Microinjection of BAC DNA into the pronuclei of fertilized mouse oocytes.
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Animals↗
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Generation of BAC transgenic mice.
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Animals↗
BAC rescue: a tool for functional analysis of the mouse genome.
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Animals↗
Construction of HSV-1 BACs and their use for packaging of HSV-1-based amplicon vectors.
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Animals↗
Mutagenesis of viral BACs with linear PCR fragments (ET recombination).
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Bacteriophage lambda↗
Mutagenesis of herpesvirus BACs by allele replacement.
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Alleles↗
Protective DNA vaccination by particle bombardment using BAC DNA containing a replication-competent, packaging-defective genome of herpes simplex virus type I.
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Animals↗
Use of Nipponbare BAC clones for physical mapping of an R gene locus in rice.
Major advances in rice genomics during the last few years have made positional cloning in rice much more efficient. Nipponbare is a model rice genotype being sequenced by the International Rice Genome Sequencing Project Consortium. Here, we describe an efficient procedure of the construction of physical map for positional cloning of resistance gene (R) using the Nipponbare genetic resources. This advanced strategy should be useful for the efficient identification of agronomic important R genes from many resistant rice genotypes, including wild rice species.
Chromosomes, Artificial, Bacterial↗
Age and BAC when arrested for drunken driving and public drunkenness.
Blood alcohol concentrations of young people arrested for driving while intoxicated and public drunkenness over a 4-year period in Randolph County, Arkansas, tended to be lower than those of older arrestees.
Adolescent↗
Hybridization to high-density filter arrays of a Brugia malayi BAC library with biotinylated oligonucleotides and PCR products.
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Animals↗
Isolation and subcloning of large fragments from BACs and PACs.
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Chromosomes, Artificial, Bacterial↗
Rapid localization of a gene within BACs and PACs.
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Ampicillin Resistance↗
BAC and PAC DNA for the generation of transgenic animals.
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Animals↗
Enhanced efficiency of pSV1-RecA-based BAC recombineering.
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Animals↗
Retrofitting bacterial artificial chromosomes by homologous recombination into enhanced BAC vectors for functional studies.
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Chromosomes, Artificial, Bacterial↗
Co-targeting a selectable marker to the Escherichia coli chromosome improves the recovery rate for mutations induced in BAC clones by homologous recombination.
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Chromosomes, Artificial, Bacterial↗
Zero-background plasmid vector for BAC library construction.
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Bacillus↗