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PubMed1981

Effect of the alpha subunit of equine LH on the FSH induced cAMP production in rat seminiferous tubule cells.

Previous observations from our laboratory have shown that equine LH can suppress the FSH induced cyclic AMP production in rat seminiferous tubule cells in vitro. The present investigation was carried out to determine the effect of various subunits in this system. The ratios (w/w) of subunits to equine FSH tested was 1:3, 3:1 and 30:1 with a standard dose of 0.3 microgram of the FSH. It was noted that equine LH-beta was not effective up to a 10 microgram concentration in inhibiting the cyclic AMP production induced by equine FSH. Under these conditions, equine LH-alpha suppressed the FSH activity in a dose dependent manner. However, alpha subunits derived from several other species of LH were without any effect on FSH action. Histidine modified derivative of equine LH and its alpha subunit, both of which lack biological activity in the rat Leydig cell assay for LH, were found to be inactive as inhibitors of the equine FSH response. Thus, these results show that the suppressive effect of equine LH on FSH action in the rat seminiferous tubule is a function of the equine LH alpha subunit.

Animals↗
PubMed1984

Abnormality of testicular FSH receptors in infertile men.

To ascertain whether abnormalities in testicular binding of follicle stimulating hormone (FSH) are related with spermatogenic impairment found in idiopathic male infertility, we measured FSH receptors in testicular tissues obtained by biopsy from 37 infertile men. Since there had been few reports on the characterization of human testicular FSH receptor, we first established conditions for FSH receptor assay in small amounts of human testicular tissues taken by biopsy. Thirty-seven infertile men were divided into 3 groups by histological grading using Johnsen's score count, and testicular FSH receptors among these groups were compared. Although 6 of 12 infertile men with low Johnsen's score count and 3 of 9 men with middle score count had no obvious FSH binding sites with high affinity, all 16 men with high score count had high affinity FSH binding sites. The present findings demonstrate that the decrease in testicular high affinity binding sites for FSH is related with the degree of spermatogenic impairment found in idiopathic male infertility.

Adult↗
PubMed1995

FSH receptor gene expression during ovarian follicle development in sheep.

A key question in elucidating the role of FSH in ovarian function is to determine when during follicular growth the FSH receptor first appears. The aim of this study was to examine the site and time of FSH receptor gene expression during early follicular growth. This study was carried out on ovaries of adult sheep during the luteal and prostaglandin-induced follicular phase of the oestrous cycle and also on ovaries of fetal sheep at 90, 100, 120 and 135 days of gestation (term = day 147). Using reverse transcription-PCR and a set of PCR primers spanning exons 8/9/10, two partial FSH receptor cDNAs (500 and 310 bp) were isolated from adult sheep ovary. It was shown by sequencing that exon 8 was deleted in the 310 bp cDNA, implying that this was part of an alternatively spliced FSH receptor transcript. Using RNA in situ hybridisation on ovaries of adult sheep, FSH receptor mRNA was observed in granulosa cells of early preantral follicles with one to two cell layers and it was seen that gene expression continued throughout folliculogenesis into advanced stages of atresia. Moreover, in the fetus, FSH receptor gene expression was detected in follicles with two or more layers of granulosa cells in ovaries taken at 100, 120 and 135 days of gestation. These results suggest that the FSH receptor gene is expressed after the granulosa cells of a follicle have begun to divide but not during the earliest stages of follicle growth, namely the transformation of a primordial follicle to a primary follicle.

Animals↗
PubMed1987

Effects of cycloheximide during the periovulatory period on ovarian follicular FSH, hCG, and prolactin receptors and on follicular maturation in the hamster.

