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At least 595 records · Page 33Linked to original sources
PubMed2006

Ecotoxicological evaluation of the wastewater treatment process of the sewage treatment plant of Thessaloniki, Greece.

The LUMISTox toxicity test was employed to assess the removal of municipal wastewater toxicity during the biological treatment with activated sludge in the wastewater treatment plant of Thessaloniki, Greece. Possible associations of toxicity data with chemical parameters of organic pollution of wastewaters, namely BOD(5), COD, DOC, SS and persistent organic pollutants (POPs) were also investigated. Toxicity and chemical parameters were concurrently measured at three sampling points of the treatment plant, the entrance of the unit (raw wastewater, RW), the effluent of the secondary sedimentation tank (SSE), and the final sewage sludge (FS). Substantial reduction of toxicity was observed from RW to SSE (mean+/-S.D. of bioluminescence inhibition 36+/-9.4% and 13+/-4.0%, respectively) indicating removal of toxicants during primary and secondary clarification, also suggesting that a large part of the toxicity measured is attributed to the biodegradable fraction of the organic content of wastewater. Significant positive correlations were observed between % inhibition values and wastewater parameters (BOD, COD, SS). In sludge, correlations were in general poor. Negative strong correlation was observed between EC(20/15) and TOC suggesting that the organic content of sludge contributes to the toxicity measured. Toxicity was positively correlated with the concentrations of certain POPs in RW, while weaker negative correlations were observed in SSE. Correlations in sewage sludge were less significant. It was concluded that toxicity testing is a useful tool supplementing chemical analyses in the evaluation of the potential hazard from effluent discharges and disposal of waste sludge.

Aliivibrio fischeri↗
PubMed2004

The deciduous lower dentition of Ouranopithecus macedoniensis (Primates, Hominoidea) from the late Miocene deposits of Macedonia, Greece.

Two mandibular fragments with associated milk teeth assigned to the late Miocene hominoid primate Ouranopithecus macedoniensis are analyzed. The fossils, which belong to a single individual, were found in the Vallesian locality of "Ravin de la Pluie" of the Axios Valley (Macedonia, Greece). The material is described here and compared with extant and extinct hominoids, allowing assessment of the evolutionary trends in the deciduous lower dentition within the Hominoidea. Hylobatids represent the more primitive pattern. Gorilla is slightly more derived than hylobatids, but less derived than Pongo and Pan, the latter being the most derived. With relatively smaller deciduous canines and more molarized deciduous premolars, Ouranopithecus is more derived than both Pan and Gorilla. Among the fossil hominoids, Proconsul, representing the primitive condition, has a very simple dp(3)and a dp(4)that has a trigonid that is taller than the talonid and which lacks a hypoconulid. Griphopithecus is more derived than Proconsul in having a dp(4) with a lower trigonid, a hypoconulid, and a less oblique cristid obliqua. Australopithecus and Paranthropus possess a similar morphology to that of Homo, while Ardipithecus appears to be more primitive than the latter genera. Ouranopithecus has a more derived lower milk dentition than Proconsul and Griphopithecus, but less derived than Australopithecus and Paranthropus. The comparison of the lower milk dentition of Ouranopithecus confirms our previous conclusions suggesting that this fossil hominoid shares derived characters with Australopithecus and Homo.

Animals↗
PubMed2004

Molecular characterization of Mycobacterium tuberculosis isolates presenting various drug susceptibility from Greece using three DNA typing methods.

OBJECTIVES: The purpose of the present study was the molecular characterization of Mycobacterium tuberculosis clinical isolates using three DNA typing methods. METHODS: One hundred nineteen independent (77 susceptible to all antituberculous drugs, 17 rifampin-resistant and 25 isoniazid-resistant), and nine related Mycobacterium tuberculosis isolates obtained over a 3-years period (1997-1999) from Greece were typed with restriction fragment length polymorphism (RFLP), using the non-radioactive IS6110 probe (IS6110-RFLP), and two PCR-based molecular methods: random amplification of polymorphic domains (RAPD) using four different primers and double repetitive element-PCR (DRE-PCR). RESULTS: IS6110-RFLP and RAPD-PCR using IRIS primer were proved to be the most discriminatory methods, while DRE-PCR gave satisfactory results and RAPD-PCR methods using the other three primers (A1245, B1245 and Leg2) were not so effective. The related strains, isolated from affected members of four families, gave similar PCR and RFLP patterns, while the independent strains presented a high degree of polymorphism. In terms of cost effectiveness and technical simplicity, the PCR-based methods were found to be superior. CONCLUSIONS: So, they may serve as screening methods to classify a large number of isolates into clusters for further subtyping and recognition of well-defined genotype families.

