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Canine breeding management.

A prebreeding examination should be performed on animals of both sexes to ensure their optimal health at the time of breeding. At this time, the medical and reproductive histories should be reviewed to detect potential negative influences upon the reproductive tract. Serologic testing for canine brucellosis should be performed to detect and remove infected animals from the breeding program. Examination of the female reproductive tract may be limited to the caudal vagina, vestibule, and vulva. Defects that cause pain and reluctance to breed may be detected with thorough digital and visual inspection. The male reproductive tract can be examined along nearly its entire length without special techniques. Semen may be collected for initial evaluation of testicular function and fertility. Swelling of the vulva and a hemorrhagic vaginal discharge will alert the observant owner to the onset of proestrus. Vaginal cytology may be examined every few days after the observed onset of proestrus to determine the onset of estrus. Breeding should be performed by natural or artificial insemination every 2 to 4 days during estrus to achieve optimal conception rates. The bitch should not be exhibited or trained during the period of potential pregnancy. Unnecessary drugs should not be administered. A diagnosis of pregnancy may be made by abdominal palpation 28 to 30 days after the last breeding. Serum progesterone levels may be measured if pregnancy is not established to determine if ovulation occurred and if sufficient levels of progesterone are present to maintain pregnancy. Optimal breeding management techniques can often result in the maximum reproductive efficiency desired.

Animals↗

Relationships of hormonal patterns and fertility to occurrence of two or three waves of ovarian follicles, before and after breeding, in beef cows and heifers.

Ovarian follicular waves were characterized before and after breeding in 52 lactating beef cows and 16 heifers. Effects of two (2 W) or three (3 W) waves of follicular development and associated patterns of concentrations of steroids in jugular serum on pregnancy rate were examined. Animals were observed for oestrus (= day 0) twice daily and inseminated artificially at second oestrus. Follicular development was monitored by ultrasonography and jugular blood samples were collected simultaneously on alternate days from day 6 after first oestrus until ovulation after the second oestrus and from day 6 after second oestrus (insemination) until next ovulation or day 24 of pregnancy. Pregnancy was determined by ultrasonography at 25 days after insemination. From individual patterns of growth or regression of the largest follicles, more of the 59 animals with oestrous cycles of 17 to 25 days had 2 W (51, 86%) than 3 W (8, 14%) during the oestrous cycle before breeding (P < 0.01). Cycles averaged 1.1 days longer (P < 0.10) and corpora lutea regressed later (P < 0.01) in animals with 3 W vs. those with 2 W, but mean oestradiol between 7 and 2 days before second oestrus (3.7 +/- 0.3 pg ml-1) did not differ between cycles with 2 W or 3 W. Ovulatory follicles in animals with 2 W differed from those with 3 W (P < 0.05) in day of detection (12.3 +/- 0.3 vs 16.5 +/- 0.5), growth rate (1.0 +/- 0.1 vs 1.5 +/- 0.1 mm day-1), interval from detection to ovulation (9.3 +/- 0.3 vs 6.3 +/- 0.7 days) and duration of dominance (4.0 +/- 0.2 vs 2.1 +/- 0.6 days). Pregnancy rates, 82% in cows with 2 W and 100% in cows with 3 W, did not differ (P > 0.05). During the period equivalent to an oestrous cycle after breeding, 29 (49%) of the 59 animals had 2 W and 30 (51%) had 3 W. Fewer animals with 2 W than 3 W after breeding became pregnant (16/23, 70% vs 26/27, 96%; P < 0.05), but patterns of concentrations of progesterone on days 6 through 14 or mean oestradiol on day 14 (2.6 +/- 0.2 pg ml-1) did not differ. In conclusion, fewer animals had 3 W than 2 W before breeding and fertility did not differ. During the equivalent of one oestrous cycle after breeding, approximately equal numbers of animals had 2 W or 3 W and fertility was greater for animals with 3 W.

Animals↗

Ovarian function in ewes at the onset of the breeding season.

