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Acceptability of potatoes groun in West Texas.

This study indicated that methods used to prepare fresh potatoes were, in decreasing order: Baking, mashing, boiling, and frying. The two varieties of potatoes commonly grown in West Texas were acceptable and compared favorably with similar varieties available in a local supermarket. However, the potatoes grown in West Texas had lower specific gravity. The Norgold Russet variety was judged by a sensory panel to be more acceptable than the Viking. Baked potatoes had superior texture but poorer color than boiled potatoes. Storing potatoes under home-like conditions for twenty-eight days resulted in lower acceptability, weight loss, and lower specific gravity. Some Norgold Russet potatoes were rotten after twenty-eight days' storage. Specific gravity on the day of cooking was correlated with texture of cooked potatoes. Supermarket shoppers' comments suggest that research is needed on the acceptability of West Texas potatoes baked in microwave ovens.

Cooking↗

The influence of feeding diets containing different calcium-phosphorous ratios on the laying hen.

Six experiments were conducted to determine the influence of feeding diets containing different calcium-phosphorus (Ca:P)ratios at different times on the laying hen. Feeding a diet containing 0.75% P from 6 a.m. to 11 a.m. and a diet containing 1.50% P from 3 p.m. to 8 p.m. decreased serum calcium in three or four experiments and decreased specific gravity of eggs in four of four experiments, when compared to that of the birds fed the control diet which contained 0.75% P during both time periods. Specific gravity of eggs from hens fed a diet containing 0.75% P from 6 a.m. to 11 a.m. and a diet containing 0.33% P from 3 p.m. to 8 p.m. was increased in three of four experiments. Although the differences are not significant, with one exception, the trend in the experiments indicate that the level of P in the diet and time of intake can influence shell quality and serum calcium. The dietary treatments had no significant influence on feed consumption, egg production or egg weight during any of these experiments. It was postulated that the adverse or beneficial effect on specific gravity of eggs and serum calcium as a result of feeding diets containing various CaP ratios was due to the influence on calcium absorption and/or bone resorption.

Animals↗

Manure sampling procedures and nutrient estimation by the hydrometer method for gestation pigs.

Three manure agitation procedures were examined in this study (vertical mixing, horizontal mixing, and no mixing) to determine the efficacy of producing a representative manure sample. The total solids content for manure from gestation pigs was found to be well correlated with the total nitrogen (TN) and total phosphorus (TP) concentrations in the manure, with highly significant correlation coefficients of 0.988 and 0.994, respectively. Linear correlations were observed between the TN and TP contents and the manure specific gravity (correlation coefficients: 0.991 and 0.987, respectively). Therefore, it may be inferred that the nutrients in pig manure can be estimated with reasonable accuracy by measuring the liquid manure specific gravity. A rapid testing method for manure nutrient contents (TN and TP) using a soil hydrometer was also evaluated. The results showed that the estimating error increased from +/-10% to +/-30% with the decrease in TN (from 1000 to 100 ppm) and TP (from 700 to 50 ppm) concentrations in the manure. Data also showed that the hydrometer readings had to be taken within 10 s after mixing to avoid reading drift in specific gravity due to the settling of manure solids.

Animals↗

Determination of the percentage purity, active ingredients and other parameters in paracetamol and chloroquine syrups.

In this paper the percentage purity, active ingredients, specific gravity and the pH of paracetamol and chloroquine syrups were determined. For paracetamol the percentage purity ranged from 102.6-106.67, while the active ingredients (in mg/5 ml base) ranged from 123.2-128. The specific gravity ranged from 1.13-1.24 and the pH from 4.16-5.32. For the chloroquine the percentage purity ranged from 97-106.3, and the active ingredient 48.5-53.13, the specific gravity 1.17-1.27 and the pH 2.44-4.17. The results are discussed in relation to the purity, active ingredients and sources of the drugs. The drugs were coded. The paracetamol had a code of 1p-5p while the chloroquine was coded from 1c-5c.

Acetaminophen↗

Peritoneal fluid analysis in peripartum mares.

