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Phlebotomus (Adlerius) halepensis vector competence for Leishmania major and Le. tropica.

In Eurasia, phlebotomine sandflies of the subgenus Adlerius (Diptera: Psychodidae) comprise about 20 known species. Some are suspected vectors of visceral leishmaniasis (VL) and at least one species has been implicated as a vector of cutaneous leishmaniasis (CL). We tested Phlebotomus (Adlerius) halepensis Theodor (Jordan strain) for CL vector competence, compared with three standard vectors: Phlebotomus (Phlebotomus) duboscqi N-L. from Senegal, Phlebotomus (Paraphlebotomus) sergenti Parrot from Turkey and the Neotropical Lutzomyia longipalpis (L. & N) (Jacobina strain). Sandfly females were membrane-fed on amastigote suspensions of Leishmania major Y. & S. and Le. tropica (Wright) (Kinetoplastida: Trypanosomatidae) and examined for parasite development 3, 6 and 10 days post-infection. Phlebotomus halepensis showed high susceptibility to both leishmanias, supporting typical suprapylarian parasite development similar to the other vectors. Phlebotomus halepensis infection rates were approximately 90% for Le. major and approximately 80% for Le. tropica, with high parasite densities. Development of infections was relatively fast, colonizing the thoracic midgut by 6 days post-bloodmeal in every case and reaching the stomodeal valve in >80% of flies. In late-stage infections, 10 days post-bloodmeal, nearly all P. halepensis females had cardia and stomodeal valve filled with very high numbers of parasites and some Le. tropica-infected females had promastigotes in the pharynx and proboscis. Host choice experiments in the laboratory showed that P. halepensis females fed readily on rat or rabbit and preferred the human forearm. In view of its vector competence and partial anthropophily, we infer that P. halepensis is a potential vector of cutaneous as well as visceral leishmaniases.

Animals↗

[Presence of Phlebotomus bergeroti, Phlebotomus chabaudi, Phlebotomus chadlii and Sergentomyia christophersi in Morocco].

The authors indicate the presence at Morocco of the four following sandflies: Phlebotomus bergeroti Parrot, 1934, Phlebotomus chabaudi Croset, Abonnenc et Rioux, 1970, Phlebotomus chadlii Rioux, Juminer et Gibily, 1966 and Sergentomyia christophersi (Sinton, 1927). Among them, Phlebotomus bergeroti, an element of eremitic affinity, is found at the north of the Atlas under a sub-humide bioclimat.

Animals↗

[Presence in People's Republic of Congo of Sergentomyia decipiens, Sergentomyia dissimillima, Sergentomyia tauffliebi, Sergentomyia wansoni and Sergentomyia squamipleuris (author's transl)].

The authors indicate the presence in People's Republic of Congo of the five following sandflies: Sergentomyia decipiens Theodor, 1931; Sergentomyia dissimillima Abonnenc, 1972; Sergentomyia tauffliebi Abonnenc and Cornet, 1971; Sergentomyia wansoni Parrot, 1938 and Sergentomyia squamipleuris Newstead, 1912. They give the check-list of sandflies of Congo on the 31st may 1976 and a map of their geographical distribution.

Animals↗

Chinese phlebotomine sandflies of subgenus Adlerius nitzulescu, 1931 (Diptera: Psychodidae) and the identity of Phlebotomus sichuanensis Leng & Yin, 1983. Part I--Taxonomical study and geographical distribution.

Four species of Adlerius phlebotomine sandflies have been recorded in China, namely: P. chinensis Newstead, 1916 (Pc), P. fengi Leng & Zhang, 1994; P. longiductus Parrot, 1928 and P. sichuanensis Leng & Yin, 1983 (Ps). Adlerius phlebotomies are the main vectors of visceral leishmaniasis (VL) in China; three of them are acknowledged as VL vectors and P. fengi is considered a potential VL vector for southwestern mountainous region. Different opinion has been raised to the validity of identity of Ps by some investigators from Shanghai and Shanxi who consider Ps to be a large type of Pc instead of an isolate species. The center of controversy is whether Ps is an isolate taxon or a large type of Pc. The present authors have carried out a series of comparative studies for these two flies on: 1 quantitative and qualitative morphological characters of four Chinese Adlerius phlebotomies; and 2. differences in geographical distribution. All specimens of Pc and Ps used in the present study are collected where their holotypes-paratypes were produced--West Mountain, West Suburb, Beijing and Lixian County, Sichuan Province. The results have forcefully proved that Ps is an isolate species instead of a so-called large type Pc according to the concept of species. The clarification of their taxonomical identities is meaningful because both of them are VL vectors in different epidemic areas in China; especially Ps is an important VL vector in high mountainous regions of southwestern China and some extend to the Loess Plateau of northwestern China, where VL still exists and it is also the first Phlebotomine sandfly discovered in Tibet, the locality being near Assam in India (Leng et al. 1990).