Adult↗
PubMed2004

An association study of the tumor necrosis factor alpha C-850T polymorphism and childhood acute lymphoblastic leukemia in a population from northern Greece.

Excessive production of tumor necrosis factor alpha (TNFalpha) may influence the risk and/or progression of hematologic malignancies and has been associated with febrile episodes at diagnosis. We have examined the putative association of the C-850T polymorphism of the human TNFalpha gene with childhood acute lymphoblastic leukemia (ALL) and with clinical and laboratory findings at diagnosis, in 58 childhood ALL patients from northern Greece. No statistically significant associations have emerged between this polymorphism and either the risk for childhood ALL or presence of fever, anemia, leukocytosis and leukopenia at diagnosis, in this study.

Adolescent↗
PubMed2004

DDTs and other chlorinated organic pesticides and polychlorinated biphenyls pollution in the surface sediments of Keratsini harbour, Saronikos gulf, Greece.

Sediment samples were collected from Keratsini harbour, Saronikos gulf, Greece and were analysed for chlorinated organic pesticides (DDTs, HCB, Lindane) and polychlorinated biphenyls (PCBs). High total DDTs values were detected in all the sediments samples ranging from 9.1 to 75.6 mug/g, dry weight. PCBs concentrations range from 47.8 to 351.8 ng/g. The results and especially the high concentrations of DDTs reflect the influence of the industrial and urban wastes in the pollution for the Keratsini harbour environment.

DDT↗
PubMed2006

Genotyping of pathogenic Acanthamoebae isolated from clinical samples in Greece--report of a clinical isolate presenting T5 genotype.

Amoebae belonging to the genus Acanthamoeba are potentially pathogenic to humans, causing mainly amoebic keratitis. Pathogenic ability of the 15 known Acanthamoeba genotypes is under investigation. We report that four out of five cases of amoebic keratitis studied in Greece, present T4 sequence type, while the remaining one presents T5 sequence type (Acanthamoeba lenticulata), which is the second most frequent genotype found among environmental samples. Thus, it is confirmed, for the first time to our knowledge, that A. lenticulata can cause keratitis. However the reason that it is under represented in clinical samples compared to environmental ones is unknown.

Acanthamoeba↗
PubMed2006

Ecology of testate amoebae from mires in the Central Rhodope Mountains, Greece and development of a transfer function for palaeohydrological reconstruction.

Testate amoebae are useful environmental indicators in ecological and palaeoecological studies from peatlands. Previous quantitative studies have focused on the peatlands of Northern and Central Europe, North America, and New Zealand and have considered a relatively restricted variety of peatland types, mostly ombrotrophic or Sphagnum-dominated while more minerotrophic fens have been less studied. Here we present the first quantitative ecological study of testate amoebae from four small mesotrophic fens (pH 5.5-8.1) in the Elatia Forest, northern Macedonia province, Greece. Relationships with the environmental data were investigated using redundancy analysis and mantel tests. Transfer function models were derived using a variety of techniques. Results demonstrate that as for Sphagnum-dominated mires hydrology is the most important control on amoebae community composition. Transfer function models should enable water tables to be predicted within 2.5 cm, when data selection is used this is reduced to less than 2 cm. pH is also an important environmental control on testate amoebae communities, a transfer function model enables pH prediction within 0.4 pH units. The hydrological transfer function is the best performing such model yet produced in terms of prediction error. This study provides new data on the ecology of testate amoebae in fens, and the transfer function models should allow quantitative palaeohydrological reconstruction.

Amoeba↗
PubMed2004

International EMS systems: Greece.

Emergency Medical Services (EMS) in Greece have been created to offer first aid, primary medical treatment if required, stabilisation and safe and rapid transfer to the closest appropriate hospital. To achieve these objectives, organised systems have been established with trained personnel and physicians, suitable equipment, communication centres and protocols. We present the Greek EMS system and explain its history, structure, training, cooperation with Emergency Departments (ED) and an outlook on the future.