Hamsters were injected sc at 1400 hr on proestrus with either 4 mg cycloheximide (which blocks ovulation but only transiently affects ovarian protein synthesis) or saline and killed at 2-hr intervals until 0400 hr on estrus. After cycloheximide, the first surge of FSH (at 1600 hr) was half the normal value and the second surge of FSH (beginning at 2200 hr) was eliminated. Control follicles at 1400 hr had approximately the same number of FSH and hCG receptors with about one-third as many PRL receptors. Down regulation of FSH and hCG receptors for control follicles occurred by 2400 hr while PRL receptors dropped abruptly 4 hr earlier. Compared to the 1400-hr control values, the maximal loss of FSH, LH, and PRL receptors was 40, 45, and 85%, respectively. Although cycloheximide tended to slightly delay the loss of FSH receptors at 2000-2200 hr it did not prevent the ultimate fall in FSH and hCG receptors; the loss of PRL receptors was accelerated by 4 hr. Cycloheximide prevented or delayed follicular growth, resumption of meiosis, and cumulus expansion. The altered proestrous profile of steroids after cycloheximide (prolonged follicular estradiol and reduced progesterone) is therefore not associated with drastic alterations in the number of FSH and hCG binding sites. On the other hand, PRL receptors represent fast turnover protein(s).

Animals↗
PubMed2001

Ovarian function and FSH receptor characteristics during canine anoestrus.

Ovaries of bitches are relatively inactive during anoestrus despite apparently adequate circulating FSH concentrations. Alternative FSH receptor (FSH-R) transcripts in bitches might hinder the follicular response to gonadotrophins, which may account for anoestrus. The expression of the full length FSH-R and novel isoforms in bitches was examined using in situ hybridization and RT-PCR analysis. Various PCR primers to the FSH-R were used and its expression was assessed in ovarian tissue at different stages of the oestrous cycle. RT-PCR amplification of the extracellular domain (exon 1-10) was generally successful, indicating that cFSH-R expression (> 90%) occurs throughout the oestrous cycle. Two FSH-R isoforms were sequenced, but there were no clear differences in the pattern of expression between anoestrus and other stages of the oestrous cycle, except that isoform expression was less frequent (30% occurrence) in prepubertal bitches. Data from in situ hybridization showed clear expression of the FSH-R in secondary and antral follicles, and corpora lutea. It was concluded that there is no evidence of a change in the expression of the FSH-R specific to anoestrus.

Amino Acid Sequence↗
PubMed2001

Mutation screening of the FSH receptor gene in infertile men.

Follicle stimulating hormone (FSH) is important for controlling spermatogenesis through binding with its receptor. However, little information is available on mutations of the FSH and its receptor gene in infertile men. To study the genetic defects, which caused problems in spermatogenesis, we screened the point mutations of the FSH receptor gene in infertile men with high serum FSH concentrations. Seventy male infertile patients with high FHS levels (> 12 mIU/ml) were screened for mutations in each of the 10 exons of the FSH receptor gene, using genomic DNA PCR and a single-strand conformation polymorphism (SSCP) analysis. From this study, three shifted bands were detected by SSCP. The first shifted band was found in the PCR product of exon 4, including the exon-intron boundary sequence in only one patient. The sequence analysis revealed a nucleotide A to T substitution in intron 3 (IVS3-4A-->T). The second shifted band was detected in exon 10 with high frequency (33%). A nucleotide A to G substitution was found at the position of the 994th nucleotide, predicting a Thr to Ala substitution at the position of the 307th amino acid (Thr307Ala). The third shifted band in the 3' region of exon 10 was detected frequently in infertile patient and normal groups. It was tightly linked to the Thr307Ala variant. Thus, all of the abnormalities represent neutral polymorphisms, and not pathological mutations of the FSH receptor gene. In conclusion, we did not confirm that the genomic mutation of the FSH receptor is a major genetic cause in Korean infertile patients with high FSH levels.

Follicle Stimulating Hormone↗
PubMed1983

Immunocytochemical staining of isolated rat Sertoli cells for anti-FSH beta.

Sertoli cells are a primary target for the action of follicle-stimulating hormone (FSH) in the testis. The purpose of this investigation was to verify ultrastructurally that FSH binds to receptors on the plasma membrane of isolated rat Sertoli cells. A relatively pure aliquot of Sertoli cells was obtained by first dissociating testicular tissue from immature rats with collagenase and then centrifuging the suspension in Percoll density gradients. Pre-embedding staining with the peroxidase anti-peroxidase (PAP) complex technique using anti-FSH beta as the primary antiserum localized endogenous receptor-bound FSH on the plasma membrane of isolated Sertoli cells. Staining was considered to be specific since membranes of Sertoli cells derived from hypophysectomized rats were not stained when subjected to the same procedure. Cytoplasmic vesicles in Sertoli cells from experimental, control, and hypophysectomized groups also stained with PAP. Staining of these structures appeared to be specific since it was obliterated by preabsorption of anti-FSH beta with FSH. Preabsorption with luteinizing hormone (LH) did not affect the staining of cytoplasmic vesicles. The results of this investigation provide the first evidence for ultrastructural localization of specific binding sites for anti-FSH beta on the cell membrane of isolated Sertoli cells using an unlabeled antibody technique, and they further support the contention that Sertoli cells are a primary target for the action of FSH.

