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Experimental analysis of character coupling across a complex life cycle: pigment pattern metamorphosis in the tiger salamander, Ambystoma tigrinum tigrinum.

Developmental relationships among characters are expected to bias patterns of morphological variation at the population level. Studies of character development thus can provide insights into processes of adaptation and the evolutionary diversification of morphologies. Here I use experimental manipulations to test whether larval and adult pigment patterns are coupled across metamorphosis in the tiger salamander, Ambystoma tigrinum tigrinum (Ambystomatidae). Previous investigations showed that the early larval pigment pattern depends on interactions between pigment cells and the lateral line sensory system. In contrast, the results of this study demonstrate that the major features of the adult pigment pattern develop largely independently of both the early larval pattern and the lateral lines. These results suggest that ontogenetic changes that occur across metamorphosis decouple larval and adult pigment patterns and could thereby facilitate independent evolutionary modifications to the patterns during different stages of the life cycle.

Ambystoma↗

Effects of infection of common root rot on protein content, cooking quality and other characters in pea varieties.

Pea varieties are known to show a considerable variability in seed yield, protein content, cooking quality and other characters over years and locations. Common root rot, a wide spread soil borne disease of peas in Europe and elsewhere, is a contributing factor in the observed variation in pea yields and pea quality characteristics such as protein content and cooking quality. It interacts with the weather and these factors together constitute important reasons for the variations observed within a single variety in the mentioned characters. The common root rot is, however, in seasons with more moderate deviations from the mean in terms of temperature and precipitation, itself able to constitute the most important source of variation in characteristics of the harvested crop. During the 1980ies and the 1990ies investigations were carried out to study the interactions between common root rot (Aphanomyces euteiches) and peas (Pisum sativum). Seed yield, protein content, cooking quality, hectolitre weight, thousand kernel weight, growing time, duration of flowering time and stem length were all significantly influenced by the fungus.

Cooking↗

Impact of character correlation and variable groupings on modern human population tree resolution.

Relationships among modern human populations are often explored through the use of linear measurements taken on the cranium and expressed in the form of dendrograms. However, craniometric variables are strongly correlated and thereby violate the assumption of independence that most statistical analyses require. This study explores the relationship between differing methods of variable treatment and the statistical robustness of the outcomes they yield, as depicted in interpopulational trees of relatedness among modern humans. Three methods of grouping variables are examined. The first method leaves them ungrouped, the second groups variables on the basis of the developmental and/or functional complex of the cranium to which they are thought to belong, and the last method reduces variables by using principal components analysis. The strength of each of these methods is tested through the use of the Continuous Character Maximum Likelihood (CONTML) program in the PHYLIP phylogeny inference package. This program produces output in the form of trees, and the resolution of the branching topology is given as a log-likelihood value, with statistical confidence intervals supporting each branch placement on the tree. The results indicate that leaving variables ungrouped provides misleadingly strong results by failing to account for character correlation. Of the alternative two grouping methods, the covarying components method yields the best-resolved tree with stronger statistical support for its topology than the approach of grouping variables on the basis of their location on the cranium. Finally, the implications for interpreting population histories based on such methods are discussed.

Adolescent↗

Effects of altitude, ethnicity-religion, geographical distance, and occupation on adult anthropometric characters of eastern Himalayan populations.

With a view to estimating the effect of altitude on body dimensions vis-à-vis ethnicity-religion, geographical distance, and occupation, a comprehensive multivariate statistical analysis was performed on data pertaining to 16 anthropometric characters collected from 1,103 individuals (643 males and 460 females) belonging to two ethnic groups-Sherpa and Lepcha. Samples were drawn from several locations in the eastern Himalayan region-Darjeeling and Kalimpong in West Bengal (India), and Nepal, situated at low (1,000-2,000 meters) and high (above 3,500 meters) altitudes. The individuals sampled practice different occupations and follow different religions. Significant age and sex effects were observed. The data were age-adjusted, and sexes were treated separately. A test of equality of mean vectors indicated heterogeneity among population groups. Almost all characters were found to contribute significantly to the ability to discriminate between the groups. The overall probability of correctly classifying an individual based on body dimensions into the group in which she or he actually belongs was high (between 0.64 and 0.77). Shape and size factors could be identified that explained about 50% of the total variance and yielded a reasonable separation of the groups. Results of four different types of multivariate statistical analyses were in agreement, and showed that altitude is most highly associated with body dimensions.

