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Determining optimal two-beam axial orientations for heart sparing in left-sided breast cancer patients.

BACKGROUND AND PURPOSE: The optimal intensity fluence profile of a beam depends on the profiles of other beams but most optimizations assume fixed beam orientations, a priori. Breast cancer radiotherapy attempts to cover the target and to spare critical structures such as the heart and lungs. The study aims are (1) to determine and document the optimal two-beam orientation that best spares the heart for left-sided breast cancer patients and (2) to investigate the influence of the treatment technique (i.e., conformal versus intensity modulation) on the optimal objective cost function. MATERIAL AND METHODS: Ten left-sided breast cancer patients were planned using a conformal (3DCRT) and a simplified intensity modulated (sIMRT) technique using predefined segments and different two-beam orientations. Optimal segment weights were determined exhaustively for all axial two-beam combinations, in 5 degree increments, by minimizing a quadratic objective cost function. The resulting objective cost function was analyzed with respect to target geometry and treatment technique. RESULTS: The sIMRT plans are generally less sensitive to beam orientation compared to 3DCRT plans. Optimal two-beam orientations for 3DCRT and sIMRT plans exist and they correspond to a hinge angle of approximately 188 degrees and 160 degrees or 210 degrees (the latter is bimodal), respectively. CONCLUSIONS: The optimization software is a useful tool that can test many different beam combinations and estimate their associated objective cost values. Afterwards, the most promising beam orientations could be re-optimized under the TPS to fine-tune and verify the dose distributions. Optimal uniform two-beam orientations for the breast consist of opposing tangential medial and lateral beams. Optimal nonuniform two-beam orientations for left-sided breast cancers are bimodal, containing hinge angles around 160 degrees and 210 degrees. Nonuniform beam techniques are less sensitive to beam orientation compared to uniform beam techniques and result in significantly improved heart sparing but at a cost of slightly compromised planning target volume coverage.

Breast↗

Classifying illusory contours by means of orientation discrimination.

1. Thresholds for discrimination of orientation were measured for solid and interrupted lines, and for illusory borders defined by offset abutting lines of a variety of configurations. 2. Illusory contours defined by orthogonal lines with a clear gap or overlap manifest excellent orientation discrimination even for very short exposure durations, and are robust to pattern motion and masking. The performance is almost as good as that for real lines. 3. When the contour is made up of abutting lines without gap or overlap, orientation discrimination is not nearly as good, and deteriorates further with short exposure duration, with target motion, and with masking. 4. The introduction of disparity in such illusory borders does not improve the discrimination of its orientation, so that concepts such as surface elaborations do not yet have to be invoked. 5. The results suggest that there is one class of illusory contour whose orientations can be discriminated by virtue of its delivering adequate stimuli to orientation-selective units in primary visual cortex. For contours with a uniform space-averaged luminance, the orientation discrimination is helped by the orthogonality of the generating lines, and this gives support to the concept that orthogonally oriented end-stopped neurons may participate in the orientation discrimination.

Discrimination, Psychological↗

Sexual orientation in a U.S. national sample of twin and nontwin sibling pairs.

OBJECTIVE: Although previous studies have suggested that sexual orientation is influenced by familial factors, which may be partly genetic, these studies have relied on unrepresentative and potentially biased samples. The authors attempted to estimate the role of genetic and environmental factors in the determination of sexual orientation in a more representative sample. METHOD: Sexual orientation was assessed by a single item on a self-report questionnaire in a U.S. national sample of twin and nontwin sibling pairs. Sexual orientation was classified as heterosexual or nonheterosexual (bisexual or homosexual). The authors compared the similarity of sexual orientation in the monozygotic twins to the similarity in the same-sex dizygotic twins, all dizygotic twins, the same-sex dizygotic twins and sibling pairs, and all dizygotic twins and sibling pairs. Biometrical twin analyses were performed. RESULTS: All analyses demonstrated familial resemblance for sexual orientation. Resemblance was greater in the monozygotic twins than in the dizygotic twins or in the dizygotic twins plus nontwin siblings. Biometrical twin modeling suggested that sexual orientation was substantially influenced by genetic factors, but family environment may also play a role. No evidence was found for a violation of the equal-environment assumption regarding monozygotic and dizygotic twin pairs. CONCLUSIONS: Familial factors, which are at least partly genetic, influence sexual orientation. The results of these analyses should be interpreted in the context of low statistical power and the use of a single item to assess the complex phenotype of sexual orientation.