Animals↗
PubMed1990

Cellular regulation of basal and FSH-stimulated cyclic AMP production in irradiated rat testes.

Basal and follicle-stimulating hormone (FSH)-stimulated cyclic AMP (cAMP) productions by seminiferous tubular segments from irradiated adult rats were investigated at defined stages of the epithelial cycle when specific spermatogenic cells were low in number. Seven days post-irradiation, depletion of spermatogonia did not influence the basal cAMP production, but FSH response increased in stages II-VIII. Seventeen days post-irradiation when spermatocytes were low in number, there was a small increase in basal cAMP level in stages VII-VIII and FSH-stimulated cAMP production increased in stages VII-XII and XIII-I. At 38 days when pachytene spermatocytes and round spermatids (steps 1-6) were low in number, a decreased basal cAMP production was measured in stages II-VI and IX-XII. FSH-stimulated cAMP output increased in stages VII-XII but decreased in stages II-VI. At 52 days when all spermatids were low in number, basal cAMP levels decreased in all stages of the cycle, whereas FSH response was elevated only in stages VII-XII. All spermatogenic cell types seem to have an effect on cAMP production by the seminiferous tubule in a stage-specific fashion. Germ cells appear to regulate Sertoli cell FSH response in a paracrine way, and a part of cAMP may originate from spermatids stimulated by an unknown FSH-dependent Sertoli cell factor. The FSH-dependent functions may control such phenomena as spermatogonial proliferation, final maturation of spermatids, and onset of meiosis.

Animals↗
PubMedDate not supplied

Clinical studies in an adult male patient with "isolated follicle stimulating hormone (FSH) deficiency".

Previous reports concerning isolated follicle stimulating hormone (FSH) deficiency and its possible pathogenesis have been conflicting. Both "normal" and "abnormal" FSH response to luteinizing hormone releasing hormone (LHRH) infusion have been described. We studied a 22-year-old man with normal basal serum testosterone and luteinizing hormone (LH) levels but undetectable levels of serum FSH. His serum LH titers showed one secretory spike during a 40-hour sampling at 20-minute intervals, whereas his serum FSH titers remained undetectable (less than 0.4 IU/l). Infusion of LHRH, 0.2 microgram/minute for 4 hours, induced the expected rise in the serum LH levels, but serum FSH levels remained low and only at one point reached 0.9 IU/l (normal adult male basal range 0.9-10.3 IU/l). The patient received LHRH, 100 micrograms/day, for three days. A second LHRH infusion, 0.2 microgram/minute for 4 hours, induced a normal rise in both the serum LH and FSH titers. The serum sex steroid binding globulin level was 10.3 ng DHT bound/ml (normal adult male level 8.0 +/- 0.3 ng DHT bound/ml). Presence of circulating auto-antibodies to the serum FSH was excluded by determining the binding of [125I] FSH with the patient's serum and comparing it with sera obtained from two normal male adult volunteers. Pituitary function tests were otherwise intact. Presence of a pituitary tumor was excluded by computerized axial tomography and x-ray studies of the pituitary fossa and normal visual fields. Clinically, the patient demonstrated cryptorchidism, hypospadias, surgically repaired omphalocele, and bilateral hearing loss.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1991

Prostate--an extrapituitary source of follicle-stimulating hormone (FSH): occurrence, localization, and de novo biosynthesis and its hormonal modulation in primates and rodents.