Animals↗

Recovery of hearing and vocal behavior after hair-cell regeneration.

Postmitotic hair-cell regeneration in the inner ear of birds provides an opportunity to study the effect of renewed auditory input on auditory perception, vocal production, and vocal learning in a vertebrate. We used behavioral conditioning to test both perception and vocal production in a small Australian parrot, the budgerigar. Results show that both auditory perception and vocal production are disrupted when hair cells are damaged or lost but that these behaviors return to near normal over time. Precision in vocal production completely recovers well before recovery of full auditory function. These results may have particular relevance for understanding the relation between hearing loss and human speech production especially where there is consideration of an auditory prosthetic device. The present results show, at least for a bird, that even limited recovery of auditory input soon after deafening can support full recovery of vocal precision.

Animals↗

Functional anatomy of neural pathways contributing to the control of song production in birds.

In birds, as in humans, vocal control involves the intricate coordination of three major groups of muscles, namely, those of the vocal organ, the respiratory apparatus, and the vocal tract, including the jaw and tongue. The neural pathways involved in the control of each of these groups of muscles are described for songbirds and compared with those in non-oscine birds and mammals. The pathway in songbirds that controls the syrinx, the bird's vocal organ, originates in the telencephalon and projects via the occipito-mesencephalic tract directly upon vocal motoneurons in the medulla. Activity in this pathway configures the syrinx into phonatory positions for the production of species typical vocalizations. Another component of this pathway mediates control of respiration during vocalization, since it projects upon both expiratory and inspiratory groups of premotor neurons in the ventrolateral medulla, as well as upon several other nuclei en route. This pathway appears to be primarily involved with the control of the temporal pattern of song, but is also importantly involved in the control of vocal intensity, mediated via air sac pressure. There are extensive interconnections between the vocal and respiratory pathways, especially at brainstem levels, and it may be these that ensure the necessary temporal coordination of syringeal and respiratory activity. The pathway mediating control of the jaw appears to be different from those mediating control of the syrinx and respiratory muscles. It originates in a different part of the telencephalon and projects upon premotor neurons in the medulla that, on preliminary analysis, appear to be separate from those projecting upon the syringeal motor nucleus. The separateness of this pathway may reflect the imperfect correlation of jaw movements with the dynamic and acoustic features of song. The brainstem pathways mediating control of vocalization and respiration in songbirds have distinct similarities to those in non-oscine birds and in mammals such as cats and monkeys. However, songbirds and parrots, like humans, but unlike other non-songbirds, have developed a special telencephalic vocal control system for the production of learned vocalizations.

Animals↗

The percentage of feline, canine, avian and exotic animal consultations in veterinary practice in The Netherlands in 1994 and suggested consequences for the veterinary curriculum and residency programs.

The percentage of feline, canine, avian and exotic animal consultations in 1994 was established by means of a survey among veterinary practitioners in the Netherlands. The results from the response to a questionnaire which was sent to companion animal practitioners were compared with those of an at random telephone survey and tested for reliability. Results showed that the cat tended to be the most frequently presented species in companion animal practice in the Netherlands in 1994 (46%), followed by dogs (44%) and birds and exotic animals (10%). In mixed practices these percentages were 40%, 51%, and 9% respectively. From the 'avian and exotic animal' group the following species or orders were the most important: rabbits (32%), rodents (26%), parrots (12%), pigeons (9%), songbirds (9%), ferrets (4%), others (8%). It is concluded that the veterinary curriculum should be adapted to prepare veterinary students to deal with this greater variety in pet animals species and related problems. The need to include exotic animal medicine in resident training programmes is emphasized.

Animals↗

Differences in the biotransformation of 2,4,6-trinitrotoluene (TNT) between wild and axenically grown isolates of Myriophyllum aquaticum.