Adult↗

Primary ciliary dyskinesia syndrome associated with abnormal ciliary orientation in infants.

Primary ciliary dyskinesia (PCD) syndrome associated with abnormal ciliary orientation but with normal ciliary ultrastructure has been described in adults, but there are no normal ranges for orientation in infants, despite the fact that half of all patients with PCD present in the new-born period. Nasal brush biopsies were obtained from eight infants (three males), mean age 13.1 months, range 7-23, in order to determine ciliary orientation. They had no upper or lower airway disease and normal organ arrangement and were undergoing general anaesthesia for other reasons. Two infants with typical PCD syndrome but normal ultrastructure of individual cilia also had orientation studies. In the eight normal subjects, a mean of 254 central pairs was examined, range 82-453. The mean ciliary orientation was 14.9 degrees, range 12.9-17.5. The two infants with PCD syndrome but normal ultrastructure of individual cilia had ciliary orientation of (Case 1) 44.5 degrees (range 10.6-64.5) in 218 central pairs; and on a second occasion, 28.9 degrees, (range 9.0-47.5) in 259 central pairs; for Case 2, 24.4 degrees, (range 13.1-38.4) in 196 central pairs. The normal range for ciliary orientation is similar in infants to that described in other work in adults. The two cases of phenotypic primary ciliary dyskinesia in the presence of normal ciliary ultrastructure but abnormal ciliary orientation in infants supports the contention that measurement of ciliary orientation should be part of the assessment of ciliary structure and function in cases of possible primary ciliary dyskinesia, in particular when the ultrastructure of individual cilia appear to be normal.

Cilia↗

Orientation determination by wavelets matching for 3D reconstruction of very noisy electron microscopic virus images.

BACKGROUND: In order to perform a 3D reconstruction of electron microscopic images of viruses, it is necessary to determine the orientation (Euler angels) of the 2D projections of the virus. The projections containing high resolution information are usually very noisy. This paper proposes a new method, based on weighted-projection matching in wavelet space for virus orientation determination. In order to speed the retrieval of the best match between projections from a model and real virus particle, a hierarchical correlation matching method is also proposed. RESULTS: A data set of 600 HSV-1 capsid particle images in different orientations was used to test the proposed method. An initial model of about 40 A resolutions was used to generate projections of an HSV-1 capsid. Results show that a significant improvement, in terms of accuracy and speed, is obtained for the initial orientation estimates of noisy herpes virus images. For the bacteriophage (P22), the proposed method gave the correct reconstruction compared to the model, while the classical method failed to resolve the correct orientations of the smooth spherical P22 viruses. CONCLUSION: This paper introduces a new method for orientation determination of low contrast images and highly noisy virus particles. This method is based on weighted projection matching in wavelet space, which increases the accuracy of the orientations. A hierarchical implementation of this method increases the speed of orientation determination. The estimated number of particles needed for a higher resolution reconstruction increased exponentially. For a 6 A resolution reconstruction of the HSV virus, 50,000 particles are necessary. The results show that the proposed method reduces the amount of data needed in a reconstruction by at least 50 %. This may result in savings 2 to 3 man-years invested in acquiring images from the microscope and data processing. Furthermore, the proposed method is able to determine orientations for some difficult particles like P22 with accuracy and consistency. Recently a low PH sindbis capsid was determined with the proposed method, where other methods based on the common line fail.

Algorithms↗

Disclosure of sexual orientation and behavior in the Nurses' Health Study II: results from a pilot study.

OBJECTIVE: To examine disclosure of sexual orientation and response rates in a pilot study of questions on sexual orientation and gender of sexual partners in the Nurses' Health Study II. METHODS: A pilot questionnaire was mailed to random samples of 350 women from each of three marital status strata: never married, previously married, and currently married. We estimated prevalence of each category of sexual orientation in the pilot study. Response rates to the sexual orientation question for the pilot questionnaire and the 1995 cohort questionnaire were compared. RESULTS: The overall response rate to the pilot study was 78%. In the pilot study, 98% of women reported a sexual orientation of heterosexual, 0.1% bisexual, and 0.9% lesbian, with 0.7% either declining to answer, leaving the question blank, or categorizing themselves as none of the above, weighted for stratified sampling by marital status. The distribution of sexual orientation in the cohort study (N = 91,654) was similar. Asking women to participate in the pilot study of sexual orientation questions did not appear to cause participants to drop out of the cohort. Concordance between reported sexual orientation on the pilot study and the cohort study was high. CONCLUSIONS: Based on our experience, researchers may be encouraged to add questions on sexual orientation to large studies of women.