OBJECTIVE: To characterize, in mares, changes in peritoneal fluid that occurred within the first 7 days after routine foaling. DESIGN: Prospective observational trial. ANIMALS: 15 mares. PROCEDURE: Abdominocentesis was performed within 10 days before foaling and again 12 hours, 3 days, and 7 days after each horse foaled. Data recorded for each sample included total nucleated cell count, differential cell count, specific gravity, fibrinogen concentration, and total protein concentration. Smears of each sample were examined by a single clinical pathologist. RESULTS: There were not any significant differences over time in specific gravity, total protein concentration, fibrinogen concentration, total nucleated cell count, or number of small mononuclear cells. Mean numbers of neutrophils and large mononuclear cells in samples collected after foaling were significantly higher than mean numbers in samples collected before foaling. For 11 of 14 horses, all samples were characterized cytologically as transudates without cytologic abnormalities. CLINICAL IMPLICATIONS: Results of analysis of peritoneal fluid from peripartum mares suggest that nucleated cell count, protein concentration, and specific gravity of peritoneal fluid from mares that have recently foaled should be normal. Thus, peritoneal fluid abnormalities detected in mares within a week after foaling should usually be attributed to a systemic or gastrointestinal problem and not to the foaling process itself.

Animals↗

Seasonal variation in gene expression for loblolly pines (Pinus taeda) from different geographical regions.

In developing xylem, gene expression levels vary in different genotypes, at different stages of development, throughout a growing season, and in response to stresses. Commercially important characteristics such as wood-specific gravity are known to differ with seed source. For example, when grown on a common site, the specific gravity of Arkansas loblolly pine (Pinus taeda L.) trees is greater than that of Louisiana loblolly pine, and Texas loblolly pines have a greater specific gravity than loblolly pines from the Atlantic coast. A microarray analysis was performed to examine variation in gene expression among trees from different geographical sources when grown on a common site, and seasonal variation in gene expression in each seed source. We used microarrays containing 2171 expressed sequence tags (ESTs) with putative functions of interest, selected from several loblolly pine xylem partial cDNA libraries and a shoot tip library. Genes with significant variation in expression for each factor were identified. Many genes preferentially expressed in latewood compared with earlywood were for proteins involved in cell wall biosynthesis. Variation in gene expression among trees from the two seed sources in each growing season suggests that there may be more differences between South Arkansas trees and South Louisiana trees in latewood than in earlywood. Variation in gene expression among trees from different regions may reflect adaptation to different environments.

Gene Expression Profiling↗

Tamponade efficiency of perfluorohexyloctane and silicone oil solutions in a model eye chamber.

BACKGROUND/AIM: As no single tamponade agent yet fulfils all the requirements of a long term inferior tamponade, attempts have been made to mix tamponade materials. This study investigated perfluorohexyloctane (F(6)H(8)) and silicone oil solutions designed to take advantage of the high specific gravity and interfacial tension of the F(6)H(8) and the high viscosity of silicone oil. METHODS: Solutions of three different densities were examined (1.01, 1.03 and 1.06 g/cm(3)) inside transparent chambers made of surface modified poly(methylmethacrylate) (PMMA). RESULTS: Compared to F(6)H(8), the solutions had poorer contact with hydrophilic surface of the chambers. The higher the specific gravity of the solution, the better was the contact. The solution with a specific gravity 1.01 g/cm(3) is probably of no use clinically. CONCLUSION: The model eye chamber made of surface modified PMMA is an efficient way of screening and choosing solutions with promising physical properties. Solutions of silicone oil with F(6)H(8) in other proportions or with other semifluorinated alkanes may be of interest.

Chemical Phenomena↗

Brain edema and neurologic status with rapid infusion of 0.9% saline or 5% dextrose after head trauma.

We previously reported that intravenous (i.v.) administration of large volumes (0.2 ml/g) of either an isotonic dextrose-free solution or 5% dextrose solution given over 18 h after closed head trauma (CHT) in rats did not significantly affect neurological severity score or brain tissue specific gravity. However, it is possible that with more rapid administration, isotonic or 5% dextrose i.v. solutions may alter neurological outcome after CHT. Our study examined whether neurological severity score, brain tissue specific gravity and water content, and blood composition were significantly altered when 0.25 ml/g of either 0.9% saline or 5% dextrose was given i.v. over 0.5 h (rather than over 18 h) after CHT. Eight-four rats that survived ether anesthesia and CHT were randomly assigned to one of 11 experimental groups. Saline- and dextrose-treated rats were evaluated at 4 and 48 h after CHT and were compared to rats without CHT and to untreated rats at 4 and 48 h after CHT. There were no statistically significant differences in neurologic outcome and brain edema between the untreated and the saline-treated groups. However, 5% dextrose i.v. increased mortality (group 6 and 11, 50 and 0% survivors, respectively), decreased specific gravity in the noncontused hemisphere, and worsened neurologic outcome with and without CHT. Blood osmolality remained stable in comparison to the baseline value of 291.9 +/- 7.4 mOsm/kg (mean +/- SD).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Could conductivity be used as a parameter in urinalysis?

