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Milk yield, somatic cell counts, and risk of removal from the herd for dairy cows after covered teat canal injury.

The objective of this study was to evaluate milk yield, somatic cell count (SCC), and risk of removal of a cow from the herd after covered teat injury. Teat injuries were diagnosed and treated by using endoscopy. After treatment, teats were rested for 3 x 3 d. Eighty-one cows referred to the Veterinary Clinic Babenhausen were used for this study. Each cow was matched to three herdmates by breed, age, and calving date. Data on milk yield and SCC were available from the records of the Bavarian milk control board. Test day milk yields and lactational milk yields were equal for cows with covered teat injury and herdmates in the lactation when the injury was diagnosed and in the subsequent lactation. Calving interval in the year the injury was diagnosed and the time cows lived in the herd were also equal. However, covered teat injuries significantly increased test day SCC by 128,000 cells/ml of milk. These injuries also significantly increased the odds of subclinical mastitis (SCC > 100,000 on test day) and the odds of violating European milk shipping regulations (SCC > 400,000 on test day). Because increased SCC was significantly associated with decreased milk yield, cows may not have fully utilized their milk yield capacity after covered teat injury.

Animals↗

Monitoring daily milk yields with a recursive test day repeatability model (Kalman filter).

Mixed model methodology and recursive estimation techniques (Kalman filter) were combined to detect significant changes in the performance level of both individual cows and an entire herd. Yields were predicted for the next time of recording using all data available up to that time. Predicted yields were compared with actual measurements. If the error of prediction, or innovation, exceeded +/- 2 times its standard deviation, the observation was considered to be significantly different from the former yield level. The data comprised 30,199 records for 135 cows and 366 d. Effects fitted in the model were test day, breed, and lactation class as fixed effects and cow within lactation number as a random effect. A lactation curve was fitted within lactation class. Of the observed milk yields, 9.2% deviated significantly from the expected value in a negative direction. None of the innovation of the fixed-day effect exceeded the threshold of two standard deviations. Compared with the results of rolling average, which were calculated as the average of a 10-d period, over 20% of the observations of the daily milk yield were classified differently by the two methods. The mixed-model method for recursive estimation takes better account of environmental and lactational effects influencing daily milk yields as the rolling average. The mixed-model recursive estimation method was applicable for the detection of suspicious (i.e., outside a specified prediction interval) observations of individual cows at the time of the actual recording.

Animals↗

Effects of low, normal, and high temperatures on slaughter yield of broilers from lines selected for high weight gain, favorable feed conversion, and high or low fat content.

Male and female broiler chicks from five different broiler crosses (WI = Israeli chicks selected for increased body weight; LF and HF = Israeli chicks selected for low and high abdominal fat, respectively; FC and WN = Dutch chicks selected for improved feed conversion and increased body weight, respectively) were raised at low, normal, or high temperature. Slaughter yield, amount of breast meat, and abdominal fat were determined at 6 wk of age in all groups and at a body weight of 2,360 g for males and 1,965 g for females in the low- and normal-temperature groups, and at 8 wk in the high-temperature groups. Temperature, genotype, and sex influenced both absolute and relative weights of carcass, breast meat, and abdominal fat. Temperature had a negative effect on breast meat yield. Males were affected more by high temperature than females. A significant interaction between temperature and sex for breast meat yield was caused by a low yield for males at the high temperature. A similar interaction for proportion of abdominal fat was caused by a high fat content in males reared at the high temperature. Slaughter yield and especially yield of breast meat were highest in FC chickens in all comparisons.

Abdomen↗

Appropriateness of indication and diagnostic yield of colonoscopy: first report based on the 2000 guidelines of the American Society for Gastrointestinal Endoscopy.

