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Nutrient cycling in a tropical seasonal rain forest of Xishuangbanna, Southwest China. Part 1: tree species: nutrient distribution and uptake.

Tropical rain forests are characterized by large numbers of the species with diverse growth habits. The objective of the present study was to determine the distribution of nutrient content in the major trees of the tropical rain forests in Xishuangbanna. This will improve the understanding of the nutrient losses from such sites that result from harvesting and flow of nutrients within the ecosystem and lead to the development of effective and rational forest management strategies. Based on the results in this study, the distribution of nutrients among biomass components of trees varied: The ordering of major elements concentrations was K > N > Mg > Ca > P in branch, stem and root tissues but was N > K > Mg > Ca > P in leaves. The maximum amount of all nutrients per ha occurred in the stems followed by branches, roots and leaves. Of the total uptake of 6167.7 kg ha(-1) of all nutrients, the contribution of various nutrients was found to be N (2010.6 t ha(-1)), P (196.3 t ha(-1)), K (2123.8 kg ha(-1)), Ca (832 kg ha(-1)) and Mg (1005 kg ha(-1)). However, comparing the nutrient uptake of other tropical and sub tropical forests, the results indicated that rates for the Xishuangbanna forests were 20-35% lower than previously reported values.

Biomass↗

Random forest: a classification and regression tool for compound classification and QSAR modeling.

A new classification and regression tool, Random Forest, is introduced and investigated for predicting a compound's quantitative or categorical biological activity based on a quantitative description of the compound's molecular structure. Random Forest is an ensemble of unpruned classification or regression trees created by using bootstrap samples of the training data and random feature selection in tree induction. Prediction is made by aggregating (majority vote or averaging) the predictions of the ensemble. We built predictive models for six cheminformatics data sets. Our analysis demonstrates that Random Forest is a powerful tool capable of delivering performance that is among the most accurate methods to date. We also present three additional features of Random Forest: built-in performance assessment, a measure of relative importance of descriptors, and a measure of compound similarity that is weighted by the relative importance of descriptors. It is the combination of relatively high prediction accuracy and its collection of desired features that makes Random Forest uniquely suited for modeling in cheminformatics.

Journal Article↗

Forestry practices increase mercury and methyl mercury output from boreal forest catchments.

We observed significant increases in the runoff output of total mercury (TotHg) and methyl mercury (MeHg) from a small spruce forest catchment (0.071 km2) after clear-cutting and soil treatment. Here we show that forest regeneration practices may act as an important additional source of TotHg and MeHg to forest lakes. TotHg and MeHg in runoff from two small forested catchments were monitored during the period 1994 to 2001. In the autumn of 1997, one of the catchments was clear-cut Soil preparation (mounding) was carried out in the autumn of 1998 and replanting in the summer of 1999. During the 3 years after the silvicultural treatment, medians of monthly flow-weighted TotHg and MeHg concentrations (12.02 ng L(-1) and 0.35 ng L(-1), respectively) and output loads (0.80-0.97 g km(-2) a(-1) and 0.011-0.036 g km(-2) a(-1), respectively) increased significantly compared to the 3 years calibration period (8.13 ng L(-1) and 0.15 ng 1(-1); 2.0-5.3 g km(-2) a(-1) and 0.11-0.16 g km(-2) a(-1), respectively). These results indicate that clear-cutting and/or soil treatment significantly increases the mobility of TotHg and MeHg accumulated in forest soil and may thus be an important factor for the total input of Hg to boreal freshwater ecosystems.

Ecosystem↗

Impact of the 2002 Canadian forest fires on particulate matter air quality in Baltimore city.

