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Rebuilding of microvascularization following surgical gingival elimination by split flap. Study by perfusion and diaphanization.

1. Intra-arterial vital perfusion was used in dogs to study the rebuilding of the large amount of gingiva and its vascularization, which were removed surgically by split flaps. 2. Visualization of the reconstruction of the microvascularization could be observed through thick (400 mu) sections cleared in methyl salicylate. 3. The periosteal vessels and the vascular plexi of the dentogingival junction were the only vessels remaining after the surgery, and served as progenitors for the rebuilding of the vascularization of the new soft tissue complex. 4. After 48 hours the cut vessels were closed and new capillaries were beginning to form. An intense superficial vascularization was connected by multiple vessels to the periosteal vasculature (4 days), showing the largest number of superficial blood vessels after 7 days, when the area was primarily re-epithelialized.

Animals↗

The destructive potential of electrosurgery on the periodontium.

Three cases have been presented illustrating the complications which may arise from the use of electrosurgery. These cases provide clinical evidence of the potential for severe periodontal destruction, a potential that must be weighed carefully by any practitioner who uses an electrosurgical instrument. It is suggested that new scientific studies be initiated to establish basic principles that will eliminate the hazards that now exist in the routine use of electrosurgery.

Adult↗

Healing following simulated fiber retention procedures in rats.

Healing responses of a simulated fiber retention procedure in rats has been presented. In the area of attached, but severed supracrestal fibers, a loss of cells was observed in the initial stages of repair. Subsequently, connective tissue cells migrated into this area as did blood vessels. A very distinct interface was seen at about 1 week after surgery between the advancing soft tissues from the flap wound surface and the attached, but severed supracrestal fibers. By 4 weeks after surgery, such distinction was absent and supragingival fiber bundles appeared continuous from their cemental attachment over to the adjacent alveolar crest. However, no evidence of cementogenesis was seen at this site which suggests that these attached fibers were attached prior to surgery. The position of the new junctional epithelium appeared to be limited to the level of the retained supracrestal fiber fragments. However, in areas where cementum, and thereby attached fibers, had been removed during surgery, an altered epithelial adherence was usually seen. This altered adherence was in the form of a long junctional epithelium or a short junctional epithelium on top of parallel oriented collagen fibers. The latter also appeared to adhere to the tooth surfaces.

Animals↗

A comparison of a periodontal dressing and chlorhexidine gluconate mouthwash after the internal bevelled flap procedure.

A group of 15 patients requiring comparable bilateral internal bevelled flap procedures took part in a study to compare the clinical results achieved when a dressing or chlorhexidine mouthwash was used during the first postoperative week. Initial preoperative conditions were comparable. At the end of the first postoperative week, significantly more plaque accumulated and the sulcus bleeding index was significantly higher on the dressing treated side. At 1 month and 3 months postoperatively the sulcus bleeding indices were below preoperative levels for both treated sides with no significant differences between the sides. A significant and comparable reduction in pocket depths occurred following the two postoperative treatments. Subjectively recorded pain scores demonstrated that more pain was experienced on the dressing treated side, particularly during the first 4 postoperative days. More patients preferred the mouthwash as a postoperative treatment.

Adult↗

Longitudinal evaluation of the excisional new attachment procedure in humans.

Evaluation of the Excisional New Attachment Procedure (ENAP) was extended to 3 years postoperatively. Generally, there was an overall mean increase in sulcus depth of 0.5 mm; an overall mean increase in recession of 0.1 mm; and an overall mean decrease in the amount of previously gained new attachment of 0.6 mm between 1 year and 3 years after surgery. These values reflected a statistically significant, but clinically insignificant, difference in mean sulcus depth and mean attachment level between the two evaluation periods. Only one tooth site demonstrated clinical findings worse than those determined preoperatively. These results are similar to those of other longitudinal studies.

Epithelial Attachment↗

Techniques for the protection and coverage of the donor sites in free soft tissue grafts.

Palatal donor sites used for free soft tissue grafts are commonly left exposed during healing which occurs by secondary intention. This often results in postoperative discomfort and pain and in some cases apparent delayed healing of the donor sites. Various techniques for the protection and coverage of donor sites have been presented and the rationale, advantages and disadvantages of coverage of gingival wounds have been discussed.