AIM: To assess the appropriateness of referrals and to determine the diagnostic yield of colonoscopy according to the 2000 guidelines of the American Society for Gastrointestinal Endoscopy (ASGE). METHODS: A total of 736 consecutive patients (415 males, 321 females; mean age 43.6+/-16.6 years) undergoing colonoscopy during October 2001-March 2002 were prospectively enrolled in the study. The 2000 ASGE guidelines were used to assess the appropriateness of the indications for the procedure. Diagnostic yield was defined as the ratio between significant findings detected on colonoscopy and the total number of procedures performed for that indication. RESULTS: The large majority (64%) of patients had colonoscopy for an indication that was considered "generally indicated", it was "generally not indicated" for 20%, and it was "not listed" for 16% in the guidelines. The diagnostic yield of colonoscopy was highest for the "generally indicated" (38%) followed by "not listed" (13%) and "generally not indicated" (5%) categories. In the multivariable analysis, the diagnostic yield was independently associated with the appropriateness of indication that was "generally indicated" (odds ratio=12.3) and referrals by gastroenterologist (odds ratio =1.9). CONCLUSION: There is a high likelihood of inappropriate referrals for colonoscopy in an open-access endoscopy system. The diagnostic yield of the procedure is dependent on the appropriateness of indication and referring physician's specialty. Certain indications "not listed" in the guidelines have an intermediate diagnostic yield and further studies are required to evaluate whether they should be included in future revisions of the ASGE guidelines.

Adolescent↗

Yield of DNA strand breaks after base oxidation of plasmid DNA.

We have irradiated aerobic aqueous solutions of plasmid DNA with 137Cs gamma rays in the presence of inorganic radical scavengers including nitrite, iodide, azide, thiocyanate and bromide. These scavengers react with the strongly oxidizing hydroxyl radical (*OH) to produce less powerful oxidants. Of these scavengers, only thiocyanate and bromide result in the formation of oxidizing species [(SCN)2*- and Br2*-, respectively] which are capable of reacting with the bases in DNA. The oxidized bases were detected after incubation of the irradiated plasmid with the two E. coli DNA base excision repair endonucleases, formamidopyrimidine-DNA N-glycosylase and endonuclease III. Depending on the experimental conditions, the intermediate base radicals may ultimately form stable oxidized bases in very high yields (within an order of magnitude of the *OH yield), and possibly also single-strand breaks (SSBs) in much lower yield (between 0.1 and 1% of the total yield of base damage). By competing for (SCN)2*- with an additional species (nitrite), it was possible to estimate the second-order rate constant for the reaction of (SCN)2*- with DNA as 1.6 x 10(4) dm3 mol(-1) s(-1), and also to demonstrate a correlation between the large yield of damaged bases and the much smaller increase in the yield of SSBs over background levels due to *OH. The efficiency of transfer of damage from oxidized base to sugar is estimated as about 0.5% or 5%, depending on whether purine or pyrimidine base radicals are responsible for the base to sugar damage transfer.

DNA Damage↗

Yield of gastric lavage and bronchial wash in pulmonary tuberculosis.

OBJECTIVE: To determine the yield of acid-fast bacilli (AFB) in gastric lavage and bronchial washing in adult patients clinically and radiologically suspected of pulmonary tuberculosis but who cannot produce sputum. METHODS: Selected adult patients were admitted to the ward; gastric lavage was done for 3 consecutive days after an overnight fast followed by bronchial wash. Specimens were immediately sent to laboratory for AFB direct smear and culture. RESULTS: The yield of AFB in gastric lavage on direct smear was 16/20 (80%) and 12/20 (60%) in the first and second samples, respectively. When combined, 18/20 (90%) were direct smear positive, while the third sample did not increase the yield. The yield of AFB culture in gastric lavage was 6/20 (30%) in both the first and second samples, while the combined results of the first and second samples were 8/20 (40%). The third sample did not increase the yield. In bronchial wash, AFB direct smear was positive in 18/20 (90%), while culture was positive in 14/20 (70%). CONCLUSION: Gastric lavage and bronchial washing are useful methods for the diagnosis of pulmonary tuberculosis in patients who cannot produce sputum. Two gastric lavage specimens are adequate. On comparison, bronchial wash is superior to gastric lavage in culture, but their yield on direct smear is equal.

Adolescent↗

The positive yield of imaging studies in the evaluation of men with newly diagnosed prostate cancer: a population based analysis.

