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Results for “quantitative analysis”

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At least 559 records · Page 31Linked to original sources
PubMed2004

[Quantitative analysis of Epstein-Barr virus DNA in plasma and peripheral blood cells in patients with nasopharyngeal carcinoma].

OBJECTIVE: To quantitative analysis of Epstein-Barr virus (EBV) DNA levels in plasma, peripheral blood cells (PBCs) and tumor tissue in nasopharyngeal carcinoma (NPC), to investigate the relationship between EBV-DNA levels and clinical parameters. METHODS: Blood of 150 primary NPC and 49 corresponding tumor tissues, 47 nasopharyngitis tissues and blood of 75 controls were entered this investigation. Plasma and PBCs were isolated for quantitative detection of EBV-DNA by using real-time quantitative PCR (RQ-PCR). The paraffin-embedded tissue sections were conducted to quantitative detection of EBV-DNA, and EBER1 in situ hybridization (ISH) for calculating the percentage of positive cells on the tissue section. RESULTS: Plasma EBV-DNA levels and detecting rate in NPC before treatment (median 82 500 copies/ml, 92%) were significantly higher than that in NPC after treatment (median 0 copy/ml, 19%) and in controls (median 0 copy/ml, 12%) (P < 0.05), whereas there was no significant difference between NPC after treatment and controls (P > 0.05). There was no significant difference of PBCs EBV-DNA load and detecting rate in NPC before (0 copy/actin, 24%) and after treatment (0 copy/actin, 14%), as well as in controls (0 copy/actin, 16%) (P > 0.05). Plasma EBV-DNA level was not correlated to PBCs EBV-DNA load in NPC before (P = 0.92) and after treatment (P = 0.27), and controls (P = 0.74). EBV-DNA level (27.8 copies/actin) in NPC tumor tissues was significantly higher than that in nasopharyngitis (0 copy/actin) (P < 0.05), and was positively correlated to the ratio of EBER1 positive cells to total cells on the NPC section. In NPC patients, plasma EBV-DNA level was significantly increased in TNM stage I (2500 copies/ml), II (32 590 copies/ml), III (86 000 copies/ml) and IV (166 200 copies/ml), whereas there was no significantly difference of PBCs EBV-DNA loads in difference stages of NPC. CONCLUSION: Plasma EBV-DNA level is a more sensitive and reliable biomarker than PBCs EBV-DNA loads for reflection the tumor volumes in NPC patients. Plasma EBV-DNA detection will improve TNM staging in NPC clinical practice on molecular level.

Biomarkers, Tumor↗
PubMed2000

Quantitative analysis of estrogen receptor-beta mRNA and its variants in human breast cancers.

We have carried out a quantitative analysis of ER-alpha and ER-beta mRNA expression in normal (n = 11) and breast cancer (n = 112) tissues using a real-time (Taq-Man) PCR assay. Expression of ER-beta mRNA variants has also been studied by triple-primer PCR assay. ER-alpha mRNA levels in normal breast tissues were significantly (p < 0.01) lower than those in ER-positive breast cancers but not significantly different from those in ER-negative breast cancers. However, ER-beta mRNA levels in normal breast tissues were significantly (p < 0.01) higher than those in ER-positive and ER-negative breast cancers. Proportions of ER-beta1 and ER-beta2 mRNA expression among total ER-beta mRNA expression were significantly higher and those of ER-beta5 and ER-beta5; mRNA were significantly lower in normal breast tissues than in ER-positive and ER-negative breast cancers. ER-beta mRNA levels and proportions of ER-beta mRNA variants did not show any significant correlation with age, tumor size, lymph node status and histological grade. Our results demonstrate that ER-alpha mRNA is up-regulated and ER-beta mRNA is down-regulated during carcinogenesis of breast cancers. Changes in proportions of ER-beta mRNA variants are also implicated in this process.

Adult↗
PubMed1985

A method for quantitative analysis of ratios of types I and II collagen in small samples of articular cartilage.

Currently available methods for quantitative analysis of type II collagen in studies of articular cartilage repair either require much larger samples than are available or are inaccurate and unreliable. A method of determining the percentage of type II collagen in small samples of articular cartilage (100 to 200 micrograms) by measuring the spectrophotometric densities of specific cyanogen bromide peptide bands from mixtures of types I and II collagen on sodium dodecyl sulfate-polyacrylamide gels has been developed and found to be accurate and very reliable. The ratio of the area under the alpha 1(II)CB10 peak to the area under the alpha 1(I)CB7,8 + alpha 1(II)CB11 peak was function of the proportion of type II collagen in the sample. Since the ratio was independent of the quantity of sample loaded onto the gel, it was not affected by moderate losses of sample. This method should therefore be useful in the fields of collagen research and particularly valuable to those investigating the repair and regeneration of articular cartilage.

