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Possible health hazards from exposure to power-frequency electric and magnetic fields--a COMAR Technical Information Statement.

In recent years concerns have been raised about the biological effects of exposure to electric and magnetic fields at extremely low frequencies (ELF), particularly those associated with the distribution and utilization of electric power. In 1989, the Institute of Electrical and Electronics Engineers (IEEE) issued an "Entity Position Statement" which stated that "there is not enough relevant scientific data to establish whether common exposure to power-frequency fields should be considered a health hazard" and that "there is general agreement that more research is needed to define safe limits of human exposure to power-frequency fields." After examination of relevant research reports published during the last ten years, COMAR concludes that it is highly unlikely that health problems can be associated with average 24-hour field exposure to power frequency magnetic fields of less than 1 microT (10 mG). Good laboratory evidence shows that magnetic fields 100 to 10,000 times higher than this level, either ELF sinusoidal or pulsed, can induce a variety of biological effects, including beneficial health effects such as bone or tissue healing. Many of the reports of effects of weaker fields should be considered preliminary, as some observations have not been reproduced in different laboratories, while others, observed in cells, have not been clearly connected to effects in intact animals. Also, the means of interaction of low-level ELF fields with cells, tissues or laboratory animals is not fully understood; therefore the health impacts of such weak fields on intact animals and humans, if any, cannot be predicted or explained. Further research is needed to confirm or negate reports of effects of weak fields, and to determine mechanisms and relevance of these effects to actual health hazards. Continued study in this complicated area will enhance our understanding of biological systems, as well as help identify levels and types of ELF exposure that may be deleterious to human health.

Animals↗

Inductively coupled plasma emission spectroscopic and flame photometric analysis of goat epididymal fluid.

AIM: The elemental composition of the epididymal luminal fluid (ELF) in adult goat (Capra indica) was investigated. METHODS: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elemental contents was analyzed with inductively coupled plasma (ICP) emission spectroscopy, a microanalytical technique that can simultaneously measure many elements in minute volumes of sample. The Na and K concentrations were determined by flame photometry. RESULTS: ICP spectroscopy showed the presence of copper, calcium, nickel, iron, magnesium, chromium, titanium and zinc in ELF, with fluctuating levels at different sites along the length of the epididymis. Cadmium, cobalt, lead and manganese were not found. The Na+/K+ ratio was seen to be higher at the initial segments of the epididymis and lower at the distal. CONCLUSION: It is proposed that the observed characteristic distribution of elements in ELF may have far reaching implications in sperm maturation and storage known to occur in the epididymis.

Animals↗

Intrapulmonary pharmacokinetics of ofloxacin in drug-resistant tuberculosis.

OBJECTIVE: To determine the lung epithelial lining fluid (ELF) and serum levels of ofloxacin in drug-resistant tuberculosis patients during treatment. DESIGN: Ten drug-resistant tuberculosis patients treated with ofloxacin containing regimens for at least 2 weeks were enrolled in the study. Subjects ingested ofloxacin 10 mg/kg and other anti-tuberculosis agents after overnight fasting. RESULTS: Serum and bronchoalveolar lavage fluid were collected at 4 hours after treatment and assayed by high performance liquid chromatography. The mean concentrations of ofloxacin in serum and ELF were 5.889 +/- 1.096 and 16.583 +/- 8.697 mg/L, respectively. The mean ratio of ELF-to-serum ofloxacin concentration was 2.825 +/- 1.275. CONCLUSION: Ofloxacin can penetrate well into the intra-alveolar fluid of patients treated for drug-resistant tuberculosis. The lung ELF concentrations were consistently higher than the minimal inhibitory concentrations of Mycobacterium tuberculosis as determined in vitro.

Adult↗

Measurement of radiated electromagnetic field levels before and after a changeover to energy-efficient lighting.

An energy-efficient lighting retrofit at the Food and Drug Administration (FDA) Winchester Engineering and Analytical Center (WEAC) presented the opportunity to measure the electromagnetic (EM) environments in several rooms before and after changing the fluorescent lighting systems and to compare the changes in EM fields with the proposed standard EM immunity levels. Three rooms, representing the types of work areas in the laboratory, were selected and measured before and after the lighting changeover. Electric and magnetic field measurements were taken in the extremely low frequency (ELF), very low frequency (VLF), and radio frequency (RF) ranges of the EM spectrum. In 2 rooms, ELF electric fields were reduced and VLF and RF electric fields were increased as a result of the changeover to high-frequency fixtures. A third room received low-frequency, energy-efficient fixtures during this changeover, and this change resulted in only a slight increase of the ELF electric fields. The ELF magnetic fields were greatly reduced in 2 but only slightly reduced in the third room. No significant change was seen in VLF or RF magnetic fields for any of these rooms. Some field-strength measurements exceeded the proposed immunity levels recommended in the draft International Electrotechnical Commission standard IEC 60601-1-2 (rev. 2). The data show that increasing the separation distance from the fluorescent light fixtures greatly reduces the field-strength levels, limiting the potential for EM interference.

