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At least 595 records · Page 33Linked to original sources
PubMed1979

Convenient method for the gas chromatographic analysis of hexosamines in the presence of neutral monosaccharides and uronic acids.

A convenient gas chromatographic method has been devised for the analysis of hexosamines in the presence of neutral and acidic sugars, which involves sequential derivatization reactions of nitrous acid deamination, mercaptalation, and trimethylsilylation. This method allows rapid, simultaneous determination of 0.1-1 micromole samples of hexosamines with coefficients of variation of less than 3%.

Adult↗
PubMed1989

Evaluation of the liquid chromatographic separation of monosaccharides, disaccharides, trisaccharides, tetrasaccharides, deoxysaccharides and sugar alcohols with stable cyclodextrin bonded phase columns.

Both alpha- and beta-cyclodextrin (CD) bonded phase columns were evaluated for their ability to separate carbohydrates and related molecules. Chromatographic data on approximately 50 solutes are reported. Mobile phases consisting of acetonitrile-water or acetone-water produce the best separations. Separations could be run in isocratic or gradient modes. The alpha-CD column produced slightly more efficient separations than the beta-CD column. Both cyclodextrin columns seemed to be more efficient and selective than alkylamine and ion-exchange columns. The stability and reproducibility of the CD columns were excellent and showed little deterioration after several thousand injections. The retention of saccharides on CD bonded phases seems to be related to the number of available hydroxy groups per solute and to the size of the solute. Analysis times and detection sensitivity are discussed.

Chromatography, Liquid↗
PubMed2002

Simultaneous analysis of monosaccharides and oligosaccharides by high-performance liquid chromatography with postcolumn fluorescence derivatization.

To develop a fluorimetric HPLC technique for the simultaneous microanalysis of reducing mono- and oligosaccharides, the technique of linear gradient elution was introduced into the postcolumn fluorimetric detemination system of reducing saccharides with benzamidine. Fluorescence measurement was performed at 288 nm for excitation and 470 nm for emission and an optimization study for this postcolumn fluorescence derivatization carried out. Under optimum conditions, the detection limits of D-glucose and maltohexaose were 1.78 and 2.59 pmol, respectively. The present method was successfully applied to saccharide analysis and should prove useful for automated simultaneous microanalysis of reducing mono- and oligosaccharides in foods.

Chromatography, High Pressure Liquid↗
PubMed2003

Non-aqueous capillary electrophoresis of the positional isomers of a sulfated monosaccharide.

A non-aqueous capillary electrophoresis (NACE) method coupled to indirect absorbance detection has been developed for the separation of the three positional isomers of monosulfated fucose. The optimized electrolyte was composed of 12 mM ethanolamine, 2 mM trimesic acid buffer in a methanol-ethanol (1:1, v/v) mixture. As the retained electrolyte entails no separating agent other than the pH buffer, the NACE separation of the positional isomers has been ascribed mainly to selective ion-pairing with the electrolyte counter-ion and the possibility of a selective solvation effect in the alcohol mixture. In the absence of pure isomeric standards, peak identification was completed by MS and NMR spectroscopy and selective enzymatic desulfation. This method should be of interest for the structure elucidation of monosulfated fucose-based polysaccharides and for the screening of sulfoesterase of unknown activity.

Electrophoresis, Capillary↗
PubMed1998

Similarity of monosaccharide, oligosaccharide and polysaccharide structures in gum exudate of Anacardium occidentale.

The gum exudate from the Brazilian cashew-nut tree (Anacardium occidentale) contained traces of the reducing sugars, rhamnose (0.005%), arabinose (0.03%), mannose (0.007%), galactose (0.03%), glucose (0.02%), beta-D-Galp-(1-->6)-alpha beta-D-Gal (0.05%), alpha-L-Rhap-(1-->4)-alpha beta-D-GlcA (0.008%) and alpha-L-Rhap-(1-->4)-beta-D-GlcpA-(1-->6)-beta-D-Galp-(1-->6 )-alpha beta-D-Gal (0.008%). Rhamnose, arabinose, glucose and the three oligosaccharides are components of the side-chains of the gum polysaccharide, which has a main chain of (1-->3)-linked beta-D-Galp units. The structure of this polysaccharide was determined and found to differ from that previously reported for the gum of a tree growing in India, lacking units of 4-O-methylglucuronic acid. Other new side-chain structures were characterized, particularly -alpha-D-Galp-(1-->6)-D-Galp- and alpha-L-Araf-(1-->6)-D-Galp-).

Carbohydrate Sequence↗