Transrectal ultrasonography of ovaries was performed each day, during the expected transition from anoestrus to the breeding season (mid-August to early October), in six Western white-faced cross-bred ewes, to record ovarian antral follicles > or = 3 mm in size and luteal structures. Jugular blood samples were collected daily for radioimmunoassay (RIA) of follicle-stimulating hormone (FSH), oestradiol and progesterone. The first ovulation of the breeding season was followed by the full-length oestrous cycle in all ewes studied. Prior to the ovulation, all ewes exhibited a distinct increase in circulating concentrations of progesterone, yet no corpora lutea (CL) were detected and luteinized unovulated follicles were detected in only three ewes. Secretion of FSH was not affected by the cessation of anoestrus and peaks of episodic FSH fluctuations were associated with the emergence of ovarian follicular waves (follicles growing from 3 to > or = 5 mm). During the 17 days prior to the first ovulation of the breeding season, there were no apparent changes in the pattern of emergence of follicular waves. Mean daily numbers of small antral follicles (not growing beyond 3 mm in diameter) declined (P < 0.05) after the first ovulation. The ovulation rate, maximal total and mean luteal volumes and maximal serum progesterone concentrations, but not mean diameters of ovulatory follicles, were ostensibly lower during the first oestrous cycle of the breeding season compared with the mid-breeding season of Western white-faced ewes. Oestradiol secretion by ovarian follicles appeared to be fully restored, compared with anoestrous ewes, but it was not synchronized with the growth of the largest antral follicles of waves until after the beginning of the first oestrous cycle. An increase in progesterone secretion preceding the first ovulation of the breeding season does not result, as previously suggested, from the ovulation of immature ovarian follicles and short-lived CL, but progesterone may be produced by luteinized unovulated follicles and/or interstitial tissue of unknown origin. This increase in serum concentrations of progesterone does not alter the pattern of follicular wave development, hence it seems to be important mainly for inducing oestrous behaviour, synchronizing it with the preovulatory surge of luteinizing hormone (LH), and preventing premature luteolysis during the ensuing luteal phase. Progesterone may also enhance ovarian follicular responsiveness to circulating gonadotropins through a local mechanism.

Animals↗

Individual assignment using microsatellite DNA reveals unambiguous breed identification in the domestic dog.

Modern individual clustering methods utilising hypervariable nuclear microsatellite DNA polymorphisms are being increasingly applied in the field of population genetics. This study explores the efficiency of the clustering methods in identifying the breeds of origin of 250 domestic dog (Canis familiaris) individuals based on 10 microsatellite loci. An allele sharing distance (DAS) matrix and the corresponding neighbour-joining tree of individuals revealed monophyletic assemblages that corresponded perfectly with the breeds of origin of the dogs. Individual assignment tests using a Bayesian statistical approach, an allele frequency based method, and a DCE genetic distance based method were all extremely powerful. Most strikingly, the Bayesian method provided 100% assignment success of individuals into their correct breeds of origin and 100% exclusion success of individuals from all alternate reference populations with a high level of statistical confidence (P < 0.0001). A Bayesian Markov Chain Monte Carlo clustering approach revealed clear distinction of individuals into groups according to their breeds of origin, with a near-zero level of 'genetic admixture' among breeds. The results demonstrate that an FST of 0.18, mean expected gene diversity of 0.6 across 10 loci, and approximately 50 individuals per reference population suffice to provide maximum individual assignment success in C. familiaris. This refutes the traditional view that DNA based dog breed identification is not feasible at the individual level of resolution.

Animals↗

Geographic distribution and frequency of a taurine Bos taurus and an indicine Bos indicus Y specific allele amongst sub-saharan African cattle breeds.