With increasing evidence of adverse health effects associated with particulate matter (PM), the exposure impact of natural sources, such as forest fires, has substantial public health relevance. In addition to the threat to nearby communities, pollutants released from forest fires can travel thousands of kilometers to heavily populated urban areas. There was a dramatic increase in forest fire activity in the province of Quebec, Canada, during July 2002. The transport of PM released from these forest fires was examined using a combination of a moderate-resolution imaging spectroradiometer satellite image, back-trajectories using a hybrid single-particle Lagrangian integrated trajectory, and local light detection and ranging measurements. Time- and size-resolved PM was evaluated at three ambient and four indoor measurement sites using a combination of direct reading instruments (laser, time-of-flight aerosol spectrometer, nephelometer, and an oscillating microbalance). The transport and monitoring results consistently identified a forest fire related PM episode in Baltimore that occurred the first weekend of July 2002 and resulted in as much as a 30-fold increase in ambientfine PM. On the basis of tapered element oscillating microbalance measurements, the 24 h PM25 concentration reached 86 microg/m3 on July 7, 2002, exceeding the 24 h national ambient air quality standard. The episode was primarily comprised of particles less than 2.5 microm in aerodynamic diameter, highlighting the preferential transport of the fraction of PM that is of greatest health concern. Penetration of the ambient episode indoors was efficient (median indoor-to-outdoor ratio 0.91) such that the high ambient levels were similarly experienced indoors. These results are significant in demonstrating the impact of a natural source thousands of kilometers away on ambient levels of and potential exposures to air pollution within an urban center. This research highlights the significance of transboundary air pollution and the need for studies that assess the public health impacts associated with such sources and transport processes.

Air Movements↗

A comparison of the ability of forest and agricultural soils to mineralize chlorinated aromatic compounds.

Soils were sampled from two agricultural fields, two relatively pristine forests, and one suburban forest in Ontario, Canada. The ability of these soils to mineralize 2,4-dichlorophenoxyacetate, 3-chlorobenzoate, 4-chlorophenol, 2,4-dichlorophenol, pentachlorophenol, and atrazine was determined using 14C-labeled substrates. Direct pre-exposure was necessary before atrazine mineralization could be detected; however, it was not necessary for degradation of any of the other chemicals. 2,4-dichlorophenoxyacetate and pentachlorophenol mineralization was much higher in the agricultural soils relative to the pristine forest soils, but 3-chlorobenzoate and 2,4-dichlorophenol mineralization rates showed the opposite trend. Mineralization of 4-chlorophenol was about equivalent in all soils. Suburban forests soils were indistinguishable from agricultural soils with respect to their degradation of 2,4-dichlorophenoxyacetate and chlorobenzoate. Additionally, they were better able than any of the soils to withstand the toxic effects of pentachlorophenol. Pentachlorophenol mineralization was highly variable in the pristine forest soils, ranging from about 6 to 50%. Abiotic factors such as pH, soil type, and organic and moisture content did not account for these significant site differences. The selective forces responsible for these differences, and the possible differences in microbial populations are discussed.

Agriculture↗

Rational forest productivity decline.

A whole forest optimisation model was employed to examine economic behaviour as it relates to long term, forest productivity decline in the boreal forests of Ontario, Canada. Our productivity investment model (PIM) incorporated a choice between productivity decline as represented by a drop in forest Site Class, and a fee to 'maintain' site productivity. Sensitivity analysis was used to determine the point at which these fees exceeded the value of the differential in timber volume between upper and lower site classes. By varying discount rate, 'productivity investment frontiers' were constructed, which highlight the effects of the magnitude in productivity decline, maintenance fees, and harvest flow constraints upon the occurrence and schedule of productivity declines. In presenting this simple approach to exploring the effects of economic choice upon forest productivity decline, the phenomena of 'natural capital divestment' within forestry is described.

Conservation of Natural Resources↗

A DNA sequence specific for forest form Onchocerca volvulus.

Onchocerciasis, or river blindness, is caused by infection with Onchocerca volvulus, a filarial parasite which infects about 40 million people in Africa and Latin America. Epidemiological, clinical, entomological and serological studies of African onchocerciasis led to the hypothesis that Onchocerca volvulus exists in different forms in the forest and savannah. It is uncertain if these differences are due to genetic differences within O. volvulus itself, or to epigenetic factors, such as differences in the host populations. To date no basic biochemical differences between the forest and savannah populations of O. volvulus has been found, although isoenzyme studies have shown that differences in allele frequency between forest and savannah populations exist. Here we describe the isolation of a DNA sequence that seems to be specific for the forest form of O. volvulus, the first indication of a basic genetic difference between the savannah and forest forms.

Base Sequence↗

Tree species impoverishment and the future flora of the Atlantic forest of northeast Brazil.

