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At least 577 records · Page 32Linked to original sources
PubMed1980

Use of the API 20E system to identify veterinary Enterobacteriaceae.

A total of 503 veterinary enteric bacterial pathogens obtained from state veterinary diagnostic laboratories were tested on API 20E strips to determine whether this rapid microidentification system could be utilized for veterinary clinical microbiology. The API 20E strip accurately identified 96% of the veterinary isolates and misidentified 3%. Identifications by the API system and the diagnostic laboratories were in agreement in 85% of the isolates, disagreement on 16% of the isolates, and 1% were not identified by the API strip. Differences in identification occurred primarily in distinguishing between Klebsiella and Enterobacter and between Enterobacter and Escherichia coli. These disagreements were most often due to incorrect identifications by the diagnostic laboratory rather than by the API system. Biotype differences between human and veterinary isolates were compared. Significant differences were noted in several biochemical reactions. The main differences observed for E. coli isolates were in ornithine decarboxylase production and melibiose fermentation. The largest differences for Salmonella occurred in arginine dihydrolase production, citrate utilization, and inositol fermentation, whereas for Klebsiella pneumoniae the main differences were noted in urease production and nitrate reduction. These biotype differences, however, did not affect the accurate identification of organisms on the API strip.

Animal Population Groups↗
PubMed1983

[Test strips for the rapid determination of alpha-amylase in urine. A cooperative study].

The value of a test-strip for the rapid determination of alpha-amylase in urine (Rapignost-Amylase) was tested at eleven different centres in four countries (Austria, Germany, Sweden, Switzerland) on a total of 1294 urine samples. Results were compared with those obtained by chromolytic and saccharogenic methods. The analytical efficiency of amylase determination in urine with the test-strip was 96%. There were 3.7% false-positive and 0.4% false-negative results. The test-strip readings were not influenced by erythrocytes or haemoglobin, glucose, protein, urobilinogen, barbiturates, benzodiazepines, codeine, amphetamine and ascorbic acid. Only bilirubin in very dark urine samples interfered with a correct reading of the test-strip.

Amylases↗