The aim of this study was to demonstrate the potential for aquatic plants and their associated microbes to bioremediate wetland sites contaminated with 2,4,6-trinitrotoluene (TNT). The transformation of TNT was studied using both wild and axenically grown isolates of Myriophyllum aquaticum (parrot feather). Differences in TNT transformation rates and nitroaromatic metabolites were observed between different plants. The wild isolates, containing a consortium of associated microorganisms, transformed TNT into 2-amino-4,6-dinitrotoluene (2-A-DNT) and 4-amino-2,6-dinitrotoluene (4-A-DNT) via 2- and 4-hydroxylamino-dinitrotoluene, which were detected as intermediates. The wild M. aquaticum also converted the metabolites, 2-A-DNT and 4-A-DNT, into low levels of 2,4-diaminotoluene (2,4-DAT). The axenically grown plants, containing no cultureable microorganisms, also transformed TNT into 2-A-DNT and 4-A-DNT, but at a much lower rate than that observed for the wild isolates. Unlike the wild plants, axenically grown M. aquaticum could not transform either 2-A-DNT or 4-A-DNT into 2,4-DAT over the incubation period. The differences in the performance between these plants could indicate that plant-associated microorganisms assisted in the overall transformation of TNT. For each plant, unidentifiable metabolites were observed and the soluble monoamino-derivatives present in the wild and axenic medium accounted for 14 and 7% of the initial TNT concentration, respectively. Thus, the majority of nitroaromatic derivatives remained associated with the plant tissues. Furthermore, only 7 and 3% of the initial TNT concentration were extracted as monoamino-derivatives from the tissues of the wild and axenically grown plants, respectively.

Aniline Compounds↗

Phytoremediation potential of Myriophyllum aquaticum and Pistia stratiotes to modify antibiotic growth promoters, tetracycline, and oxytetracycline, in aqueous wastewater systems.

Antibiotics are frequently used in the United States as feed efficiency promoters and medicines for livestock that is destined for human consumption. These antibiotics are released into the environment through the runoff and wastewater streams from animal feedlots and land applications of manure. The exposure of microorganisms to these antibiotics has reportedly resulted in the development of resistant species of microorganisms, which in turn can lead to human health hazards. Phytoremediation of these antibiotics can be a useful tool for countering this problem. Aquatic plants, Myriophyllum aquaticum (parrot feather) and Pistia stratiotes (water lettuce), were used for studying phytoremediation of tetracycline (TC) and oxytetracycline (OTC) from aqueous media. TC and OTC are two of the most commonly used tetracyclines in veterinary medicine. M. aquaticum and P. stratiotes gave high antibiotic modification rates of both antibiotics. Kinetic analyses dismiss direct enzyme catalysis; the modification rates decreased with increasing OTC concentrations. Sterile, cell-free root exudates (filtered through 0.2 microm membranes) from both species also exhibited comparable antibiotic modification rates. The involvement of root-secreted metabolites in antibiotic modification is suggested. The changes in the UV absorbance spectra of OTC during treatment with the root exudates confirmed the modification.

Anti-Bacterial Agents↗

Role of mutation in the gyrA and parC genes of nalidixic-acid-resistant salmonella serotypes isolated from animals in the United Kingdom.

To answer the question as to whether isolates of salmonella from animals with decreased susceptibility to quinolones possess the same mechanism of resistance as isolates from humans, the polymerase chain reaction (PCR) was used to amplify a 347 base pair fragment equivalent to codons 39-159 covering the quinolone resistance determining region (QRDR) of the gyrA gene of Salmonella typhimurium NCTC 74, and 196 veterinary isolates of 26 serotypes of salmonella (109 from turkeys, 29 from chickens, the remainder from cattle, parrots, pigs, dogs, a pigeon, an exotic bird, a duck, a partridge, a sheep, a horse and environmental samples including litter, a nest and water). All PCR products were electrophoresed for single-stranded conformational polymorphism (SSCP). The DNA sequence of all eight novel SSCP patterns was determined. Nucleotide substitutions were identified in the gyrA gene of all except two isolates; all substitutions conferred a substitution at serine 83 or aspartate 87, as has previously been shown for isolates from humans. No mutations conferring an amino acid substitution in parC (the gene encoding the secondary target for quinolones) were revealed in the 48 isolates requiring 0.5-1 mg/L ciprofloxacin for inhibition, or the isolates for which no mutation in gyrA was revealed. The accumulation of enrofloxacin and ciprofloxacin was determined for 30 representative isolates (of which five were multiply drug resistant). Five isolates accumulated one-quarter to one-half the concentration achieved by the corresponding wild-type serotype. For two of these five isolates no mutation in gyrA was revealed, and one lacked OmpF.