PURPOSE: We determine the positive yield of imaging studies performed on men with newly diagnosed prostate cancer. MATERIALS AND METHODS: A prospective, population based survey was conducted on 3,690 men with prostate cancer diagnosed between October 1, 1994 and October 31, 1995. Cases were identified by the rapid case ascertainment systems used in 6 geographic regions participating in the Surveillance, Epidemiology and End Results Program. Based on information captured in primary medical record reviews we estimated the positive yield of bone scans, computerized tomography (CT) and magnetic resonance imaging. RESULTS: The positive yield of bone scan and CT was less than 5% and 12%, respectively, for all men with prostate specific antigen (PSA) 4 to 20 ng./ml., and less than 2% and 9%, respectively, for those who also had a Gleason score of 6 or less. Only men with PSA greater than 50 ng./ml. and those with Gleason scores 8 to 10 and PSA greater than 20 ng./ml. had positive yields greater than 10% and 20% for bone scan and CT, respectively. CONCLUSIONS: Imaging studies designed to identify metastases and/or extracapsular extension in men with newly diagnosed prostate cancer frequently have a low positive yield. Wide variations exist in the use of imaging studies and are associated with tumor factors, such as Gleason score and serum PSA, and nontumor factors, such as state of residence. More extensive cost-effectiveness analyses are needed to define appropriate guidelines for ordering imaging studies to optimize the positive yield among men with newly diagnosed prostate cancer.

Aged↗

[Effect of plastic film mulching on crop yield and nitrogen efficiency in semiarid areas].

The effect of plastic film mulching, water storage in soil profile before sowing, and nitrogen fertilization on crop yield and nitrogen efficiency was examined in this paper. The study site was on the cultivated lossial soil in semiarid areas with 415 mm of annual rainfall and the test crop was spring wheat, Triticum aestivum. In order to study the effect of plastic film mulching, 4 levels of mulching were designed, including mulching of 0, 30 and 60 days after sowing and mulching over the whole growing period. The results showed that increase of soil water storage, plastic film mulching and nitrogen fertilization increased crop yield significantly(alpha < 0.01), and their effect followed in the order of nitrogen fertilization > increase of water storage > plastic film mulching. The effect of mulching on crop yield varied with water storage, nitrogen fertilization and mulching periods. When the water storage was low, there was no significant difference in crop yield between mulching and no mulching, although mulching increased crop yield slightly, and the nitrogen efficiency was higher for no mulching and mulching 30 days. When the water storage was high, the difference between the yield of mulching 60 days and no mulching was significant, but no difference in nitrogen efficiency was found for mulching 30 days, 60 days and over whole growing period. It was suggested that mulching over whole growing period was of less significance in practice.

Crops, Agricultural↗

Buccal cell DNA yield, quality, and collection costs: comparison of methods for large-scale studies.

There is considerable interest in noninvasive and cost-effective methods for obtaining DNA in large-scale studies. In this randomized crossover study of 22 participants, we compared the DNA yield, quality, and associated costs of buccal cell DNA collected using cytobrushes (three brushes per collection) and swish (i.e., mouthwash) in self-administered procedures. There was a nonstatistically significant higher yield from the mouthwash compared with cytobrush collections (15.8 microg versus 12.0 microg, respectively; P = 0.53). PCR reactions that required short (0.3 kb) or intermediate (1.1 kb) DNA fragments were 100% successful for DNA from brush and mouthwash, whereas PCRs for reactions that required long fragments (7.8 kb) failed for all of the participants from cytobrush DNA and were 81% successful for DNA from the mouthwash source. The brush collections provided sufficient DNA for an estimated 150-225 PCR reactions requiring short and intermediate DNA fragments. The estimated per person costs for buccal brush DNA collections in large studies were less then half (8.50 dollars) those for the mouthwash method (18 dollars). In addition, we tested whether cytobrush instructions to rub cheeks before collection or collect cells only in the morning increased DNA yield and whether repeat brushings of the same cheek reduced DNA yield. These variations resulted in no significant differences in DNA yields. We conclude that the collection of DNA with cytobrushes using simple instructions is cost effective in large-scale studies, and yields sufficient quantity and quality of DNA for genotyping.

Cost-Benefit Analysis↗

[Regulation of fertilizer application on yield and root growth of spring wheat-faba bean intercropping system].