Electromagnetic Fields↗

Levels of interferon-gamma and interleukin-2 receptor-alpha for bronchoalveolar lavage fluid and serum were correlated with clinical grade and treatment of pulmonary tuberculosis.

OBJECTIVE: We investigated possible correlations for interferon-gamma (IFN-gamma) and soluble interleukin-2 receptor-alpha (sIL-2R-alpha) levels in bronchoalveolar lavage fluid (BALF), and clinical grade of pulmonary tuberculosis (TB), which is determined by factors such as extent of pulmonary involvement, fever and loss of body weight. DESIGN: In order to explore these correlations and address associated questions, BALF was collected from 45 patients presenting with active pulmonary TB and 14 healthy controls. Repetitive BALF was collected in 17 patients after 3 months of anti-tuberculosis chemotherapy. The epithelial lining fluid (ELF) levels for IFN-gamma and sIL-2R-alpha were measured using enzyme-linked immunosorbent assay (ELISA) after standardization with urea. RESULTS: Patients with higher-grade pulmonary TB (i.e., with more advanced pulmonary involvement, fever or body weight loss), revealed significantly higher ELF levels for IFN-gamma and sIL-2R-alpha compared to those with lower grade pulmonary TB. Similar results were also determined for sIL-2R-alpha serum levels, but not for IFN-gamma serum levels. After anti-tuberculosis chemotherapy the elevated cytokine levels for ELF and serum significantly decreased in accordance with radiographic improvement. CONCLUSIONS: ELF levels of IFN-gamma and sIL-2R-alpha were correlated with disease grading of pulmonary TB and decreased after anti-tuberculosis chemotherapy.

Bronchoalveolar Lavage Fluid↗

[Abnormal shift of connexin 43 gap-junction protein induced by 50 Hz electromagnetic fields in Chinese hamster lung cells].

OBJECTIVE: To study the effects of extremely low frequency magnetic fields(ELF MF) on the amount and localization of connexin 43(Cx43) gap-junction protein in the Chinese hamster lung(CHL) cells, and to explore the mechanism of ELF MF suppression on gap-junctional intercellular communication(GJIC). METHODS: The cells were irradiated for 24 h with 50 Hz sinusoidal magnetic field at 0.8 mT without or with 12-O-tetrade-canoylphorbol-3-acetate(TPA), 5 ng/ml for 1 h. The localization of Cx43 proteins were performed by indirect immunofluorescence histochemical analysis and detected by confocal microscopy. The second experiment was conducted to examine the quantity of Cx43 proteins level in nuclei or cytoplasm and detected by Western blotting analysis. RESULTS: The cells exposed to TPA for 1 h displayed less bright labelled spots in the regions of intercellular junction than the normal cells. Most of Cx43 labelled spots occurred in the cytoplasm and aggregated near the nuclei. At the same time, the amount of Cx43 protein in cytoplasm were increased[(2.03 +/- 0.89) in ELF group, (2.43 +/- 0.82) in TPA group] as compared to normal control(1.04 +/- 0.17) (P < 0.01). CONCLUSION: Inhibition on GJIC function by ELF MF alone or combined with TPA may be related with the shift of Cx43 from the regions of intercellular junction to the cytoplasm.

Animals↗

Acute effects of low-frequency electromagnetic fields on leukocyte-endothelial interactions in vivo.

BACKGROUND: To clarify the effect of extremely low frequency electromagnetic field (ELF-EMF) on the microcirculatory system, acute effects on leukocyte-endothelium interactions resulting from ELF-EMF exposure were examined with conscious BALB/c mice by means of a dorsal skinfold chamber. MATERIALS AND METHODS: The fluorescent dye, rhodamine 6G, was injected into the caudal vein to visualize leukocytes in vivo and to analyze leukocyte-endothelium interactions. Mice were exposed in vivo to 50 Hz linear sinusoidal wave EMF at magnetic flux densities of 3, 10 or 30 mT, for 30 minutes. RESULTS: The mean velocity of free flowing leukocytes in the center stream of venules revealed no distinct changes following exposure. However, at a flux density of 30 mT, the number of adherent leukocytes, defined as the total number of rolling and endothelium-adhering leukocytes, increased significantly following ELF-EMF exposure (p<0.05); no significant differences in adherent cell numbers were found in the 3 mT and 10 mT exposure groups or sham controls comparing pre- and post-exposure periods. CONCLUSION: These results demonstrated that high magnetic flux densities of ELF-EMF had an effect on leukocyte-endothelium interactions and indicated that a threshold level for this phenomenon exists in the range of 10-30 mT under these experimental conditions.

