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Results for “G6PD”

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PubMed1991

[Purification from goat antiserum of immunoglobulins against G6PD from rabbits].

DEAE Affi-Gel Blue (Bio-Rad) provides an efficient and rapid fractionation of human serum proteins by a single chromatographic step. When goat serum is applied to the matrix and chromatography is performed following the procedure utilized for the human serum proteins, the elution pattern changes and the Ig purification is not satisfactory. We achieved a better Ig purification from goat serum by the following improved procedure. We performed first an AS-40 fractionation followed by extensive dialysis in 50 mM Na-citrate pH 5.7. The sample was then loaded onto a P11 column equilibrated in the same buffer. The fraction eluted at Vo contained total IgG and the other serum proteins, except beta-globulins which were eluted with 0.24 M phosphate. Peak 1 concentrated and dialyzed in 20 mM phosphate buffer pH 8 was then applied to a DEAE Affi-Gel Blue column, equilibrated in the same buffer. Two protein peaks were eluted from this column and electrophoretically characterized as: peak 1, containing a pure Ig fraction (70% yield), peak 2 with albumin and other contaminating serum proteins. When goat antiserum is obtained against a specific protein, our technique may be suitably employed to purify polyclonal antibodies for immunoprecipitation studies.

Animals↗
PubMedDate not supplied

[Epidemiologic study of acute favism cases (G6PD deficiency) in the Autonomous Community of Murcia during 1983-1987. Report of a case].

This study reminds primary care teams of the fundamental importance of epidemiological histories for diagnosing broad bean disease in rural areas in which broad beans are regularly harvested and the patient's nationality lead one to suspect this illness. A retrospective study of the consumption of broad beans in the region of Murcia is made for the period 1983-1987, the variables of which are: Age, sex, geographical distribution, epidemiological history, diagnosis based on a clinical analysis, need for transfusion, treatment, progress and family case study. Among the conclusions, the greatest number of cases occur in males ranging from 0 to 10 years of age, residents basically of Murcia and the surrounding districts, with a prior history of having ingested broad beans, showing symptoms, revealed through a clinical analysis, which are compatible with are compatible with an hemolytic anemia, for which it was necessary to provide transfusions for a considerable number of cases, achieving good progress and a treatment based on the procedure of fundamentally eliminating broad beans from their diet and certain medicines, and lastly, a later study of the members of these patients' families.

Acute Disease↗
PubMed1987

G6PD deficiency.

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Anemia, Hemolytic↗