Bisexuality↗

Relationship among achievement goal orientations and multidimensional situational motivation in physical education.

BACKGROUND: Contemporary research suggests that task and ego achievement goal orientations affect students' intrinsic motivation in physical education. This research has assessed intrinsic motivation as a unidimensional contruct, however, which is inconsistent with the more contemporary postulates of self-determination theory (Deci & Ryan, 1985, 1991) which states that intrinsic motivation is only one type of motivation. To date, research has not addressed whether different types of motivation at the situational level are influenced by the proneness to adopt task or ego involvement. AIMS: To examine the relationship between achievement goal orientations and multidimensional situational motivation in PE. SAMPLE: Middle school children (182 male, 136 female; M age = 13.2 years). METHOD: Responded to questionnaires assessing their dispositional goal orientation (POSQ; Roberts, Treasure, & Balague, 1998) and situational motivation (SIMS; Guay, Vallerand, & Blanchard, 2000) in PE. RESULTS: Task orientation was found to be positively associated with more self-determined types of situational motivation. Ego orientation was weakly related to less self-determined motivation. An extreme group split was conducted to create four goal groups and goal profile analyses conducted. A significant MANOVA was followed by univariate analyses, post hoc comparisons, and calculated effect sizes, which revealed that groups high in task orientation reported more motivationally adaptive responses than groups low in task orientation. CONCLUSIONS: The results suggest that a high level of task orientation singularly or in combination with ego orientation fosters self-determined situational motivation in the context of PE.

Achievement↗

Determination of molecular orientation of uniaxially stretched polyamide fibers by polarized infrared spectroscopy: comparison of X-ray diffraction and birefringence methods.

Polarized infrared (IR) spectroscopy has been used to determine the crystalline and amorphous orientation of polyamide fibers. The transition moment angle of the band at 936 cm-1 of PA66 was determined to be 48 degrees using IR spectroscopy and birefringence measurement. The crystalline orientation of PA66 fibers was estimated from the band at 936 cm-1 while the amorphous orientation of PA66 fibers was obtained by an indirect method. The alpha crystalline orientation of PA6 has been obtained using the band at 930 cm-1 and the amorphous orientation of PA6 has been determined using the band at 1124 cm-1. Crystalline orientation increased rapidly at low draw ratios (DR<3) and increased slowly at higher draw ratios (DR>3) for both PA66 and PA6 fibers, while the amorphous orientation increased slowly throughout the whole extension range for PA66 fibers. A good correlation was found between the crystalline orientation values obtained by infrared spectroscopy and other methods such as X-ray diffraction for PA66 and PA6 fibers. On the basis of this observation, it has been concluded that polarized infrared spectroscopy can be used reliably to measure the orientation of polyamide fibers without combining with other techniques.

Birefringence↗

Orientation-insensitive spectra for Raman microspectroscopy.

Separating effects due to molecular conformation from those due to orientation in the spectra of oriented samples obtained by Raman microspectroscopy is a complex issue. To solve this problem, we propose a procedure to calculate an orientation-insensitive spectrum (so-called isotropic spectrum) from polarized spectra obtained by Raman microspectroscopy that is valid for systems that exhibit a uniaxial symmetry. The method has first been tested on highly oriented samples of high-density polyethylene (HDPE). Polarized and isotropic spectra of a highly oriented HDPE cylindrical rod and an isotropic HDPE sample have been compared. The differences in the relative intensities, which occur in the polarized spectra and are due to orientation of the polyethylene chains, are nearly cancelled in the isotropic spectra, showing that the orientation-insensitive spectrum adequately represents the molecular conformation without contributions of orientation. Second, spectra of silk fibroins have been compared in the amide I region for Bombyx mori cocoon silk fibers and methanol-treated regenerated fibroin films. The similarity of the shape of the amide I band of the isotropic spectra indicates that the secondary structure of the fibroins is very close in both samples. These experimental results support the conclusion that the molecular conformation can be efficiently characterized from the intensity and the shape of Raman bands in the orientation-insensitive spectrum.