Animals↗

Post-treatment changes of six cytokines in active pulmonary tuberculosis: differences between patients with stable or increased fibrosis.

SETTING: Little information is available regarding the relationship between the fibrotic evolution of pulmonary tuberculosis (PTB) and cytokine levels in human bronchoalveolar lavage fluid (BALF) and serum. OBJECTIVE: To evaluate correlations between profibrotic cytokine levels and post-treatment lung fibrotic evolution. DESIGN: BALF and serum amounts of pro- or anti-inflammatory cytokines were obtained by ELISA before and 6 months after the start of anti-tuberculosis chemotherapy in 13 subjects with PTB. BALF levels were recalculated as ELF (epithelial lining fluid) levels by the urea method. High resolution computed tomography (HRCT) of both lungs was performed at the same time. RESULTS: When comparing pre- and post-treatment radiological data, the scores for 2-10 mm nodules, consolidation and fibrosis presented significant differences (P < 0.05). Concomitantly, pre-treatment vs. 6 month concentrations of ELF IFN-gamma and TNF-alpha were decreased (P < 0.05), while those of IL-4 and IL-10 were increased (P < 0.012). At serum level, IFN-gamma decreased, as did TNF-alpha, TGF-beta1 and PDGF-BB (P < 0.05). When the patients were subdivided into two groups, 1) stable or 2) increasing HRCT fibrosis score, significant increases in the second group were observed for ELF/ serum values of TGF-beta1 and ELF PDGF-BB (P < 0.05) at 6 months post-treatment. Only serum TGF-beta1 values were significantly associated with the same group before treatment.

Adult↗

Azithromycin concentrations at the sites of pulmonary infection.

Azithromycin is a new macrolide antimicrobial. The distribution to the potential sites of pulmonary infection was assessed after the administration of a single 500 mg oral dose to 22 patients undergoing fibreoptic bronchoscopy. Concentrations of azithromycin in sputum, bronchial mucosa, eptihelial lining fluid (ELF) and alveolar macrophages (AM) were determined at intervals up to 96 h after dosing. The mean serum concentration was low at 12 h (0.13 micrograms.ml-1, SEM 0.05) but was still detectable at 96 h (0.01 micrograms.ml-1). In contrast, peak sputum ELF, bronchial mucosal and AM levels were found at 48 h. Bronchial mucosal concentrations were significantly greater than ELF concentrations, which were in turn greater than sputum concentrations. Mean peak AM concentrations were sixfold greater than bronchial mucosal concentrations (23 micrograms.ml-1, SEM 5.1 and 3.89 micrograms.ml-1, SEM 1.2, respectively). The high intracellular concentrations indicate that azithromycin is likely to be effective for sensitive intracellular pathogens and the favourable penetration into sputum, ELF and bronchial mucosa suggest that it should be useful in pneumonia and bronchial infections.

Azithromycin↗

Regulatory steps in the initiation of protein synthesis.

Regulation of protein synthesis is being exerted at different levels with a different extent of attenuation. The major control module seems to work by the inactivation of the elF-2 recycling which enables the cell to shift down from a high rate of initiation to a low rate. Certain events in the cell cycle like mitosis do show such a drastic change in initiation rate. It is suggested that modifications of elF-2 by phosphorylation of the alpha-subunit by different protein-kinases is the basis for such a control mechanism. Already two protein kinases of this type have been described, the hemin-regulated inhibitor and the ds-RNA activated inhibitor from interferon-treated cells. On the other hand modifications of the beta-subunit by other metabolic events, for instance low NADH/NAD+ ratio, can as yet not be excluded. Other conditions like amino acid starvation, serum deprivation, heat-shock and virus-infection seem to evoke quite different strategies. In some cases it has been demonstrated that inactivation of mRNA binding factors as elF-4B and elF-4E, favour the translation of low-dependence, i.e. low secondary structure, messengers. It shall be worthwhile to establish whether the mRNA's with such low degree of secondary structure encoded proteins that are aimed at the survival of the cell under extreme metabolic or stress conditions. Much more work on the structure and nucleotide sequences of the leader sequence is needed to prove these hypothetical points.

Kinetics↗

[Very low frequency electric and magnetic fields and the immune system].