Estimates of species extinction due to human impact on tropical forests have previously been based on the relationship between species number and area. Here we use a different approach to estimate loss of tree species in the Atlantic forest of northeast Brazil. We evaluate the characteristics of plant species, their avian dispersers and the distribution of the forest remnants on the landscape to estimate that about 33.9% of tree species in this region will become extinct on a regional scale. Because northeast Brazil is the most threatened sector of South American Atlantic forest, our results highlight the need to change the current conservation paradigm for this region. Rather than focus on the creation of isolated reserves in any medium-to-large forest remnant, a bioregional planning approach is urgently required to rescue this unique biota from extinction.

Animals↗

Respiration as the main determinant of carbon balance in European forests.

Carbon exchange between the terrestrial biosphere and the atmosphere is one of the key processes that need to be assessed in the context of the Kyoto Protocol. Several studies suggest that the terrestrial biosphere is gaining carbon, but these estimates are obtained primarily by indirect methods, and the factors that control terrestrial carbon exchange, its magnitude and primary locations, are under debate. Here we present data of net ecosystem carbon exchange, collected between 1996 and 1998 from 15 European forests, which confirm that many European forest ecosystems act as carbon sinks. The annual carbon balances range from an uptake of 6.6 tonnes of carbon per hectare per year to a release of nearly 1 t C ha(-1) yr(-1), with a large variability between forests. The data show a significant increase of carbon uptake with decreasing latitude, whereas the gross primary production seems to be largely independent of latitude. Our observations indicate that, in general, ecosystem respiration determines net ecosystem carbon exchange. Also, for an accurate assessment of the carbon balance in a particular forest ecosystem, remote sensing of the normalized difference vegetation index or estimates based on forest inventories may not be sufficient.

Atmosphere↗

Limited carbon storage in soil and litter of experimental forest plots under increased atmospheric CO2.

The current rise in atmospheric CO2 concentration is thought to be mitigated in part by carbon sequestration within forest ecosystems, where carbon can be stored in vegetation or soils. The storage of carbon in soils is determined by the fraction that is sequestered in persistent organic materials, such as humus. In experimental forest plots of loblolly pine (Pinus taeda) exposed to high CO2 concentrations, nearly half of the carbon uptake is allocated to short-lived tissues, largely foliage. These tissues fall to the ground and decompose, normally contributing only a small portion of their carbon content to refractory soil humic materials. Such findings call into question the role of soils as long-term carbon sinks, and show the need for a better understanding of carbon cycling in forest soils. Here we report a significant accumulation of carbon in the litter layer of experimental forest plots after three years of growth at increased CO2 concentrations (565 microl l(-1)). But fast turnover times of organic carbon in the litter layer (of about three years) appear to constrain the potential size of this carbon sink. Given the observation that carbon accumulation in the deeper mineral soil layers was absent, we suggest that significant, long-term net carbon sequestration in forest soils is unlikely.

Atmosphere↗

Soil fertility limits carbon sequestration by forest ecosystems in a CO2-enriched atmosphere.

Northern mid-latitude forests are a large terrestrial carbon sink. Ignoring nutrient limitations, large increases in carbon sequestration from carbon dioxide (CO2) fertilization are expected in these forests. Yet, forests are usually relegated to sites of moderate to poor fertility, where tree growth is often limited by nutrient supply, in particular nitrogen. Here we present evidence that estimates of increases in carbon sequestration of forests, which is expected to partially compensate for increasing CO2 in the atmosphere, are unduly optimistic. In two forest experiments on maturing pines exposed to elevated atmospheric CO2, the CO2-induced biomass carbon increment without added nutrients was undetectable at a nutritionally poor site, and the stimulation at a nutritionally moderate site was transient, stabilizing at a marginal gain after three years. However, a large synergistic gain from higher CO2 and nutrients was detected with nutrients added. This gain was even larger at the poor site (threefold higher than the expected additive effect) than at the moderate site (twofold higher). Thus, fertility can restrain the response of wood carbon sequestration to increased atmospheric CO2. Assessment of future carbon sequestration should consider the limitations imposed by soil fertility, as well as interactions with nitrogen deposition.

