[Autolysis and regeneration of the culture of acid fast bacilli].
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The character of the cell membrane component destruction of the human epidermis when kept in a humid camera at 4 degrees or 37 degrees C was studied electron microscopically and by a freezing-chopping method. Intermembranous proteins undergo considerable changes and are more labile than a lipid membranous component.
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More than 100 various fractions were detected in human skeletal muscle proteins analyzed by O'Farrell two-dimensional electrophoresis. Main proteins were maintained well in individual preparations of muscle tissue after their storage within 4 days at 20 degrees. The protein fractions with molecular mass of 140-150 kD as well as alteration of other muscle proteins were found on electrophoregramms within 6 days of storage.
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Ultrastructure of the myocardium in the experiment was studied just after the heart ventricular fibrillation and 3 h after the animal death; the results were compared to those obtained by studying ultrastructure of human myocardium in patients dying from myocardial infarction complicated by ventricular fibrillation. The similarity of ultrastructural and autolytic changes was revealed in both series of observation. Overcontraction of myofibrils and redistribution of the mitochondria in cardiomyocytes, increase of the sarcoplasmic reticulum vesiculation, oedema of sarcoplasm, structural alterations of intercalative discs, later appearance of the symptoms of the irreversibility of lesions in the mitochondria and early penetration of colloidal lanthanum particles in rigor cells may serve ultrastructural diagnostic symptom of ventricular fibrillation. A better preservation of Purkinje's cells as compared to the contractile cardiomyocytes is also demonstrated.
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