Public health, public policy and "neon" issues in ethics.
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Blood obtained from 33 healthy donors was irradiated with He-Ne laser. During this process the count of segmented neutrophils decreased, while the content of radioimmunologically determined leukotriene B4 and thromboxane B2 increased in the other neutrophils. Application of mannose-specific lectins (Con A, PSL) as inductors caused the increase, while application of other carbohydrate-specific lectins caused the decrease of luminol-dependent chemiluminescence in neutrophilic granulocyte suspension after the irradiation. As a result of direct laser effect on the blood cells some portion of the less resistant cells were eliminated from the blood flow, i.e. "rejuvenation" of the neutrophilic granulocyte population in the cells takes place.
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The muscle regeneration was studied in across-cut gastrocnemius muscle of adult guinea pigs. Both hind guinea pig legs were exposed to laser therapy (3-9 procedures for 5 min each) after operation. It was shown that laser therapy accelerated fibrin resorption and wound healing, decreased the muscle fiber degeneration, increased DNA and RNA synthesis in regenerating tissues, stimulated regenerative muscle fiber capacity. However, the regenerating muscle tissue did not connect both muscle stumps, and the narrow connective tissue scar was formed. Before it was shown that the same laser therapy conditions were more effective for rats. Probably, the stimulating effect of laser rays on regenerative tissue ability depends on species peculiarities of animals.
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The psychological structure was studied in 120 patients with paroxysmal disorders of the cardiac rhythm. Anxiety, obsessive-phobic, neurasthenic and ergopathic syndromes were singled out. It was established that psychological aspects of personality of the patients depended on the course of paroxysmal disorders of the rhythm and influence the number of arrhythmia triggering that often reduced the grade of medicosocial adaptation. Psychological preparation of these patients to laser treatment may be achieved by rational psychotherapy. Details of such psychotherapy are discussed.
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Red laser light (lambda = 633 nm) in a dose of 9.5 mJ/cm2 defends quail's embryo from X-ray irradiation (8.5 Gr). It is expressed in the 1.9 times decrease of embryo mortality on early term of incubation and 1.6 times increase of hatch in a group of embryos, which were affected by X-rays and laser radiation compared with an embryo, irradiated only by X-rays. Repeated laser irradiation of adult quails (dose 21 J) after CCl4-intoxication led to normalization of hydroxylation and antioxidant systems functions.
The effectiveness of laser therapy in trophic skin disorders (ulcer, diabetic bulla, hematoma, skin chap) in patients with diabetes mellitus was studied. The foci of trophic disorders and biologically active points were irradiated by means of heliumneon laser installations. The technique was employed in a hospital and in out-patient treatment in the complex with medicamentous therapy and without that in patients resistant to medicamentous therapy. A high effectiveness of laser therapy has been established.
Low-power He-Ne laser irradiation causes a well-defined and energy dependent cell destruction of in vitro cultured cell lines sensitized by hematoporphyrin derivative (HPD). The mechanism of this photosensitization was studied by measuring with polarization microscopic, scanning electron microscopic, and electron-spin-resonance (ESR) spectroscopic parameters. The cell damage caused by photosensitization and laser irradiation seems to be a complex process, however the biological membranes seem to be one of the primary targets. The energy of laser light causes rotation and resonance changes of macromolecules and the water molecules, resulting in an increased structural order of the submembraneous components in the living cells, detectable microscopically. During the photosensitization process, the red (630 nm) He-Ne laser light, during a one-photon energy activation, causes excitation of hematoporphyrin molecules to their triplet state. The excitation of HPD molecules results in a multi-step, free-radical generating effect, measured by ESR spectroscopy and studied by the ultrastructural changes of membrane organization and cell shape. Similar effects could be observed on in vitro lipid-water liposome membranes.
The authors analyze the condition of free-radical oxidation and activity of antioxidant system, clinical effectiveness of He-Ne laser therapy of patients with chronic pyelonephritis. It is shown that clinical manifestation of the disease is accompanied with activation of free radical oxidation and hypoactivity of antioxidant system. Endovascular laser hemotherapy stimulates antioxidant system, activity of superoxide dismutase, in particular. A good response and immunomodulation are achieved.
Humoral autoimmunity of patients with malignant gastrointestinal tumors was studied by different variants of enzyme immunoassay. In the preoperative period the frequency of detecting autoantibodies binding to DNA and components of external and internal membranes of cells of different tissues was not higher in patients (n-55) than in donors (n-45). Beginning from the 7th postoperative day all patients were found to have a significantly increased level of autoantibodies to DNA (p less than 0.01), fibroblasts (p less than 0.01), hepatocytes (p less than 0.01), islet cells (p less than 0.01), hepatic or pancreatic microsomes (p less than 0.05). There was a tendency towards normalization of these level by the 21st day in all cases. In patients (n-10) treated by blood intravascular laser irradiation after the operation the level of IgG (but not IgM) autoantibodies to DNA by the 14th postoperative day was significantly (p less than 0.01) higher than that in patients whose blood was not exposed to radiation. It is suggested that the discovered humoral immune reaction is a necessary component of wound immunity and develops in response to hetero-organic autoantigens which are unmasked in injury of the tissues during the operation. Laser therapy ensures a more intensive development of this reaction without altering its direction.
He-Ne laser stimulation was used in multiple-modality treatment of 512 patients with various corneal conditions: 217 with perforating wounds, 75 with chemical and thermal burns, 48 with ulcers of various origins, and 162 with endothelial-epithelial dystrophy resulting from cataract extraction with implantation of the intraocular lens. A course of treatment consisted of 6 to 10 daily 3 min exposures at radiation power density of 50-100 micro W/cm2 on the cornea. The effect achieved by laser stimulation was significantly superior to that of routine methods of treatment used in the reference groups. He-Ne laser stimulation was conducive to a sooner reduction of the inflammatory processes, to recovery of the corneal sensitivity and epithelialization, and to shortening hospital treatment.
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