Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “AEROMONAS”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 595 records · Page 33Linked to original sources
PubMed1993

Incidence of Aeromonas species in diarrhoeic stool in University College Hospital Ibadan, Nigeria.

Between February and July 1989, stool samples from 100 diarrhoeic patients were screened for Aeromonas species. For isolation, alkaline peptone water was used for enrichment and xylose desoxycholate citrate agar as differential and selective medium. Only one sample (1%) yielded Aeromonas hydrophila having come from a 2-month old baby. No other enteric pathogens were isolated from the positive stool sample, a strong indication that A. hydrophila was responsible for the diarrhoea in the baby. Of nine antimicrobial agents used the lone A. hydrophila isolate was resistant only to ampicillin.

Adolescent↗
PubMed1992

A comparison of total and specific immunoglobulin levels in healthy Atlantic salmon (Salmo salar L.) and in salmon naturally infected with Aeromonas salmonicida subsp. achromogenes.

Healthy Atlantic salmon and salmon with a history of chronic natural Aeromonas salmonicida subsp. achromogenes infection were compared with respect to total serum protein and the concentration and specificity of serum immunoglobulin. The immunoglobulin level was measured using competitive ELISA and the specific antibody activity against Aeromonas salmonicida subsp. achromogenes was measured using double sandwich ELISA. Significant elevation of serum protein and immunoglobulin concentration was observed in the infected salmon compared with the healthy fish. This was accompanied by weak anti-A. salmonicida activity in the infected fish which seemed to contribute to the raised immunoglobulin level to only a limited degree.

Aeromonas↗
PubMed1990

Distribution and characteristics of Aeromonas in food and drinking water in Denmark.

A total of 970 Danish commercial foods and drinking water samples were examined for the presence of motile Aeromonas spp. With a detection limit of 10(2)/g the frequent prevalence in raw foods was confirmed. Aeromonas occurred in 7% of 779 samples of prepared foods; most frequently in whipped cream from ice cream parlors (28%) and mayonnaise salads (10%) with numbers occasionally exceeding 10(5)/g. The prevalence in drinking water was 28% with a detection limit of 1/100 ml. A hydrophila was the dominating species in both food and water. Hemolysin production was demonstrated in 37% of the 51 isolates tested with 10% having high titers.

Aeromonas↗
PubMed1991

Incidence of Aeromonas and Listeria spp. in red meat and milk samples in Brisbane, Australia.

A total of 150 samples, 50 each of beef, lamb and pork from 10 local retail stores in Brisbane metropolitan area as well as 150 raw bovine bulk milk tank samples obtained from Queensland United Foods (QUF), were examined for the presence of Aeromonas and Listeria spp. over a period of 1 year. Different sets of enrichment and plating media were used to recover the organisms with subsequent identification using conventional biochemical and serotyping techniques. A total of 509 isolates consisting of Aeromonas spp. (350) and Listeria spp. (159) were obtained from 60%, 58%, 74%, 26.6%, 34%, 40%, 30%, 2.6% of samples of beef, Lamb, pork and milk respectively. Motile aeromonads (A. hydrophila, A. sobria, A. caviae) and Listeria innocua were isolated from all kinds of samples whereas L. monocytogenes was only isolated from flesh food. A. hydrophila contributed the largest percentage of the motile aeromonads (60%). The majority of L. monocytogenes cultures were serotype 4.

Aeromonas↗
PubMed1995

Comparison of seven selective media for the isolation of mesophilic Aeromonas species in fish and meat.

Seven selective agar media and two enrichment broths were evaluated for their suitability for the isolation of mesophilic Aeromonas species from meat, fish, and shellfish samples. In a first trial, aeromonads were inoculated in fish and meat samples and reisolated using all selected media. For qualitative isolation, enrichment in alkaline peptone water (pH 8.7 +/- 0.1) at 28 degrees C and subsequent plating onto sheep blood agar supplemented with 30 mg/L ampicillin (ASBA 30) and bile salts-irgasan-brilliant green agar (BIBG) at 35 degrees C led to the best results. For quantitative assays, direct plating on the same agar media is recommended. In a second trial, 829 meat, fish, and shellfish samples were investigated with the same methods. The results show that BIBG is the most selective medium and that presumptive identification of aeromonads on ASBA 30 is very easy. Finally, we could confirm the opinion of other workers that optimal recovery of mesophilic Aeromonas spp. requires the use of more than one agar medium.

Aeromonas↗
PubMed1996

Incidence and toxigenicity of Aeromonas hydrophila in seafood.