Conductivity is a non-linear function of electrolyte concentration in solutions and could be used as an indirect method. The aim of this study was to determine the feasibility of urine conductivity measurement, which is a simple, cheap and not time consuming method, in the evaluation of renal functions. Seventy-two patients whose primary diseases were not taken into consideration were enrolled in this study. First morning urine specimens were obtained from all the patients and evaluated for osmolality, conductivity, pH, specific gravity, protein, creatinine, urea, uric acid, glucose, sodium, potassium, chloride, inorganic phosphate and calcium levels. There was a significant positive relation between osmolality and creatinine, urea, sodium, potassium, chloride, inorganic phosphate, uric acid, conductivity and specific gravity. Conductivity was also determined to be positively related to osmolality (r: 0.390, p < 0.01), sodium (r: 0.326, p < 0.01) and uric acid (r: 0.345, p < 0.01). The patients were grouped as those with a urine osmolality of less or more than 290 m Osm/kg. H2O (group A and B respectively). Urine conductivity was 6.84 +/- 5.35 (0.16-23.2) mScm-1 in group A and 10.6 +/- 5.25 (0.12-192) mScm-1 in group B. The difference was statistically significant (p = 0.005). When the spectrum of conductivity values were evaluated separately in each group, 74% of the patients in group A and 33.9% of the patients in group B were determined to have a conductivity level of less than 7.338 mScm-1. In conclusion, urine conductivity has a positive relation with osmolality. In addition, while osmolality and specific gravity are effected by many non-electrolyte molecules, conductivity is only related to sodium and uric acid concentrations. In addition, urine osmolality and conductivity levels could be used to interpret the concentration of uncharged glucose molecules. These results suggest that conductivity could be used as a parameter in routine urinalysis.

Child↗

Case report: inverted contrast medium--urine level in the bladder on computed tomography.

Fluid-fluid levels within structures are caused by differences between the specific gravity of the fluids. This results in a characteristic appearance on computed tomography (CT), with urine which contains contrast medium and has a high specific gravity layering posteriorly in the dependent portion of the bladder, while lower specific gravity, non-opacified urine is found uppermost. We report a patient in whom the contrast medium-urine level was inverted because of sediment in the dependent part of the bladder.

Adult↗

Experimental closed head injury in rats: mechanical, pathophysiologic, and neurologic properties.

A model of closed head injury in rats was developed using a calibrated weight-drop device. The development of edema was studied in various brain regions (cerebral hemispheres, brain stem, cerebellum) using a linear specific gravity gradient column. Regional brain tissue density was measured within 1 min, at 15 and 60 min, 18 h, 4 and 10 days after injury to the left cerebral hemisphere, and was compared with values in sham-operated and control rats. Significant edema (i.e., reduced specific gravity) occurred only in the traumatized hemisphere and was maximal at 18 h. A neurologic severity score (NSS) was developed to evaluate the status of the rat after injury. Specific gravity was significantly correlated with NSS at 18 h after injury. The affected hemisphere displayed hemorrhagic lesions as early as one hour post head trauma (HT), which evolved into hemorrhagic necrosis at 18 h. A pathologic score, evaluated 18 h post HT based on size and severity of the lesion, was correlated with the NSS and evaluated for each rat at one hour and 18 h postimpact. This correlation was found to be highly significant. This model of brain injury may be useful in future studies on the effects of therapeutic agents.

Animals↗

Assessing hydration status.