Adult↗

New characters for the functional interpretation of primate scapulae and proximal humeri.

The study of muscle function in nonhuman primates through the technique of electromyography (EMG) has facilitated the identification of specific functional roles for muscles in particular behaviors. This has led to a more complete understanding of the biomechanics of certain regions of the musculoskeletal system, and should facilitate our ability to identify morphological features useful in the functional interpretation of fossil material. The current paper represents one such investigation of a new set of morphometric characters of the scapula and proximal humerus suggested by EMG analyses of shoulder muscle function. A set of new metric variables were examined on the scapulae and proximal humeri of 25 species of extant anthropoid primates, as well as on casts of scapulae and humeri of three fossil primate taxa. The variables are primarily related to the line of action and attachments of the rotator cuff muscles. The position of the scapular spine, the degree of lateral expansion of the subscapular fossa, the size and shape of the subscapularis insertion facet on the lesser tubercle, and the orientation of the infraspinatus insertion facet on the greater tubercle all appear to successfully sort the extant taxa into locomotor groups. Their appearance on the fossil specimens generally supports previous functional interpretations of each taxon's locomotor abilities based on a variety of other characters, suggesting that these traits are equally applicable to fossil material.

Animals↗

The structure of cruciforms in supercoiled DNA: probing the single-stranded character of nucleotide bases with bisulphite.

The single-stranded character of cytosine bases in three cruciform structures has been assessed by an examination of reactivity towards sodium bisulphite. Unpaired cytosine residues undergo deamination at C4 to give deoxyuracil, and propagation in an ung Escherichia coli host results in C-G----T-A transition mutations, detectable by restriction cleavage or sequence analysis. Very high frequencies of such mutations have been found at cruciform loops, confirming their unpaired character, with almost zero background mutation frequencies elsewhere. A low level of modification was observed at the four-way junction of a cruciform. The results indicate that the optimal cruciform loop size is four bases, with loose 'breathing' at the first base pair at the top of the cruciform stem at 37 degrees C, and little or no opening of base pairs at the four-way junction.

Base Sequence↗

Evolutionary transformations of chorioallantoic placental characters in rodentia with special reference to hystricognath species.

The aim of this paper is to reconstruct the evolution of chorioallantoic placental characters in Rodentia. The analysis is based on pre-existing hypotheses of rodent relationships and the tracing of character evolution. Data on 64 rodent species of 49 genera are derived from the literature. New results refer to the hystricognath species Petromus typicus A. Smith, 1831 and Octodon degus (Molina, 1782). This comprehensive analysis confirms that the stem species pattern of Rodentia is characterised by a haemochorial placenta which is divided horizontally. Inside the placental labyrinth, fetal vessels and their trophoblastic external border build up a network through which the maternal blood flows. The trophoblastic tissue is one-layered, syncytial and possess a considerable surface extension. Within Rodentia, evolutionary transformations occurred on the macroscopic as well as the fine structural level. The results suggest that the stem species of Hystricognathi underwent transformations only on the macroscopic level, i.e., forming a ring-shaped arrangement of placental regions with centrally situated maternal arteries and the acquisition of a subplacenta. By contrast, in Muridae the chorioallantoic placenta shows derived features only in regard to the fine structure of the labyrinth, i.e. the interhaemal membrane is modified in composition, and the fetal capillary endothelium is fenestrated. Geomyoidea underwent transformations on both levels. Macroscopically, their placenta is modified into a hillock shape. Microscopically, the interhaemal membrane is formed by the cytotrophoblast. In addition to the mentioned transformations, some aspects of other fetal membrane differentiation in Rodentia are briefly discussed.