Emergency Medical Services↗
PubMed2004

The potential of pesticides to contaminate the groundwater resources of the Axios river basin in Macedonia, Northern Greece. Part I. Monitoring study in the north part of the basin.

The Axios river basin is an area of high agricultural importance for Macedonia, Northern Greece. High priced crops such as cotton, corn, sugarbeets, vegetables and rice are rotated with cereals and alfalfa and thus a great variety of pesticides are used annually for the control of pests and diseases. Moreover, the groundwater resources of the specific area constitute the main source of potable water for the surrounding cities and villages. Therefore, a study was initiated to investigate the probable risks of groundwater contamination with pesticides. The leaching of pesticides from agricultural soils was monitored in the phreatic horizon as well as in soil water samples collected with suction lysimeters. In parallel studies, the presence of pesticides in the irrigation water and the water of Axios river was also monitored. Pesticides like carbofuran, atrazine, alachlor and prometryne, which were regularly applied in the northern part of the basin during 1992-1994 were detected at concentrations occasionally exceeding 1 microg/l in soil water from the deeper soil layers and in the phreatic horizon. Residues of pesticides others than the ones applied in the studied area like propanil, terbufos and paraoxon-methyl were also detected at the deeper soil layers and in the phreatic horizon. The latter pesticides were also detected at significant concentrations in the irrigation water which mainly consisted of riverine water. The lack of an independent soil draining system and the use of riverine water for irrigation purposes resulted in the redistribution of residual amounts of these pesticides in the phreatic horizon beneath the agricultural area.

Agriculture↗
PubMed2004

Effects of nitrogen deposition on nitrogen cycling in an Aleppo pine stand in Athens, Greece.

High levels of nitrogen deposition measured in throughfall fluxes in 2002, reaching 38 kg ha(-1) year(-1), affected the biogeochemical cycle of N in an Aleppo pine (Pinus halepensis) stand situated in Athens, the capital of Greece. The N fluxes in bulk deposition were far lower than those in throughfall indicating high levels of dry deposition in the city of Athens. The amounts of N in litterfall and those stored in the forest floor and mineral soil were not high, while the mean residence time of N in the forest floor, derived from fluxes equations, decreased considerably due to the high levels of N fluxes in throughfall deposition. Nitrogen concentration in needle tissues was high and this resulted in a high ratio of N/Zn. Zinc availability was low due to the alkaline nature of soil in the study area. Fast N cycling may affect the living status of Aleppo pine, which is an oligotrophic plant species capable of occupying poor soil sites. Nitrogen eutrophication may adversely affect forests grown on calcareous soils by developing nutrition imbalances between N and micronutrients, the solubility of which depends on soil pH values.

Environmental Monitoring↗
PubMed2005

Indoor and outdoor personal exposure to benzene in Athens, Greece.

OBJECTIVE: To evaluate the exposure of urban inhabitants to atmospheric benzene in Athens, Greece. METHODS: Fifty non-smoker volunteers from selected occupational groups and their homes were monitored by passive air samplers for six 5-day periods during a year. An activity diary was completed during each sampling period and relevant data were collected by a questionnaire at the beginning of the study. Additional data on urban levels on benzene were also available. RESULTS: Average benzene home and personal levels in six monitoring campaigns varied between 6.0-13.4 and 13.1-24.6 microg/m(3), respectively. Urban levels varied between 15.4 and 27.9 microg/m(3) with an annual mean of 20.4 microg/m(3). Wind speed seems to determine largely home levels and personal exposure. Proximity to busy road holds also an important influence on indoor benzene levels. Adjusted for seasonal or climate variation, other significant prognostic factors of personal exposure were home levels, total time spent outdoors and transportation mean. Time spent outdoors explains the strong relationship between occupation and personal levels of exposure. Wind had similar effect in clearing indoor and urban pollution in Athens; lessen personal exposure and home levels about 2-2.5 microg/m(3) per 1 m/s increase in speed. CONCLUSIONS: Factors related to climate (use of non-absorbent materials for wall and floor covering and frequent ventilation) might be one explanation for homes' high clearing rate. Our exposure pattern, which suggests that outdoors work give the greater contribution to benzene exposure of Athens citizens, is uncommon in northern towns of Europe. Policy makers have to take in account these differences in establishing guidelines for ambient benzene exposure.