Recent residential and occupational epidemiological studies indicate a statistical association between 50-60 Hz magnetic field exposure and the risk of developing some kinds of tumors. Several experimental researches have been carried out in vitro and in vivo to verify the possibility that some cell functions may be influenced by ELF (Extremely Low Frequencies: 0-300 Hz) electric and magnetic fields. Such researchers are very important to assess if the statistical association indicated by the epidemiological studies is actually due to a cause-effect relationship between ELF electric and magnetic fields and carcinogenesis. In this review we describe the present state of the experimental research, focusing our attention on the effects of ELF fields on the immune system. We also describe some theoretical researches whose aim is to identify possible mechanisms of interaction between ELF fields and biological systems which may provide biological plausibility to the observed effects.

Animals↗

Dynamics of soluble adhesion molecule levels in patients with pollinosis.

OBJECTIVES: To define the dynamics of systemic and local soluble adhesion molecule levels and to discuss the role of these molecules in the pathogenesis of allergic rhinitis. DESIGN: Randomized control trial. SUBJECTS: Twelve volunteers with Japanese cedar pollinosis and 7 healthy volunteers. INTERVENTIONS: The levels of soluble intercellular adhesion molecule 1 (sICAM-1), soluble vascular cell adhesion molecule 1 (sVCAM-1), soluble E-selectin, and soluble L-selectin in serum samples and nasal epithelial lining fluids (ELF) from 12 patients with pollinosis were measured 5 times throughout the allergy preseason to postseason, and the results were compared with those from 7 healthy subjects. RESULTS: The levels of sICAM-1 (P < .05) and sVCAM-1 (P < .05) in sera were up-regulated, and the levels of soluble L-selectin (P < .01) in sera were down-regulated during the early stage of the season in the allergic subjects. The difference between the levels of sICAM-1 and sVCAM-1 in sera in the early and mid-season was statistically significant in the allergic subjects (P < .05). The levels of sICAM-1 in ELF were up-regulated during the early and mid-season. The levels of sVCAM-1, soluble E-selectin, and soluble L-selectin in ELF were undetectably low throughout the preseason to postseason. CONCLUSION: There is evidence of the unique stage-dependent differential contributions of various soluble adhesion molecules in the pathogenesis of seasonal allergic rhinitis with a small amount of natural allergen provocation.

Adult↗

Male proportion in offspring of parents exposed to strong static and extremely low-frequency electromagnetic fields in Norway.

Reduced male proportion in offspring of male carbon setters prompted a study into whether offspring of workers exposed to strong static and extremely low-frequency electromagnetic fields (ELF) had a deviant sex ratio. The study was based on all births in Norway 1970-1993. The reference population was offspring of parents not exposed to ELF. The male proportion in offspring of men in aluminum works was 50.38%, RR 0.98 (0.94-1.03), in manganese works 47.32%, RR 0.92 (0.83-1.02), in factories producing iron 50.03%, RR 0.97 (0.93-1.02), in nickel works 48.27%, RR 0.94 (0.84-1.05), and in electric wire production 47.20%, RR 0.92 (0.80-1.05). In the offspring of women in aluminum works, the male proportion was 37.04%, RR 0.72 (0.59-0.90), in all smelter works grouped together, 45.13%, RR 0.88 (0.79-0.99). The male proportion in the reference population was 51.42%. The male proportion in offspring of men in industries with ELF, was slightly reduced, while offspring of women was significantly reduced.

Aluminum↗

Effect of low frequency, low amplitude magnetic fields on the permeability of cationic liposomes entrapping carbonic anhydrase: II. No evidence for surface enzyme involvement.

Observations recently reported by our group indicate that combined 7 Hz sinusoidal (B(acpeak) = 50 mu T) and parallel static (B(dc) = 50 mu T) magnetic fields can induce a significant increase in diffusion rate of substrate across carbonic anhydrase (CA)-loaded liposomes (DPPC:Chol:SA). A direct involvement of charges of stearylamine (SA) on the lipid membrane surface was also demonstrated. Kinetic studies showed that CA was mainly entrapped in liposomes at 5:3:2 molar ratio, although a small amount (17%) of enzyme was also located on the external surface of these cationic liposomes. In this paper we report steady state kinetic studies on this latter CA after ELF-EMFs exposure. No difference in the apparent K(m) between exposed and sham samples was observed. On the contrary the apparent V(max) was increased by approximately a factor of 2 after field exposure. In spite of the proteolytic digestion of this external CA, a significant increase of enzymatic activity, as a function of increase in the diffusion rate of substrate across the lipid bilayer, was observed in the exposed samples. Based on these results, a conformational change induced by the field on the CA located on the external surface of 5:3:2 liposomes is excluded as an explanation for our previous observations, supporting the primary role of bilayer SA in the interaction with ELF. A model of ELF interaction, based on the Larmor precession theory, explaining the physical phenomenon induced on the dipole of SA has been developed.