Allantois↗

Sabertooth characters in the clouded leopard (Neofelis nebulosa Griffiths 1821).

The clouded leopard (Neofelis nebulosa) is an unusual medium-sized felid whose ecology in the wild is poorly known. Mainly famous for its large canines, it has often been an overlooked taxon in analyses of felid morphology and systematics or has proven aberrant and difficult to interpret. In this article I report on a number of unusual features in the clouded leopard skull hitherto considered characteristic of sabertooth felids exclusively, and, accordingly, universally believed to be absent in extant felids. The skull morphology of the clouded leopard sets it apart from other extant felids, and in a number of respects it approaches the morphology of primitive sabertooths. This indicates convergence of several characters in machairodontine felids and the clouded leopard, mainly as adaptations for attaining a large gape. This raises doubts about the characters hitherto considered as distinguishing sabertoothed from nonsabertoothed predators, and since no evidence at present suggests a different functional killing and feeding ecology in Neofelis, regardless of its unusual skull morphology, also whether primitive sabertoothed felids were functionally similar to advanced forms such as Homotherium, Megantereon, or Smilodon.

Animals↗

Differential predation cost of a secondary sexual character: sparrowhawk predation on barn swallows.

Models of reliable signalling assume that secondary sexual characters and other displays are more costly to individuals of low than high phenotypic quality, and that low quality individuals disproportionately compromise their reproduction and survival prospects by investment in signalling. A field study of barn swallows, Hirundo rustica with sexually exaggerated tail feathers, supported this prediction. Males were captured by sparrowhawks, Accipiter nisus more often than females, and captured males had shorter and more asymmetric tails than male barn swallows that were still alive at the end of the breeding season. These results suggest that there was a negative relationship between degree of sexual ornamentation and predation risk, consistent with the hypothesis that the secondary sexual character is a reliable indicator of quality.Copyright 1997 The Association for the Study of Animal Behaviour

Journal Article↗

Determinants of the thirst-quenching character of beer.

The purpose of this study was to find which sensory characteristics are significant determinants of the thirst-quenching character of beer. The sensory attributes and thirst-quenching characteristics ("thirst quenching", "refreshing", "drinkability") of 18 beers were evaluated by a panel of 12 judges. The only significant positive determinants of the "thirst-quenching" character of the beers in the design were carbonation and bubble density. Significant negative determinants were foam, overall aroma and flavor, color, viscosity, malty, hoppy, burnt, bitterness, acidic, metallic, astringency and aftertaste. The same correlations (both in terms of direction and significance level) were observed between descriptive attributes and the "refreshing" and "drinkability" factors. A principal component analysis (PCA) of the mean ratings indicated that variability among beers was mostly along a thirst-quenching dimension (72% of the variance). Procrustes analysis performed on the individual ratings showed that, for one of the judges, the interpretation of the thirst-quenching concept differed from that shown by PCA for the overall panel.

Adult↗

Effects of implantation of hypertrophied androgenic glands on sexual characters and physiology of the reproductive system in the female red claw crayfish, Cherax quadricarinatus.