The effects of N and P fertilizer application on the multiple population yield and root growth of spring wheat (Triticum aestivum)--spring faba bean(Vicia sativa)intercropping system were studied by two field experiments with 0, 150 and 225 kgN.hm-2, three pot and pool experiments with 2 N applications (0, 100 mg N.kg-1 soil) and 3 P applications (0, 100 and 200 mg P.kg-1 soil). The results showed that this intercropping system had a significant advantage of yield and beneficial effects. Compared to Po supply, P fertilization increased the seed yield by 48.39% for intercropped spring wheat and 16.69% for intercropped faba bean in field experiment. Furthermore, the total seed yield of both crops was increased by 20.07% to 43.14% in pool culture, and the grain yield of intercropped faba bean was increased by 58.46% to 78.78%. In the intercropping system, the growth peak of root density of both crops was appeared alternately, the peak of wheat root growth being earlier than that of faba bean. The maximum root weight of intercropped wheat was in its early heading periods, whereas that of faba bean appeared at its maturity periods, which reduced the competition for water and nutrients between the crops, and resulted in yield increase. The weight, length and surface area of wheat roots in P supply were increased by 54.33%, 48.88% and 47.00%, and in N supply, they were increased by 15.25%, 11.61% and 11.46%, respectively. About 57.61% of wheat root weight and 69.20% of faba bean root weight were distributed at 0-30 cm soil depths in the treatments of receiving P fertilizer at various rates, which indicated that P supply increased root weight and length of both crops in the intercropping system.

Crops, Agricultural↗

[Effect of seedling stage shading on cotton yield and its quality formation].

The effect of shading during symbiotic stage of inter-cropped cotton and wheat on cotton yield and its quality formation was examined by simulated shading at the seedling stage of cotton. The results showed that the effect of shading on the cotton boll formation depended upon the position of fruiting branch and fruiting bud. For the bolls at low(1-3 fruiting branches) and middle(4-6 fruiting branches) parts and the inside bolls(1-2 fruiting buds) on upper(7-9 fruiting branches) fruiting branch, shading favored boll formation, but for the outside bolls (> or = 3 fruiting buds), especially at the top fruiting branch position, shading was unfavorable to boll formation. The effect of shading on the weight of individual boll also depended upon the position of fruiting branch and fruiting bud, but shading did not affect the mean boll weight per plant. For seed cotton yield per plant, the inside boll yield of shaded low and middle fruiting branches was higher than the control, but that of the upper and top fruiting branches was just contrary, the yield of outside bolls of all branches was lower than the control. For the distribution of the inside and outside bolls, shaded cotton was 1:0.36, versus 1:0.58 for the control. For the yield distribution, shaded cotton was 1:0.42, versus 1:0.72 for the control. The effect of shading on the cotton fiber and seed quality was mainly restricted on the bolls of top fruiting branches and the outside bolls of upper fruiting branches. The results also showed that different cotton varieties were different in their shade-tolerance, according to comprehensive analysis of the yield and quality formation, and the shade-tolerance of variety Zh9418 was stronger than that of Zh19 and CAZ.

Cotton Fiber↗

Impact of strobilurins on physiology and yield formation of wheat.

Strobilurin fungicides have a broad spectrum activity against all major foliar pathogens of wheat. In addition to this extraordinary fungicidal activity side-effects have been reported which result in higher yields of cereals, e.g. the reduction of respiration, delayed leaf senescence, activation of nitrogen metabolism as well as increased tolerance against abiotic stress factors. In the vegetation period 2000/2001 field trials were carried out at three sites in North Rhine-Westphalia to study the effects of three strobilurin fungicides on the yield formation of six winter wheat varieties. The strobilurins were applied two times as the commercial products Stratego (trifloxystrobin + propiconazole), Amistar/Pronto Plus (azoxystrobin/spiroxamine + tebuconazole) and Juwel Top (kresoxim-methyl + epoxiconazole + fenpropimorph. Fungicide-treated plants were kept disease-free by an initial azole-application in GS 31 in order to exclude disease effects on physiological parameters relevant to yield formation. Photosynthetic electron transport of strobilurin-treated wheat, was improved as early as at GS 65 compared to azole-treated plants. Differences often increased with growth stage and were closely related to a delay in leaf senescence. A higher photosynthetic activity of strobilurin-treated plants was confirmed by gas exchange and chlorophyll fluorescence measurements under field conditions. The yield benefit of wheat from strobilurin treatments varied from 2% to 9% depending on an improved photosynthetic capacity due to a higher and/or prolonged activity. Neither yield potential nor disease susceptibility of the cultivar had an effect on the height of the extra yield which, in contrast was modified by location and wheat genotype.