PURPOSE: Understanding the importance of euhydration in humans in order to ensure good health in various situations, the purpose of this review is to examine the available techniques in assessing hydration status. RECENT FINDINGS: During the past 20 years, many indices have been developed to assess hydration levels accurately in humans. Changes in body weight, haematological and urine parameters, bioelectrical impedance, skinfold thickness, heart rate and blood pressure changes are among these indices. Plasma osmolality, urine osmolality and urine specific gravity are the most widely used markers of hydration. However, urine colour has also been used with reasonable accuracy when laboratory analysis is not available or when a quick estimate of hydration is necessary. Some data indicate that urine colour is as good indicator of hydration as plasma or urine osmolality or urine specific gravity. SUMMARY: Although there is no 'gold standard' for assessment of hydration status, it appears that changes in body weight, along with urine osmolality, specific gravity, conductivity and colour are among the most widely used indices. Furthermore, they provide reasonable results, especially when the analysis is based on the first morning urine sample.

Blood Chemical Analysis↗

0.45% saline and 5% dextrose in water, but not 0.9% saline or 5% dextrose in 0.9% saline, worsen brain edema two hours after closed head trauma in rats.

UNLABELLED: In this study, we examined the effect of four i.v. fluids (250 mL/kg) on blood glucose and osmolality and brain tissue specific gravity after closed head trauma (CHT) in rats. CHT was delivered at Time 0; blood was sampled at 60 min; fluid infusion began at 75 min and ended at 105 min. Blood was again sampled at 105 and 120 min, and brain tissue specific gravity was determined at 120 min. Five groups (one control and four fluid-treated groups) received CHT, and five other groups (one control and four fluid-treated) did not (n = 9 in each group). 0.45% saline (1/2 NS) and 5% dextrose in water (D5W) accentuated the decrease of brain tissue specific gravity (1.0366 +/- 0.0025 and 1.0368 +/- 0.0028, respectively; mean +/- SD) caused by CHT (1.0395 +/- 0.0036), but 5% dextrose in 0.9% saline (D5NS) and 0.9% saline (NS) did not (1.0431 +/- 0.0042 and 1.0389 +/- 0.0049, respectively). In addition, 1/2 NS decreased blood osmolality (248 +/- 6 mOsm/L), D5W increased blood glucose (1095 +/- 173 mg/dL), D5NS increased blood osmolality (350 +/- 5 mOsm/L) and glucose (1695 +/- 76 mg/dL), and NS caused no significant change. We conclude that administering hypoosmolar i.v. fluids after CHT causes a significant worsening of cerebral edema 2 h after CHT. IMPLICATIONS: We previously reported worse neurological outcome and/or mortality after closed head trauma in rats when 5% dextrose in water or 0.45% saline was given i.v. compared with 0.9% saline or 5% dextrose in 0.9% saline. The present results and our previous findings indicate that worsening of outcome after closed head trauma in rats may be caused more by edema formation than by hyperglycemia.

Analysis of Variance↗

Fluid loss and body composition of elite Kuwaiti soccer players during a soccer match.

AIM: The aim of this study was to assess the effect of the hot weather conditions of Kuwait on fluid loss (FL) and body composition of Kuwaiti soccer players. METHODS: During 5 preseason soccer games, 10 elite Kuwaiti soccer players participated in this study. The age and physical characteristics (mean+/-SD) of the subjects were: age 24+/-4.7 years; height 173.4+/-5.2 cm; mass 68.2+/-7 kg; and the percent body fat 12.3+/-3.7%, the mean temperature 45.4+/-2 degrees C, mean humidity was 23.6+/-4.2%. Measurements were taken at the beginning, half time, and the end of each game; FL was measured by body weight changes. Urine specific gravity was used to determine the state of hydration. Body composition was measured using Biodynamics Model 310e Body Composition Analyzer. RESULTS: There was a significant difference in body weight changes between 1st and 2nd half at P>0.05. The mean value of the FL at the end of the game was 3.1+/-1.4 L; indicating no significant difference in FL between 1st and 2nd half at P>0.05). The mean value urine specific gravity at the end game was 1.026+/-0.002; there was a significant difference between first, and second half for urine specific gravity (P>0.05). CONCLUSIONS: Results of this study indicated that the subjects did not consume enough fluid to offset FL. Players and coaches involved with activities in hot humid environments should pay closer attention to FL and body weight changes which occur during physical activity. Special care should always be taken to insure players consume extra water prior to each training session or game.

Adipose Tissue↗

Interrelationship between phosphorus, sodium, and chloride in the diet of laying hens.