The role of the androgenic gland (AG), an organ unique to male Crustacea, in the development of sex characters and physiology of the reproductive system has not been fully documented in the red claw crayfish, Cherax quadricarinatus. To investigate the role of the AG in this species, the effect of implanting hypertrophied AGs into immature female animals was followed. Of the female animals with AG implants, 91.6% developed male-like propodi, including the red patch characteristic of males of this species. The development of female secondary sex characteristics such as a wider abdomen, a wider endopod, and simple setation was inhibited. At the end of the experiment, the ovaries of the AG-implanted females contained mostly lipid-stage oocytes, with a small number of oocytes at the early yolk stage. The gonadosomatic index of the AG-implanted females was significantly lower than that of the control (sperm duct-implanted or sham-operated) females, which had mature oocytes with a well-defined perinuclear zone and yolk globules. An immunohistochemical test using an antibody developed against a 106-kDa secondary vitellogenic polypeptide showed only slight immunoreactivity in the oocytes of AG-implanted females compared with abundant immunoreactivity in control ovaries. In the polypeptide profile of the high-density lipoprotein (HDL) from the hemolymph of AG-implanted females, the 206- and 79-kDa secondary vitellogenesis-specific polypeptides were not found, whereas they were present in the profile of control females. In contrast, the female-specific 177-kDa polypeptide was present in the polypeptide profile of hemolymph HDL of both AG-implanted females and control females. It seems therefore that while secondary sex characters were masculinized under the influence of the implanted AG, the process of vitellogenesis was suppressed but not fully eliminated in the AG-implanted females.

Androgens↗

Influence of the Fractal Character of Model Substances on their Reactivity at Solid-Liquid Interfaces.

The fractal theory has been applied to study surface complexity where surface aspects influence physical properties and play a role in controlling heterogeneous reactions at interfaces. In this work, the influence of the fractal character of some selected antacids was investigated in regard to their neutralizing activity. The materials used were magnesium trisilicate, magnesium hydroxide, and heavy and light magnesium oxide, each from three different manufacturers. Surface area, total pore volume, and particle size were measured. Fractal dimension was determined from gas adsorption data according to pore size distribution, the Frenkel-Halsey-Hill, and thermodynamic methods. The results obtained show a correlation between neutralization activity and fractal character rather than total surface area or particle size. Also, the effect of porosity in terms of total pore volume was modified by the structure of the porous network. The complexity of the pore network played a major role in controlling reactivity. However, the effect of surface roughness was only demonstrated when adsorption on the surface was a rate-limiting step in the reaction. Copyright 1999 Academic Press.

Journal Article↗

The Fixed Sample Search Rule and Use of an Indicator Character to Evaluate Mate Quality.

The phenotypic correlation observed between mated males and females is a direct result of the decision rules that individuals employ during search for a mate. We generalise the fixed sample search rule and examine how the functional relationship between a male indicator character and the fitness benefit of a mating decision influences female search behaviour. If the phenotypes of males and females combine additively to influence the benefit of a mating decision and fitness is a highly inclined function of phenotypic values the optimal value of n, the number of males that females sample during search, is relatively large. The phenotype of the searcher has no impact on the optimal value of n if fitness is a linear function; the optimal n increases with the phenotype of females if the fitness function is convex; and a concave fitness function induces females with high trait values to sample relatively few males. Because females that sample many potential mates are expected to encounter a male with a high expression of the indicator trait linear, convex and concave fitness functions induce a random, assortive and disassortative combination of phenotypes between mated individuals, respectively. Thus, the fixed sample research tactic can produce the variety of phenotypic correlations observed between mated individuals in natural systems and our ability to derive rule-specific predictions of search behavior may generally require information on how characters used to evaluate potential mates are related to fitness.Copyright 1998 Academic Press

Journal Article↗

Ecological character displacement in quantitative genetic models.

We study, both analytically and numerically, models of ecological character displacement for two species that compete for the same set of food sources. These models include quantitative genetics and Lotka-Volterra type competition and are symmetric with respect to the two species. We allow for various shapes of the carrying capacity and the competition function, and we discuss under what general conditions large character displacement can occur. While some of these conditions, like genetic rigidity, or flat and truncated carrying capacity curves, were known before, we also find that slow dynamics of the genetic variance, steep slopes in the interaction function and carrying capacities that are not truncated can lead to large displacements. We interpret these conditions biologically and also give new insights into models which have been previously investigated.

Animals↗

Phylogeny and character evolution in the Empidonax group of tyrant flycatchers (Aves: Tyrannidae): a test of W. E. Lanyon's hypothesis using mtDNA sequences.