Acetates↗

[Interactive effect of irrigation and nitrogen application on physiological characteristics of flag leaf and grain yield of winter wheat in dry years].

The field studies indicated that in dry years, both irrigation and nitrogen application had a marked yield increment effect, but irrigation played a more important role. Irrigation at jointing and booting stages could induce a higher grain yield, but irrigation at jointing, booting and grain-filling stages had a less yield increment effect if nitrogen was applied as base fertilizer. Insufficient irrigation reduced the nitrogen efficiency and yield, which could be compensated to some extent by increasing the nitrogen application rate. Only a suitable combination of irrigation and nitrogen application could effectively coordinate the relationship between yield components and increase grain yield.

Chlorophyll↗

[Body condition and metabolic stability as the basis for high milk yield and undisturbed fertility in dairy cows--a contribution for deduction of reference values].

The target of this study was to describe the interactions between body condition and various descriptors of yield and fertility. It was aimed to identify an optimal conditional range to be used in herd management which combines high milk yield with acceptable fertility traits and general health. For this purpose, backfat thickness was measured by ultrasound at 46111 dairy cows on 78 different farms and was subsequently related to production variables. Negative energy balance is getting more intense and prolonged with increasing milk yield. However a conditional nadir below 10 mm leads to decreased milk production. To reach a high production level without an increasing incidence of health disorders, conditional nadir should not decline below 13 mm backfat thickness on herd average. Lower value only lead to negligibly higher milk yield but cause a distinctively higher risk of fertility problems and culling. High herd yields do not have to be at expense of reproduction performance and can be achieved without extreme body condition losses. An efficient herd management can offset depression in fertility, which commonly is combined with increasing milk yield. A standard curve for backfat thickness throughout lactation is suggested to be used in dairy herd management.

Adipose Tissue↗

[Effects of soil moisture on photosynthetic characteristics of different specialized end-uses winter wheat at their later growth stages and on their yields].

In this study, pot experiments with various cultivars of specialized end-uses winter wheat were carried out at Henan Agricultural University Research Station during 2001-2003. The results showed that as for cultivar Yumai 34, the values of its chlorophyll meter (SPAD), PSII activity (Fv/Fo) and maximal PSII light energy transformation efficiency (Fv/Fm) were the highest when soil moisture content was at 60% of field capacity (FC), but the photochemical quenching coefficient (qP), non-photochemical quenching coefficient (qN), apparent photosynthetic electron transport rate (ETR) and the ratio of PSII photochemical quantum yield (phi2) were all increased at 80% of FC. For high-yielding cultivar Yumai 49, its SPAD, qP, qN and ETR were higher at 80% of FC, but Fv/Fo, Fv/Fm and phi2 were less affected by FC. For cultivar Yumai 50, the values of its photosynthetic characteristics were the highest at 80% of FC, except that the qN value was the lowest. The yield and the relative content of grain protein of cultivar Yumai 34 were also increased at 60% of FC. Furthermore, there was a significant difference among grain yields. The relative contents of grain protein of cultivars Yumai 49 and 50 were improved at 40% of FC, while the highest yields were obtained at 80% of FC. There were significant differences in the grain yields of Yumai 49 and 50 among different soil moisture treatments.

Biomass↗

[Geographic distribution of winter wheat yield loss risk and its classification in North China].