Cyanoacrylates↗

A method for securing conservative block section in the mandibular anterior region.

A conservative method for removal of histologic block tissue sections has been presented. Its application permits the operator to conserve a portion of lingual alveolar bone in the mandibular anterior region to allow maintenance of a sufficient amount of ridge bone and tissue for construction of a functional and esthetic removable or fixed prosthetic appliance. This block section procedure may be modified to permit more conservation of human tissues in those studies involving human subjects.

Alveolectomy↗

The relationship between the mobility of human teeth and their supracrestal fiber support.

A method to study the relationship between the mobility of human teeth and their supracrestal fiber support was developed. The results of this study would indicate that: (1) The supracrestal fibers do not contribute significantly to the support of a normal premolar tooth. (2) As a human premolar tooth develops moderate to severe loss of osseous support, the relative contribution of the supracrestal fibers to the support of the tooth increases significantly.

Adolescent↗

The healing extraction site: a donor area for periodontal grafting material.

In cases of advanced periodontal disease, it is likely that vertical osseous defects will be present and that selected teeth will require extraction. The healing extraction site provides a source of vital osseous grafting material for the treatment of these infrabony defects. The preparation and grafting of the recipient site, as well as the management of the donor area, are described in detail. Three cases are presented which illustrate the efficacy of this type of therapy.

Alveolar Process↗

The healing of gingival wounds in miniature swine.

Standard wounds of simple incision and partial excision of the dentogingival junction were made in miniature swine. The healing from the 1st to the 14th day were described clinically and histologically, and the standardization of the wounds permitted quantitative and statistical analysis. In wounds that are closely adapted to the tooth, the epithelium of the new dentogingival junction contributes primarily to early wound strength recovery. This functional achievement probably would not occur with thick clots or hematomas, or with any displacement of the gingiva away from the tooth.

Animals↗

A longitudinal study of peridontal status comparing osseous recontouring with flap curettage. I. Results after 6 months.

The purpose this study was to test for short-term clinical differences in periodontal status after treatment with osseous recontouring and flap curettage in humans. Twelve systemically healthy patients with bilaterally similar marginal periodontal destruction received a standardized regime of presurgical therapy. The posterior segments of these patients were then treated with osseous recontouring and flap curettage. The investigators assigned the segments in one jaw at random to osseous recontouring and flap curettage, and then reversed the sides receiving surgical treatment in the opposing jaw. Postsurgical photographs and measurements for supragingival plaque, tooth mobility, gingival inflammation and periodontal attachment levels relative to the cemento-enamel junction were made for 6 months. Statistical analysis revealed that: (1) osseous recontouring and open flap curettage equally reduced plaque and gingival inflammation; (2) each surgical procedure equally increased attached gingiva; (3) pocket reduction achieved with osseous recontouring was maintained over 6 months, pockets recurring after open curettage; (4) open curettage did not induce bone regeneration; (5) osseous recontouring did not result in irreversible tooth mobility; (6) osseous recontouring resulted in a net loss of attachment; open curettage producing a net gain, especially in deeper pockets; and (7) both procedures improved periodontal health.

Alveoloplasty↗

Five year evaluation of the excisional new attachment procedure.

Patients treated with the Excisional New Attachment Procedure were evaluated 5 years or more following the procedure. They had received maintenance therapy about four times a year. Clinical measurements of free gingival margin height, pocket depth, and width of keratinized gingiva were made from the cemento-enamel junction in 56 surgical sites and compared to the same parameters recorded presurgically and at 1 and 3 years postsurgically. Probeable depths increased slightly and the amount of previously gained new attachment decreased slightly at each postoperative evaluation period. Only isolated sites were the same as or worse than pretreatment levels. An overall mean net gain in clinical attachment of 1.5 mm was found at 5 years after treatment, and probeable depths approached 3.0 mm at this time. Both of these 5 year findings were statistically and clinically significant improvements over preoperative measurements.

Epithelial Attachment↗