Atmosphere↗

Increasing dominance of large lianas in Amazonian forests.

Ecological orthodoxy suggests that old-growth forests should be close to dynamic equilibrium, but this view has been challenged by recent findings that neotropical forests are accumulating carbon and biomass, possibly in response to the increasing atmospheric concentrations of carbon dioxide. However, it is unclear whether the recent increase in tree biomass has been accompanied by a shift in community composition. Such changes could reduce or enhance the carbon storage potential of old-growth forests in the long term. Here we show that non-fragmented Amazon forests are experiencing a concerted increase in the density, basal area and mean size of woody climbing plants (lianas). Over the last two decades of the twentieth century the dominance of large lianas relative to trees has increased by 1.7-4.6% a year. Lianas enhance tree mortality and suppress tree growth, so their rapid increase implies that the tropical terrestrial carbon sink may shut down sooner than current models suggest. Predictions of future tropical carbon fluxes will need to account for the changing composition and dynamics of supposedly undisturbed forests.

Analysis of Variance↗

An inventory-based carbon budget for forest and woodland ecosystems of Turkey.

Environmental monitoring of national-level comparisons of CO(2) emissions is needed to quantify sources and sinks of carbon (C) in national ecosystems. In this study, a national forest inventory database was used to estimate the past and current pools and fluxes of C in deciduous and coniferous forest and woodland ecosystems (20.7 x 10(6) ha) of Turkey. Growing C stock was 12.63 t C ha(-1) in 1960 and 16.55 t C ha(-1) in 1995. Total C store in the whole live woody biomass was estimated at 22.77 t C ha(-1) in 1996. The total flux of C from the atmosphere into the forest and woodland ecosystems driven by primary productivity was about 1.46 t C ha(-1)(or 30.2 Mt C) in 1996. The estimated net release of C from the forest and woodland ecosystems of Turkey to the atmosphere was about 1.34 t C ha(-1)(or 21.5 Mt C) in 1996. When C released was taken into account, net ecosystem sequestration (NES) resulted in 0.12 t C ha(-1) per year. Such analytical tools as national forest C budgets are needed to improve our preventive and mitigative strategies for dealing with global climate change.

Atmosphere↗

Fragmented forests, evolving flies: molecular variation in African populations of Drosophila teissieri.

Microsatellite variation from eight loci was studied in five populations of Drosophila teissieri, a fruit-fly found only in the rain forests of sub-Saharan Africa. Five noncontiguous rain forest sites (from Tanzania, Gabon and Ivory Coast) were sampled to measure the effects of historical forest fragmentation on population structure in an obligatory forest-dwelling species. The Ivory Coast and Gabon populations showed a wider range of alleles, different modal alleles and had a higher genetic diversity than the three East African populations. As could be expected, genetic differentiation (FST) was significantly correlated with physical distance, but the westernmost population (Ivory Coast) showed values that were intermediate between the central (Gabon) and Eastern (Tanzania) populations. A migration-drift equilibrium in a stable continuum of populations did not appear adequate to describe the observed distribution. It seems probable that the species has undergone abrupt changes involving isolation, merging and migration of populations, as a consequence of repeated waves of forest fragmentation and coalescence.

Africa↗

Genetic structure of the forest herb Primula elatior in a changing landscape.

To investigate whether changes in land use and associated forest patch turnover affected genetic diversity and structure of the forest herb Primula elatior, historical data on landscape changes were combined with a population genetic analysis using dominant amplified fragment length polymorphism markers. Based on nine topographic maps, landscape history was reconstructed and forest patches were assigned to two age classes: young (less than 35 years) and old (more than 35 years). The level of differentiation among Primula populations in recently established patches was compared with the level of differentiation among populations in older patches. Genetic diversity was independent of population size (P > 0.05). Most genetic variation was present within populations. Within-population diversity levels tended to be higher for populations located in older forests compared with those for populations located in young forests (Hj = 0.297 and 0.285, respectively). Total gene diversity was also higher for old than for young populations (Ht = 0.2987 and 0.2828, respectively). The global fixation index FST averaged over loci was low, but significant. Populations in older patches were significantly more differentiated from each other than were populations in recently established patches and they showed significant isolation by distance. In contrast, no significant correlations between pairwise geographical distance and FST were found for populations in recently established patches. The location of young and old populations in the studied system and altered gene flow because of increased population density and decreased inter-patch distances between extant populations may explain the observed lower genetic differentiation in the younger populations. This study exemplifies the importance of incorporating data on historical landscape changes in population genetic research at the landscape scale.

