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In vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin.

The minimum inhibitory concentrations (MIC) of ciprofloxacin, enrofloxacin, and norfloxacin were tested for approximately ten clinical isolates of each of Actinobacillus pleuropneumoniae, Actinobacillus suis, Actinomyces pyogenes, Corynebacterium pseudotuberculosis, Erysipelothrix rhusiopathiae, Haemophilus parasuis, Haemophilus somnus, Pasteurella haemolytica, Pasteurella multocida, Rhodococcus equi, Streptococcus equi, Streptococcus suis and Streptococcus zooepidemicus. Ciprofloxacin and enrofloxacin had similar activity and were more active than norfloxacin. All isolates had an MIC of 1.0 microgram/mL or less for ciprofloxacin and enrofloxacin, and these drugs had particularly marked activity against the gram-negative bacteria tested.

Actinobacillus↗

[Microbiological and immunodiagnostic studies in experimental erysipeloid].

The use of the passive hemagglutination test with a set of antigenic and antibody erythrocytic diagnostic reagents made it possible to detect antibodies to Erysipelothrix rhusiopathiae specific and type specific antigens in the blood serum of infected mice and to reveal the presence of these antigens in the internal organs of the animals. After a considerable increase of the infective dose a higher level of contamination and the retention of the antigens in the internal organs were observed, while the activity of specific antibodies in the blood serum decreased.

Acute Disease↗

Parvovirus antibodies in vaccinated gilts in field conditions--results with HI and ELISA tests.

This study was conducted to determine the antibody response for porcine parvovirus (PPV) of 39 gilts in field conditions after vaccination. Gilts from four herds endemically infected with PPV were injected twice with a commercial vaccine of inactivated PPV and Erysipelothrix rhusiopathiae. The PPV antibodies were analysed both with haemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) in order to study the agreement between these methods. The possible association between high-antibody titres and reproductive failure (repeat breeding, culling for infertility, < or = 6 piglets born alive) was also investigated. In these study herds, endemically infected by PPV, most gilts (84.6%) had not seroconverted by the age of 6 months. On-field vaccination resulted in a consistent increase of humoral immunity not exceeding the antibody level of 1 : 512 in the majority of gilts in all herds examined. The agreement between ELISA and HI tests was moderate (Spearman's rho = 0.87, kappa = 0.63). The seroconversion over the level >1:512 by mid-pregnancy was not associated with reproductive failure.

Agglutination Tests↗

Infective endocarditis caused by uncommon bacteria.

The present study is an attempt to define the uncommon bacteria (UB) causing endocarditis and to evaluate their prevalence and clinical significance. Review of 13 series (including ours) revealed that UB were the infecting organisms in 105/1989 patients (5.3%). The leading bacteria were: Pneumococcus, Haemophilus sp., Corynebacterium sp., Neisseria sp., Streptobacillus moniliformis, Cardiobacterium hominis and Erysipelothrix sp. These data are similar to those obtained from 111 sporadic case reports published in English during the last 6 yr and cited in the Index Medicus. Infection by UB usually resembles that by the common bacteria. However, Haemophilus sp. seems to affect mainly the young and is associated with major arterial occlusions, and Brucella is claimed to have a greater affinity to the aortic valve. UB are more frequent in patients with polymicrobial infections and their diagnosis is often delayed. Greater awareness to the possibility of infections with UB is needed for early diagnosis and improved prognosis.

Adolescent↗

Bacterial isolation from slaughtered pigs associated with endocarditis, especially the isolation of Streptococcus suis.

Bacterial isolation from slaughtered pigs with endocarditis was carried out from 1985 to 1994. A total of 495 (0.025%) out of 2,006,127 pigs were diagnosed as having endocarditis. Though bacteria were significantly isolated from 399 of the 495 pigs, bacteria could not be isolated in 96 pigs (19.4%). In 11 pigs, 2 bacterial species were isolated from heart lesion. Streptococcus suis was isolated from 127 cases (25.7%), Streptococcus dysgalactiae from 75 (15.2%), Erysipelothrix rhusiopathiae from 63 (12.7%), Actinomyces pyogenes from 39 (7.9%), Pasteurella multocida from 11 (2.2%). Staphylococcus aureus from 10 (2.0%), and Streptococcus porcinus from 8 (1.6%). Among the 99 isolates biochemically identified as S. suis, the major serotype was S. suis type 2 (35.4%). The remainder of the typable isolates were identified as serotypes 1/2 (2.0%) and 9 (1.0%). A total of 61 isolates (61.6%) were untypable.