We report for the first time, and for the whole of sub-Saharan Africa, the geographical distribution and the frequency of an indicine and a taurine Y specific allele amongst African cattle breeds. A total of 984 males from 69 indigenous African populations from 22 countries were analysed at the microsatellite locus INRA 124. The taurine allele is probably the oldest one on the continent. However, the taurine and the indicine alleles were present in 291 males (30%), and 693 males (70%), respectively. More particularly, 96% of zebu males (n = 470), 50% of taurine males (n = 263), 29% of sanga males (crossbreed Bos taurus x Bos indicus, n = 263) and 95% of zebu x sanga crossbred males (n = 56) had the indicine allele. The Borgou, a breed classified as zebu x taurine cross showed only the zebu allele (n = 12). The indicine allele dominates today in the Abyssinian region, a large part of the Lake Victoria region and the sahelian belt of West Africa. All the sanga males (n = 64) but only one from the Abyssinian region had the indicine allele. The taurine allele is the commonest only among the sanga breeds of the southern African region and the trypanotolerant taurine breeds of West Africa. In West Africa and in the southern Africa regions, zones of introgression were detected with breeds showing both Y chromosome alleles. Our data also reveal a pattern of male zebu introgression in Mozambique and Zimbabwe, probably originating from the Mozambique coast. The sanga cattle from the Lake Victoria region and the Kuri cattle of Lake Chad, cattle populations surrounded by zebu breeds were, surprisingly, completely devoid of the indicine allele. Human migration, phenotypic preferences by the pastoralists, adaptation to specific habitats and to specific diseases are the main factors explaining the present-day distribution of the alleles in sub-Saharan Africa.

Africa↗

Breeding origin and migration pattern of dunlin (Calidris alpina) revealed by mitochondrial DNA analysis.

The large-scale migration of birds has been studied extensively by recoveries of ringed birds. However, there is very little ringing data from the arctic breeding grounds of waders. Here, the migration pattern of the dunlin, Calidris alpina, is studied with population genetic markers, using haplotype frequencies to estimate the breeding origin of migrating and wintering populations. Polymerase chain reaction (PCR) and restriction analysis of DNA from the mitochondrial control region was used to study the breeding origins of morphologically similar winter populations in the western Palaearctic, and to describe the population structure of the dunlin during winter. Also migrating dunlin from various stopover sites in Europe, Africa and Asia, were analysed with respect to their mitochondrial DNA (mtDNA) haplotypes. The genetic markers clearly show that the dunlin has a parallel migration system, with populations breeding in the western Palaearctic wintering mainly in the western part of the wintering range, and dunlin populations breeding further east wintering further east. The results also show that the distance between breeding and wintering area increases eastwards in this region.

Animals↗

Factors affecting the reproductive efficiency of artificial insemination programmes in a seasonal breeding pasture-based dairy system with the aid of milk progesterone.

To determine the factors affecting reproductive efficiency in a seasonal breeding artificial insemination (AI) programme in dairy herds in Uruguay, a field trial was conducted on three dairy farms. Lactating cows (n=328) intended to be bred at the following breeding period were selected. The trial started 1 week before the beginning of the breeding period and lasted 80 days or until first service. Milk samples were obtained biweekly for 12 weeks, starting 1 week prior to the breeding season to determine progesterone concentrations. Data indicated that 12.5% of the cows were anoestrus at the beginning of the season remaining in this condition throughout the trial. Most of the anoestrous animals were first-calf heifers. A varying number of cycling cows (8.5%) at each farm were never reported in heat. Oestrous detection rate for cows cycling, as determined by progesterone patterns, was evaluated in three periods of 21 days and resulted in an overall efficiency of 46.9%. Main factors affecting oestrous detection were farm, with an effect of parity (67.8%, mature cows and 33.2%, first-calf heifers) and no effect of days postpartum. Mean interval from the beginning of the breeding season to first breeding was 27.4 days, which was also affected by farm. Neither parity nor days postpartum affected this parameter.

Anestrus↗

The sustainability, feasibility and desirability of breeding livestock for disease resistance.