Animals↗

Application of the critical pathway and integrated case teaching method to nursing orientation.

BACKGROUND: Nursing staff development programs must be responsive to current changes in healthcare. New nursing staff must be prepared to manage continuous change and to function competently in clinical practice. METHOD: The orientation pathway, based on a case management model, is used as a structure for the orientation phase of staff development. The integrated case is incorporated as a teaching strategy in orientation. The integrated case method is based on discussion and analysis of patient situations with emphasis on role modeling and integration of theory and skill. RESULTS: The orientation pathway and integrated case teaching method provide a useful framework for orientation of new staff. Educators, preceptors and orientees find the structure provided by the orientation pathway very useful. CONCLUSION: Orientation that is developed, implemented and evaluated based on a case management model with the use of an orientation pathway and incorporation of an integrated case teaching method provides a standardized structure for orientation of new staff. This approach is designed for the adult learner, promotes conceptual reasoning, and encourages the social and contextual basis for continued learning.

Adult↗

Relationship of anxiety level and recall of information to the interval between orientation and first patient care day.

Sophomore students' responses to two different intervals between orientation and first clinical day in medical/surgical nursing were examined. Variables studied were anxiety state and ability to recall information which had been presented. Prior to orientation all students were given the Spielberger STAI-S and STAI-T anxiety scales. At the beginning of the first laboratory session the students completed the STAI-S scale and a teacher-made quiz on information presented in orientation. Students in Group I (n = 51) had their first laboratory one week after orientation. Group II (n = 39) had their first laboratory the day following orientation. There was no significant difference between the two groups on cumulative grade point average, beginning STAI-T or STAI-S. Likewise, there was no significant difference between the scores made on the quiz. (The validity and reliability of the quiz were not determined.) There was a significant difference between the two groups on changes in state anxiety from orientation to the first laboratory. Group I (laboratory one week after orientation) was more anxious than Group II (laboratory one day after orientation) (p = 0.024). Both groups were significantly more anxious in the Spring than Fall quarter (p = 0.034). It was concluded that anxiety related to a new clinical experience might be lessened if the first clinical day closely followed orientation. It was also noted that students may be more anxious in the Spring than in the Fall of the year.

Anxiety↗

[Development of a computerized method for identifying view position and orientation for chest radiographs by using a template matching technique].

For implementation of computer-aided diagnostic systems for chest radiographs in the clinical environment, it is necessary to correctly identify two view positions [posteroanterior (PA) and lateral views] and four orientations (upward, downward, leftward, and rightward) for chest radiographs. In picture archiving and communication system (PACS), the information on the view position and orientation for chest radiographs is often not recorded or is recorded incorrectly. The purpose of this study was to develop a computerized method for correctly identifying view position and orientation for chest radiographs by using a template matching technique. Our basic approach is to find the most similar template to an unknown chest image by examining the similarity of the unknown chest image with a number of templates for PA and lateral views at the four image orientations, and to determine simultaneously the view position and orientation for the unknown chest image. All upward templates were produced from the PA or lateral images for upward orientation for various patient sizes. By rotating the upward templates, we produced the templates for downward, leftward, and rightward orientations. To evaluate the similarity of a test image with all templates, the cross-correlation values of the test image with all templates were obtained. The view position and image orientation for the unknown image was considered to be identical to those for the template with which the largest cross-correlation value was obtained. Our results indicated that all cases of 200 PA and 200 lateral views were correctly identified in terms of the view position and image orientation.

Diagnosis, Computer-Assisted↗

Right from the start: the family practice orientation study.

BACKGROUND AND OBJECTIVES: Orientation to a residency program introduces residents to the essential functions of their duties, clarifies expectations, and acclimates them to a new social group. This study identified the orientation goals of family practice residency directors and compared those goals to the perceived needs of first-year family practice residents. METHODS: We surveyed program directors and PGY-1 residents at 100 accredited family practice residency programs. Questionnaires obtained descriptive information about orientation programs and resident demographics and assessed directors' goals and residents' needs from orientations. RESULTS: The orientation goal ranked most highly by directors was development of group cohesion. The need ranked most highly by residents was introduction to call duties and inpatient problems. Ranked responses of each group were not well associated. CONCLUSIONS: The orientation goals of program directors and the reported orientation needs of residents were poorly associated. Most directors chose nonclinical areas as their most important goals for orientation. In contrast, most residents valued clinically related information during orientation to residency.