A comparative study on the immunocytochemical localization, de novo biosynthesis, and hormonal modulation of follicle-stimulating hormone (FSH) was carried out in the prostates of man, monkey, dog, guinea pig, hamster, rat, and mouse. FSH was localized in the cytoplasm of the prostatic epithelial cells. In some specimens, staining was also observed in the nucleus. Both pituitary as well as prostatic FSH were coeluted on a Sephadex G-100 column and high-performance liquid chromatography (HPLC) indicating physicochemical similarities of FSH in both the tissues. Surprisingly, the modulation of pituitary and prostatic FSH by inhibin and its related peptides were comparable. The intensity and grandularity of FSH staining was stronger in the case of benign prostatic hyperplasia as compared with normal prostatic specimens. In view of the well-known effects of FSH on the cellular growth, differentiation, and function of gonadal tissues, a similar role for FSH in pathophysiology of prostate is postulated.

Adult↗
PubMed1983

Immunocytochemical localization of LH, FSH and TSH in the fetal porcine pituitary.

Antibodies (i) against porcine LH (A-pLH) and porcine FSH (A-pFSH), and (ii) against their subunits porcine LH beta, porcine FSH beta, porcine TSH beta, and rat LH alpha were used in the present study. The aim of this study was (1) to detect by the use of the immunoperoxidase technique the earliest stages of immunoreactive gonadotropic and thyrotropic cells, and (2) to study the development of the synthesis and the storage of LH, FSH and TSH in the fetal porcine anterior pituitary. The following results were obtained: (1) LH, FSH and TSH appeared between days 40 and 45 of fetal life; immunoreactive LH was found on day 40, immunoreactive TSH and FSH at day 45; thus, the first gonadotropic cells containing both LH and FSH were observed at day 45 (LH/FSH cells). (2) The alpha-subunit appeared to be present earlier than the beta-subunits or complete gonadotropic and thyrotropic hormones. (3) LH became manifest earlier than FSH. These findings showed clearly the onset of the synthesis of LH and FSH at days 40 to 45 of fetal life, and a progress until day 75, being complete at day 80 and resulting in a population of LH/FSH cells. At about day 90, the most predominant type of gonadotropic cell was the LH/FSH cell; however, some cells appeared to be exclusively LH cells.

Animals↗
PubMed1994

A morphometric analysis of the subcellular distribution of LH beta and FSH beta in secretory granules in the pituitary gonadotrophs of the frog (Rana japonica).

Immunohistochemical localization of lutropin beta (LH beta) and follitropin beta (FSH beta) in the pituitary gland of the frog Rana japonica was studied by the peroxidase-anti-peroxidase method and the two-face, double-labeling method with different-sized gold particles at the light- and electron-microscopic levels, respectively, using monoclonal antibodies against bullfrog LH beta and FSH beta. Light-microscopic immunohistochemistry indicated that approximately 66.0% of all the gonadotrophs in the pituitary contained both LH beta and FSH beta, whereas 33.4% of gonadotrophs contained only LH beta, and 0.6% contained only FSH beta. The staining intensity of LH beta and FSH beta varied from cell to cell. The gonadotrophs were classified into four types (Types I-IV) in terms of their ultrastructural and immunolabeling characteristics. Moreover, several secretory granule types were recognized according to differences in their shape and electron density. In all the cell types, both LH beta and FSH beta were often seen in the same secretory granules, but the proportion of granules bearing both hormones ranged from 5.5% in Type I to 32.7% in Type IV. Most secretory granules in Types I and II were immunolabeled with LH beta alone, whereas a small number of granules were immunolabeled with FSH beta alone. More immunolabeled FSH beta granules were present in Types III and IV than in Types I and II.

Animals↗
PubMed1989

Influence of thyroidectomy on serum and pituitary FSH in control and orchidectomized rats.

The influence of hypothyroidism on both pituitary and serum FSH has been studied in male rats thyroidectomized in early stages of development. To know whether FSH alterations are produced by changes of the gonadal feed-back system or by a direct effect of thyroid hormones upon pituitary synthesis, estradiol, testosterone and testicular weight were measured. The result showed a decrease of biological and immunological activity of pituitary FSH in thyroidectomized rats. Serum FSH levels were also low. The deficiency of FSH production cannot be attributed to an alteration of the gonadal feed-back systems, since (1) in thyroidectomized rats, estradiol and testosterone were lower than in the controls, (2) signs of tubular atrophy were found in the testes and hence it could be expected that inhibin is low, and (3) in the absence of gonadal factors (induced by orchidectomy) thyroidectomized rats also showed a decrease of pituitary FSH. The present data suggest that the decrease of FSH can be attributed to a direct effect of thyroid hormones on pituitary FSH production.