Selective breeding for disease resistance utilises proven animal breeding methods to improve animal health, welfare and productivity. Unsurprisingly, it is receiving more and more attention from livestock breeders. However, there are a number of largely theoretical arguments that have been raised as potential problems in selection for disease resistance. These can be classified under sustainability, feasibility and desirability. This review considers each of these areas in turn. Several examples show that enhanced resistance to disease is stable under natural selection and therefore deliberate selection for disease resistance should also be stable and sustainable. The feasibility of selective breeding depends in part upon the heritability of the trait or traits used to measure disease resistance, as well as the amount of variation among animals. The heritability of traits associated with resistance to many important diseases is often high and considerable variation among animals exists. Consequently, selective breeding for enhanced disease resistance is certainly feasible. The desirability of breeding for disease resistance depends upon whether there are trade-offs with other economically important traits. By and large these remain to be defined. However, even if unfavourable associations exist, breeders can create selection indices that include traits with unfavourable associations and maximise the desired responses while attempting to minimise undesirable effects. In conclusion, so long as one or more diseases exert a significant influence on livestock production, selective breeding will be a useful tool to assist in disease control.

Animals↗

Evidence for propofol hydroxylation by cytochrome P4502B11 in canine liver microsomes: breed and gender differences.

1. The study aimed to ascertain the enzyme kinetic basis for breed differences in the biotransformation of propofol in dog and to identify the responsible canine cytochrome P450 (CYP) isoenzymes. 2. The NADPH-dependent formation of 4-hydroxypropofol (the rate-limiting biotransformation in dog) was assayed using hepatic microsomes from the male greyhound and beagle, and from both sexes in mixed-breed dogs (five of each). 3. Enzyme kinetic analysis revealed that whereas there were no significant differences in Km, Vmax averaged > 3-fold lower in greyhound compared with beagle (p = 0.032). Although average Vmax was > 3-fold higher in the male compared with female mixed-breed dogs, this difference did not achieve statistical significance (p = 0.095), probably because of the high variability of data from mixed-breed dogs. 4. Chloramphenicol (a specific CYP2B11 inhibitor) and diethyldithiocarbamate (a non-specific CYP2 inhibitor) inhibited propofol hydroxylation in all microsomes. Quinine (a CYP2D15 inhibitor) was also inhibitory, but only in one-half of the microsomes examined. Immuno-inhibition by anti-CYP2B1 sera resulted in > 50% reduction in metabolite formation in all dogs except mixed-breed females, which showed a 30% reduction. Differences in propofol hydroxylase activity between microsomal preparations were primarily attributed to a component that was sensitive to inhibition by chloramphenicol and anti-CYP2B1 sera. 5. The results indicate that propofol hydroxylation in dog is primarily mediated by CYP2B11 and that breed (and possibly gender) differences in propofol metabolism may result from differences in the liver content of this CYP.

Animals↗

Genetic parameters for egg number, egg weight, and eggshell color in three Catalan poultry breeds.

Heritabilities for egg number, egg weight, and eggshell color (percentage light absorbance) at 39 wk of age, and the genetic correlations between them were estimated by restricted maximum likelihood in three Catalan poultry breeds: Penedesenca Negra (PN), Prat Lleonada (PL), and Empordanesa Roja (ER). Additive genetic differences between these breeds were also estimated. Data were from the IRTA Poultry Genetic Conservation Program and consisted of records from 1,309 PN, 1,466 PL, and 1,440 ER hens, which were obtained from 80 contemporary batches per breed hatched between 1987 and 1992. Estimates of heritability for egg number, egg weight, and eggshell color were, 0.20, 0.59, and 0.49 for PN, 0.31, 0.48, and 0.53 for PL; and 0.33, 0.50, and 0.27 for ER. Estimated genetic correlations between egg number and egg weight, egg number and shell color, and egg weight and shell color were, for PN, -0.22, -0.03, and 0.00; for PL, -0.21, -0.06, and 0.09; and -0.19, -0.29, and 0.30 for ER. Heritability for eggshell color and genetic correlation between eggshell color and other traits showed a different genetic pattern in ER breed. Significant additive genetic differences (P < 0.05) were found between ER and PN base populations for egg number (3.89), egg weight (0.91), and eggshell color (-3.50); and between ER and PL for egg number (6.69) and eggshell color (35.39). The PN and PL breeds differed significantly (P < 0.05) for eggshell color (38.22), which was darker in PN. These results could be taken as the expected genetic differences for these breeds.

