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[Anaerobic bacteria from microbial foci in acute odontogenic inflammatory diseases].

Mixed microbial associations from purulent foci of 30 patients with odontogenic inflammatory diseases were studied. 18 specimens of the clinical material were subjected to three-fold investigation. Bacteroids, propionic bacteria and pentococci predominated in the mixed cultures. Nonpathogenic Neisseria, streptococci of the mitis-salivarius group and epidermal staphylococci were isolated from the facultative anaerobic flora. The anaerobic species were the most sensitive to the antibiotics used in the treatment of the patients.

Acute Disease↗

[Nonclostridial anaerobic soft tissue infections].

Fourteen cases of nonclostridial anaerobic infections of soft tissues caused by peptococci, peptostreptococci, bacteroids or their combinations with E. coli, Proteus, and Staphylococcus aureus were studied. They are characterized by a rapid spread of serous-suppurative inflammation and the causative agents in loose fibrous connective tissue of subcutaneous adipose tissue (cellutitis), surface fascia (fasciitis), or skeletal muscles (myositis). Dissolution of intercellular structures of the connective tissue, extensive necroses, mild leukocyte reaction, and a wide peripheral zone of toxic edema are observed in the focus of inflammation. This is due to a high toxicity and enzymatic activity of the causative agents. Gas formation is observed when anaerobes are combined with E. coli.

Adult↗

[Saprophytic and opportunistic non spore-forming anaerobic microflora of the vagina (author's transl)].

A microbiological survey has been carried out on 179 healthy, child-bearing aged, non-pregnant women, with the aim to evaluate the incidence of anaerobic non-spore forming bacteria in the normal vaginal flora. This group of bacteria has been isolated in 50.3% of women, with a clear prevalence of "anaerobic Streptococci " and Bacteroides, followed by Fusobacterium and Veillonella. No Propionibacterium, Eubacterium or Bifidobacterium have been isolated. According to many Authors the non-spore forming anaerobes must be considered opportunistic bacteria, responsible of many infections of the female genital tract, especially when associated with other aerobic or facultative bacteria. Antibiograms have demonstrated a wide spectrum of activity of chloramphenicol and clindamycin; although not widely distributed, antibacterial activity have also shown metronidazole, penicillins, cephalosporins and lincomycin.

Adolescent↗

Ticarcillin disodium in anaerobic infections.

Twenty-five patients were treated with ticarcillin disodium, 18 of whom had anaerobic infections that included pleuropulmonary infections (seven), mandibular osteomyelitis (four), perirectal abscess (two), sepsis, primary site unknown (one), liver abscess (one), pelvic abscess (one), decubitus ulcer (one), and synergistic gangrene (one). Seven had no anaerobic infections. Three had anaerobic septicemia. Culture results included anaerobes: peptococci (ten), peptostreptococci (ten), Bacteroides fragilis (six), Bacteroides not fragilis (ten), eubacteria (three), fusobacteria (two), Clostridium (one), Veillonella (one), and acidaminococcus (one); aerobes: Proteus (three), Klebsiella (two), Escherichia coli (two), and streptococci (two). Six patients with mixed aerobic infections initially received gentamicin sulfate in addition. The serum levels were 110 +/- 20 microgram/ml one hour after intravenous infusion of 5 g of ticarcillin disodium. All anaerobic isolates were susceptible at less than or equal to 100 microgram/ml and 85% by less than or equal to 25 microgram/ml of ticarcillin. Sixteen patients responded well to ticarcillin and two failed to respond. Our study suggests that ticarcillin is useful in the treatment of anaerobic infections.

Anaerobiosis↗

Anaerobic and aerobic bacteriology of decubitus ulcers in children.

Forty-two children with decubitus ulcers were studied using aerobic and anaerobic techniques. Anaerobic bacteria were isolated in 21 (50%) of the patients, five times as the only isolates and 16 times mixed with aerobes. Aerobes only were present in 20 (48%) of the patients. There were a total of 83 isolates, 46 aerobes and 37 anaerobes, with an average of two species per specimen (1.1 aerobes and 0.9 anaerobes). The predominant anaerobic isolates were gram-positive cocci (17), Bacteroides fragilis (6), and Fusobacterium nucleatum (4). The most common aerobic isolates were Staphylococcus aureus (23), Group A beta hemolytic streptococci (6), Hemophilus influenzae (5), and Enterobacter agglomerans (5). The polymicrobial etiology of decubitus ulcers in hospitalized children is demonstrated.

Adolescent↗

[Nosocomial infections in present-day traumatological-orthopedic hospitals].

Nosocomial infection remains a significant problem in modern traumatology and orthopedics. Staphylococcus prevails in its etiological pattern. The activation of microorganisms such as Acinetobacter is noted. In addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. Anaerobic infection is seedy in 30.4% of patients' blood samples. Great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. The authors' investigations have shown that highly adhesive Staphylococci are common in severe pathological processes. The adhesiveness of the bacteria has been shown to depend on environmental conditions and the patient's status. Among the nonsporulating anaerobes there are bacteroids which are most highly adhesive.

Bacterial Adhesion↗

Bacterial arthritis in the adult.

A brief review of bacterial arthritis as seen at the Mayo Clinic in the years 1958 through 1973 has been presented with a selected literature review. Once considered a disease of children and adolescents, this disease now is seen more often in adults, especially in those who are elderly, are chronically ill, or are receiving medications that lower the host resistance in invading bacteria. The diagnosis must be made early, with rapid isolation and identification of the organism. Proper antibacterial drugs should be selected on the basis of in vitro susceptibility tests. The joint should be either aspirated or decompressed by closed suction-irrigation. In instances in which diagnosis is late and joint destruction occurs, joint débridement will be necessary.

Adult↗

Specificity of bacterial ribosomes and messenger ribonucleic acids in protein synthesis reactions in vitro.

Ribosomes from two Gram-negative bacteria translated f2 RNA, T4 early mRNA, mRNA from three Gran-negative bacteria, and mRNA from six Gram-positive bacteria; ribosomes from three Gram-positive bacteria translated mRNA from the Gram-positive strains, but did not translate the other mRNAs. Ribosomes from the Gram-negative bacterium Escherichia coli translated synthetic poly(U,G) but ribosomes from the Gram-positive bacterium Clostridium pasteurianum translated poly(U,G) very poorly, mRNA from Gram-negative bacteria was translated only in the presence of a high salt ribosomal wash containing initiation factors. mRNA from Gram-positive bacteria and synthetic poly(U,G) were translated much more efficiently when wash components were present, but were also translated to a small, but significant, extent in the absence of wash components. The translation specificity of each type of ribosome was independent of the source of ribosomal wash components. When the radioactively labeled products of in vitro protein synthesis were analyzed by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and autoradiography, it was found that each different bacterial and phage RNA preparation directed the synthesis of a unique set of polypeptide products of discrete sizes. Three different types of ribosomes were used to translate each of several Gram-positive bacterial messenger preparations; the overall patterns of products obtained with a given mRNA are similar, but some differences in the products formed or the relative amounts of the various products synthesized can be detected.

Azotobacter↗