Animals↗
PubMed1994

Basal follicle stimulating hormone (FSH) predicts response to controlled ovarian hyperstimulation (COH)-intrauterine insemination (IUI) therapy.

PURPOSE: This work investigates the relationship of basal follicle stimulating hormone (FSH) measurements and age to ovarian responsiveness and pregnancy occurrence following controlled ovarian hyperstimulation and intrauterine insemination (COH-IUI). METHOD: Basal FSH was measured retrospectively in sera from infertility patients obtained on cycle day 2, 3, or 4 of a COH-IUI treatment cycle. Basal FSH was then correlated with COH response parameters and treatment outcome in ovulatory (n = 98) and anovulatory (n = 33) patients. Clinical data were collected from retrospective chart review. In the ovulatory group, increasing basal FSH was associated with fewer follicles (P = 0.01) and lower peak estradiol (P = 0.0001). RESULTS: No age related effects were detected in the anovulatory group. Also, there were no pregnancies in this group when basal FSH was > 22.1 mIU/ml. Increasing age in the ovulatory group was associated with fewer follicles (P = 0.0001), lower peak estradiol (P = 0.0001), and fewer pregnancies (P = 0.04). In the anovulatory group, basal FSH did not correlate with follicle numbers or peak estradiol, although more ampoules of hMG were used for stimulation (P = 0.03). CONCLUSIONS: Increasing basal FSH and patient age both correlated inversely with ovarian responsiveness to COH in ovulatory patients. Basal FSH can be used in clinically to identify patients undergoing COH who are unlikely to respond to superovulatory drugs and unlikely to become pregnant.

Adult↗
PubMed1988

Characterization of follicular fluid stimulatory factor upon FSH-induced granulosa cell differentiation.

The modulatory role of follicular fluid (FF) upon gonadotropin-induced granulosa cell (GC) differentiation has been previously demonstrated. In the present study, the stimulatory factor of large-antral FF (LFF) was partially characterized. Addition of ovine FSH to porcine GC culture increased 125I-hCG specific binding about 6-fold and progesterone (P4) secretion 15-fold. The FSH-induced 125I-hCG specific binding was increased 1.9-fold by LFF and decreased 7.7-fold in the presence of small-antral FF (SFF). It is suggested that SFF may contain an inhibitory factor which is overcome by a stimulatory factor accumulated in FF with follicular growth. An initial purification of LFF stimulatory factor was achieved by two-step ethanol elution. The obtained LFF purified fraction increased the FSH-induced 125I-hCG specific binding 1.5-fold and P4 secretion 4.3-fold compared to GC cultured with FSH + LFF. Addition of LH alone increased the P4 secretion 2.3-, 3.4-, and 6 fold in GC precultured with FSH alone, FSH + neat LFF, and FSH + LFF purified fraction, respectively, as compared to GC precultured in plain medium. Therefore, the LFF stimulatory increase of 125I-hCG specific binding may represent an increase of functional LH receptors. Freezing and thawing of LFF 4 times, exposing LFF to urea for 24 h, heating at 60 C for 20 min, and tryptic digestion each abolished the LFF stimulatory effect upon the FSH-dependent increase of 125I-hCG specific binding and P4 secretion. Thus, the LFF stimulatory activity could be attributed to one or more proteins.

Animals↗
PubMed2006

The relation of germ cells per tubule in testes, serum inhibin B and FSH in cryptorchid boys.

