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Biochemical characterization of lysozymes present in egg white of selected species of anatid birds.

The isolation of lysozyme from the egg white of several representative species of waterfowl is described. The purified lysozymes were analyzed in order to determine the type and molecular weight of each enzyme. All enzymes found in duck egg whites were found to be of the c-type. In contrast all true geese, the Mute Swan as well as the Northern Blackneck Screamer contain lysozyme g in their egg white.

Animals↗

Biochemical characterization of lysozymes present in egg white of selected species of anatid birds.

The isolation of lysozyme from the egg white of several representative species of waterfowl is described. The purified lysozymes were analyzed to determine the type and molecular weight of each enzyme. All enzymes found in duck egg whites were found to be of the c-type. In contrast all true geese, and the mute swan species as well as the northern blackneck screamer contain lysozyme g in their egg white.

Animals↗

Molecular epidemiological analysis of Newcastle disease virus isolated in China in 2005.

Eighty-three strains of Newcastle disease virus (NDV) were obtained from outbreaks in chickens, pigeons, geese, and ducks in China in 2005 and characterized genotypically. The main functional region of the F gene (535 nucleotides) was amplified and sequenced. A phylogenetic tree based on nucleotides 47-435 of the F gene was created using sequences from 83 isolates and representative NDV sequences obtained from GenBank. Phylogenetic analysis showed that all newly characterized strains belonged to six genetic groups: I, II, III, VIb, VIIc, and VIId. All the isolates belonging to groups I and II (14 total) were lentogenic according to the amino acid sequences of the fusion protein cleavage site, and either V4 or LaSota-type, depending on the vaccines that were used. Most isolates (64 total) were classified in group VIId, a predominant genotype responsible for most Newcastle disease outbreaks since the end of the last century. One strain, NDV05-055, was in group VIIc, three pigeon strains were in group VIb, and one isolate, NDV05-041, was in group III, and characterized as a velogenic strain. This study revealed that genotype VIId was the major NDV strain responsible for the 2005 ND epizoonosis that occurred in China.

Animals↗

Daily variation in the concentration of melatonin and 5-methoxytryptophol in the goose pineal gland, retina, and plasma.

The goose pineal gland rhythmically produces two 5-methoxyindole compounds, namely melatonin and 5-methoxytryptophol. Melatonin concentrations were high at night and low during the day, while in contrast 5-methoxytryptophol levels were markedly higher during the day compared to the night-time values. Rhythmic oscillations in melatonin content, with high night-time values, have also been found in plasma and the retina of goose. The pineal and retinal melatonin rhythm mirrored oscillations in the activity of serotonin N-acetyltransferase (AA-NAT; the penultimate and key regulatory enzyme in the melatonin biosynthetic pathway). Acute exposure of geese to light at night markedly decreased melatonin levels in the pineal, plasma, and retina. In addition, this light exposure resulted in a significant increase in pineal 5-methoxytryptophol content. Our results demonstrate, for the first time, the ability of the goose pineal gland and retina to synthesise melatonin and 5-methoxytryptophol in a rhythmic manner.

Animals↗

The genome of goose hemorrhagic polyomavirus, a new member of the proposed subgenus Avipolyomavirus.

The full-length genome of goose hemorrhagic polyomavirus (GHPV), the ethiologic agent of hemorrhagic nephritis and enteritis of geese, was cloned and sequenced. Transfection of the circular ds DNA with a size of 5256 bp and an organisation typical of polyomaviruses produced viral progeny in cultured goose cells. According to the splicing sites determined by RT-PCR, five open reading frames (ORFs) were found to encode putative proteins with significant similarities to large T antigen and small t antigen as well as VP1, VP2, and VP3 of other polyomaviruses. An additional ORF located in the 5' region of late mRNA, with a coding capacity for 169 amino acids, shows a low degree of homology to VP4 of avian polyomavirus (APV). The alignment of nucleotide sequences and amino acid sequences revealed a relatively close relationship between GHPV and APV. Therefore, grouping of this new polyomavirus into the proposed subgenus Avipolyomavirus is suggested.

