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[The value of visual judgements of the calcium content of the skeleton (author's transl)].

The mineral content in 113 forearm bones were evaluated visually and compared with the results of measurements of the cortex and of iodine 125 photon absorption measurements. There was a significant correlation between measurements of cortical width and direct measurements of mineral content. In general, visual grading into four groups was reasonably accurate, but in individual cases there were considerable errors. Of 11 cases with a mineral loss of more than 30%, only one was regarded as normal. A particular difficulty in the visual evaluation was the great physiological variation of mineral content related to age and sex; this must be taken into account.

Adult↗

Is there a future for single photon emission tomography?

Emission tomography has the potential to be developed into a clinically useful technique. Its clinical applications, however, will be limited and not in the main stream of space occupying disease detection but in the definition of quantitative information particularly relevant in those disease processes where the metabolic component is the most important one. Special purpose designed instrumentation may prove to be the best of several possible compromises.

Elementary Particles↗

[Digital-subtracted magnetic resonance (DSMR). A new procedure for improved imaging of vascular processes].

Using varying echo-sequences, NMR studies of flow in vessels shows varying signal relationships which are caused by intermittent re-focussing of the elementary magnetic particles. By means of digital subtraction of the tomograms, this effect can be clearly shown. It has been demonstrated that this particular procedure can be used for improved visualisation of vessels and of abnormal vascular processes (thrombosis, tumours, aneurysms etc.).

Aged↗

Temperature profile for glacial ice at the South Pole: implications for life in a nearby subglacial lake.

Airborne radar has detected approximately 100 lakes under the Antarctic ice cap, the largest of which is Lake Vostok. International planning is underway to search in Lake Vostok for microbial life that may have evolved in isolation from surface life for millions of years. It is thought, however, that the lakes may be hydraulically interconnected. If so, unsterile drilling would contaminate not just one but many of them. Here we report measurements of temperature vs. depth down to 2,345 m in ice at the South Pole, within 10 km from a subglacial lake seen by airborne radar profiling. We infer a temperature at the 2,810-m deep base of the South Pole ice and at the lake of -9 degrees C, which is 7 degrees C below the pressure-induced melting temperature of freshwater ice. To produce the strong radar signal, the frozen lake must consist of a mix of sediment and ice in a flat bed, formed before permanent Antarctic glaciation. It may, like Siberian and Antarctic permafrost, be rich in microbial life. Because of its hydraulic isolation, proximity to South Pole Station infrastructure, and analog to a Martian polar cap, it is an ideal place to test a sterile drill before risking contamination of Lake Vostok. From the semiempirical expression for strain rate vs. shear stress, we estimate shear vs. depth and show that the IceCube neutrino observatory will be able to map the three-dimensional ice-flow field within a larger volume (0.5 km(3)) and at lower temperatures (-20 degrees C to -35 degrees C) than has heretofore been possible.

Antarctic Regions↗

Detection of DNA synthesis in intact organisms with positron-emitting (methyl- 11 C)thymidine.

(11)CO(2) produced in the Brookhaven 152-cm cyclotron was converted to formaldehyde, which in turn was used for the enzymatic conversion of deoxyuridine-5'-phosphate to [(11)C]thymidylate. Enzymatic treatment of the nucleotide with alkaline phosphatase gave [(11)C]thymidine.The preparation of [(11)C]thymidine from cyclotron-generated (11)CO(2) required 110 min (about 5 half-lives): 35 min for the synthesis of H(11)CHO, 25 min for the enzymatic conversion to [(11)C]thymidylate, 20 min for column chromatography, 5 min for phosphatase treatment, 10 min for evaporation, 2 min for filtration through an anion-exchange resin, and 13 min for miscellaneous manipulations.Positron-emitting [(11)C]thymidine and [(11)C]thymidylate were used for in vivo tracer studies of DNA synthesis in mice for periods of up to 3 hr. Findings with carbon-11 were consistent with earlier studies in which carbon-14 and tritium-labeled thymidine were used. For example, 3 hr after injection of [(11)C]thymidine, spleen DNA was labeled to a much greater extent than was liver DNA.

Alkaline Phosphatase↗

Reciprocity or near-reciprocity of highly coupled enzymatic processes at the multidimensional inflection point.

It has recently been demonstrated that coupled enzymatic processes may possess, for a particular choice of the state variables, multidimensional inflection points in thermodynamic force-flow space. The conditions for reciprocity in the linear region near such a reference state, which may be far from equilibrium, are of considerable interest. It is shown by examining the associated Hill diagrams that all cycles in which a given pair of forces act contribute a corresponding pair of symmetrical terms to the Jacobian matrix characterizing perturbations about this stationary state. To the extent that these cycles dominate--i.e., to the extent that the system is highly coupled--reciprocity or near-reciprocity will be obeyed. This would be expected to be the case in most biological systems.

Adenosine Triphosphate↗

String theory.

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Elementary Particles↗

An investigation of the characteristics of a negative pion beam by means of induced chromosome aberration in human peripheral blood lymphocytes.

The depth dose profiles of unstable chromosome aberrations were determined by irradiating blood samples at various depths in a plastic phantom exposed to a beam of negative pi mesons (pions). The pions were derived from an experimental beam line from the Nimrod 7 GeV proton synchrotron. With a momentum of 160 MeV/c and a spread of 15 per cent a Bragg profile was produced peaking at 13-9 cm. An ionization peak to plateau ratio of 1-6 was obtained and for cytogenetic damage this was increased to 2-9. Dicentric and acentric dose-response data from peak and plateau positions were fitted to Y=alphaD+betaD-2 models for comparison with data obtained from 60-Co gamma-rays and fission neutrons. With respect to 150 rads gamma-radiation, the r.b.e. values for the pion peak were 2-1 for dicentrics and 2-3 for acentrics. In the plateau the value for both aberrations was 1-5. At pion doses likely to be used as daily radiotherapy fractions, the aberrations predominantly resulted from single-track events, indicating little likelihood of interfraction recovery, particularly in the peak position. It is concluded that the biological damage characteristics of pion beams, as determined from a cytogenetic end-point, offer several features attractive to the radiotherapist.

Chromosome Aberrations↗

Negative pion irradiation of mammalian cells. 1. Survival characteristics of monolayers and spheroids of Chinese hamster lung cells.

Monolayers and spheroids of Chinese hamster cells (V79) were subjected to negative pion irradiation under aerobic conditions. R.b.e. values in the pion peak of 1-8 and 1-5 were obtained for monolayers and spheroids, respectively, whereas the r.b.e. for the plateau was found to be slightly higher than 1. In addition, it was observed that the higher resistance of the V79 spheroid cells than the monolayers to gamma-irradiation is not diminished in the pion peak, suggesting that the underlying phenomenon of intercellular communication influences cell survival even after high-LET irradiation.

Cell Line↗

Abscopal effect of irradiation on haemopoietic stem cells of shielded bone marrow--role of migration.

Immediately after partial-body irradiation, the number of colony-forming units (CFU) of the protected marrow decreases. This decrease seems to be dose-dependent and cannot be completely explained by migration for the following reasons: there is no evidence of CFU entry to irradiated territories and the number of circulating CFU does not increase. A more plausible explanation for the phenomena is an accelerated differentiation during the first 15 min after irradiation. The migration of CFU to the spleen is observed between 15 min and 3 hours after irradiation, after which it ceases. The absence of migration 3 hours after irradiation may be due to one of the two possibilities: (1) a depletion of the mobile pool of CFU if one admits that such a pool is responsible for migration in the organism; (2) a depletion of a temporarily increased stimulus due to irradiation.

Animals↗