Abattoirs↗

Evaluation of the microagglutination test in the diagnosis of Mycoplasma gallisepticum infection in chickens.

The sensitivity and specificity of the microagglutination (MA), serum-plate-agglutination (SP), and hemagglutination-inhibition (HI) test for Mycoplasma gallisepticum (MG) were compared in groups of chickens infected with MG, M. synoviae, or Pasteurella multocida or inoculated with bacterins prepared from Staphylococcus aureus or Erysipelothrix rhusiopathiae. Of the three tests evaluated, the HI test had the highest specificity, but it was the least sensitive. Both the MA and SP tests were more sensitive than the HI test but lower in specificity. The MA test was less sensitive than the SP test in detecting antibodies against heterologous MG strains.

Agglutination Tests↗

Two fatal cases of septicaemia due to Erysipelothrix insidiosa.

Clinical, pathological, and necropsy findings are recorded from two cases of endocarditis due to Erysipelothrix insidiosa, one in a 53-year-old grocer and the other in a 56-year-old housewife. The possible sources of infection are suggested.

Agglutination Tests↗

[Erysipelothrix rhusiopathiae--a cause of erysipeloid and endocarditis].

Erysipelothrix rhusiopathiae is widespread among many species of animals including fish. Erysipeloid is the most common infection caused by this bacterium in man; systemic infection, with endocarditis, is rare. Most of the affected patients risk exposure to the organism at work. Two patients with E. rhusiopathiae infections are described; one with erysipeloid after slaughtering a deer, the other with fatal endocarditis after gutting an eel. Erysipeloid may be confused with "seal finger", but this disease probably has a different microbial etiology and requires different antibiotic treatment.

Aged↗

Erysipelas in quail and suspected erysipeloid in processing plant employees.

Erysipelas was diagnosed in two commercial breeder flocks of Coturnix quail and was characterized clinically by sudden mortality. At necropsy, lesions comprised generalized congestion, hemorrhages in the thigh and breast muscles, swelling of liver, spleen, and kidney, and dark red edematous lungs. Erysipelothrix rhusiopathiae was cultured from visceral organs of affected birds. Medication with penicillin in the drinking water controlled the mortality. A possible source of infection was fish raised on the premises. Seven people at the quail processing plant developed localized inflammation of the fingers, which responded when antibiotic therapy for erysipeloid was initiated.

Animals↗

Treatment of surgical infections with a modern quinolone: therapy of soft tissue infections and pneumonia with ofloxacin.

A randomized, open, prospective study including 45 patients with either soft tissue infections (29 cases) or pneumonia (15 cases) after surgical intervention and one case having both indications was carried out with ofloxacin. The standard dose was 200 mg ofloxacin b. i. d. In most cases, clinical symptoms, such as fever, rubor, swelling and pain in soft tissue infections, as well as fever, dyspnoea, physical and radiological signs for pneumonia, subsided within two to seven days. Laboratory data (blood analyses, liver enzymes) remained in the normal range. The overall tolerability was good. Bacteriological controls have proven that ofloxacin is able to eradicate a broad range of pathogens, including persister pathogens and also certain bacteria like Clostridium perfringens and Erysipelothrix rhusiopathiae. In our study, Streptococcus faecalis was of intermediate sensitivity in one case and resistant in another. Ofloxacin has proven to be effective in soft tissue infections and pneumonia after surgical intervention.

Adult↗

In vitro susceptibility of porcine respiratory pathogens to tilmicosin.

Bacterial isolates obtained from swine with various clinical diseases were tested for susceptibility to tilmicosin by minimum inhibitory concentration (MIC) and Kirby-Bauer disk diffusion tests using National Committee on Clinical Laboratory Standards methodology. The tilmicosin MIC90 was < or =0.125 microg/ml for Erysiopelothrix rhusiopathiae, < or = 1 microg/ml for Haemophilus parasuis isolates, 8 microg/ml for Actinobacillus suis and Pasteurella multocida type A, 16 microg/ml for toxigenic and nontoxigenic P. multocida type D, 64 microg/ml for Bordetella bronchiseptica, and >128 microg/ml for Staphylococcus hyicus and Streptococcus suis. The results of disk diffusion testing matched well with the MIC results for each pathogen. This in vitro survey of tilmicosin activity against various swine isolates suggests that further clinical evaluation of tilmicosin in swine may be warranted for disease associated with E. rhusiopathiae, H. parasuis, and A. suis but not B. bronchiseptica, S. suis, or S. hyicus.

Actinobacillus↗