Based on the theory of risk analysis and the nearest 50 years practical unit yield and meteorological data of North China winter wheat region, this paper discussed the assessment of winter wheat yield loss risk and its classification. The mean yield reduction rate of past years, variation coefficient of yield reduction rate in disaster years, different yield reduction rate and its occurrence probability, and disasters-resistance index were selected as the risk assessment indices, and the assessment showed that the distribution of these indices in the region had evident regional differentiation and certain continuity. The model of integrated risk index of North China winter wheat yield loss was established on the basis of the assessment, and the classification index of the risk was put forward. According to the classification result, North China winter wheat region was divided into three risk types, i.e., high risk type mainly including the Loess Plateau areas of Shanxi and Shaanxi Provinces and the parts of North China Plain, medium risk type including the northeastern part of North China Plain, south Henan Province and Taihang Moutain region, and low risk type including most parts of North China Plain and the central Shanxi plain.

Biomass↗

QTL for yield and its components responded to elevated CO2 in rice (Oryza sativa L.).

FACE (free air carbon dioxide enrichment) technology may provide a means by which the environment around growing plants can be modified to realistically simulate the concentration of atmospheric CO2 in the future. The plant growth and its yield of plant species can be enhanced under FACE. Identification of genomic regions influencing the response of yield and its components to elevated CO2 will be useful for understanding the genetics of active response to changed CO2 environment and developing higher yield cultivars, which will be adapted to future enriched atmospheric CO2 environment. A mapping population of 65 indica (IR24) chromosome segment substitution lines (CSSLs) in japonica (Asominori) background and their parents were used to detect QTLs for yield and its components, e. g. number of fertile tillers per plant (FT), 1000-grain weight (TGW), number of grains per panicle (GP) and grain yield per plant (GY) under FACE (200 micromol CO2/mol above current levels) and current CO2 concentration (Ambient, about 370 micromol CO2/mol) in the field experiment. The results showed that, GY, GP and FT of two parents under FACE were significant greater than that under Ambient. The transgressive segregation of the four traits was observed in the CSSLs population under both FACE and Ambient. A total of 20 QTLs for the four traits were detected on chromosome 1, 2, 4, 6, 7, 9 and 12 with LOD (Log10-likelihood ratio) of QTLs ranging from 2.5 to 5.7. Three QTLs were detected under both FACE and Ambient. However,other QTLs were detected only under one level of CO2, which indicated that these loci were sensitive to CO2 concentration. Additionally, two QTLs qFT12 and qGP4 were found for the QTL x Environment (QE) interaction effects. It is suggested that there is a high possibility to improve the yield of rice under elevated CO2 through marker-assisted selection.

Biomass↗

Correlation of CD34+ cell yield in peripheral blood progenitor cell product with the pre-leukapheresis cell counts in peripheral blood.

INTRODUCTION: Accurate timing of the leukapheresis procedures is of paramount importance to get the best possible CD34+ cell yield in the minimum number of leukapheresis procedures. AIM: To find if pre-harvest CD34+ cell concentration in peripheral blood correlates with CD34+ cells in the product. MATERIAL AND METHODS: Sixty Leukapheresis procedures were performed for 25 patients (8 autologous and 17 allogeneic transplants) with hematological malignancies. Statistical analysis was performed to correlate the pre-harvest CD34+ cell count and the CD34+ cell yield. Volume processed during PBPC harvests was three times the blood volume. RESULTS: The best correlation was found between the leukapheresis product CD34+ cell count and the pre-harvest PB-CD34+ cell count (PCC=0.674) when compared with the other pre-harvest PB cell counts viz., WBC (PCC=0.229) and MNC (PCC=0.324). This correlation was better in the allogeneic harvest (PCC = 0.645) than the autologous harvest procedures (PCC = 0.348). Correlation analysis based on paired samples from the 60 leukapheresis procedures showed that when the pre-leukapheresis PB-CD34+ cell count was >20x10(3)/ul a yield of >1x10(6) CD34+ cells/Kg could be obtained in 95% of the cases and >2x10(6) CD34+ cells /Kg could be harvested in 68% of cases whereas when the pre-leukapheresis PB-CD34+ cell count was <5x10(3)/microl the yield was <1x10(6) CD34+ cells/Kg in 81% of the procedures. CONCLUSION: The yield of CD34+ cells in PBPC harvests depends on the pre-harvest CD34+ cell concentration and therefore it is more useful than the pre-harvest WBC or MNC counts for predicting the appropriate timing of the harvests and also to achieve the best possible yield of CD34+ cells.

Adolescent↗