Family Practice↗

[Use of cellulose acetate millipore filters for the correct orientation of endoscopic biopsies in digestive diseases].

INTRODUCTION: Properly oriented endoscopic biopsies allow an effective assessment of some diagnostic features in non neoplastic gastrointestinal diseases. MATERIALS AND METHODS: We used cellulose acetate millipore filters (Endofilter, Bio-Optica, Milan, Italy) in order to improve the orientation of biopsies during processing. Forty biopsies were placed on filter after endoscopic sampling whereas no orientation attempt was done for other 40 filter-free biopsies (20 from esophagus and 20 from gastric antrum in each group). Both groups were compared in terms of orientation and assessability of the following morphological features: thickness of basal layer in the esophageal squamous epithelium, length of esophageal papillae, interstitial space dilatations in the esophageal squamous epithelium and gland atrophy in the gastric antrum. Both orientation and assessability of individual morphological features were graded with a score ranging from 1 (good) to 3 (poor). The impact of this procedure on costs was analysed, both in terms of material and technical workload. RESULTS: All 20 esophageal and 20 antral biopsies on filter showed acceptable (score 1 or 2) orientation. In contrast, 14/20 filter-free esophageal and 13/20 antral biopsies showed poor (score 3) orientation (p = 0.0001 for both groups). Basal layer thickness was assessable (score 1 or 2) in 20/20 esophageal biopsies on filter vs 14/20 filter-free ones (p = 0.0001) and length of papillae in 15/20 biopsies on filter vs 4/20 filter-free ones (p = 0.0002). Interstitial space dilatation assessability was not affected by orientation procedures. Gland atrophy in the antrum was assessable (score 1 or 2) in 20/20 gastric biopsies on filter vs 8/20 filter-free ones (p = 0.0001). The use of endofilters permitted the process of numerous (up to 8 for each block) samples from different biopsy sites together and produced a significant reduction in costs (18.35 in the case of 8 biopsies from 4 different biopsy sites). CONCLUSIONS: The use of millipore filters allows orientation of biopsy samples, improves the assessment of several diagnostic features in esophageal and gastric pathology and yields a significant reduction in costs when biopsies from different sites are processed together.

Atrophy↗

[A geometrical model for the perceived line of orientation based on subjective evaluations and human VEP data].

A geometric model in proposed based on subjective dissimilarity estimates among differently oriented line segments in the frontal plane and on visual evoked potentials (VEP) recorded in respond to abrupt changes in the orientation of such a line segment. The orientations are represented by points constituting a clised planar curve, with interpoint distances corresponding to interstimulus dissimilarities. The angle of the radius-vertor connecting the center of the configuration with its circumference encodes the orientation of the line stimuli in the visual field, and two Cartesian axes drawn through the center are interpreted as two orientation-opponent channels in the neuronal network processing line orientation. The first channel (the ordinate axis) gives maximal positive and maximal negative responses to, respectively, vertical and horizontal orientations, whereas the second channel (the abscissa axis) gives maximal positive and maximal negative responses to, respectively, 45 and 135 degrees orientations. The VEP analysis shows that the activity of the first channel affects the interpeak amplitudes of both the P1-N2 (P130-N180) and N2-P2 (N180-P230) components, whereas the second channel affects only the late component N180-P230. We propose the hypothesis according to which outputs of the two channels are connected linearly (which is reflected in the city-block metric obtained when distances are computed directly from neuronal activity), but the growth rate of the overall output is inhibited nonlinearly when it reaches large absolute values. This inhibition effectively transforms the "true" city-block metric of the line orientation space into the Euclidean metric one obtains when distances are computed from subjective dissimilarity estimates. VEP amplitudes as intermediate characteristics of visual processing between neuronal activity and subjective estimates represent intermediate metric (between city-block and Euclidean).

Adult↗

Orientation response of arterial smooth muscle cells to mechanical stimulation.