Air Pollutants↗
PubMed2006

Detection of the marine toxin okadaic acid in mussels during a diarrhetic shellfish poisoning (DSP) episode in Thermaikos Gulf, Greece, using biological, chemical and immunological methods.

An approach involving chemical and biological techniques was taken for the detection and quantification of the marine toxin okadaic acid (OA) in mussels from Thermaikos Gulf, Greece, during a 4-month DSP episode that occurred in 2002. Samples were analyzed using the mouse bioassay, high performance liquid chromatography (HPLC) with fluorimetric detection and an enzyme-linked immunosorbent assay (ELISA). Okadaic acid was quantifiable at three different sites of Thermaikos Gulf reaching a maximum concentration of 36 microg/g hepatopancreas. High correlation was revealed between results derived from HPLC and ELISA (R2=0.998), while 91% consistency between HPLC and the mouse bioassay results was observed.

Animals↗
PubMed2006

Lead concentrations and isotope ratios in street dust in major cities in Greece in relation to the use of lead in petrol.

Until recently, the most important source of environmental lead pollution in cities was thought to come from the combustion of leaded petrol. A simple way to monitor the extent of this phenomenon, used in a number of studies in the past, has been to measure lead levels in street dust. Nowadays, it would be expected that lead concentrations in urban dust would have decreased from earlier values, following the progressive reduction of lead in petrol over the past few years, and this hypothesis has recently been confirmed in Manchester, UK. The object of the present work is to determine levels of lead pollution in cities in Greece on 1997 and, if possible, to discover whether similar reductions in lead concentrations have occurred there also. Surveys have been conducted in Thessaloniki, Athens and Piraeus. Samples of roadside dust were collected from streets (categorised by traffic density), national gardens and school playgrounds, and lead was extracted by digestion with concentrated nitric acid. Lead concentrations were determined by graphite furnace atomic absorption spectrometry and lead isotope ratios measured by inductively coupled plasma mass spectrometry. Results for Thessaloniki showed that mean lead concentrations in all categories of location are similar to present levels in Manchester. Further, lead concentrations in dust in the busiest streets in Thessaloniki have fallen by about 55% since a previous study 17 years ago. In Athens and Piraeus, the lead levels in street dust are much higher and significant differences were observed between the various types of street. In particular, it was observed that lead levels in school playgrounds in these two cities were much higher than in similar locations in Thessaloniki and Manchester, with a possible hazard to children. Isotope ratio measurements showed that Thessaloniki's lead is isotopically distinct from that found in Athens and Piraeus, which presumably reflects differences in sources of supply.

Cities↗
PubMed2005

Pretransplantation prevalence of human herpes virus 8 antibodies in kidney donors and recipients in Athens, Greece.

AIM: The aim of this study was to estimate the prevalence of anti-human herpes virus 8 (HHV8) antibodies in a cohort of renal donors and recipients in Athens, Greece. HHV8, the etiological agent of posttransplantation Kaposi's sarcoma, causes significant morbidity and mortality. METHODS: Serum samples from 97 subjects (49 donors and 48 recipients) were tested with an enzyme-linked immunosorbent assay (ELISA) prior to renal transplantation. RESULTS: Only 2 subjects (both transplant recipients) were found to be anti-HHV8-positive. Both subjects were of Albanian origin. CONCLUSION: Infection with HHV8 appears to be limited in the Greek population. However, in light of significant long-term morbidity with which HHV8 is related in immunocompromized patients, studies on the general population are needed to estimate the prevalence of HHV8 infection in the country and devise clear guidelines for pretransplantation screening and posttransplantation follow-up.

Albania↗
PubMed2005

A quality assurance program in dental radiographic units in western Greece.

