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PubMed1997

Yeast gene YOR137c is involved in the activation of the yeast plasma membrane H+-ATPase by glucose.

Glucose triggers transcriptional and post-transcriptional mechanisms that increase the level and activity of Saccharomyces cerevisiae plasma membrane H+-ATPase. We have studied the post-transcriptional activation of the enzyme by glucose and have found that the YOR137c gene product is implicated in this activation. Deletion of YOR137c does not affect the level of Pma1 at the plasma membrane, but disturbs the glucose-triggered Vmax increase of the enzyme. We propose that at least two independent mechanisms are involved in glucose activation of the H+-ATPase.

Cell Membrane↗
PubMed1983

Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast.

Expression of the URA3 gene of Saccharomyces cerevisiae was studied by analysis of URA3-lacZ gene fusions constructed in vitro. Synthesis of hybrid beta-galactosidase by fusions in frame with the coding sequence for orotidine-5'-phosphate decarboxylase (OMPdecarboxylase) was found to be normally regulated even when only 11 nucleotides of URA3 coding sequence remained, indicating that all transcription initiation and regulatory sites are present at the beginning of the URA3 gene. An upstream initiator codon that begins a short overlapping coding sequence in another reading frame was also found to be active in producing hybrid beta-galactosidase. However this beta-galactosidase synthesis showed little or no regulation. Nuclease protection experiments revealed numerous species of URA3 mRNA. The regulation of these is consistent with the idea that the URA3 protein and the overlapping peptide are translated from differentially regulated mRNAs of different lengths.

Base Sequence↗
PubMedDate not supplied

Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila.

The HO gene of Saccharomyces cerevisiae encodes the endonuclease that initiates mating-type switching. To prevent inopportune switching, HO transcription is restricted to a specific period in the haploid cell cycle, which is just after, and dependent on, the start of the mitotic cell cycle. A repeated promoter element (CACGA4) (refs 7-9) and two trans-acting activators (SWI4 and SWI6) have been identified, which are responsible for the periodic and start-dependent transcription of HO. To understand further the link between start and HO transcription, the SWI6 gene has been cloned and sequenced. The SWI6 protein is similar to the protein in Schizosaccharomyces pombe that is encoded by cdc10 an essential gene specifically required at the start of the cell cycle. The similarity between the SWI6 and cdc10 products, and their common involvement with 'start', suggest that they may share a common mechanism for sensing or executing this critical control step in the cell cycle. The SWI6 and cdc10 proteins also contain two copies of a repeated motif that occurs at least five times in the cytoplasmic domain of the Notch protein of Drosophila melanogaster.

Amino Acid Sequence↗