Two experiments were conducted with commercial egg production type hens to determine the effects of dietary phosphorus, sodium, and chloride levels as related to levels of plasma calcium and phosphorus, blood acid-base balance, and production characteristics. Sodium and chloride were supplied to the diet as sodium bicarbonate and hydrochloric acid, respectively. In Experiment 1, five treatment groups were fed diets ad libitum containing .2, .6, 1.0, 1.4, and 1.8% total phosphorus. The remaining treatment group was fed a diet containing .4% total phosphorus from 1400 to 2030 hr and 1.4% total phosphorus from 600 to 1100 hr. In Experiment 2, a 3 X 4 factorial arrangement of treatments was used that consisted of three levels of total phosphorus (.2, .6, and 1.4%) and four levels of added sodium (0, .06, .22, and .45%) provided by sodium bicarbonate. Three other treatment groups were fed diets containing .36% supplemental chloride supplied by hydrochloric acid and either .2, .6, or 1.4% total phosphorus. Egg production was decreased in hens fed diets containing 1.8% total phosphorus. Egg specific gravity was decreased by both low and high phosphorus levels. Hens fed .4% total phosphorus in the afternoon produced eggs with higher specific gravity as compared with hens fed 1.4% total phosphorus during the entire experiment. At 1.4% dietary phosphorus, a significant increase in specific gravity was observed when .2 or .8% sodium bicarbonate was added to the diet. Increasing dietary phosphorus resulted in elevated plasma phosphorus and calcium concentration. Plasma phosphorus of hens fed .2 through 1.8% total phosphorus declined slowly from oviposition until 6 hr after oviposition and then reached a peak at 21 hr after oviposition.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

Effect of a prostacyclin analog OP-2507 on acute ischemic cerebral edema in cats.

We evaluated the inhibitory activity of a novel prostacyclin analog, OP-2507 (15-cis-(4-n-propylcyclohexyl)-16,17,18,19,20-pentanor-9-deo xy-6,9 alpha- nitriloprostaglandin F1 methyl ester) on the brain edema induced by occlusion of the middle cerebral artery in cats. Middle cerebral artery occlusion for 4h caused a decrease of regional cerebral blood flow. The specific gravity of the cerebral cortex measured 4h after the middle cerebral artery occlusion as an index of cerebral edema showed a significant reduction. Intravenous infusion of OP-2507 at infusion rates of 10 and 50 ng/kg per min was started 30 min before the middle cerebral artery occlusion and was continued for 4.5 h. While OP-2507 did not affect the blood pressure, heart rate and regional cerebral blood flow before and after the middle cerebral artery occlusion, the reduction of the specific gravity of cerebral cortex was significantly prevented by OP-2507 treatment at both doses. Prostacyclin prevented the reduction of the specific gravity only at the higher dose of 50 ng/kg per min. The present results indicate the potential usefulness of OP-2507 in acute ischemic cerebral disorders.

Animals↗

Response of White Leghorn hens to various dietary levels of Cassia obtusifolia and nutrient fortification as a means of alleviating depressed performance.

Two 28-day experiments with White Leghorn hens were conducted to determine the adverse effect level of ground Cassia obtusifolia seed (coffeeweed) in the diet and to examine the nutrient fortification required to improve depressed performance due to the presence of the seed. In both experiments, five individually caged hens were used for each of the four treatment replications. Treatments for Experiment 1 consisted of a control and six diets with ground C. obtusifolia seed substituted for a filler at levels of 2, 3, 4, 5, 6, and 7%. In Experiment 2, a series of nutritionally fortified (15 to 20% higher nutrient levels) and unfortified diets coupled with seed levels of 0, 4, 6, and 8% for a total of eight treatments was used. Average daily feed intake, hen-day egg production, egg weight, egg specific gravity, and body weight change were determined during each experiment. For Experiment 1, average daily feed consumption was measured every 24 h for the first 3 days. Egg production and feed consumption were significantly reduced by 2% seed in Experiment 1. Within the first 24 h, feed intake was decreased to 37% below that of controls by increasing to the highest seed level. Egg specific gravity dropped significantly below that of controls when hens were fed 5% seed. In Experiment 2, fortification improved feed consumption, hen-day egg production, body weight change, and egg specific gravity over that of birds fed unfortified diets with the same seed level. Finished diets contaminated with 2%, or more, C. obtusifolia seed can cause reduced performance in laying hens, but nutritionally fortifying these diets can help to alleviate depressed performance.

Animal Feed↗