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[Comparative temperature measurement by thermoprobes and infrared pyrometry in the rewarming of frozen kidneys using short wave generators].

Mini pigs' kidneys were frozen to -20 degrees C after initial hypothermic perfusion and cryopreparation with dimethyl sulphoxide. They were subsequently thawed out with short-wave radiation. During this procedure continuous temperature measurement is only possible using infra-red pyrometry without physical contact. After completion of short-wave irradiation at ca. 4 degrees C the surface and internal temperatures are measured with thermoelements. In comparison with measurement by thermoprobes, infra-red pyrometry proves to be quite practicable, with certain limitations. If a homogeneous distribution of temperature in the organ is assumed; the internal temperature could be calculated from the surface temperature. Also, continuous observation of temperature changes ist possible.

Animals↗

Tissue cryopreservation and rewarming study on giant cell tumor of bone.

An attempt has been made of cryopreservation of tissue fragments from 6 GCTB surgical specimens. They have been kept in 10% DMSO or 15% glycerol medium and stored in liquid nitrogen for periods up to 180 days. Tumor cellular components survive the process of slow freezing and rapid thawing as cellular growth is successful in subsequent culture. Though certain reversible ultrastructural changes have been detected, no apparent distinction could be found in microscopic morphology, motility, growth behavior and LDH isozyme pattern between cultured cells of the cryopreserved fragments and those of the fresh ones. Electron microscopy shows that DMSO exceeds glycerol in protecting the ultrastructure of the cells. Slow cooling and rapid thawing is recommended because ice-crystals form in the intercellular space, which causes only light damage to the cells.

Bone Neoplasms↗

Rewarming hypothermic postanesthesia patients: a comparison between a water coil warming blanket and a forced-air warming blanket.

The warming effects of a Blanketrol water coil-heated hypothermia blanket and a Bair Hugger forced-warm air warming blanket were compared. Thirty-two patients admitted to the PACU with temperatures 34.4 degrees C (94 degrees F) or lower were assigned to treatment with the Blanketrol (Cincinnati Sub-Zero Products, Cincinnati, OH) or the Bair Hugger (Augustine Medical, Eden Prairie, MN) in alternating fashion, and treatment continued until the patients' temperatures reached 36.1 degrees C (97 degrees F). Every half hour each patient's temperature was measured using a tympanic temperature device and recorded on the data collection sheet. Analysis of the findings showed that the forced-air warming blanket warmed patients to 36.1 degrees C (97 degrees F) or higher significantly faster than the water coil-heated blanket (P < .001).

Adult↗