Animals↗

Epidemiological studies of work with video display terminals and adverse pregnancy outcomes (1984-1992).

Many epidemiological studies have investigated the claim that work with video display terminals (VDT) is a risk factor during pregnancy. Results have been inconsistent, although in the majority of cases the hypothesis was not supported. Exposure assessment has been very poor and the statistical power of the studies generally low. Overall, the studies indicate that VDT operators are not at greater risk than the general population, because very low frequency (VLF) magnetic fields do not appear to be a risk factor and extremely low frequency (ELF) magnetic field exposure is not significantly greater than that experienced in other occupational and residential environments. However, since some studies lend support to the hypothesis that ELF magnetic fields may be a risk factor for pregnancy outcome, studies of subjects exposed to higher than average ELF fields are justified.

Abortion, Spontaneous↗

No effects of extremely low frequency magnetic fields found on cytotoxic activities and cytokine production of human peripheral blood mononuclear cells in vitro.

Several epidemiologic studies have suggested an association between exposure to extremely low frequency (ELF) magnetic fields (MFs) and cancer in adults and children. A possible target of MFs is the immune system. The effects of the exposure to ELF MFs on the immunological functions of human peripheral blood mononuclear cells (PBMCs) obtained from healthy male volunteers were assessed by measuring the natural killer (NK) and lymphokine activated killer (LAK) activities and the production of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2), and interleukin-10 (IL-10). The PBMCs were exposed to three different MF: linearly polarized (vertical), circularly polarized, and elliptically polarized, at 50 and 60 Hz. Magnetic flux densities were set at 500, 100, 20, and 2 microT (rms) for vertical field and at 500 microT (rms) for the rotating fields. Using cytotoxicity assay and enzyme-linked immunosorbent assay (ELISA) for cytokine production, we could not find any effects of ELF MFs on the cytotoxic activities and the cytokines production of human PBMCs.

Adult↗

Extracellular currents alter gap junction intercellular communication in synovial fibroblasts.

We studied the effect of extremely low frequency (ELF) currents on gap junction intercellular communication (GJIC) mediated by connexin43 protein. Confluent monolayers of synovial fibroblasts (HIG-82) and neuroblastoma cells (5Y) were exposed in bath solution to 0-75 mA/m(2) (0-56 mV/m), 60 Hz. Single channel conductance, cell membrane current-voltage (I-V) curves, and Ca(2+) influx were measured using the nystatin single and double patch methods. The conductances of the closed and open states of the gap junction channel in HIG-82 cells were each significantly reduced (by 0.76 and 0.39 pA, respectively) in cells exposed to 20 mA/m(2). Current densities as low as 10 mA/m(2) significantly increased Ca(2+) influx in HIG-82 cells. No effects were seen in 5Y cells. The I-V curves of the plasma membranes of both types of cells were independent of 60 Hz electric fields and current densities, 0-75 mA/m(2), indicating that the effect of the 60 Hz fields on GJIC in HIG-82 cells was not mediated by a change in membrane potential. We conclude that ELF electric fields can alter GJIC in synovial cells via a mechanism that does not depend on changes in membrane potential, but may depend on Ca(2+) influx. The results open the possibility that GJIC mediated responses in synovial cells, such as for example, their secretory responses to proinflammatory cytokines, could be antagonized by the application of ELF electric fields.

Animals↗

Alternating extremely low frequency magnetic field increases turnover of dopamine and serotonin in rat frontal cortex.

The aim of this study was to evaluate the influence of an extremely low frequency sinusoidal magnetic field (ELF MF) with frequency of 10 Hz and intensity of 1.8-3.8 mT on the levels of the biogenic amines dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-methoxytyramine (3-MT), 5-hydroxytryptamine (5-HT), 5-hydroxyindolacetic acid (5-HIAA), and noradrenaline (NA), as well as on DA and 5-HT turnover in corpus striatum and frontal cortex of adult male Wistar rats. We found that ELF MF exposure for 14 days, 1 h daily, did not influence the level of the examined biogenic amines and metabolites, but increased the rate of synthesis (turnover) of DA and 5-HT in rat frontal cortex as compared to control, sham exposed rats. On the basis of the present results and our previous findings, extremely low frequency magnetic field (ELF MF) exposure has been found to alter both turnover and receptor reactivity of monoaminergic systems, as well as some behaviors induced by these systems or their agonists and antagonists.

Animals↗