At bilateral orchiopexy bilateral biopsies may be indicated to determine fertility potential. It is currently unknown if the serum inhibin B levels at time of orchiopexy reflect the testicular status of the bilaterally cryptorchid child. The aim of this study was to relate the results of inhibin B and FSH measurements with testicular biopsy parameters in bilateral cryptorchid boys. Included were 25 boys with bilateral cryptorchidism, median 2.5 years (9 months to 5.5 years) at surgery for bilateral cryptorchidism with simultaneous testicular biopsies, and blood sample for inhibin B and FSH. The number of spermatogonia and gonocytes was measured in 100 tubular transverse sections, the S/T and the mean-S/T of the patient was found, and expressed as percent of lowest normal value. Inhibin B and FSH were measured and related to age-specific values. Forty percent (10/25) of the patients had very low mean-S/T (mean-S/T<10% of lowest normal-value). Inhibin B was decreased in 24% (6/25) of the patients, all with decreased mean-S/T, predominantly with a mean-S/T<10% of lowest normal-value (p<0.05). There was a negative correlation between inhibin B and FSH (p<0.05). In cases of mean-S/T<10% of lowest normal-value and decreased inhibin B, we found increased FSH in 9% (2/22) of the patients and hypergonadotropic hypogonadism was suspected. Low FSH was found in 5% (1/22) of the patients and hypogonadotropic hypogonadism was suspected. Low inhibin B predicts a serious condition in respect of infertility. Low FSH and inhibin B indicates examination for hypogonadotropic hypogonadism. In bilateral cryptorchid boys inhibin B levels correlated negatively to FSH.

Child, Preschool↗
PubMed2006

Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation.

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform Fundulus heteroclitus raised against the conservative region of the teleost betaFSH and the betaLH subunits. The estimated molecular weights were 24 kDa for LH and 19 and 15 kDa for FSH. In the adult pituitary, both cell types were distributed along mid and ventral zones of the proximal pars distalis (PPD, mid-immunoreactive cells), and along the ventral and dorsal external border of the pars intermedia (PI, high-immunoreactive cells). Double IHC showed that FSH and LH are mainly expressed in different pituitary cells. FSH cells were detected in the pituitary around day 21 after hatching (ah) (prior to sex differentiation), while LH cells were detected by day 60 ah (during the sexual differentiation period). A correlation between gonadal sex differentiation and FSH was demonstrated in a 15 days organ culture system. FSH and LH neurons were localized in the nucleus lateralis tuberis and their fibers project through the ventral hypothalamus, preoptic area and neurohypophysis. FSH neurons differentiated on day 21 ah, while LH neurons appeared on day 15 ah. In the ovary, the immunoreactivity for both FSH and LH was restricted to the cytoplasm of previtellogenic and early vitellogenic oocytes.

Age Factors↗
PubMed1996

Differential effects of the N-methyl-D-aspartate and non-N-methyl-D-aspartate receptors of the excitatory amino acids system on LH and FSH secretion. Its effects on the hypothalamic luteinizing hormone releasing hormone during maturation in male rats.

The present experiments describe the effect of NMDA and kainate agonists of the NMDA and non-NMDA subtype of receptors respectively of the excitatory amino acids (EAAs) system in prepubertal (16 days of age) and peripubertal (30-day-old rats) male rats on the in vitro hypothalamic release of GnRH, and on the in vivo LH and FSH levels as well as the effect of testosterone on these effects. The addition of NMDA or kainate to the medium containing APOA-MBH areas significantly increased (P < 0.01) the GnRH release as compared with the respective controls. The increase in GnRH release observed with kainate was significantly higher (P < 0.01) than those observed with NMDA. NMDA administration increased significantly (P < 0.01) serum LH levels at both ages of sexual maturation while no effect was observed by kainate administration. MK 801, an antagonist of NMDA neurotransmission, and testosterone abolished the LH release response to NMDA. Contrary to that observed on LH, while NMDA did not modify serum FSH concentrations a significant increase (P < 0.01) was observed with kainate administration in prepubertal and peripubertal rats on this pituitary hormone, and CNQX, an antagonist of non-NMDA neurotransmission, and testosterone administrations blocked this FSH release effect of kainate. The NMDA and kainate release effect on LH and FSH respectively was significantly higher in prepubertal than in peripubertal rats. At both ages NMDA released more LH than kainate FSH. In conclusion, our experiments demonstrated that both subtypes of glutamate receptors NMDA and non-NMDA subtypes of EAAs increased GnRH release by APOA-MBH in vitro during sexual maturation. Nevertheless, while NMDA administration only increased serum LH levels, kainate showed only an effect on increasing FSH concentrations. These differential effects of NMDA and non-NMDA subtypes of EAA receptors on LH and FSH could probably explain some aspects of the differential modifications of LH and FSH observed in different physiological circumstances.

Animals↗