Amino Acid Sequence↗

An effective method for freezing White Italian gander semen.

Efficiency of freezing method, worked out for the White Italian gander semen was evaluated by comparing motility, morphology and fertilizing ability of spermatozoa in fresh and frozen-thawed semen. A part of pooled semen, collected from 25 White Italian ganders by dorso-abdominal massage was used immediately for artificial insemination of 10 geese (the control group) with a dose of 80 microl. This insemination was performed six times at weekly intervals. The remainder of the semen was diluted 1:0.5 (v/v) with EK diluent, equilibrated for 15 min at +4 degrees C, mixed with 6% (v/v) of dimethylformamide (DMF) and frozen to -140 degrees C at a rate of 60 degrees C/min. Frozen semen was thawed in a 60 degrees C water-bath and inseminated twice weekly in a dose of 100 microl (10 females of the experimental group, 12 inseminations were made). The freezing process affected spermatozoa motility and morphology, but had no effect on their fertilizing ability. Positive movement was observed in 50-60% of the spermatozoa in fresh semen and about 40% of the frozen-thawed cells. The average percentage of total live and live normal spermatozoa decreased due to freezing from 92.2 to 68.4% and from 34.7 to 14.1%, respectively. After the fresh semen insemination with average 12 million of the live normal spermatozoa per week average fertility was 88.24%; hatchability of set eggs was 80.88% and hatchability of fertile eggs was 91.67%. For frozen-thawed semen inseminated with average 9.5 million of the undamaged spermatozoa per week, the average fertility and hatchability rate was 83.78, 73.87, and 88.17%, respectively. Fecundity rates obtained after insemination with the frozen-thawed gander semen allow for the application of the freezing technique into breeding practice, in place of natural mating or to assist natural mating in periods of lowered fertility level.

Animals↗

Avian immune responses to Mycobacterium avium: the wildfowl example.

Immunological responses of wildfowl (Order Anseriformes: ducks, geese, swans and screamers) to mycobacteria have been investigated as part of studies to develop a vaccine and diagnostic assay for avian tuberculosis. 10(9) killed Mycobacterium vaccae protected the Cairinini (perching ducks) from avian tuberculosis (p<0.02) but did not achieve statistically significant protection in the other taxonomic tribes. The Cairinini includes the threatened, yet highly susceptible, white-winged duck (Cairina scutulata).Together, loss of cell-mediated responses to common mycobacterial antigens, increased responsiveness to the species specific antigens of M. avium, and increased antibody production are reminiscent of the T(H1) to T(H2) shift seen in mammalian mycobacterial infections. It is speculated that excessive exposure to environmental mycobacteria prior to vaccination is detrimental and common antigens play an important role in wildfowl immunity to mycobacteria. A new vaccination trial using killed M. vaccae is being undertaken. Antibody responses are a useful ante mortem diagnostic indicator in most taxonomic tribes with the exception of the primitive Dendrocygnini (whistling ducks).

Animals↗

Fluorescent and electron-microscopy immunoassays employing polyclonal and monoclonal antibodies for detection of goose parvovirus infection.

Polyclonal antibodies (PAbs) raised in geese and eight mice hybridomas secreting monoclonal antibodies (MAbs) against the goose parvovirus (GPV) were prepared. They were used for development of immunofluorescence (IF) and immunoelectron-microscopic (IEM) techniques to demonstrate the GPV infection in infected organs and biological fluids. The GPV antigens were established by immunofluorescence within the nuclei and the cytoplasm of many infected cells of the chorioallantoic membrane of goose and Peckin duck embryos, liver and heart of mortally diseased goslings. By means of IEM it was possible to detect the GPV in native organ homogenate supernatants and allantoic fluids. All techniques used in the study could be successfully applied for rapid diagnosis of the GPV infection. The test systems on the basis of MAbs should, however, be preferred. By means of immunoblotting (IB) using PAbs and MAbs four viral proteins (VP) with MW 88, 77, 65 and 60 kDa were demonstrated. Contrary to the others the VP with MW 65 kDa was the most antigenically reactive though invisible in the SDS-PAGE and Coomassie-blue dye-stained preparations.