Three selective media, Oxoid Aeromonas agar (OA), blood ampicillin agar (BA) and starch ampicillin agar (SA) were used to evaluate the presence of Aeromonas hydrophila in 66 samples of oyster, shrimp, fish and surimi products. Oyster had the highest incidence, with 50% positive, whilst no A. hydrophila was found in the surimi. Of the three selective media, BA displayed the highest recovery rate of A. hydrophila from seafood. Forty-eight isolates from this survey were tested for their capability to produce hemolysin and cytotoxin. Hemolysin was produced by 79.2% of the isolates and cytotoxin was produced by 91.7% of the isolates in brain heart infusion broth. One of the toxin-producing isolates from oyster, strain 8-169, was further tested for growth and toxin production in oyster, shrimp and fish at various temperatures. This particular isolate grew best and had highest toxin production in oyster. Hemolysin and cytotoxin were produced earlier at 28 degrees C than at 37 degrees C, and titers of hemolysin were also higher at 28 degrees C. At 5 degrees C, it was able to grow and produce hemolysin in oyster.

Aeromonas hydrophila↗
PubMed1984

The use of a biotyping system to investigate an unusual clustering bacteraemias caused by Aeromonas species.

Aeromonas spp. were isolated from blood cultures taken from four clinically bacteraemic patients over an 18 day period on four separate wards. A common source was suspected and extensive environmental sampling revealed two more ward isolates of Aeromonas spp. A biotyping system was employed which distinguished the strains from each other and indicated that a common source was unlikely. This coincidental clustering occurred in the autumn, a period when isolates from water and faeces are normally at a peak. All patients were debilitated and it is postulated that their own gastrointestinal tracts acted as the most likely route for their bacteraemias.

Aeromonas↗
PubMed1995

Non-enteritic aeromonas infections in hospitalized patients.

Aeromonas species were isolated from specimens other than faeces from 59 hospital inpatients over a 15 year period. Of the isolates, 79.7% were regarded as clinically significant, with skin and soft tissues and blood cultures as the commonest sites of infection. Of the isolates, 52.5% were hospital-acquired, and 55.9% of patients had serious underlying disease. Community-acquired infections in previously healthy individuals accounted for only 13.6% of isolates. However unlike many other opportunistic infections, aeromonas infection was not closely associated with prior antibiotic therapy, nor was there a significant increase in the frequency of infection over the study period.

Adolescent↗
PubMed1989

Aeromonas hydrophila infection complicating digital replantation and revascularization.

Two cases of thumb replantation and one of finger revascularization complicated by Aeromonas hydrophila infection are reported. Two digits were lost because of infection in soft tissue and osteomyelitis. One thumb had extensive necrosis. In all cases the infection was difficult to eradicate, probably because of ischemia. All three patients sustained their injuries while cutting meat or fish. These infection sources have not been previously reported. Note is made of Aeromonas hydrophila in medicinal leeches that are used in microvascular surgery and the potential for iatrogenic infection.

Accidents, Occupational↗
PubMed1989

Aeromonas hydrophila upper extremity infection.

A severe soft tissue infection of the upper extremity caused by Aeromonas hydrophila followed a water skiing injury in which a tow rope caused degloving of a portion of the skin and severe contusion to underlying muscle. Infection was established within 36 hours of the injury, accompanied by fever, leukocytosis, and a foul odor. Rapid clinical improvement occurred following radical debridement of all nonviable tissue and antibiotic therapy. In cases involving water contamination, Aeromonas hydrophila infection should be suspected with the onset of a rapidly developing infection with a febrile response. Rapid surgical intervention and treatment with an aminoglycoside or a third-generation cephalosporin antibiotic is the treatment of choice.

Adult↗
PubMed1990

Isolation and characterization of bacteriophage PM3 from Aeromonas hydrophila the bacterial receptor for which is the monopolar flagellum.

PM3 is an Aeromonas-specific bacteriophage which was isolated and characterized on A. hydrophila strain TF7. Spontaneous mutants resistant to PM3 were non-motile having lost their characteristic monopolar flagellum. In addition, purified flagella inactivated PM3. PM3 is the first filamentous bacteriophage isolated on Aeromonas, the adsorption site for which is the monopolar flagellum.

Aeromonas↗
PubMed1991

Survival of the fish pathogen Aeromonas salmonicida in seawater.

Survival of Aeromonas salmonicida in natural (non-sterile) seawater, as determined from colony counts on marine agar, was found to be influenced by the presence of potentially inhibitory organisms, i.e., Acinetobacter, Aeromonas hydrophila, Chromobacterium, Escherichia coli, Flavobacterium and Pseudomonas, and their metabolites. Yet, samples, thought to be devoid of culturable A. salmonicida, were found to contain cells, which were filterable through 0.22 and 0.45 microns Millipore Millex porosity filters, and were recoverable on a specialised medium for L-forms, i.e. L-F medium.

Aeromonas↗
PubMed1991

Cloning and characterization of an extracellular temperature-labile serine protease gene from Aeromonas hydrophila.

