbon contents, as well as for acute LUMIStox toxicity of pore waters (PWs), wet sediment elutriates (WSEs) and dry sediment elutriates (DSEs) obtained from the solid material remaining after PW extraction. WSEs where not toxic. EC20/50 values could be measured in some DSE and PW samples. In all sediment samples, the DSE toxicity was greater than the corresponding PW toxicity. Sediment concentrations of total and labile Cd, Pb, Cu, Cr, Zn, Mn, Ni and Fe were determined and evaluated in relation to sediment quality guidelines. Pollution levels ranged from low to high for certain metals. A misfit between sediment toxicities calculated from heavy metal concentrations and those biologically measured was observed. Toxicity values were in general poorly correlated with sediment's heavy metal or organic carbon content. As significant correlation was however found between the DSE toxicity with total Ni and labile Fe, as well as for the PW toxicity with total Ni, labile Fe and labile Cr.

Carbon↗

Organochlorine pesticides in the surface waters of Northern Greece.

A survey undertaken in Northern Greece has shown that organochlorine pesticides are present in the surface waters. Surface water samples have been collected seasonally from four rivers and five lakes for a period of two years. Solid-phase extraction followed by gas chromatographic techniques with electron capture detection was used for the determination of the compounds. The most commonly encountered organochlorine pesticides in surface waters were the isomers of hexachlorocyclohexane, aldrin, dieldrin and endosulfan sulfate. In some cases the concentrations detected were higher than the qualitative target levels set by the European Union, especially for hexachlorocyclohexane and aldrin. The occurrence of these compounds in Greek surface waters can be attributed to intense agricultural activity as well as to transboundary pollution.

Agriculture↗

Determination of polar pharmaceuticals in sewage water of Greece by gas chromatography-mass spectrometry.

Sewage influents and effluents of different urban areas of Greece, were analyzed for polar pharmaceutical residues, used in human medicine. Drugs investigated were the anti-inflammatory drugs diclofenac and ibuprofen, the metabolite of the drugs clofibrates used as blood lipid regulators, clofibric acid and the analgesics phenazone and propyphenazone. Analysis was carried out using capillary gas chromatography-mass spectrometry with selected ion monitoring. The method used was involved solid phase extraction (C(18)) and derivatization with pentafluorobenzyl bromide. Diclofenac was detected in every sewage effluent sample.

Gas Chromatography-Mass Spectrometry↗

Volatile organic compounds in the surface waters of northern Greece.

An investigation into the occurrence of volatile organic compounds (VOCs) was conducted for a period of two years in the surface waters of Northern Greece. Samples from four rivers and five lakes were taken seasonally and analyzed for VOCs. The analysis has been performed by purge-and-trap (PAT) gas chromatographic-mass spectrometric (GC-MS) technique. The most commonly encountered VOCs in surface waters were chloroform, carbon tetrachloride, trichloroethylene, dichlorobromomethane, tetrachloroethylene, and chlorodibromomethane.

Carbon Tetrachloride↗