Aging↗

Coalitions of relatives and reproductive skew in cooperatively breeding white-winged choughs.

We used DNA fingerprinting to examine reproductive skew in cooperatively breeding white-winged choughs, Corcorax melanorhamphos, which live in groups of up to 20 individuals. Before a severe drought, groups that had been stable for multiple years were characterized by long-term monogamy involving a single breeding pair (high skew). After the drought, new groups formed from the amalgamation of multiple individuals and coalitions of relatives. At most one member of each faction succeeded in breeding, such that skew was dependent on the number of unrelated factions, and not group size. In the new groups, dominant males and females with supporting relatives were always successful. Whereas most females without support also gained breeding positions, many males without family support failed to breed. Thus subordinates gain indirect fitness by first helping related males to secure a breeding position, and then helping to raise their young. Our study demonstrates the advantage of operating in coalitions, and suggests that the acquisition of future allies may be a major benefit of helping behaviour in this species.

Animals↗

Using expected allele number as objective function to design between and within breed conservation of farm animal biodiversity.

Conservation of genetic diversity in farm animal species can be achieved by preventing extinction of breeds and by reducing genetic drift within breeds. It is suggested to use the expected number of alleles segregating in the species after a given time period as objective function in the design of conservation strategies. A formal approach is presented to predict this quantity based on marker information, accounting for extinction probability of breeds and effective population size within breeds as the major component of genetic drift. Based on this model, relative efficiency of different strategies of diversity conservation can be quantified. Formulas are given to derive the marginal expected number of alleles with respect to genetic drift within population and extinction probability, respectively. The suggested approach is illustrated with an example of 13 European cattle breeds. With the assumed parameters, drift is shown to be the major force leading to loss of alleles, and different breeds are prioritized for activities to reduce risk of extinction and for measures to reduce genetic drift, respectively. Although different aspects of the model need to be further refined, the suggested methodology provides a general and flexible tool to derive the optimum conservation strategy in various scenarios.

Alleles↗

Social and breed effects on the expression of a PGF2alpha induced oestrus in beef cows.

Social organization and breed effects following PGF2alpha were studied in mature Angus, Brahman and Senepol cows allocated into two groups (each A = 5, B = 5 and S = 5). Variables including interval to oestrus onset (IEO), oestrous duration (DE), total mounts received (TMR), and oestrous intensity (IE) were derived via HeatWatch. Breed-type influenced IEO (B = 42.6 +/- 6.7 h; S = 54.6 +/- 6.0 h; and A = 27.8 +/- 5.8 h; p < 0.003). Within breeds, dominant B (69.4 +/- 13.3 h) and S (65.5 +/- 7.4 h) cows were slower (p < 0.05) to be detected in oestrus than subordinate (38.1 +/- 4.4 h) and intermediate (40.6 +/- 6.0 h). However, within A, dominant cows (16.4 +/- 12.5 h) were detected in oestrus earlier (p < 0.05) than intermediate (44.3 +/- 9.2 h) and subordinates (32.7 +/- 5.1 h). Angus (21.5 +/- 2.4 h) and B (22.1 +/- 3.0 h) cows had longer (p < 0.01) DE than S (9.1 +/- 2.8 h). Dominants (20.4 +/- 3.0) and intermediates (20.2 +/- 2.3 h) cows had longer DE (p < 0.04) than subordinates (12.1 +/- 2.1 h) although the interaction breed x social order showed that dominant S had shorter DE than dominant A and B (10.1 +/- 3.3; 34.8 +/- 6.0 h; and 20.0 +/- 6.4 h, respectively; p < 0.001). Angus cows had less TMR than B (p < 0.02) and tended to be less than S cows (p < 0.06). Overall, greatest (p < 0.008) IE occurred in the first 9 h after onset of oestrus with no breed effect (p > 0.05). Dominant cows tended (p < 0.10) to have less TMR (3.2 +/- 0.7 mounts) than subordinate (4.1 +/- 0.4 mounts) and intermediate (4.7 +/- 0.6 mounts) throughout, especially 3-6 h after oestrus onset (p < 0.07). Breed and social order both influence PGF2alpha-induced oestrus behaviour.