Aeromonas virulence is thought to depend on multigenic functions. The gene for an extracellular protease from Aeromonas hydrophila SO2/2 was cloned in Escherichia coli C600-1 by using pIJ860, bifunctional plasmid, as a vector. The gene encodes for a temperature-labile serine protease (P2) with a molecular mass of approx. 68 kDa which is highly inhibited by PMSF. The gene was expressed in Streptomyces lividans 1326 by transforming protoplasts with the original clone pPA2. We were also able to transfer and express the prt P2 gene in Pseudomonas putida by mating experiments. The protein P2 was secreted into the periplasms of both P. putida and E. coli C600-1 being identical in properties to one of the proteases secreted into the culture supernatant by A. hydrophila SO2/2.

Aeromonas↗
PubMed1992

Mechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila.

In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila. Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE2 levels in CHO cells, suggesting a cholera toxin-like mechanism of action for Aeromonas cytotonic enterotoxins.

Aeromonas hydrophila↗
PubMed1994

Cloning and expression of putative cytotonic enterotoxin-encoding genes from Aeromonas hydrophila.

A genomic library from a diarrheal isolate, SSU, of Aeromonas hydrophila was constructed in a cosmid vector, pHC79, and in bacteriophage lambda EMBL3. Cell lysates from various Escherichia coli clones containing the recombinant cosmid were examined for their ability to elongate Chinese hamster ovary (CHO) cells, which is a typical enterotoxic response. Based on restriction analysis, a 4.0-kb SalI DNA fragment from one of the clones that exhibited enterotoxic activity was subcloned into a bacteriophage T7 RNA polymerase/promoter hyperexpression system. The cell lysate from this E. coli [pSL24] clone caused CHO cells to elongate and revealed the presence of a major 35-kDa polypeptide by [35S]methionine labeling and sodium dodecyl sulfate (SDS)-polyacrylamide-gel electrophoresis (PAGE). The toxin was biologically heat labile, losing all activity within 20 min at 56 degrees C. In addition, another enterotoxin-producing clone, E. coli[pSBS32], was isolated from cosmid and lambda bacteriophage libraries. We localized this heat-stable (56 degrees C/20 min) enterotoxin to a 4.8-kb SalI-BamHI fragment. Both enterotoxins caused elevation of cyclic adenosine monophosphate (cAMP) in CHO cells. The DNA fragments encoding these enterotoxins did not hybridize with each other. However, a 4.8-kb SalI-BamHI DNA fragment encoding a heat-stable enterotoxin hybridized to a 3.5-kb BamHI DNA fragment of a plasmid, pHPC100, that contained a cytotonic enterotoxin-encoding gene isolated from A. trota. Our data suggest Aeromonas species produce different structural types of cytotonic enterotoxins that are functionally similar.

Aeromonas hydrophila↗
PubMed1988

Suppurative thrombophlebitis due to Aeromonas.

A patient developed lethal suppurative thrombophlebitis and adjacent soft-tissue infection caused by Aeromonas. Potential risk factors included corticosteroid therapy and the use of warm tap water compresses at the site of intravenous catheter-related phlebitis. This case demonstrates the rapidly invasive characteristics of Aeromonas and the need for early surgical intervention in suppurative thrombophlebitis.

Aeromonas↗
PubMed1992

Purification and characterization of Aeromonas sobria Ae24 pili: a possible new colonization factor.

Pili of Aeromonas sobria Ae24 were purified and characterized. The molecular mass of the pilin was estimated to be about 19 kDa by SDS-PAGE. The Ae24 pili were electrophoretically distinguishable from previously reported Aeromonas hydrophila Ae6 W pili and A. sobria Ae1 pili, although all three had indistinguishable morphology and shared a high degree of homology in the N-terminal amino acid sequences. Strain Ae24 and its purified pili adhered to rabbit intestine and agglutinated human and rabbit erythrocytes. Hemagglutination was inhibited by D-galactose and D-mannose, but not by L-fucose. Organisms pretreated with Fab fraction of the antipilus antibody failed to adhere to the intestine. Organisms did not adhere to intestine pretreated with the purified pili. These findings suggest that the pili are a colonization factor of A. sobria Ae24 for the rabbit intestine, and that the receptor is galactose- and mannose-containing structure.

Aeromonas↗
PubMed2006

Comparative study of cytotoxicity of Aeromonas spp. on four different cell lines.

In vitro cytotoxicity is an important virulence property of motile mesophilic Aeromonas species. Cell-free supernatant prepared from 55 Aeromonas isolates including one A. hydrophila type strain (MTCC 646) were examined for their cytotoxic potential on four different cell lines (Vero, BHK-21, MDBK, B 95a). Results of the study revealed cytotoxic potential in 92.72% of the isolates. Analysis of data exposed significant variation among isolates in respect of their cytotoxicity. Vero cells proved to be most sensitive to aeromonal toxins and B 95a cells showed significantly (P<0.01) lower response compared to other cell lines. Sensitivities of BHK-21 and MDBK cell lines were in between Vero and B 95a.

Aeromonas↗