Animals↗

Differentiation of vector species of phlebotominae (Diptera: Psychodidae) in Kenya by chorionic sculpturing of their eggs.

Chorionic sculpturing on eggs of Phlebotomus pedifer Lewis, Mutinga, and Ashford; the closely related Phlebotomus aculeatus Lewis, Minter, and Ashford (= P. elegonensis Ngoka, Madel, and Mutinga); and Phlebotomus martini Parrot was examined and compared by scanning electron microscopy. The eggs of P. pedifer had a general pattern of longitudinal ridges; those of P. aculeatus and P. martini exhibited intraspecific differences. Chorionic patterns of eggs were not reliable to differentiate these species. It is suggested that other methods of differentiation should be used on these species.

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Effects of permethrin-treated screens on phlebotomine sand flies, with reference to Phlebotomus martini (Diptera: Psychodidae).

Effects of permethrin-treated screens on the sand fly, Phlebotomus martini Parrot, a vector of visceral leishmaniasis in Kenya, were evaluated after the eighth treatment. Screens treated with 0.50 g/m2 of permethrin 20% emulsifiable concentrate were fitted inside houses and re-treated every 6 mo from August 1989 to December 1993. Before application of the treated screens, a geometric mean of 0.83 P. martini were collected per trap night inside houses, whereas after the eighth treatment only 0.05 sand flies were caught. P. martini abundance was reduced by 88.8%. Analysis of the effects of treated screens on the total sand fly population (all species) indicated that 1.79 sand flies were collected per night per house before deployment of treated screens, whereas 0.16 flies were caught after the eighth treatment. The percentage of sand fly reduction inside treated houses increased with successive treatment of the screens, and a reduction of 81.4% was observed after the eighth treatment. Permethrin-treated screens did not reduce the sand fly engorgement rate inside houses.

Animals↗

Redescription of Alcedinectes alcyon (Acari:Hypoderatidae) from the belted kingfisher (Aves:Coraciiformes; Alcedinidae).

Alcedinectes alcyon (Boyd) is redescribed based on specimens collected from its belted kingfisher, Megaceryle alcyon (L.), type host in Florida. This species differs from A. cerylei Fain by the chaetotaxy of tarsus III. A. alcyon has a long stout apical seta that tapers to a curved tip resembling that seen on tarsus III of the genus Tytodectes (Ispidectes) Fain. The equivalent seta in A. cerylei is a stout spine. There are other minor differences between these species in the pattern of idiosomal sclerotization and leg chaetotaxy. The chaetotaxy and solenidiotaxy of legs I and II in Alcedinectes closely resemble that of Amazonectes psittaci Fain & Vercammen-Grandjean from parrots (Psittaciformes), whereas the morphology and chaetotaxy of the idiosoma resemble that of Tytonectes (Ispidectes) spp. which also occurs in kingfishers (Coraciiformes). The long stout seta that tapers to a curved tip on tarsus III in A. alcyon also links the genus Alcedinectes with Tytodectes (Ispidectes).

Animals↗

Morphometric separation of females of Phlebotomus (Phlebotomus) duboscqi and P. (P.) bergeroti (Diptera:Psychodidae).

Morphometric evaluation of 27 characters of allopatric females of the closely related species, Phlebotomus duboscqi Neveu-Lemaire and P. bergeroti Parrot, was made in Ethiopia with the aim of finding reliable means to distinguish sympatric specimens. By applying stepwise discriminant analysis, 100% correct classification was obtained using 8 characters. However, 2 of these characters, C3 and C4 (respective distances from sockets of the longest ascoids on segment 3 and 4 to distal margin of the segment) with the highest discriminant loadings, separated these 2 species with a success rate of 98%. It is recommended that these 2 characters be used in the routine identification of these species.

Animals↗

Entomological investigations in Chichaoua: an emerging epidemic focus of cutaneous leishmaniasis in Morocco.