Animals↗

Is osteonecrosis of the femoral head avascular? Bone blood flow measurements after long-term treatment with corticosteroids.

Since nontraumatic osteonecrosis of the femoral head is usually considered primarily as an ischemic disease, blood flow has been studied in femoral segments in two groups of geese, one control group and the other treated with a high dose of corticosteroids for 5 months. There was no histologic evidence of osteonecrosis in femoral heads, and mean bone blood flow in any femoral segments was not reduced in the treated group. These results suggest that osteonecrosis of the femoral head is not caused primarily by ischemia, even if ischemia may play a secondary role in the evolution of the disorder.

Animals↗

Avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses.

Mixed infections involving mycoplasmas, viruses and bacteria are well recognized in chickens. Synergism has been demonstrated between Mycoplasma gallisepticum and the viruses of Newcastle disease and infectious bronchitis and Escherichia coli, although the outcome of infection is influenced by many factors associated with the host and the organisms. Airsacculitis in broilers due to M. synoviae or M. gallinarum may be precipitated by concurrent respiratory virus infections including vaccine strains. Turkeys, geese and ducks have been less well studied, but similar interactions seem to occur. Such observations may give an indication as to the likely interactions between mycoplasmas, viruses and bacteria in other host species.

Air Sacs↗

Hypersensitivity pneumonitis resulting from community exposure to Canada goose droppings: when an external environmental antigen becomes an indoor environmental antigen.

BACKGROUND: In the past, hypersensitivity pneumonitis has been attributed to occupational, agricultural, or home environmental exposure. OBJECTIVE: This report describes the first case of hypersensitivity pneumonitis due to community exposure to droppings from Canada geese migrating through a suburban environment. METHOD: Clinical and serologic information was used in making the diagnosis of hypersensitivity pneumonitis. RESULTS: Serologic analysis demonstrated precipitating antibodies against goose droppings and against an extract made from washings from a filter taken from the patient's office. These studies also showed that the antigens in the office filter were goose dropping antigens. CONCLUSION: Hypersensitivity pneumonitis can result from exposure to goose dropping antigens in the community that enter buildings through ventilation systems. This represents a new form of an old disease.

Air Pollution, Indoor↗

Observations of basic diagnostic profile patterns seen in some common disorders of backyard poultry species.

Poultry species--chickens, ducks, geese--are becoming increasingly popular as pets. As such, requests for accurate diagnoses and treatment are being received by the veterinary community from the public. Unlike the food animal and production-oriented aspects of poultry medicine, success with these pet birds is contingent on preserving the human-pet bird bond, as defined by the individual client. This article presents some of this author's observations in diagnostic profiles on some selected disorders of backyard poultry.

Animals↗

Heart rate and rate of oxygen consumption during flight of the barnacle goose, Branta leucopsis.

We report the first measurements of heart rate (f(H)) and the rate of oxygen consumption (V(O(2))) during flights from a species of bird larger than 500 g. V(O(2))was obtained from nine forward flapping flights of 8.9 min mean duration at a mean speed of 13.2 m s(-1) performed by three barnacle geese of mean mass 1.68 kg. Mean V(O(2))was 332 ml min(-1)or 201 ml min(-1) kg(-1). Sixteen flights were obtained from two of these birds equipped with heart rate data loggers, both when they were wearing a V(O(2)) mask and when they were not. During flights with the mask (mean duration 7.4 min), mean f(H) was 472 beats per min and during flights without the mask (mean duration 8.0 min) it was 391 beats per min. Heart rate was also measured in another goose flying without a respiratory mask and mean f(H) for all the three birds (mean mass 1.7 kg) flying without a mask for an average of 7.9 min at 13 m s(-1) was 378 beats per min. Resting f(H) for these three birds was 79 beats per min. The values of f(H) during flight are greater than those obtained from the same species during their autumn migration from Spitsbergen to southern Scotland. The possible reasons for this are discussed.

Animals↗

Pancreatic hormones and plasma glucose: regulation mechanisms in the goose under physiological conditions. II. Glucose-glucagon and glucose-insulin feed-back mechanisms.