Animals↗

The development and application of the modern reproductive technologies to horse breeding.

Although the horse was probably the first animal to experience and benefit from artificial insemination, it trailed the field somewhat with regard to the application of embryo transfer and other oocyte and embryo-related modern breeding technologies. But with a late run it is now back in mid-field and gaining fast on the other large domestic species in the application of the many technological advances of the past 20 years to sound breeding practice. Improvements in extenders and cryoprotectants have resulted in a veritable upsurge in the transport and insemination of cooled and frozen stallion semen, and parallel improvements in ovulation induction and synchrony, exogenous gonadotrophic stimulation of multiple fertile ovulations and simplified, more efficient methods for non-surgical transfer of embryos to recipient mares, coupled with relaxation of breed society registration restrictions, have together contributed to a similar upsurge in the application of embryo transfer to all breeds and athletic types of horses worldwide, with the continuing and notable exception of the Thoroughbred. Although conventional in vitro fertilization remains something of an unjumped fence in equids, other modern breeding technologies like hysteroscopic low-dose insemination, fluorescence-activated sex sorting of stallion spermatozoa, between-species embryo transfer, embryo freezing and bisection, transvaginal ultrasound-guided oocyte collection, intracytoplasmic sperm injection for fertilization (ICSI), gamete intrafallopian transfer (GIFT) and now nuclear transfer (cloning), have all been applied to equids with encouraging success. Cloning, especially, holds enormous promise for the Sporthorse industry to re-create champion geldings in stallion form for breeding purposes.

Animals↗

Effect of training duration and exercise on blood-borne substrates, plasma lactate and enzyme concentrations in Andalusian, Anglo-Arabian and Arabian breeds.

Metabolic responses to exercise differ between Andalusian horses and other breeds, although changes in plasma muscle enzymes have not been reported and most useful information is obtained from animals subjected to different training programmes. The objectives of this study were to 1) describe the changes in plasma enzymes during exercise in different horse breeds in relation to other biochemical parameters (Experiment A) and 2) assess the effect of training duration on these measures (Experiment B). Twenty stallions, 9 Andalusian (AN), 7 Arabian (A) and 4 Anglo-Arabian (AA), age 5-10 years, were studied. They performed 3 exercise tests (ET), consisting of a warm-up of 800 m at 0.7 km/h and 4 workloads at 15, 20, 25 and 30 km/h, at respective distances of 1250, 1670, 2080 and 2500 m, with 5 min active recovery between each workload (Experiment A). Three ETs were performed at the beginning and after 2 and 6 months of training (Experiment B). Venous blood samples were collected during the ETs and plasma glucose (GLU), free fatty acids (FFA), lactate (LA), creatine kinase (CK), lactate dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (HBHD), aspartate aminotransferase (AST), Na+, K+ and Cl- were measured. AN horses responded to exercise with greater increases in GLU, HBHD, LDH, CK and AST compared to the other breeds. An unexpected result in Experiment A was the lack of interbreed differences in plasma peak LA concentrations, since it is commonly accepted that AA and A horses have greater athletic potential. Although the glycolytic response to exercise was reduced after 2 months of training in the AA and A horses, and after 6 months of training in the AN horses, at the end of Experiment B, AN horses produced more lactate than the other 2 breeds. Most of the adaptations linked to training were found in the AN breed. The more striking changes in plasma enzyme activities corresponded to CK in AN horses after 2 months of training. The attenuation of CK response to exercise was related to lower extrafibrilar GLU utilisation with LA formation and greater fat metabolism. The results show that plasma muscle enzyme concentrations for the diagnosis of equine myopathies must be interpreted in relation to breed and training.

Animals↗

Comparison between estimation of breeding values and fixed effects using Bayesian and empirical BLUP estimation under selection on parents and missing pedigree information.

