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Volumetric evaluation of therapy response in patients with lung metastases. Preliminary results with a computer system (CAD) and comparison with unidimensional measurements.

PURPOSE: The aim of this study was to assess the efficacy of a Computer-Aided Detection (CAD) system in the identification of lung metastases and to compare the volumetric CAD measurements with unidimensional observer measurements in the evaluation of treatment response in oncology patients. MATERIALS AND METHODS: Two observers (A and B) evaluated nine patients undergoing lung computed tomography (CT) just before and immediately after treatment with chemotherapy. Multislice CT scans were performed before and after the injection of contrast material with a high-resolution protocol (collimation 4x1 mm, 100 mAs, 120 kV). Response Evaluation Criteria in Solid Tumours (RECIST) criteria were used to consider the disease as stable, increased or decreased. Subsequently, target lesions (most significant lesions identified before and after chemotherapy) were evaluated with a computerised system (CAD) to establish volumetric measurements. Observers' unidimensional measurements and CAD volumetric measurements were analysed for comparison. RESULTS: Twenty-four nodules (diameter: 5-18 mm in the first study and 4-20 mm in the follow-up study) were included. Observers agreed in the assessment of therapy response in 21 nodules: eight were considered to have increased in size, and 13 were judged stable. Observer and CAD measurements disagreed in three nodules: two were considered stable by radiologists and increased by CAD; one was considered increased by radiologists and stable by CAD.As regards patient response, radiologists disagreed in two cases. CAD and observers did not agree in one case. CONCLUSIONS: Our preliminary data suggest that volumetric measurements can modify the diagnostic and therapeutic evaluation of oncology patients under chemotherapy.CAD volumetric measurements allow an easy and objective evaluation, reducing interobserver variability in the evaluation of chemotherapy response.

Adult↗

Multi-platforms medical computer systems integration.

Presently, software architects face the challenge of integrating and linking different application software packages built on different computer platforms. Often they inherit various systems, ranging from mainframe to PDA-based applications. The first stage before programming different interfaces is to identify the means of communications most suitable for particular systems. The experience of the Mount Sinai Medical Center in New York, which combines a hospital, a healthcare network, a research center and a medical school, illustrates common problems faced by a number of medical institutions. This paper will discuss the various options, including the Internet, software architects have, and how they can use them during the development of an infrastructure for health care systems.

Academic Medical Centers↗

A sample computer system for physiological data acquisition and analysis.

This report outlines a sample configuration of a system which records, stores and analyses, graphically and statistically, neurophysiological and cardiovascular recordings during an experiment. The system is composed of sensitive physiological amplifiers, an analog to digital signal conversion board, scientific software, a 80286-based computer with 640 Kb of RAM, and a laser printer. Each component of the system is described along with the specific task(s) it performs.

Analog-Digital Conversion↗

Computer systems for the prediction of xenobiotic metabolism.

The aim of pharmaceutical research and development is to ensure a continuing pipeline of new chemical entities (NCEs) displaying high therapeutic efficacy with few or no side effects. Failure of promising lead candidates late in the drug discovery processes is regarded as commercially unacceptable in today's increasingly competitive business environment. An inappropriate ADME/Toxicity profile in humans is the major cause of failure of lead candidates in late clinical stages of drug development. Combinatorial chemistry techniques coupled with high throughput screening protocols means that pharmaceutical companies are now dealing with an unprecedented number of NCEs on an annual basis. As a consequence, screening for undesirable ADME/Toxicity properties in the early stages of drug development, preferably pre-synthesis, is now considered the essential paradigm. In silico assessment of NCEs is rapidly emerging as the next wave of technology for early ADME/Toxicity prediction. In this review, we discuss the major commercially available products for the assessing the potential metabolic activity of xenobiotic substances in mammalian systems.

Animals↗

Restriction fragment length polymorphism: molecular weight analysis and calculation with a scanner-based computer system.

For an exact and reproducible molecular weight calculation, an essential requirement in all applications of DNA profiling, we present a new system, called RFLP-MAC (restriction fragment length polymorphism-molecular weight analysis and calculation). The system is composed of a sensitive digital gray-scale scanner unit and an Apple Macintosh computer supported by a powerful combination of a shareware and self-developed software package. Molecular weights are calculated by profiling the optical density and band particle processing parameters to identify the unknown DNA fragments. Mathematical models were used to interpolate the transverse and horizontal migration directions of the molecular weight standards, insuring consistent results from gel to gel. All data are stored in a relational multi-user database system. Descriptive statistics are performed and allele frequencies are analyzed. Procedures for image-analysis, molecular weight calculation, statistical evaluations and database management were developed and run within the multitasking environment of 4th Dimension. Two sets of data, intragel and intergel measurements, are investigated in order to check the precision of the RFLP-MAC system. All these data are subjected to a standard analysis of variance resulting in a maximal 3 SD value of 0.005 (0.288%) for the intragel and of 0.091 (1.654%) for the intergel variation.

Computer Systems↗

GeneExpress: a computer system for description, analysis, and recognition of regulatory sequences in eukaryotic genome.

GeneExpress system has been designed to integrate description, analysis, and recognition of eukaryotic regulatory sequences. The system includes 5 basic units: (1) GeneNet contains an object-oriented database for accumulation of data on gene networks and signal transduction pathways and a Java-based viewer that allows an exploration and visualization of the GeneNet information; (2) Transcription Regulation combines the database on transcription regulatory regions of eukaryotic genes (TRRD) and TRRD Viewer; (3) Transcription Factor Binding Site Recognition contains a compilation of transcription factor binding sites (TFBSC) and programs for their analysis and recognition; (4) mRNA Translation is designed for analysis of structural and contextual features of mRNA 5'UTRs and prediction of their translation efficiency; and (5) ACTIVITY is the module for analysis and site activity prediction of a given nucleotide sequence. Integration of the databases in the GeneExpress is based on the Sequence Retrieval System (SRS) created in the European Bioinformatics Institute.

Artificial Intelligence↗