Animals↗
PubMed2005

Physical properties of root cementum: Part 4. Quantitative analysis of the mineral composition of human premolar cementum.

BACKGROUND: The aim of this study was to perform a quantitative analysis of the calcium (Ca), phosphorus (P), and fluoride (F) concentrations in human first premolars. METHODS: This study was conducted on 18 maxillary or mandibular first premolars that were collected from 16 prospective orthodontic patients (10 male, 6 female), mean age 13.9 years (range, 11.7-16.1 years), requiring first premolar extractions. After extraction, the teeth were prepared for electron probe microanalysis. The Ca, P, and F concentrations were measured on the buccal and lingual surfaces at the midpoint of the cervical, middle, and apical thirds of the root from the outer to middle to inner third of the cementum. RESULTS AND CONCLUSIONS: In first premolar cementum, there was significant interindividual variation in the Ca, P, and F concentrations ( P = .024, .017, and .000, respectively). There was no significant difference in the Ca, P, and F concentrations of cementum between the buccal and lingual surfaces, except for a significantly higher F content at the cervical region on the buccal surface ( P = .000). There was a decreasing gradient in the Ca, P, and F concentrations from the cervical to the apical third of the root, which was highly significant from the cervical to middle third ( P = .000) and from the middle to apical third ( P = .000), except for F, for which there was no significant difference from the cervical to the middle third on the lingual surface ( P = .966). There was a significant increasing gradient in the Ca and P concentrations from the outer to inner third of cementum at the cervical ( P < .01) and middle ( P < .01) thirds of the root but no significant difference at the apical third of the root. For F, there was a significant decreasing gradient from the outer to the inner third of cementum at the cervical ( P < .01), middle ( P < .01) and apical ( P < .01) thirds of the root.

Adolescent↗
PubMedDate not supplied

Quantitative analysis of tryptic protein mixtures using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.

For the first time, quantitative analysis of tryptic protein mixtures, labeled with Quantification-Using-Enhanced-Signal-Tags (QUEST)-markers, were performed with electrospray ionization and a 9.4 T Fourier Transform Ion Cyclotron Resonance (FTICR) mass spectrometer. Coupling a High-Pressure Liquid Chromatography (HPLC) separation step prior to mass analysis resulted in an increased amount of identified labeled tryptic peptides. The range for the determined intensity ratios of two peptides in a labeled pair was large, but the obtained median intensity ratio correlated very well with the corresponding concentration ratio. This method can be used for observing protein dynamics in a specific cell type, tissue, or in body fluids.

Animals↗
PubMed1988

Experimental study of vascularized bone: quantitative analysis of bone scintigraphy and histology.

The purpose of this study was the sequential evaluation of vascularized bone by quantitative analysis of bone scintigraphy with Tc-99m methylene diphosphonate (MDP). The correlation between the uptake of Tc-99m MDP and the morphologic behavior of vascularized bone was investigated, simultaneously with bone labeling, contact microradiogram (CMR), and histology of an undecalcified sample. In this experiment, 41 adult female rabbits were used. An island tibia pedicled with the popliteal vessels was used as the model of vascularized bone. The maximum uptake of Tc-99m MDP was observed at two to four postoperative weeks. When bone marrow was partially exposed, the uptake was significantly large. On the basis of this experimental study, the degree of Tc-99m MDP uptake seemed to depend primarily on the osteoblastic activity of vascularized bone. The newly-formed bone was observed mainly at periosteal sites.

Animals↗
PubMed1985

A sensitive method for quantitative analysis of phospholipid molecular species by high-performance liquid chromatography.

A simple and sensitive method was developed for quantitative analysis of phospholipid molecular species. Diacylglycerols were prepared from phospholipids by phospholipase C treatment and converted to the corresponding dinitrobenzoyl derivatives, which could be sensitively detected at 254 nm. The derivatives of 21 molecular species were resolved by high-performance liquid chromatography with an octadecylsilyl reversed-phase column. All the derivatives had the same peak area per mol, and peak areas were proportional to the amounts of the derivatives. Quantification was carried out at the picomole level.

Chromatography, High Pressure Liquid↗
PubMed1983

Quantitative analysis of tremor in Minamata disease.

Applying the techniques of power spectrum and pulse correlation, we carried out a quantitative analysis of tremor in normal subjects, patients with methyl mercury poisoning (Minamata disease) and patients with other diseases. We found that tremor of methyl mercury poisoning was different from physiological tremor and the other pathological tremors in frequency and amplitude.