We sequenced mitochondrial DNA from four protein-coding genes for 26 taxa to test W. E. Lanyon's hypothesis of intergeneric relationships and character evolution in the Empidonax group of tyrant flycatchers. Three genera in this group (Empidonax, Contopus, and Sayornis) successfully occupy north temperate habitats for breeding, while the remaining genera (Mitrephanes, Cnemotriccus, Aphanotriccus, Lathrotriccus, and Xenotriccus) are restricted to neotropical latitudes. Lanyon hypothesized two major clades in the group based on differences in syringeal morphology and proposed relationships among genera using a combination of morphologic, behavioral, and allozymic characters. The mtDNA data strongly support Lanyon's division of genera into two clades. In addition, the molecular and nonmolecular data sets agree in uniting Aphanotriccus and Lathrotriccus as sister taxa, with Cnemotriccus as basal to these genera. Species of Aphanotriccus, Lathrotriccus, and Cnemotriccus form a clade that exploits a distinctive nesting niche relative to other members of the Empidonax group. Within the second major clade, mtDNA sequences support a reconstruction based on allozymes that places Contopus and Empidonax as sister taxa. This hypothesis contradicts that of Lanyon, who allied Contopus with Mitrephanes on the basis of similarity in foraging mode. Genera in the Empidonax group are members of a larger assemblage that radiated in South America. Occupancy of temperate habitats by certain genera in this group is coincident with their evolution of migratory behavior and with independent diversification in foraging modes that reduces potential competition in sympatry.

Cytochrome b Group↗

Activation of lateral extrastriate areas during orthographic processing of Japanese characters studied with fMRI.

We investigated the early orthographic processing in the occipital cortex using Japanese morphogram by functional magnetic resonance imaging. Kanji (Japanese morphogram) is one system of character used in the Japanese language, each character of which has a specific meaning and pronunciations. To ensure that the effects of the general visual properties of Kanji were excluded from Kanji-related activation, we created strict control stimuli, the "scrambled Kanji" that had the same luminance, contrast, and retinotopical size as those of the original Kanji. In the Kanji vs scrambled Kanji task, we found significant activation in the left inferior occipital gyrus. However, we found no activation in earlier visual cortices, including the primary visual cortex, indicating that the scrambled Kanji served as an effective control stimulus for this task. In the Kanji vs blank screen task, much more areas, including earlier visual cortices, were activated. The activation that we found in the Kanji vs scrambled Kanji task was compatible with the results of previous studies of English letter strings by other groups, suggesting that the left inferior occipital gyrus plays an essential role in orthographic processing common to these two different writing systems.

Arousal↗

Couplings of character and of chirality in the origin of the genetic system.

Data from the literature and new data presented here suggest that the genetic system (coding and protein synthesis) is based on relationships of character and structure between amino acids and nucleic acids. Character relationships seem to be anticodonic and structurally the greatest preferences are seen between the heteropair, L-amino acids and D-ribose nucleic acids. However, living systems using the other heteropair must have been equally likely. Homopairing (L-L and D-D) in living systems seems unlikely. Awareness of the heterocoupling of steric forms narrows somewhat the problem of understanding the origin of chirality.

Adenosine↗

An experimental approach to evaluation of the relative hydrophobic character of biological liquids and tissues.

Total proteins extractable from a number of rat tissues were partitioned in the aqueous Ficoll-400-Dextran-70 biphasic system. The partition coefficient of the total proteins extractable from a given tissue, K, was shown to be a tissue-specific constant. The free energy of transfer of a CH2 group from water to the aqueous solution of a given protein extract, delta(delta gCH2), was calculated from the corresponding K-value using the correlation relationship between K and delta(delta gCH2) reported earlier. It is suggested to use the parameter delta(delta gCH2)-value as a measure of the relative hydrophobic character of biological fluids and tissues. The difference between the delta(delta gCH2)-values for blood plasma medium and for a given tissue medium is used to quantify the difference in the relative hydrophobic character of the biological tissues and fluids under study. The possibilities to use the above characteristics in drug research are considered.

Animals↗