We have studied the pancreatic hormone-glucose feed-back mechanisms by infusing glucagon (G), insulin (I) and glucose into normal fasting geese. The controls received saline. Whilst a NaCl 9% infusion is devoid of effect, the pancreatic hormones, used at physiological doses, modify the plasma glucose level, glucagon being hyperglycaemic and insulin hypoglycaemic. In addition, a physiological increase in plasma glucose provokes a drop in plasma glucagon and a rise in plasma insulin, thus a marked decrease in the G/I ratio. The results show that the pancreatic hormone-glucose feed-back mechanisms are effective under physiological conditions.

Animals↗

Pancreatic hormones and plasma glucose: regulation mechanisms in the goose under physiological conditions. I. Pancreatectomy and replacement therapy.

Measurements of plasma GLI and IRI in normal fasting geese, before and during constant I.V. infusion of saline, gave GLI/I ratios of 1.32 +/- .07 and 1.34 +/- .03 (w/w). As total pancreatectomy markedly reduces the pancreatic hormone level, leading to a mortal hypoglycaemia, we attempted to maintain plasma glucose within the normal range by constant I.V. infusion of glucagon and insulin into operated animals. The results as follows: 1. Blood glucose levels can be maintained within the normal range during experiments lasting 6 or more hours with a constant G/I ratio. 2. The G/I ratio obtained in operated animals (.96 +/- .12) is near to, but significantly lower (p less than .005) than, the GLI/I ratio measured in normal animals. This difference may be explained by the presence of a small amount of circulating gut GLI in the 2nd group.

Animals↗

Glucose, insulin and glucagon in the diabetic goose.

The relationships between plasma glucose, insulin and glucagon were studied in geese made diabetic by subtotal pancreatectomy. As early as the first hours after the operation, the plasma glucose increases and a permanent diabetes develops. This diabetic state is characterized by two features: a very low plasma insulin level, which does not vary during the survival of the diabetic animals and a concentration of plasma glucagon (of pancreatic origin) which transiently diminishes then rises far above the normal level, and is correlated with the basal concentration of plasma glucose. The impaired glucose tolerance observed in diabetic animals is related to the suppression of the glucose-insulin and glucose-glucagon feedback mechanisms.

Animals↗

Effect of insulin on the response of gut glucagon-like immunoreactivity (GLI) to oral glucose and food ingestion in the goose.

Gut GLI levels were measured in the plasma of normal, totally and subtotally depancreatized geese, using an antiserum specific for avian pancreatic glucagon and another one which crossreacts with intestinal extracts. Gut GLI was determined by difference between "total" GLI and immunoreactive pancreatic glucagon (IRG). Glucose given orally or a meal rich in carbohydrates elicited an elevation in plasma gut GLI. The increment of gut GLI was greater when the pancreas was removed. The over-stimulation of gut GLI was corrected by the administration of insulin. This is the first study which reports a correlation between insulin and gut GLI secretion in birds.

Animals↗

Genetic diversity enhances the resistance of a seagrass ecosystem to disturbance.

Motivated by recent global reductions in biodiversity, empirical and theoretical research suggests that more species-rich systems exhibit enhanced productivity, nutrient cycling, or resistance to disturbance or invasion relative to systems with fewer species. In contrast, few data are available to assess the potential ecosystem-level importance of genetic diversity within species known to play a major functional role. Using a manipulative field experiment, we show that increasing genotypic diversity in a habitat-forming species (the seagrass Zostera marina) enhances community resistance to disturbance by grazing geese. The time required for recovery to near predisturbance densities also decreases with increasing eelgrass genotypic diversity. However, there is no effect of diversity on resilience, measured as the rate of shoot recovery after the disturbance, suggesting that more rapid recovery in diverse plots is due solely to differences in disturbance resistance. Genotypic diversity did not affect ecosystem processes in the absence of disturbance. Thus, our results suggest that genetic diversity, like species diversity, may be most important for enhancing the consistency and reliability of ecosystems by providing biological insurance against environmental change.

Animals↗