Adolescent↗
PubMed2001

[Quantitative analysis of cell fusion caused by glycoprotein of paramyxoviruses with reporter gene method].

OBJECTIVE: To establish a kind of quantitative assay method for analyzing the reporter gene assay for fusion was established. METHODS: Two populations of BHK21 cells were infected with vTF-7 recombinant and wild-type vaccinia viruses respectively and transfected with paramyxovirus F and HN cDNA and plasmid pG1NT7 beta-gal respectively. After 16 h, the two cell populations were removed from the wells by trypsinization. Equal numbers of the two populations were mixed in wells of a 96-well flat-bottom plate after washing and pelleting. After 15 hours' incubation at 37 degrees C, the cells were lysed with Nonidet P-40 and A values were read at 570 nm with an ELISA reader supported by SOFTMAX software. RESULTS: Reporter gene method had a close relationship with cell count method, r = 0.9890(P < 0.01). Positive and negative coincidence rates were 100%. The best main reaction conditions are as follows: substrate concentration, 16 mmol/L; color reaction time, 20-25 min; amount of DNA for transfection, 1 microgram; cell numbers for each population, 1 x 10(5). CONCLUSION: Reporter gene method is a kind of very sensitive, specific and repeatable quantitative analysis for cell fusion and can be used in the studies on membrane fusion caused by any viruses.

Cell Fusion↗
PubMed1992

[Quantitative analysis of the isthmo-optic nucleus and projection neurons to the retina in adult fowl (Gallus gallus domesticus)].

Quantitative analysis of the isthmo-optic nucleus (IO) and centrifugal projection to the retina in the fowl was made using Nissl preparation and retrograde horseradish peroxidase (HRP) methods. Seven adult fowls (Gallus gallus domesticus) were used for Nissl stain. Serial sections were cut on a freezing microtome at 60 microns and stained with cresyl violet. IO was situated just medial to the caudal part of the tectum and laterodorsal surface of the brain stem. Rostrocaudal extension of IO was about 800-1,000 microns. The average total volume and neuronal population of the IO was 280 x 10(-3) mm3 and 5,600 neurons, respectively. Eight animals were used for HRP study. One hundred microliters of 30% HRP solution in physiological saline was injected into the vitreous body of one eye of each hen. Serial transverse sections of 60 microns were treated with tetramethyl benzidine (TMB). Many labeled neurons were found in contralateral brain stem. Average total number of contralateral HRP-labeled cells in IO and peri-IO were 5,268 and 1,492, respectively. Labeled neurons peri-IO were mainly distributed ventrally and rostrally to IO. No labeled neurons in IO, and only a few labeled neurons peri-IO were found ipsilaterally. The number of HRP-labeled neurons in IO corresponded to the neuronal population of IO in Nissl preparation, which suggested that most of isthmo-optic neurons might be projecting to the contralateral retina. In contrast to the round and small IO neurons (long axis 15-20 microns, short axis 10-20 microns), peri-IO neurons were multipolar and longer (long axis 15-30 microns, short axis 10-25 microns).

Animals↗
PubMed1992

[A quantitative analysis of ataxia in the upper limbs].

By using a transparent digitizer and a personal computer, we tried a quantitative analysis of ataxia in the upper limbs. A total of 25 upper extremities of 13 patients with spino-cerebellar degeneration (SCD) and 140 upper extremities of 70 normal volunteers were tested by two types of tasks. One was the free speed trace of a circle presented on the display (free circle), and the other was the pursuit of a target moving on a circle at a fixed speed (pursuit circle). The digitizer was put on a color display stabilized horizontally. For 1024 points with a 25 msec sampling time, the trajectory of the stylus pen was transmitted to the computer in real time. Circle diameters of 3 cm and 6 cm were selected. The target rounded the circle at a speed of 6 or 3 times per 1024 points. The mean velocity (MV), mean acceleration (MA), and MA/MV ratio were calculated for the extent of one circle period. And the coefficient of variation (CV) of the shifted distance in each sampling time and the power spectrum by Fast Fourier Transform (FFT) to the acceleration wave were calculated for the total input data. In the free circles, the MV and logarithm of MA varied widely and showed positive correlations. In contrast, those of the pursuit circles converged narrowly near the values of the target. On the other hand, in the SCD group, many patients could not draw the small free circle.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[A quantitative analysis of regional features in the structure of aortal endothelial cells in short-term hypertension].