Bayesian (via Gibbs sampling) and empirical BLUP (EBLUP) estimation of fixed effects and breeding values were compared by simulation. Combinations of two simulation models (with or without effect of contemporary group (CG)), three selection schemes (random, phenotypic and BLUP selection), two levels of heritability (0.20 and 0.50) and two levels of pedigree information (0% and 15% randomly missing) were considered. Populations consisted of 450 animals spread over six discrete generations. An infinitesimal additive genetic animal model was assumed while simulating data. EBLUP and Bayesian estimates of CG effects and breeding values were, in all situations, essentially the same with respect to Spearman's rank correlation between true and estimated values. Bias and mean square error (MSE) of EBLUP and Bayesian estimates of CG effects and breeding values showed the same pattern over the range of simulated scenarios. Methods were not biased by phenotypic and BLUP selection when pedigree information was complete, albeit MSE of estimated breeding values increased for situations where CG effects were present. Estimation of breeding values by Bayesian and EBLUP was similarly affected by joint effect of phenotypic or BLUP selection and randomly missing pedigree information. For both methods, bias and MSE of estimated breeding values and CG effects substantially increased across generations.

Alleles↗

Genotyping of beta-lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cattle breeds.

BACKGROUND: Improvement of efficiency and economic returns is an important goal in dairy farming, as in any agricultural enterprise. The primary goal of dairy industry has been to identify an efficient and economical way of increasing milk production and its constituents without increasing the size of the dairy herd. Selection of animals with desirable genotypes and mating them to produce the next generation has been the basis of livestock improvement and this would continue to remain the same in the coming years. The use of polymorphic genes as detectable molecular markers is a promising alternative to the current methods of trait selection once these genes are proven to be associated with traits of interest in animals. The point mutations in exon IV of bovine beta-Lactoglobulin gene determine two allelic variants A and B. These variants were distinguished by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) analysis in two indigenous Bos indicus breeds viz. Sahiwal and Tharparkar cattle. DNA samples (228 in Sahiwal and 86 in Tharparkar) were analyzed for allelic variants of beta-Lactoglobulin gene. Polymorphism was detected by digestion of PCR amplified products with Hae III enzyme, and separation on 12% non-denaturing gels and resolved by silver staining. RESULTS: The allele B of beta-Lactoglobulin occurred at a higher frequency than the allele A in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in Sahiwal and Tharparkar breeds were 0.031, 0.276, 0.693 and 0.023, 0.733, 0.244 respectively. Frequencies of A and B alleles were 0.17 and 0.83, and 0.39 and 0.61 in Sahiwal and Tharparkar breeds respectively. The Chi-square test results (at one degree of freedom at one per cent level) revealed that the Tharparkar population was not in Hardy-Weinberg equilibrium as there was a continuous migration of animals in the herd studied, where as, the results are not significant for the Sahiwal population. CONCLUSION: Genotype frequencies of AA were the lowest compared to that of BB genotype in Sahiwal cattle while AB genotypes were more frequent in Tharparkar cattle. The frequency of A allele was found to be lower than that of B allele in both the breeds studied. These results further confirm that Bos indicus cattle are predominantly of beta-Lactoglobulin B type than Bos taurus breeds.

Alleles↗

Effect of lamb age, breed and sex on plasma LH after administration of GnRH.

Male lambs of the Finn, Fingalway and High Fertility breeds aged 4 or 8 weeks of age were injected i.m. with 5, 10, 50, or 100 micrograms GnRH. Plasma LH after GnRH injection increased with age and GnRH dose but there was no effect of breed. There was no interaction among the three factors studied. In a second experiment the response of male and female lambs, aged 8 weeks, to 100 micrograms GnRH was investigated for 5 genetic groups (Finn, Galway Control, Galway Elite, Fingalway and High Fertility). Breed differences in plasma LH of male lambs were not found, which agreed with the result of the first experiment. Breed differences were observed for females, with the lowest values being observed for Finn and High Fertility lambs which represent the two most prolific breeds. This finding suggested breed differences in the development of the hypothalamic-pituitary-ovarian circuit. The difference between Galway Control and Galway Elite lambs was not significant (P greater than 0.10).

Age Factors↗