Arterial smooth muscle cells from rabbit aortic media in primary culture and subculture were grown on hydrophilized and collagen-coated silicone membranes which were then subjected to cyclic and directional stretches and relaxations at a frequency of 60 times/min. The membranes were stretched with various amplitudes ranging from 2% to 20%. Smooth muscle cells on unstretched membranes in the same incubation chamber served as controls. In long-term experiments the stretching and relaxing of the membranes was continued for several days. While the smooth muscle cells grown on unstretched membranes remained in random orientation in all experiments, the cells which underwent mechanical stimulation showed a high degree of orientation. The angle of cell orientation varied in direct relation to the stretching amplitude and became steeper in correlation to the intensity of the mechanical stimulus. The angle of cell orientation was reversible, as preoriented cells changed their orientation when another stretching amplitude was applied. To study the role of cytoskeleton in the process of cell orientation, we examined the behaviour of the intracellular actin filament system. In short-term experiments the smooth muscle cells were exposed for 3 to 12 h to cyclic and directional stretches and relaxations with an amplitude of 10%. We observed a rearrangement of the intracellular actin filament system prior to the orientation of the whole cell bodies. The present study provides evidence that stretching the artery wall by blood pulsation may result in an orientation response of the intracellular actin cytoskeleton and in the orientation of the smooth muscle cells within the media of artery walls.

Actins↗

Mechanism of the anisotropic dimensional change of the wax pattern prepared by the softened wax technique. (1) Relationship between recovery and crystal orientation.

In order to obtain information on the behavior of wax pattern distortion and to reveal its mechanism, the following experiments have been conducted: measurements of fundamental properties including distribution of molecular weight, crystal structure, phase transition, and viscosity, and investigation of the crystal orientation and recovery phenomena. If the wax is deformed plastically at higher temperatures, transformation appears to be a smooth transition from an isotropic distribution of the crystal orientation to an arranged structure. In the case of uniaxial compression, the c-axes are rearranged parallel to the compressed direction and the b-axes and a-axes are randomly oriented in the plane normal to the c-axes. This preferred orientation is due to the crystal rotation. The degree of preferred orientation increases with the increase in compression ratio over all temperatures between 35 degrees C and 55 degrees C. Above 45 degrees C, the degree of orientation decreases with an increase in temperature despite the equal compression ratio. From this fact, the degree of orientation is associated with the crystallinity at deformation temperatures as well as the degree of deformation. The preferentially oriented specimen expands greatly in the direction parallel to the molecular chain axis and shrinks to a great extent in the direction perpendicular to the chain axis. This anisotropy is caused mainly by the recovery. Moreover, the extent of recovery is associated closely with the degree of the preferred orientation. The dimensional change due to the recovery is extraordinarily large (30 to 40%) in comparison with the normal thermal expansion from room temperature to 45 degrees C (approximately 1.2%). This large extent of recovery is explained by the rubber-like deformation in the amorphous region.

Crystallization↗

A multivariate analysis of kinanthropometric profiles of elite female orienteers.

The purpose of this study was to examine the relevance of various kinanthropometric features of elite female orienteers in terms of success at the Student World Orienteering Championships (SWOC)(1992). Participants at this competition were divided into successful (final placing in top 35; n = 12) and less successful (final placing below 35; n = 11) competitors. A non-orienteering reference group was used to enable the identification of any sport specific factors. Measurements of the orienteering group were taken during the week of the SWOC and included 5 skinfold thicknesses, 2 limb girths, 2 bone breadths, 3 proportional lengths and 3 physical tests (grip strength, leg strength, flexibility). Using the results of these, percent adiposity, somatotypes and various anthropometric indices were also obtained. Age and adiposity variables were found to correlate significantly with competitive performance (p < 0.01). Discriminant function analysis successfully distinguished between the successful and less successful orienteers (Wilks' Lambda = 0.603; p < 0.01). There was no difference (p > 0.05) between the orienteering group and the reference group for the majority of the proportionality measures and similarily in the results of the physical tests. Adiposity values were higher in the non-orienteers. In conclusion, body composition measures discriminated between successful and less successful orienteers at a major event at an elite level. The primary difference between the orienteers and the reference group was in body composition rather than linear or proportional measures.

Adipose Tissue↗