The results of quantitative analysis of regional differences in the structure of thoracic aorta endotheliocytes in normal and hypertensive rats are presented. Scanning electron microscopy and semiautomatic image analyser were employed in studying the cellular structure in three regions of aorta endothelial layer: dorsal and ventral surfaces and region of the intercostal arterial ostia. The data obtained show that in cases of a short-duration hypertension the most pronounced are the alterations in endotheliocytes of the dorsal surface. Thus, the results confirm the heterogeneity of the aortic endothelium under normal conditions and demonstrate that differences in overlapping of neighbouring endotheliocytes is the cause of the observed alterations in cases of hypertension.

Animals↗
PubMedDate not supplied

[Quantitative analysis of the polymorphism of the C-bands of chromosomes 1, 9, 16 and Y in Latvians].

A quantitative analysis of the C-band polymorphism in chromosomes 1, 9, 16 and Y was made in 50 phenotypically normal individuals (25 males and 25 females). At an average level of the chromosome spiralization (the mean chromosome size is 2-7 micrometers) mean C-band lengths of chromosomes 1, 9, 16 and Y obtained in the course of the study are 1.09, 0.94, 0.82 and 0.80 micrometers, respectively. A comparative analysis of the mean C-band lengths in chromosomes 1, 9, 16 in the male and female groups has revealed no sex differences. The highest variability of the C-band size is found for chromosome 1 and the lowest--for chromosomes 16 and Y.

Adolescent↗
PubMedDate not supplied

Studies of quantitative analysis of protein expression in Saccharomyces cerevisiae.

In the present study amino acid analysis is applied to quantitation of Saccharomyces cerevisiae proteome expression. The quantitation levels obtained are compared to data using densitometric analysis of silver or amido black staining and to the theoretical expression level (codon bias) of the identified proteins determined from their amino acid analysis (AAA). The results show that relative volume ratio (%vol) using Melanie II is a better parameter for spot quantitation than relative optical density ratio (%OD), and amino black staining provides good linearity within the range 1-100 pmol protein. However, AAA shows that theoretical expression levels are not well correlated with actual protein expression level, although there is better correlation when isoforms of the expressed protein are identified and included. It is concluded that amino acid analysis provides accurate protein quantitation and has a continuing role in proteome studies in terms of the rapid and inexpensive quantitation of proteins displayed on proteome maps. We do however recognize that in the context of future clinical applications and large-scale proteome discovery projects, quantitation and post-translational modification need to be analyzed by 'proteomatic' (i.e., proteome automatic bioinformatic analysis directly from the gel) techniques.

Electrophoresis, Gel, Two-Dimensional↗
PubMed2006

[Quantitative analysis of two-component polymer blends (PEG/PE) by infrared spectroscopy].

The concentration of the camponent of PEG/PE blends was analyzed quantitatively by infrared spectroscopy. The absorption peak area ratio of the selected mixture peaks used as the calibrating basis for the quantitative analysis was more reasonable than the peak area ratio of the pure peaks. The theoretical equation deducted by Beer-Lamber law was used to establish the working curve to calculate the composition of the responding functional groups in the film of the PEG/PE blends. The characteristic peaks of the crystal can not be selected as the calibrating basis for the quantitative measurement, because the crystallization has a great effect on the intensity of the absorption peaks.

English Abstract↗
PubMed1979

Chlorpheniramine. I. Rapid quantitative analysis of chlorpheniramine in plasma, saliva and urine by high-performance liquid chromatography.

A method was developed for the rapid quantitative analysis of chlorpheniramine in plasma, saliva and urine using high-performance liquid chromatography. A diethyl ether or hexane extract of the alkalinized biological samples was extracted with dilute acid which was chromatographed on a reversed-phase column using mixtures of acetonitrile and ammonium phosphate buffer as the mobile phase. Ultraviolet absorption at 254 nm was monitored for the detection and brompheniramine was employed as the internal standard for the quantitation. The effects of buffer, pH, and acetonitrile concentration in the mobile phase on the chromatographic separation were investigated. A mobile phase 20% acetonitrile in 0.0075 M phosphate buffer at a flow-rate of 2 ml/min was used for the assays of plasma and saliva samples. A similar mobile phase was used for urine samples. The drug and internal standard were eluted at retention volumes of less than 17 ml. The method can also be used to quantify two metabolites, didesmethyl- and desmethylchlorpheniramine, in the urine. The method can accurately measure chlorpheniramine levels down to 2 ng/ml in plasma or saliva using 1 ml of sample, and should be adequate for biopharmaceutical and pharmacokinetic studies. Various precautions for using the assay are discussed.

Chlorpheniramine↗