OBJECTIVE: This survey reports on successful efforts to establish a quality assurance (QA) program for 50 intraoral x-ray units in the public and private sectors in the region of Achaia, Greece. It was conducted in 2 phases, in 1996 and in 2003, including on-site inspections, QA tests, and standard questionnaires. The aim of the study was to assess equipment conditions, knowledge, and adoption of radiographic QA guidelines by general dentists and, more importantly, the impact of the recommendations and training provided. STUDY DESIGN: The tested parameters focused on radiation protection, equipment maintenance, film speed used, film processing conditions, and radiologic characteristics such as voltage, radiation leakage, type of collimation, source-to-skin distance, timer accuracy, and entrance dose. RESULTS: The data gathered in 1996 demonstrate minimal compliance with equipment function requirements and radiation safety measures. 1 The comparative evaluation of all the parameters gathered from the 2 surveys, indicated that in 2003 the vast majority of the dentists followed the recommendations given in 1996. Only 2 dentists persisted in neglecting the guidelines.

Filtration↗
PubMed2006

Comparative evaluation of positive tests to Mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west Greece using molecular techniques, serology, and culture.

Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis, which affects mainly ruminants although there is a growing concern about its possible implication in Crohn's disease in humans especially in connection with environmental spread and risks to the food chain. Retail cheese may represent a significant source of human exposure to MAP and the aim of this study was to assess MAP status in clinically healthy sheep and goats in Greece, comparing techniques routinely used in the positive diagnosis of the disease. From a total of 30 flocks, 632 sheep and goats had faecal, serum, and whole-blood samples examined by culture, complement fixation test (CFT), and polymerase chain reaction (PCR) targeted at IS900, IS1245, and IS6110. PCR produced positive results in 21% of the animals tested, with 5.6%, 3.9%, and 11.5% being identified as MAP, Mycobacterium avium subsp. avium, and Mycobacterium tuberculosis complex, respectively. CFT produced positive and suspicious results in 4.4% and 14.4% of the cases. Faecal cultures were negative in all but a single case that was identified as restriction fragment length polymorphism (RFLP)-type BC1. Agreement between results obtained by PCR and CFT was poor with isolated cases although an assessment of the MAP positive tests produced similar results for both methods. The findings indicate the need for additional measures of control, although the costs may be substantial if public health protection justifies elimination of MAP from livestock.

Animals↗
PubMed2005

Determination of prevalence and risk factors of infection with Babesia in small ruminants from Greece by polymerase chain reaction amplification.

A total of 124 blood samples were collected from 92 sheep and 32 goats from 21 randomly selected herds located in two regions of Greece. Data on the characteristics of the animals (species, gender, age, tick burden, presence of haemoglobinuria, prior treatment for babesiosis) and the herd (location, size, species of animals, dogs associated with the herds, tick burden of dogs associated with the herds) were collected through questionnaires. Nineteen animals (15%) produced the DNA fragment specific for Babesia of which 16 were sheep and three were goats. Nucleotide sequence of PCR products revealed 100% homology with Babesia ovis 18S rRNA gene. Nine farms (43%) were found positive for B. ovis. The percentage of positive animals in each farm varied between 10 and 61%. The relative risk of the presence of ticks in sheep and goats (p<0.01) and farm dogs (p<0.01) for PCR-positive results for B. ovis in sheep and goats was found 6.63 and 4.14, respectively.

Animals↗
PubMed2004

Persistent organic pollutants (POPs) in the sewage treatment plant of Thessaloniki, northern Greece: occurrence and removal.

The occurrence and the removal of persistent organic pollutants (POPs) during the conventional activated sludge treatment process were investigated in the wastewater treatment plant of the city of Thessaloniki, northern Greece. POPs of interest were seven polychlorinated biphenyls (PCBs) and 19 organochlorine pesticides. Target compounds were determined at six different points across the treatment system. Most abundant compounds in raw wastewater at all treatment stages were PCB-52, PCB-110, PCB-180 and Heptachlor-exo-epoxide. Quintozene occurred frequently but in relatively low concentrations. Hexachlorocyclohexanes, DDT and its metabolites (DDE, DDD) and Aldrin, Dieldrin, Endrin, Isodrin ("Drins") were found at medium or low frequencies and in concentrations close to their detection limits. Removal percentages throughout the whole treatment process ranged from 65% to 91% for individual POP species. Significant linear relationship was observed between removal efficiency and log Kow for PCBs suggesting that compounds with a strong hydrophobic character are principally removed through sorption to sludge particles and transfer to the sludge processing systems. Total PCBs' concentrations in sewage sludge ranged between 185 and 765 ng g(-1) dw being below the EU limit for use of sludge in agriculture.

Environmental Monitoring↗