Age Factors↗

Climate change reverses the competitive balance of ash and beech seedlings under simulated forest conditions.

This study identifies the important role of climate change and photosynthetic photon flux density (PPFD) in the regenerative competence of ash and beech seedlings in 12 inter- and intra-specific competition designs in simulated mixed ash-beech forest gaps under conditions of non-limiting soil volume, water and nutrient supply. The growth conditions simulated natural forest conditions as closely as possible. Simulations were performed by growing interacting seedling canopies for one season in temperature-regulated closed-top chambers (CTCs). Eight CTCs were used in a factorial design with replicate treatments of [CO2] x temperature x PPFD x competition design. [CO2] tracked ambient levels or was 360 micromol mol-1 higher. Temperature tracked ambient levels or was 2.8 degrees C higher. PPFD on two plant tables inside each CTC was 16% and 5% of open-field levels, respectively, representative of typical light flux levels in a natural forest gap. In several of the competition designs, climate change made the ash seedlings grow taller than the beech seedlings and, at the same time, attain a larger leaf area and a larger total biomass. Advantages of this type for ash were found particularly at lower PPFD. There was a positive synergistic interaction of elevated temperature x [CO2] for both species, but more so for ash. There are many uncertainties when a study of chambered seedlings is to be projected to real changes in natural forests. Nevertheless, this study supports a possible future shift towards ash in north European, unmanaged, mixed ash-beech forests in response to the predicted climate change.

Carbon Dioxide↗

Variability in fruit and seed morphology among and within populations of Plathymenia (Leguminosae-Mimosoideae) in areas of the Cerrado, the Atlantic forest, and transitional sites.

Plathymenia reticulata is a tree species that occurs in two different Brazilian biomes, the Cerrado (a savannah environment), and the Atlantic Forest. In the present study, we evaluated morphological variation within and among five populations located in these vegetation types and in transitional sites in order to test the hypothesis that habitat selective pressures, being different in the Cerrado and the Atlantic Forest, would cause adaptive differences in morphological traits in individuals occurring under these different circumstances. Thirteen morphological traits of fruits, seeds, and of the membranous endocarp were obtained from 30 fruits and 20 seeds from each of nine to 10 individuals per population. Significant variation was found for all traits while comparing individuals within populations, and most traits varied significantly among populations as well. Some traits differed significantly between forest and Cerrado populations, while transition sites showed intermediate patterns and higher within-population variation. Contrary to our hypothesis, variation in seed size and mass among populations from different habitats was not significant. However, as predicted, the membranous endocarp was shorter for the seeds from Cerrado populations than from the Atlantic Forest. These data suggest the existence of P. reticulata ecotypes from Cerrado and Atlantic Forest; the relevance to Plathymenia evolution and to its wide ecological distribution is discussed.

Brazil↗

Economic value of tropical forest to coffee production.

Can economic forces be harnessed for biodiversity conservation? The answer hinges on characterizing the value of nature, a tricky business from biophysical, socioeconomic, and ethical perspectives. Although the societal benefits of native ecosystems are clearly immense, they remain largely unquantified for all but a few services. Here, we estimate the value of tropical forest in supplying pollination services to agriculture. We focus on coffee because it is one of the world's most valuable export commodities and is grown in many of the world's most biodiverse regions. Using pollination experiments along replicated distance gradients, we found that forest-based pollinators increased coffee yields by 20% within approximately 1 km of forest. Pollination also improved coffee quality near forest by reducing the frequency of "peaberries" (i.e., small misshapen seeds) by 27%. During 2000-2003, pollination services from two forest fragments (46 and 111 hectares) translated into approximately 60,000 USD per year for one Costa Rican farm. This value is commensurate with expected revenues from competing land uses and far exceeds current conservation incentive payments. Conservation investments in human-dominated landscapes can therefore yield double benefits: for biodiversity and agriculture.

Animals↗