Cutaneous leishmaniasis due to Leishmania tropica Wright seems to be an emerging disease in Chichaoua, a province located in southwestern Morocco. In this study, sand flies (Diptera: Psychodidae) were collected from 12 stations. Sticky traps were placed in domestic, peridomestic, and sylvatic sites. In total, 3,787 specimens consisting of 10 species (seven Phlebotomus and three Sergentomiya) were identified. Phlebotomus perniciosus Newstead, the predominant species, was abundant, especially in mountainous areas. Phlebotomus sergenti Parrot (12%) was found in all studied villages where it was associated with domestic and peridomestic habitats. On the basis of its abundance, distribution, and notable anthropophily, P. sergenti, a proven vector of L. tropica elsewhere, is considered the cutaneous leishmaniasis vector in this emerging focus.

Animals↗

Mitochondrial genomes and avian phylogeny: complex characters and resolvability without explosive radiations.

We improve the taxon sampling for avian phylogeny by analyzing 7 new mitochondrial genomes (a toucan, woodpecker, osprey, forest falcon, American kestrel, heron, and a pelican). This improves inference of the avian tree, and it supports 3 major conclusions. The first is that some birds (including a parrot, a toucan, and an osprey) exhibit a complete duplication of the control region (CR) meaning that there are at least 4 distinct gene orders within birds. However, it appears that there are regions of continued gene conversion between the duplicate CRs, resulting in duplications that can be stable for long evolutionary periods. Because of this stable duplicated state, gene order can eventually either revert to the original order or change to the new gene order. The existence of this stable duplicate state explains how an apparently unlikely event (finding the same novel gene order) can arise multiple times. Although rare genomic changes have theoretical advantages for tree reconstruction, they can be compromised if these apparently rare events have a stable intermediate state. Secondly, the toucan and woodpecker improve the resolution of the 6-way split within Neoaves that has been called an "explosive radiation." An explosive radiation implies that normal microevolutionary events are insufficient to explain the observed macroevolution. By showing the avian tree is, in principle, resolvable, we demonstrate that the radiation of birds is amenable to standard evolutionary analysis. Thirdly, and as expected from theory, additional taxa breaking up long branches stabilize the position of some problematic taxa (like the falcon). In addition, we report that within the birds of prey and allies, we did not find evidence pairing New World vultures with storks or accipitrids (hawks, eagles, and osprey) with Falconids.

Animals↗

Identification of a 16--17-day lethal factor in a chondrodystrophic stock of turkeys.

Chondrodystrophy in turkeys is a lethal condition produced by an autosomal allele, ch. Mutants are characterized by very short and thick legs, shortened wings, globular head with parrot beak, and a protruding abdomen. Classification of ch/ch embryos may be accurately made at 12 days incubation, prior to which limited appendicular development precludes distinction from normal or heterozygous birds. Death caused by the mutant allele occurs between 24 and 28 days; some embryos are alive but unpipped at time of hatch. Analysis of the pattern of embryonic mortality for the stock carrying the ch allele revealed at 16--17-day lethal factor that appears to be independent of chondrodystrophy. Of 52 females mated to Ch/ch males, 41 were determined to be Ch/ch; 32 of these heterozygous females exhibited normal embryonic mortality patterns. Nine hens, in addition to being Ch/ch, displayed a significant increase in embryo death between 15 and 18 days, peaking sharply at 17 days. Of the 11 females determined to be Ch/Ch, five showed the same 15 to 18 day pattern of mortality observed for the nine Ch/ch females.

Animals↗

A new chondrodystrophy mutation in Japanese quail (Coturnix japonica).

A second form of hereditary chondrodystrophy (ch-2) has been discovered in a selected line of Japanese quail, Coturnix japonica. This form of chondrodystrophy is autosomal and recessive, characterized by an overall shortening and bending of the long bones of the wings and legs, slight dwarfing of the trunk, bulging of the eyes, flattening of the head, and a parrot beak. The shortened long bones vary in regard to the amount of bending from nearly straight to bends of up to 90 degrees in the midshaft region. In severe cases, the bend is evident as a protuberance of the skin. Affected embryos usually survive the 18-day incubation period. Several have hatched, but most survived no longer than 4 days after hatching. Only one female has survived long enough to lay eggs. Testcrosses indicated that this mutation is not allelic to micromelia.

Animals↗